Petromyzon marinus (Sea lamprey)
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11407 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S4RPW3|S4RPW3_PETMA Isoform of S4RPV9 BRF1 RNA polymerase III transcription initiation factor subunit OS=Petromyzon marinus OX=7757 PE=4 SV=1
TT1 pKa = 7.43 ALLGNDD7 pKa = 3.15 NVYY10 pKa = 10.78 EE11 pKa = 4.16 LNLDD15 pKa = 3.86 YY16 pKa = 11.39 NQLQTLSAGVFDD28 pKa = 6.15 DD29 pKa = 3.79 LTEE32 pKa = 4.7 LGTLGLANNQLASLPLGVFADD53 pKa = 4.79 LAPTDD58 pKa = 4.62 DD59 pKa = 5.16 LYY61 pKa = 12.08 LNINQLKK68 pKa = 9.37 SLPSGVFDD76 pKa = 4.87 SLTKK80 pKa = 9.99 LTILQLDD87 pKa = 4.13 YY88 pKa = 11.43 NQLQSVPHH96 pKa = 6.1 GAFDD100 pKa = 5.0 RR101 pKa = 11.84 LTNLQTMQLHH111 pKa = 6.26 SDD113 pKa = 3.84 VLQSVPNGAFNALTKK128 pKa = 10.88 LEE130 pKa = 4.56 TITLNVNVWDD140 pKa = 4.88 CSSCDD145 pKa = 3.21 ILYY148 pKa = 10.69 LSEE151 pKa = 4.02
Molecular weight: 16.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.745
IPC_protein 3.732
Toseland 3.503
ProMoST 3.884
Dawson 3.745
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.139
Thurlkill 3.579
EMBOSS 3.732
Sillero 3.859
Patrickios 0.54
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|S4RWB8|S4RWB8_PETMA RNA-binding motif protein 42 OS=Petromyzon marinus OX=7757 PE=3 SV=1
PP1 pKa = 7.3 SPLDD5 pKa = 3.43 SSEE8 pKa = 3.94 AATAQRR14 pKa = 11.84 AKK16 pKa = 10.45 FADD19 pKa = 3.92 SSSSTLFTSMAALNHH34 pKa = 6.51 YY35 pKa = 7.63 DD36 pKa = 3.53 THH38 pKa = 7.6 CNGYY42 pKa = 8.44 HH43 pKa = 6.46 CKK45 pKa = 10.2 HH46 pKa = 5.26 VVRR49 pKa = 11.84 LISRR53 pKa = 11.84 HH54 pKa = 5.18 LIARR58 pKa = 11.84 KK59 pKa = 9.57 VNVVRR64 pKa = 11.84 LIIRR68 pKa = 11.84 HH69 pKa = 5.17 LNARR73 pKa = 11.84 KK74 pKa = 9.83 VNVVRR79 pKa = 11.84 LIIRR83 pKa = 11.84 HH84 pKa = 5.91 LIARR88 pKa = 11.84 KK89 pKa = 9.27 VNVVRR94 pKa = 11.84 LISRR98 pKa = 11.84 HH99 pKa = 5.18 LIARR103 pKa = 11.84 KK104 pKa = 9.57 VNVVRR109 pKa = 11.84 LIIRR113 pKa = 11.84 HH114 pKa = 5.91 LIARR118 pKa = 11.84 KK119 pKa = 9.27 VNVVRR124 pKa = 11.84 LIIRR128 pKa = 11.84 HH129 pKa = 5.91 LIARR133 pKa = 11.84 KK134 pKa = 9.37 VNMVRR139 pKa = 11.84 LISRR143 pKa = 11.84 HH144 pKa = 5.17 LIARR148 pKa = 11.84 KK149 pKa = 9.57 VNVVRR154 pKa = 11.84 LIIRR158 pKa = 11.84 HH159 pKa = 5.17 LNARR163 pKa = 11.84 KK164 pKa = 9.83 VNVVRR169 pKa = 11.84 LIIRR173 pKa = 11.84 HH174 pKa = 5.24 LNARR178 pKa = 11.84 KK179 pKa = 9.21 HH180 pKa = 5.65 LIARR184 pKa = 11.84 KK185 pKa = 8.92 VNVVRR190 pKa = 11.84 LIIRR194 pKa = 11.84 HH195 pKa = 5.17 LNARR199 pKa = 11.84 KK200 pKa = 9.83 VNVVRR205 pKa = 11.84 LIIRR209 pKa = 11.84 HH210 pKa = 5.91 LIARR214 pKa = 11.84 KK215 pKa = 9.27 VNVVRR220 pKa = 11.84 LIIRR224 pKa = 11.84 HH225 pKa = 5.91 LIARR229 pKa = 11.84 KK230 pKa = 9.27 VNVVRR235 pKa = 11.84 LIIRR239 pKa = 11.84 HH240 pKa = 5.55 LNARR244 pKa = 11.84 HH245 pKa = 5.07 LNARR249 pKa = 11.84 KK250 pKa = 10.39 VNVHH254 pKa = 6.53 LIARR258 pKa = 11.84 KK259 pKa = 9.14 VNVVRR264 pKa = 11.84 LIIRR268 pKa = 11.84 HH269 pKa = 5.17 LNARR273 pKa = 11.84 KK274 pKa = 9.83 VNVVRR279 pKa = 11.84 LIILHH284 pKa = 6.41 LIARR288 pKa = 11.84 KK289 pKa = 9.81 VNVVRR294 pKa = 11.84 LIIRR298 pKa = 11.84 HH299 pKa = 5.91 LIARR303 pKa = 11.84 KK304 pKa = 9.27 VNVVRR309 pKa = 11.84 LIIRR313 pKa = 11.84 HH314 pKa = 5.17 LNARR318 pKa = 11.84 KK319 pKa = 10.12 VNVVHH324 pKa = 7.05 LIIRR328 pKa = 11.84 HH329 pKa = 5.63 LIARR333 pKa = 11.84 KK334 pKa = 9.27 VNVVRR339 pKa = 11.84 LIIQHH344 pKa = 6.61 LIARR348 pKa = 11.84 KK349 pKa = 8.99 VNVVRR354 pKa = 11.84 LIIRR358 pKa = 11.84 HH359 pKa = 5.17 LNARR363 pKa = 11.84 KK364 pKa = 9.83 VNVVRR369 pKa = 11.84 LIIRR373 pKa = 11.84 HH374 pKa = 5.91 LIARR378 pKa = 11.84 KK379 pKa = 9.27 VNVVRR384 pKa = 11.84 LIIRR388 pKa = 11.84 HH389 pKa = 5.91 LIARR393 pKa = 11.84 KK394 pKa = 9.27 VNVVRR399 pKa = 11.84 LIIRR403 pKa = 11.84 HH404 pKa = 5.91 LIARR408 pKa = 11.84 KK409 pKa = 9.27 VNVVRR414 pKa = 11.84 LIIRR418 pKa = 11.84 HH419 pKa = 5.17 LNARR423 pKa = 11.84 KK424 pKa = 9.83 VNVVRR429 pKa = 11.84 LIIRR433 pKa = 11.84 HH434 pKa = 5.29 LNARR438 pKa = 11.84 KK439 pKa = 9.99 ANVVRR444 pKa = 11.84 LIIRR448 pKa = 11.84 HH449 pKa = 5.17 LNARR453 pKa = 11.84 KK454 pKa = 9.83 VNVVRR459 pKa = 11.84 LIIRR463 pKa = 11.84 HH464 pKa = 5.91 LIARR468 pKa = 11.84 KK469 pKa = 9.27 VNVVRR474 pKa = 11.84 LIIRR478 pKa = 11.84 HH479 pKa = 5.91 LIARR483 pKa = 11.84 KK484 pKa = 9.27 VNVVRR489 pKa = 11.84 LIIRR493 pKa = 11.84 HH494 pKa = 5.91 LIARR498 pKa = 11.84 KK499 pKa = 9.27 VNVVRR504 pKa = 11.84 LIIRR508 pKa = 11.84 HH509 pKa = 5.17 LNARR513 pKa = 11.84 KK514 pKa = 10.36 VNVV517 pKa = 3.29
Molecular weight: 60.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.272
IPC_protein 12.808
Toseland 12.969
ProMoST 13.466
Dawson 12.969
Bjellqvist 12.969
Wikipedia 13.437
Rodwell 12.618
Grimsley 13.013
Solomon 13.466
Lehninger 13.364
Nozaki 12.969
DTASelect 12.969
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.31
IPC_peptide 13.466
IPC2_peptide 12.457
IPC2.peptide.svr19 9.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10375
1032
11407
4607059
9
18900
403.9
44.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.042 ± 0.029
2.376 ± 0.025
5.078 ± 0.019
6.47 ± 0.032
3.634 ± 0.018
7.049 ± 0.034
2.772 ± 0.014
3.971 ± 0.02
4.81 ± 0.031
9.97 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.235 ± 0.012
3.419 ± 0.019
5.557 ± 0.032
4.355 ± 0.025
6.482 ± 0.025
7.628 ± 0.026
5.287 ± 0.024
6.914 ± 0.021
1.28 ± 0.01
2.666 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here