Porphyromonas crevioricanis
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1804 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2X4PZM0|A0A2X4PZM0_9PORP Phosphopantetheine adenylyltransferase OS=Porphyromonas crevioricanis OX=393921 GN=coaD PE=3 SV=1
MM1 pKa = 7.83 RR2 pKa = 11.84 KK3 pKa = 9.76 YY4 pKa = 10.46 ICTLCDD10 pKa = 3.08 WVYY13 pKa = 11.37 DD14 pKa = 4.13 PAVGDD19 pKa = 3.99 PDD21 pKa = 4.71 GGIAPGTAFEE31 pKa = 6.0 DD32 pKa = 4.51 IPDD35 pKa = 3.89 DD36 pKa = 3.81 WSCPVCGATKK46 pKa = 10.71 DD47 pKa = 3.54 DD48 pKa = 4.3 FEE50 pKa = 4.5 VHH52 pKa = 6.47 KK53 pKa = 11.12 GG54 pKa = 3.39
Molecular weight: 5.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.961
IPC_protein 3.897
Toseland 3.668
ProMoST 4.101
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.91
Rodwell 3.719
Grimsley 3.592
Solomon 3.897
Lehninger 3.846
Nozaki 4.05
DTASelect 4.329
Thurlkill 3.757
EMBOSS 3.91
Sillero 4.024
Patrickios 0.782
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.905
Protein with the highest isoelectric point:
>tr|A0A2X4PJQ9|A0A2X4PJQ9_9PORP Endolytic murein transglycosylase OS=Porphyromonas crevioricanis OX=393921 GN=yceG PE=3 SV=1
MM1 pKa = 6.62 QAMRR5 pKa = 11.84 VRR7 pKa = 11.84 LQEE10 pKa = 4.22 RR11 pKa = 11.84 VDD13 pKa = 3.67 KK14 pKa = 11.29 KK15 pKa = 11.35 KK16 pKa = 8.26 MTQARR21 pKa = 11.84 ILIGYY26 pKa = 7.87 NRR28 pKa = 11.84 SKK30 pKa = 11.02 FEE32 pKa = 4.72 CNSAHH37 pKa = 6.07 NRR39 pKa = 11.84 IVLSACSQLIASVYY53 pKa = 8.33 NWVVPSVCNQLIASVYY69 pKa = 8.68 NRR71 pKa = 11.84 VVPSVCNQIIPFPRR85 pKa = 11.84 RR86 pKa = 3.09
Molecular weight: 9.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.692
IPC_protein 10.452
Toseland 10.496
ProMoST 10.511
Dawson 10.643
Bjellqvist 10.379
Wikipedia 10.847
Rodwell 10.862
Grimsley 10.701
Solomon 10.73
Lehninger 10.687
Nozaki 10.54
DTASelect 10.35
Thurlkill 10.526
EMBOSS 10.891
Sillero 10.584
Patrickios 10.643
IPC_peptide 10.73
IPC2_peptide 9.721
IPC2.peptide.svr19 8.49
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1804
0
1804
611482
30
2456
339.0
38.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.462 ± 0.059
1.229 ± 0.021
5.374 ± 0.038
6.844 ± 0.061
4.186 ± 0.037
6.792 ± 0.042
2.044 ± 0.024
6.716 ± 0.046
6.019 ± 0.053
10.134 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.026
4.027 ± 0.042
4.11 ± 0.037
3.682 ± 0.034
5.569 ± 0.043
6.881 ± 0.051
5.041 ± 0.042
6.366 ± 0.048
1.022 ± 0.023
3.948 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here