Staphylococcus phage vB_SpsS_QT1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fibralongavirus; Staphylococcus virus QT1

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P6R242|A0A4P6R242_9CAUD DUF2951 domain-containing protein OS=Staphylococcus phage vB_SpsS_QT1 OX=2510452 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.61YY3 pKa = 10.24IITTAMILYY12 pKa = 9.7IAYY15 pKa = 9.48QYY17 pKa = 10.17YY18 pKa = 9.99IKK20 pKa = 10.06RR21 pKa = 11.84TANDD25 pKa = 3.02EE26 pKa = 3.67VDD28 pKa = 3.91AFNEE32 pKa = 3.86YY33 pKa = 11.29DD34 pKa = 5.33HH35 pKa = 7.39IDD37 pKa = 3.83LNNVRR42 pKa = 11.84AEE44 pKa = 4.0VSEE47 pKa = 4.13

Molecular weight: 5.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P6QX91|A0A4P6QX91_9CAUD DNA polymerase A with ribonuclease H-like domain OS=Staphylococcus phage vB_SpsS_QT1 OX=2510452 PE=3 SV=1
MM1 pKa = 7.17SASHH5 pKa = 7.21RR6 pKa = 11.84DD7 pKa = 3.31LSNVKK12 pKa = 9.54RR13 pKa = 11.84AKK15 pKa = 10.26YY16 pKa = 9.99KK17 pKa = 10.89YY18 pKa = 8.07NTAGKK23 pKa = 8.07TPTEE27 pKa = 4.0LQRR30 pKa = 11.84EE31 pKa = 4.19LRR33 pKa = 11.84KK34 pKa = 10.21RR35 pKa = 11.84GVKK38 pKa = 10.33GFVVNVNHH46 pKa = 6.25NRR48 pKa = 11.84VTMLVDD54 pKa = 3.12RR55 pKa = 11.84RR56 pKa = 11.84DD57 pKa = 3.27VKK59 pKa = 10.88RR60 pKa = 11.84NKK62 pKa = 9.93EE63 pKa = 4.02CMRR66 pKa = 4.66

Molecular weight: 7.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

13549

43

2031

225.8

25.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.207 ± 0.547

0.509 ± 0.113

6.369 ± 0.541

7.314 ± 0.547

4.318 ± 0.26

6.03 ± 0.513

1.779 ± 0.158

6.871 ± 0.265

9.167 ± 0.324

8.163 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.738 ± 0.203

6.111 ± 0.237

2.657 ± 0.193

4.008 ± 0.184

4.347 ± 0.185

6.03 ± 0.463

5.912 ± 0.274

6.023 ± 0.197

1.336 ± 0.104

4.111 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski