Nile crocodilepox virus (isolate Crocodylus niloticus/Zimbabwe/Ume/2001) (CRV)
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 172 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q070N3|Q070N3_CPRVZ Uncharacterized protein OS=Nile crocodilepox virus (isolate Crocodylus niloticus/Zimbabwe/Ume/2001) OX=1289473 GN=CRV018 PE=4 SV=1
MM1 pKa = 7.59 LEE3 pKa = 4.35 AEE5 pKa = 4.82 RR6 pKa = 11.84 GDD8 pKa = 3.14 AGYY11 pKa = 7.91 FTDD14 pKa = 6.01 GDD16 pKa = 4.22 DD17 pKa = 4.43 VDD19 pKa = 5.67 DD20 pKa = 6.08 GSDD23 pKa = 3.6 PDD25 pKa = 3.85 EE26 pKa = 6.15 DD27 pKa = 3.96 GAADD31 pKa = 3.92 PFEE34 pKa = 5.11 HH35 pKa = 7.19 SIVEE39 pKa = 4.09 YY40 pKa = 11.08 DD41 pKa = 3.71 RR42 pKa = 11.84 FPPLSGIRR50 pKa = 11.84 HH51 pKa = 6.74 DD52 pKa = 4.63 LLCVSITLGPEE63 pKa = 3.72 TRR65 pKa = 11.84 EE66 pKa = 3.67 TLYY69 pKa = 11.11 LRR71 pKa = 11.84 FLNALGAVEE80 pKa = 4.52 TIMRR84 pKa = 11.84 VIPVSALEE92 pKa = 4.0 EE93 pKa = 4.07 PFAYY97 pKa = 9.67 EE98 pKa = 3.78 HH99 pKa = 6.42 QIVLSRR105 pKa = 11.84 EE106 pKa = 3.91 YY107 pKa = 10.3 FSRR110 pKa = 11.84 YY111 pKa = 9.4 AAMDD115 pKa = 3.34 MMACSSPDD123 pKa = 3.33 APDD126 pKa = 3.57 PCAYY130 pKa = 9.42 IFSAAGIDD138 pKa = 3.97 AINAFTASARR148 pKa = 11.84 DD149 pKa = 3.78 PEE151 pKa = 4.42 EE152 pKa = 4.59 EE153 pKa = 4.02 PTATYY158 pKa = 10.92 QFFVV162 pKa = 3.57
Molecular weight: 17.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.91
IPC_protein 3.884
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.592
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.177
Thurlkill 3.719
EMBOSS 3.795
Sillero 3.999
Patrickios 1.1
IPC_peptide 3.859
IPC2_peptide 3.986
IPC2.peptide.svr19 3.896
Protein with the highest isoelectric point:
>tr|Q06ZY5|Q06ZY5_CPRVZ Uncharacterized protein OS=Nile crocodilepox virus (isolate Crocodylus niloticus/Zimbabwe/Ume/2001) OX=1289473 GN=CRV166 PE=4 SV=1
MM1 pKa = 7.33 RR2 pKa = 11.84 THH4 pKa = 7.2 LFLSVFLRR12 pKa = 11.84 FLLPRR17 pKa = 11.84 RR18 pKa = 11.84 PSRR21 pKa = 11.84 ATSCRR26 pKa = 11.84 AAAPRR31 pKa = 11.84 CAPRR35 pKa = 11.84 SRR37 pKa = 11.84 PRR39 pKa = 11.84 SRR41 pKa = 11.84 RR42 pKa = 11.84 PPRR45 pKa = 11.84 PGPPRR50 pKa = 11.84 PGSRR54 pKa = 11.84 SRR56 pKa = 11.84 ACGCRR61 pKa = 11.84 GRR63 pKa = 11.84 RR64 pKa = 11.84 ARR66 pKa = 11.84 LLRR69 pKa = 11.84 LPLPLLPRR77 pKa = 11.84 ARR79 pKa = 11.84 SRR81 pKa = 11.84 LPRR84 pKa = 11.84 PRR86 pKa = 11.84 RR87 pKa = 11.84 PPLAAPALARR97 pKa = 11.84 LPRR100 pKa = 11.84 LPPRR104 pKa = 11.84 ARR106 pKa = 11.84 RR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 RR110 pKa = 11.84 RR111 pKa = 11.84 SRR113 pKa = 11.84 PRR115 pKa = 11.84 PRR117 pKa = 11.84 DD118 pKa = 2.96 RR119 pKa = 11.84 PAARR123 pKa = 11.84 RR124 pKa = 11.84 RR125 pKa = 11.84 APPRR129 pKa = 11.84 TRR131 pKa = 11.84 LPPPPPRR138 pKa = 11.84 CRR140 pKa = 11.84 PSPSRR145 pKa = 11.84 SPPSRR150 pKa = 11.84 RR151 pKa = 11.84 SRR153 pKa = 11.84 PRR155 pKa = 11.84 AASSSPSTPTPPPSSPPLPTRR176 pKa = 11.84 PPPSPRR182 pKa = 11.84 PRR184 pKa = 11.84 RR185 pKa = 11.84 RR186 pKa = 11.84 PRR188 pKa = 11.84 AATATGFAPVLALVLDD204 pKa = 4.31 PAPRR208 pKa = 11.84 SSATRR213 pKa = 11.84 SEE215 pKa = 4.18 EE216 pKa = 3.93 VPRR219 pKa = 11.84 SGRR222 pKa = 11.84 LRR224 pKa = 11.84 RR225 pKa = 11.84 TAFSRR230 pKa = 11.84 SLLLFFLKK238 pKa = 10.41 PPPFARR244 pKa = 11.84 LASLASVPPLAPLARR259 pKa = 11.84 DD260 pKa = 3.86 LVARR264 pKa = 11.84 SAPPPSAFPSSLAGKK279 pKa = 9.17 GNRR282 pKa = 11.84 GEE284 pKa = 4.27 ARR286 pKa = 11.84 ATASRR291 pKa = 11.84 AFASVRR297 pKa = 11.84 PASLARR303 pKa = 11.84 RR304 pKa = 11.84 LLPPPSRR311 pKa = 11.84 PRR313 pKa = 11.84 SRR315 pKa = 11.84 EE316 pKa = 3.39 KK317 pKa = 10.1 GTEE320 pKa = 3.8 EE321 pKa = 3.67 RR322 pKa = 11.84 RR323 pKa = 11.84 ARR325 pKa = 11.84 PPPAPSPPFARR336 pKa = 11.84 RR337 pKa = 11.84 RR338 pKa = 11.84 SLGASSLRR346 pKa = 11.84 LPVLARR352 pKa = 11.84 GKK354 pKa = 9.78 RR355 pKa = 11.84 EE356 pKa = 3.47 PRR358 pKa = 11.84 RR359 pKa = 11.84 GVRR362 pKa = 11.84 CSRR365 pKa = 11.84 ARR367 pKa = 11.84 NDD369 pKa = 2.92 AAARR373 pKa = 11.84 RR374 pKa = 11.84 TKK376 pKa = 10.54 KK377 pKa = 10.5 KK378 pKa = 10.15 EE379 pKa = 3.71 EE380 pKa = 4.02 KK381 pKa = 10.61
Molecular weight: 42.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.366
IPC2_protein 10.789
IPC_protein 12.398
Toseland 12.559
ProMoST 13.056
Dawson 12.559
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.106
Grimsley 12.603
Solomon 13.056
Lehninger 12.969
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 11.813
IPC_peptide 13.071
IPC2_peptide 12.062
IPC2.peptide.svr19 9.174
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
172
0
172
59641
53
1941
346.8
38.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.92 ± 0.41
2.113 ± 0.088
6.195 ± 0.133
5.761 ± 0.125
4.604 ± 0.148
5.795 ± 0.245
1.856 ± 0.078
4.489 ± 0.189
3.711 ± 0.191
9.74 ± 0.199
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.88 ± 0.066
3.944 ± 0.154
5.508 ± 0.281
2.264 ± 0.102
8.56 ± 0.291
7.567 ± 0.216
4.755 ± 0.112
7.741 ± 0.161
0.676 ± 0.036
3.923 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here