Yongsan tombus-like virus 1
Average proteome isoelectric point is 8.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385FQG9|A0A385FQG9_9TOMB Uncharacterized protein OS=Yongsan tombus-like virus 1 OX=2315813 PE=4 SV=1
MM1 pKa = 7.41 SLNDD5 pKa = 3.46 KK6 pKa = 9.87 VLPGSCLSAGACDD19 pKa = 4.24 HH20 pKa = 5.37 VCKK23 pKa = 10.49 RR24 pKa = 11.84 YY25 pKa = 6.35 TQQMFTIQGFTSEE38 pKa = 4.12 VWTHH42 pKa = 6.14 GSCVCNEE49 pKa = 3.94 VIALKK54 pKa = 10.37 NRR56 pKa = 11.84 HH57 pKa = 5.21 QLCDD61 pKa = 3.4 GSAYY65 pKa = 10.81 NSTVDD70 pKa = 4.02 LRR72 pKa = 11.84 RR73 pKa = 11.84 PLRR76 pKa = 11.84 KK77 pKa = 9.78 LITNLEE83 pKa = 4.29 PCSEE87 pKa = 4.25 QTVIAHH93 pKa = 7.28 ADD95 pKa = 3.5 GSRR98 pKa = 11.84 KK99 pKa = 9.7 KK100 pKa = 10.46 LLQRR104 pKa = 11.84 AADD107 pKa = 3.68 SLSTHH112 pKa = 5.62 GLEE115 pKa = 6.11 HH116 pKa = 6.74 KK117 pKa = 10.28 DD118 pKa = 2.94 AWVKK122 pKa = 10.38 MFLKK126 pKa = 10.25 DD127 pKa = 4.24 DD128 pKa = 4.11 KK129 pKa = 11.43 YY130 pKa = 10.51 HH131 pKa = 5.34 TPEE134 pKa = 4.02 YY135 pKa = 9.99 KK136 pKa = 10.36 APRR139 pKa = 11.84 CIQYY143 pKa = 10.18 RR144 pKa = 11.84 NKK146 pKa = 10.62 RR147 pKa = 11.84 YY148 pKa = 9.98 GLRR151 pKa = 11.84 LATFLHH157 pKa = 6.98 PIEE160 pKa = 4.32 QRR162 pKa = 11.84 VITLKK167 pKa = 11.09 HH168 pKa = 5.94 NGTHH172 pKa = 5.58 VFAKK176 pKa = 9.07 GRR178 pKa = 11.84 NMKK181 pKa = 9.93 QRR183 pKa = 11.84 GRR185 pKa = 11.84 DD186 pKa = 3.19 IAAKK190 pKa = 10.39 LLPNWVAVSMDD201 pKa = 3.08 HH202 pKa = 7.32 SKK204 pKa = 10.78 FDD206 pKa = 3.49 SHH208 pKa = 8.04 VNEE211 pKa = 3.89 HH212 pKa = 6.67 LLRR215 pKa = 11.84 LEE217 pKa = 3.5 HH218 pKa = 6.94 WYY220 pKa = 10.98 YY221 pKa = 10.11 NQCINDD227 pKa = 4.41 PEE229 pKa = 4.66 LKK231 pKa = 9.85 WLLEE235 pKa = 3.97 CQLRR239 pKa = 11.84 NKK241 pKa = 10.33 GFTKK245 pKa = 10.75 NNTRR249 pKa = 11.84 YY250 pKa = 5.23 TTRR253 pKa = 11.84 ATRR256 pKa = 11.84 MSGDD260 pKa = 3.33 QNTGLGNCIINYY272 pKa = 10.34 AMTKK276 pKa = 10.88 AMLDD280 pKa = 3.56 HH281 pKa = 7.29 LDD283 pKa = 3.84 IPHH286 pKa = 6.82 NLYY289 pKa = 10.06 IDD291 pKa = 3.99 GDD293 pKa = 4.02 DD294 pKa = 4.51 FIVFVHH300 pKa = 6.87 RR301 pKa = 11.84 KK302 pKa = 8.23 HH303 pKa = 6.37 KK304 pKa = 10.45 HH305 pKa = 5.78 LIDD308 pKa = 4.4 PNWYY312 pKa = 9.1 KK313 pKa = 10.65 QFGMKK318 pKa = 7.62 TTTDD322 pKa = 3.26 QITDD326 pKa = 3.62 VLEE329 pKa = 5.48 HH330 pKa = 7.39 IDD332 pKa = 3.8 FCQCRR337 pKa = 11.84 PVYY340 pKa = 10.9 DD341 pKa = 3.96 GDD343 pKa = 4.0 SYY345 pKa = 11.56 TLVRR349 pKa = 11.84 NPARR353 pKa = 11.84 LLARR357 pKa = 11.84 LPWLVGPINGRR368 pKa = 11.84 SPWNIVASAAQCEE381 pKa = 4.11 ISLGYY386 pKa = 10.06 GLPIGQYY393 pKa = 8.61 VGHH396 pKa = 6.44 NVFLYY401 pKa = 10.7 ASNKK405 pKa = 9.28 GGRR408 pKa = 11.84 FKK410 pKa = 11.27 SNCVAEE416 pKa = 4.66 WRR418 pKa = 11.84 HH419 pKa = 5.18 KK420 pKa = 8.27 MEE422 pKa = 4.7 RR423 pKa = 11.84 MKK425 pKa = 10.84 AGNLKK430 pKa = 10.22 PVDD433 pKa = 3.77 CAPGVRR439 pKa = 11.84 ASYY442 pKa = 9.63 EE443 pKa = 4.13 RR444 pKa = 11.84 AWGISIPQQYY454 pKa = 9.78 IIEE457 pKa = 4.62 HH458 pKa = 5.85 ATIAPPFEE466 pKa = 4.69 EE467 pKa = 4.98 EE468 pKa = 3.76 WDD470 pKa = 3.5 HH471 pKa = 6.74 TPFDD475 pKa = 4.04 RR476 pKa = 11.84 KK477 pKa = 8.86 TYY479 pKa = 10.53
Molecular weight: 55.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.205
IPC2_protein 8.229
IPC_protein 8.053
Toseland 7.966
ProMoST 8.682
Dawson 8.843
Bjellqvist 9.063
Wikipedia 8.843
Rodwell 8.916
Grimsley 7.995
Solomon 8.916
Lehninger 8.902
Nozaki 9.253
DTASelect 8.829
Thurlkill 8.902
EMBOSS 9.019
Sillero 9.18
Patrickios 4.329
IPC_peptide 8.902
IPC2_peptide 8.024
IPC2.peptide.svr19 8.041
Protein with the highest isoelectric point:
>tr|A0A385FQH1|A0A385FQH1_9TOMB Uncharacterized protein OS=Yongsan tombus-like virus 1 OX=2315813 PE=4 SV=1
MM1 pKa = 7.93 RR2 pKa = 11.84 ALQVEE7 pKa = 4.48 LQHH10 pKa = 6.86 LRR12 pKa = 11.84 RR13 pKa = 11.84 ANSALQARR21 pKa = 11.84 VRR23 pKa = 11.84 DD24 pKa = 3.88 LEE26 pKa = 4.07 GLITRR31 pKa = 11.84 NTSAPLGGSGPITTIPRR48 pKa = 11.84 GSRR51 pKa = 11.84 RR52 pKa = 11.84 STLVRR57 pKa = 11.84 ASGVASTPNLNSSPEE72 pKa = 4.0 PRR74 pKa = 11.84 SQRR77 pKa = 11.84 PVPHH81 pKa = 6.71 VNTTPPVSRR90 pKa = 11.84 RR91 pKa = 11.84 QSSTSPRR98 pKa = 11.84 VGTPSRR104 pKa = 11.84 PQSVTALTRR113 pKa = 11.84 PVTPASAGQAAEE125 pKa = 4.16 HH126 pKa = 6.13 STVSTQLSSGPALSPAPEE144 pKa = 4.11 TTASKK149 pKa = 9.68 TVVSHH154 pKa = 6.92 PYY156 pKa = 10.19 AGAILEE162 pKa = 4.09 ATRR165 pKa = 11.84 AKK167 pKa = 10.96 GEE169 pKa = 4.07 LAHH172 pKa = 7.13 DD173 pKa = 3.76 EE174 pKa = 4.39 RR175 pKa = 11.84 PVYY178 pKa = 10.39 VADD181 pKa = 3.72 HH182 pKa = 6.78 AIDD185 pKa = 3.56 RR186 pKa = 11.84 SLRR189 pKa = 11.84 EE190 pKa = 3.84 SVGITRR196 pKa = 11.84 RR197 pKa = 11.84 AVNKK201 pKa = 6.42 MTAVDD206 pKa = 3.72 EE207 pKa = 4.41 EE208 pKa = 4.72 EE209 pKa = 4.28 PKK211 pKa = 11.21 GMWRR215 pKa = 11.84 YY216 pKa = 8.98 LPKK219 pKa = 10.26 VRR221 pKa = 11.84 SPALRR226 pKa = 11.84 ADD228 pKa = 3.42 KK229 pKa = 11.05 YY230 pKa = 11.73 LLFFLLNKK238 pKa = 9.92 FAFIEE243 pKa = 4.19 RR244 pKa = 11.84 SPDD247 pKa = 2.62 TMRR250 pKa = 11.84 RR251 pKa = 11.84 MWEE254 pKa = 3.89 SLNQVMRR261 pKa = 11.84 DD262 pKa = 2.94 FDD264 pKa = 3.9 TRR266 pKa = 11.84 NNTIEE271 pKa = 4.27 EE272 pKa = 4.35 IYY274 pKa = 10.41 LLKK277 pKa = 10.5 HH278 pKa = 4.54 QAVRR282 pKa = 11.84 AAMVPPAEE290 pKa = 4.27 EE291 pKa = 3.69 LRR293 pKa = 11.84 TRR295 pKa = 11.84 KK296 pKa = 9.48 LLQEE300 pKa = 4.39 APNLDD305 pKa = 3.2 KK306 pKa = 10.87 HH307 pKa = 7.15 AKK309 pKa = 7.03 FTKK312 pKa = 10.08 DD313 pKa = 2.53 GHH315 pKa = 6.69 LGHH318 pKa = 7.07 KK319 pKa = 10.33 LEE321 pKa = 5.29 ASLSTIGNLFQPTKK335 pKa = 10.72 SLPTKK340 pKa = 10.28 KK341 pKa = 10.5
Molecular weight: 37.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.619
IPC_protein 10.438
Toseland 10.818
ProMoST 10.555
Dawson 10.877
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.067
Grimsley 10.921
Solomon 11.038
Lehninger 11.008
Nozaki 10.789
DTASelect 10.599
Thurlkill 10.804
EMBOSS 11.228
Sillero 10.818
Patrickios 10.774
IPC_peptide 11.052
IPC2_peptide 9.297
IPC2.peptide.svr19 8.851
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1228
341
479
409.3
45.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.062 ± 0.909
1.547 ± 0.927
3.746 ± 1.058
4.56 ± 0.488
2.85 ± 0.3
5.537 ± 0.35
3.094 ± 0.998
4.642 ± 0.66
4.886 ± 0.818
9.365 ± 0.295
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.954 ± 0.2
4.479 ± 0.594
6.84 ± 1.134
4.56 ± 0.611
7.166 ± 1.061
7.573 ± 1.383
8.143 ± 1.288
6.596 ± 0.756
1.466 ± 0.453
2.932 ± 0.705
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here