Amino acid dipepetide frequency for Staphylococcus phage vB_SpsS_QT1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.617AlaAla: 3.617 ± 1.09
0.074AlaCys: 0.074 ± 0.092
3.617AlaAsp: 3.617 ± 0.613
4.724AlaGlu: 4.724 ± 0.508
2.805AlaPhe: 2.805 ± 0.413
3.617AlaGly: 3.617 ± 0.663
0.96AlaHis: 0.96 ± 0.298
4.576AlaIle: 4.576 ± 0.617
4.355AlaLys: 4.355 ± 0.627
6.717AlaLeu: 6.717 ± 0.75
1.845AlaMet: 1.845 ± 0.421
3.174AlaAsn: 3.174 ± 0.642
1.624AlaPro: 1.624 ± 0.326
2.805AlaGln: 2.805 ± 0.684
2.952AlaArg: 2.952 ± 0.436
3.691AlaSer: 3.691 ± 0.6
4.133AlaThr: 4.133 ± 0.705
2.879AlaVal: 2.879 ± 0.583
0.738AlaTrp: 0.738 ± 0.2
3.174AlaTyr: 3.174 ± 0.447
0.0AlaXaa: 0.0 ± 0.0
Cys
0.221CysAla: 0.221 ± 0.129
0.074CysCys: 0.074 ± 0.092
0.221CysAsp: 0.221 ± 0.132
0.148CysGlu: 0.148 ± 0.101
0.295CysPhe: 0.295 ± 0.145
0.517CysGly: 0.517 ± 0.228
0.074CysHis: 0.074 ± 0.068
0.369CysIle: 0.369 ± 0.172
0.664CysLys: 0.664 ± 0.255
0.59CysLeu: 0.59 ± 0.204
0.148CysMet: 0.148 ± 0.131
0.148CysAsn: 0.148 ± 0.095
0.148CysPro: 0.148 ± 0.091
0.074CysGln: 0.074 ± 0.076
0.295CysArg: 0.295 ± 0.15
0.295CysSer: 0.295 ± 0.137
0.148CysThr: 0.148 ± 0.089
0.517CysVal: 0.517 ± 0.175
0.074CysTrp: 0.074 ± 0.071
0.074CysTyr: 0.074 ± 0.079
0.0CysXaa: 0.0 ± 0.0
Asp
3.395AspAla: 3.395 ± 0.522
0.074AspCys: 0.074 ± 0.069
4.429AspAsp: 4.429 ± 0.817
6.274AspGlu: 6.274 ± 0.762
2.952AspPhe: 2.952 ± 0.496
4.06AspGly: 4.06 ± 0.763
0.886AspHis: 0.886 ± 0.246
4.133AspIle: 4.133 ± 0.716
6.569AspLys: 6.569 ± 0.709
4.945AspLeu: 4.945 ± 0.616
2.067AspMet: 2.067 ± 0.555
3.395AspAsn: 3.395 ± 0.794
1.771AspPro: 1.771 ± 0.344
1.255AspGln: 1.255 ± 0.357
2.731AspArg: 2.731 ± 0.574
2.583AspSer: 2.583 ± 0.503
3.838AspThr: 3.838 ± 0.542
4.355AspVal: 4.355 ± 0.549
1.033AspTrp: 1.033 ± 0.243
2.952AspTyr: 2.952 ± 0.566
0.0AspXaa: 0.0 ± 0.0
Glu
5.683GluAla: 5.683 ± 0.502
0.664GluCys: 0.664 ± 0.271
3.026GluAsp: 3.026 ± 0.559
6.569GluGlu: 6.569 ± 1.148
2.952GluPhe: 2.952 ± 0.539
3.026GluGly: 3.026 ± 0.526
1.181GluHis: 1.181 ± 0.389
5.093GluIle: 5.093 ± 0.453
5.831GluLys: 5.831 ± 0.871
7.086GluLeu: 7.086 ± 0.869
2.141GluMet: 2.141 ± 0.352
4.65GluAsn: 4.65 ± 0.829
1.55GluPro: 1.55 ± 0.379
3.1GluGln: 3.1 ± 0.526
3.469GluArg: 3.469 ± 0.64
3.912GluSer: 3.912 ± 0.576
3.691GluThr: 3.691 ± 0.398
5.019GluVal: 5.019 ± 1.027
1.402GluTrp: 1.402 ± 0.314
3.1GluTyr: 3.1 ± 0.692
0.0GluXaa: 0.0 ± 0.0
Phe
2.362PheAla: 2.362 ± 0.451
0.369PheCys: 0.369 ± 0.135
3.248PheAsp: 3.248 ± 0.584
3.026PheGlu: 3.026 ± 0.725
1.771PhePhe: 1.771 ± 0.365
2.583PheGly: 2.583 ± 0.38
0.295PheHis: 0.295 ± 0.23
3.395PheIle: 3.395 ± 0.522
4.503PheLys: 4.503 ± 0.646
1.993PheLeu: 1.993 ± 0.483
1.255PheMet: 1.255 ± 0.487
3.838PheAsn: 3.838 ± 0.688
0.812PhePro: 0.812 ± 0.183
1.329PheGln: 1.329 ± 0.43
1.181PheArg: 1.181 ± 0.348
3.469PheSer: 3.469 ± 0.382
2.657PheThr: 2.657 ± 0.489
2.436PheVal: 2.436 ± 0.499
0.664PheTrp: 0.664 ± 0.212
1.993PheTyr: 1.993 ± 0.4
0.0PheXaa: 0.0 ± 0.0
Gly
4.503GlyAla: 4.503 ± 0.905
0.369GlyCys: 0.369 ± 0.197
3.764GlyAsp: 3.764 ± 0.593
2.805GlyGlu: 2.805 ± 0.462
2.879GlyPhe: 2.879 ± 0.375
5.314GlyGly: 5.314 ± 1.189
1.107GlyHis: 1.107 ± 0.264
3.912GlyIle: 3.912 ± 0.651
5.979GlyLys: 5.979 ± 0.637
5.831GlyLeu: 5.831 ± 0.787
0.96GlyMet: 0.96 ± 0.295
2.879GlyAsn: 2.879 ± 0.547
1.107GlyPro: 1.107 ± 0.273
1.919GlyGln: 1.919 ± 0.412
2.288GlyArg: 2.288 ± 0.361
3.1GlySer: 3.1 ± 0.628
4.355GlyThr: 4.355 ± 0.482
4.503GlyVal: 4.503 ± 0.682
0.812GlyTrp: 0.812 ± 0.293
1.919GlyTyr: 1.919 ± 0.317
0.0GlyXaa: 0.0 ± 0.0
His
1.033HisAla: 1.033 ± 0.328
0.295HisCys: 0.295 ± 0.125
1.033HisAsp: 1.033 ± 0.318
1.329HisGlu: 1.329 ± 0.321
0.738HisPhe: 0.738 ± 0.279
1.033HisGly: 1.033 ± 0.239
0.517HisHis: 0.517 ± 0.249
1.255HisIle: 1.255 ± 0.309
0.886HisLys: 0.886 ± 0.228
1.624HisLeu: 1.624 ± 0.355
0.443HisMet: 0.443 ± 0.177
1.329HisAsn: 1.329 ± 0.45
0.59HisPro: 0.59 ± 0.2
0.59HisGln: 0.59 ± 0.221
0.812HisArg: 0.812 ± 0.258
1.181HisSer: 1.181 ± 0.364
1.107HisThr: 1.107 ± 0.248
0.812HisVal: 0.812 ± 0.271
0.221HisTrp: 0.221 ± 0.135
0.96HisTyr: 0.96 ± 0.263
0.0HisXaa: 0.0 ± 0.0
Ile
3.986IleAla: 3.986 ± 0.726
0.148IleCys: 0.148 ± 0.114
5.388IleAsp: 5.388 ± 0.824
5.019IleGlu: 5.019 ± 0.873
2.731IlePhe: 2.731 ± 0.527
2.141IleGly: 2.141 ± 0.359
1.329IleHis: 1.329 ± 0.352
4.429IleIle: 4.429 ± 0.514
7.455IleLys: 7.455 ± 0.789
4.429IleLeu: 4.429 ± 0.664
1.402IleMet: 1.402 ± 0.394
5.757IleAsn: 5.757 ± 0.689
2.067IlePro: 2.067 ± 0.363
2.141IleGln: 2.141 ± 0.37
2.583IleArg: 2.583 ± 0.415
4.945IleSer: 4.945 ± 0.552
4.355IleThr: 4.355 ± 0.669
5.093IleVal: 5.093 ± 0.706
0.443IleTrp: 0.443 ± 0.168
2.879IleTyr: 2.879 ± 0.449
0.0IleXaa: 0.0 ± 0.0
Lys
5.683LysAla: 5.683 ± 0.695
0.369LysCys: 0.369 ± 0.198
6.2LysAsp: 6.2 ± 0.587
5.683LysGlu: 5.683 ± 0.839
3.543LysPhe: 3.543 ± 0.498
6.569LysGly: 6.569 ± 1.063
2.214LysHis: 2.214 ± 0.393
6.126LysIle: 6.126 ± 0.507
7.603LysLys: 7.603 ± 0.971
7.455LysLeu: 7.455 ± 0.631
2.879LysMet: 2.879 ± 0.477
4.503LysAsn: 4.503 ± 0.612
2.583LysPro: 2.583 ± 0.465
5.314LysGln: 5.314 ± 0.532
4.724LysArg: 4.724 ± 0.613
4.06LysSer: 4.06 ± 0.914
5.167LysThr: 5.167 ± 0.622
5.241LysVal: 5.241 ± 0.557
1.771LysTrp: 1.771 ± 0.532
3.986LysTyr: 3.986 ± 0.738
0.0LysXaa: 0.0 ± 0.0
Leu
4.945LeuAla: 4.945 ± 0.657
0.664LeuCys: 0.664 ± 0.218
5.536LeuAsp: 5.536 ± 0.819
6.274LeuGlu: 6.274 ± 0.647
3.026LeuPhe: 3.026 ± 0.5
3.764LeuGly: 3.764 ± 0.543
1.033LeuHis: 1.033 ± 0.289
5.019LeuIle: 5.019 ± 0.495
9.374LeuLys: 9.374 ± 0.756
6.495LeuLeu: 6.495 ± 0.561
1.993LeuMet: 1.993 ± 0.408
5.536LeuAsn: 5.536 ± 0.678
1.919LeuPro: 1.919 ± 0.346
3.838LeuGln: 3.838 ± 0.661
3.691LeuArg: 3.691 ± 0.423
5.683LeuSer: 5.683 ± 0.753
5.093LeuThr: 5.093 ± 0.443
4.133LeuVal: 4.133 ± 0.49
0.96LeuTrp: 0.96 ± 0.259
2.657LeuTyr: 2.657 ± 0.479
0.0LeuXaa: 0.0 ± 0.0
Met
1.255MetAla: 1.255 ± 0.252
0.148MetCys: 0.148 ± 0.089
1.181MetAsp: 1.181 ± 0.322
1.845MetGlu: 1.845 ± 0.364
1.181MetPhe: 1.181 ± 0.338
1.329MetGly: 1.329 ± 0.406
0.664MetHis: 0.664 ± 0.244
1.624MetIle: 1.624 ± 0.377
2.805MetLys: 2.805 ± 0.591
1.55MetLeu: 1.55 ± 0.349
0.812MetMet: 0.812 ± 0.251
1.329MetAsn: 1.329 ± 0.307
1.476MetPro: 1.476 ± 0.332
1.771MetGln: 1.771 ± 0.358
1.402MetArg: 1.402 ± 0.326
2.879MetSer: 2.879 ± 0.735
1.771MetThr: 1.771 ± 0.27
1.181MetVal: 1.181 ± 0.212
0.369MetTrp: 0.369 ± 0.151
0.812MetTyr: 0.812 ± 0.23
0.0MetXaa: 0.0 ± 0.0
Asn
3.764AsnAla: 3.764 ± 0.586
0.221AsnCys: 0.221 ± 0.128
4.133AsnAsp: 4.133 ± 0.511
4.281AsnGlu: 4.281 ± 0.682
1.993AsnPhe: 1.993 ± 0.439
4.724AsnGly: 4.724 ± 0.757
0.812AsnHis: 0.812 ± 0.191
4.06AsnIle: 4.06 ± 0.627
5.241AsnLys: 5.241 ± 0.642
5.314AsnLeu: 5.314 ± 0.586
1.845AsnMet: 1.845 ± 0.358
3.322AsnAsn: 3.322 ± 0.488
1.993AsnPro: 1.993 ± 0.366
2.583AsnGln: 2.583 ± 0.652
3.1AsnArg: 3.1 ± 0.53
3.395AsnSer: 3.395 ± 0.534
3.1AsnThr: 3.1 ± 0.431
3.838AsnVal: 3.838 ± 0.418
0.886AsnTrp: 0.886 ± 0.231
2.51AsnTyr: 2.51 ± 0.453
0.0AsnXaa: 0.0 ± 0.0
Pro
1.181ProAla: 1.181 ± 0.282
0.221ProCys: 0.221 ± 0.132
1.55ProAsp: 1.55 ± 0.379
2.362ProGlu: 2.362 ± 0.382
1.698ProPhe: 1.698 ± 0.349
1.402ProGly: 1.402 ± 0.357
0.96ProHis: 0.96 ± 0.278
1.402ProIle: 1.402 ± 0.317
2.288ProLys: 2.288 ± 0.391
2.288ProLeu: 2.288 ± 0.451
0.517ProMet: 0.517 ± 0.187
1.55ProAsn: 1.55 ± 0.334
0.812ProPro: 0.812 ± 0.182
1.107ProGln: 1.107 ± 0.323
1.107ProArg: 1.107 ± 0.258
1.845ProSer: 1.845 ± 0.354
0.886ProThr: 0.886 ± 0.251
1.845ProVal: 1.845 ± 0.402
0.295ProTrp: 0.295 ± 0.165
1.255ProTyr: 1.255 ± 0.24
0.0ProXaa: 0.0 ± 0.0
Gln
3.174GlnAla: 3.174 ± 0.496
0.148GlnCys: 0.148 ± 0.112
2.583GlnAsp: 2.583 ± 0.424
1.919GlnGlu: 1.919 ± 0.336
1.55GlnPhe: 1.55 ± 0.283
2.362GlnGly: 2.362 ± 0.385
1.107GlnHis: 1.107 ± 0.476
2.805GlnIle: 2.805 ± 0.451
3.543GlnLys: 3.543 ± 0.649
3.1GlnLeu: 3.1 ± 0.357
1.033GlnMet: 1.033 ± 0.287
2.879GlnAsn: 2.879 ± 0.351
0.738GlnPro: 0.738 ± 0.245
2.067GlnGln: 2.067 ± 0.424
1.993GlnArg: 1.993 ± 0.455
2.657GlnSer: 2.657 ± 0.365
2.362GlnThr: 2.362 ± 0.448
1.845GlnVal: 1.845 ± 0.374
0.369GlnTrp: 0.369 ± 0.15
1.845GlnTyr: 1.845 ± 0.301
0.0GlnXaa: 0.0 ± 0.0
Arg
2.657ArgAla: 2.657 ± 0.634
0.0ArgCys: 0.0 ± 0.0
2.51ArgAsp: 2.51 ± 0.554
3.248ArgGlu: 3.248 ± 0.447
2.362ArgPhe: 2.362 ± 0.326
2.436ArgGly: 2.436 ± 0.405
0.664ArgHis: 0.664 ± 0.191
2.952ArgIle: 2.952 ± 0.505
3.395ArgLys: 3.395 ± 0.408
4.429ArgLeu: 4.429 ± 0.534
0.96ArgMet: 0.96 ± 0.269
2.583ArgAsn: 2.583 ± 0.467
0.96ArgPro: 0.96 ± 0.213
1.993ArgGln: 1.993 ± 0.349
1.698ArgArg: 1.698 ± 0.398
2.141ArgSer: 2.141 ± 0.555
3.617ArgThr: 3.617 ± 0.472
2.583ArgVal: 2.583 ± 0.361
0.517ArgTrp: 0.517 ± 0.167
1.771ArgTyr: 1.771 ± 0.378
0.0ArgXaa: 0.0 ± 0.0
Ser
3.691SerAla: 3.691 ± 0.496
0.148SerCys: 0.148 ± 0.091
3.986SerAsp: 3.986 ± 0.794
4.724SerGlu: 4.724 ± 0.783
2.362SerPhe: 2.362 ± 0.443
4.429SerGly: 4.429 ± 0.945
0.886SerHis: 0.886 ± 0.2
4.65SerIle: 4.65 ± 0.8
5.831SerLys: 5.831 ± 0.554
3.838SerLeu: 3.838 ± 0.488
2.362SerMet: 2.362 ± 0.625
4.06SerAsn: 4.06 ± 0.751
1.181SerPro: 1.181 ± 0.27
1.993SerGln: 1.993 ± 0.388
2.141SerArg: 2.141 ± 0.541
4.06SerSer: 4.06 ± 0.702
2.952SerThr: 2.952 ± 0.536
3.838SerVal: 3.838 ± 0.495
0.886SerTrp: 0.886 ± 0.296
2.288SerTyr: 2.288 ± 0.555
0.0SerXaa: 0.0 ± 0.0
Thr
3.026ThrAla: 3.026 ± 0.629
0.369ThrCys: 0.369 ± 0.164
3.617ThrAsp: 3.617 ± 0.572
4.06ThrGlu: 4.06 ± 0.655
3.617ThrPhe: 3.617 ± 0.512
3.986ThrGly: 3.986 ± 0.653
1.771ThrHis: 1.771 ± 0.318
4.945ThrIle: 4.945 ± 0.777
4.872ThrLys: 4.872 ± 0.483
4.133ThrLeu: 4.133 ± 0.476
1.55ThrMet: 1.55 ± 0.618
3.1ThrAsn: 3.1 ± 0.469
1.993ThrPro: 1.993 ± 0.377
1.993ThrGln: 1.993 ± 0.39
2.436ThrArg: 2.436 ± 0.344
3.322ThrSer: 3.322 ± 0.635
3.838ThrThr: 3.838 ± 0.722
3.543ThrVal: 3.543 ± 0.489
0.812ThrTrp: 0.812 ± 0.245
2.141ThrTyr: 2.141 ± 0.655
0.0ThrXaa: 0.0 ± 0.0
Val
4.281ValAla: 4.281 ± 0.802
0.443ValCys: 0.443 ± 0.206
3.912ValAsp: 3.912 ± 0.528
4.281ValGlu: 4.281 ± 0.776
2.51ValPhe: 2.51 ± 0.483
3.469ValGly: 3.469 ± 0.518
0.517ValHis: 0.517 ± 0.177
3.986ValIle: 3.986 ± 0.433
5.905ValLys: 5.905 ± 0.764
4.872ValLeu: 4.872 ± 0.682
1.181ValMet: 1.181 ± 0.268
3.912ValAsn: 3.912 ± 0.539
1.845ValPro: 1.845 ± 0.443
1.919ValGln: 1.919 ± 0.36
3.026ValArg: 3.026 ± 0.456
3.764ValSer: 3.764 ± 0.549
3.469ValThr: 3.469 ± 0.489
3.838ValVal: 3.838 ± 0.555
0.517ValTrp: 0.517 ± 0.185
2.51ValTyr: 2.51 ± 0.422
0.0ValXaa: 0.0 ± 0.0
Trp
0.812TrpAla: 0.812 ± 0.298
0.0TrpCys: 0.0 ± 0.0
0.886TrpAsp: 0.886 ± 0.27
0.812TrpGlu: 0.812 ± 0.28
0.812TrpPhe: 0.812 ± 0.311
0.517TrpGly: 0.517 ± 0.169
0.148TrpHis: 0.148 ± 0.106
1.329TrpIle: 1.329 ± 0.295
0.886TrpLys: 0.886 ± 0.23
1.329TrpLeu: 1.329 ± 0.292
0.369TrpMet: 0.369 ± 0.193
0.886TrpAsn: 0.886 ± 0.336
0.443TrpPro: 0.443 ± 0.253
0.59TrpGln: 0.59 ± 0.177
0.59TrpArg: 0.59 ± 0.221
0.886TrpSer: 0.886 ± 0.292
0.738TrpThr: 0.738 ± 0.21
0.443TrpVal: 0.443 ± 0.151
0.148TrpTrp: 0.148 ± 0.09
0.738TrpTyr: 0.738 ± 0.204
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.805TyrAla: 2.805 ± 0.377
0.295TyrCys: 0.295 ± 0.136
2.879TyrAsp: 2.879 ± 0.539
3.764TyrGlu: 3.764 ± 0.64
1.402TyrPhe: 1.402 ± 0.406
3.026TyrGly: 3.026 ± 0.483
0.664TyrHis: 0.664 ± 0.249
2.657TyrIle: 2.657 ± 0.539
3.691TyrLys: 3.691 ± 0.505
3.617TyrLeu: 3.617 ± 0.462
1.55TyrMet: 1.55 ± 0.36
2.288TyrAsn: 2.288 ± 0.402
0.96TyrPro: 0.96 ± 0.25
1.698TyrGln: 1.698 ± 0.376
1.255TyrArg: 1.255 ± 0.259
2.436TyrSer: 2.436 ± 0.48
1.845TyrThr: 1.845 ± 0.557
2.288TyrVal: 2.288 ± 0.472
0.443TyrTrp: 0.443 ± 0.155
1.55TyrTyr: 1.55 ± 0.328
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (13549 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski