Mesorhizobium albiziae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6112 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4EUV0|A0A1I4EUV0_9RHIZ DNA-binding transcriptional regulator LysR family OS=Mesorhizobium albiziae OX=335020 GN=SAMN04488498_13034 PE=3 SV=1
MM1 pKa = 7.93DD2 pKa = 5.32FKK4 pKa = 11.57SLLLGSAAALVAVSAARR21 pKa = 11.84AADD24 pKa = 3.19AVVIPEE30 pKa = 4.5PEE32 pKa = 3.71PVEE35 pKa = 4.05YY36 pKa = 11.08VRR38 pKa = 11.84VCDD41 pKa = 3.84VYY43 pKa = 10.71GTAFYY48 pKa = 9.86YY49 pKa = 10.19IPGTEE54 pKa = 3.92TCLKK58 pKa = 8.47VSGYY62 pKa = 9.13VRR64 pKa = 11.84YY65 pKa = 10.19DD66 pKa = 2.94IGVGDD71 pKa = 4.45LFGLQSADD79 pKa = 3.46KK80 pKa = 10.68LDD82 pKa = 3.86LVEE85 pKa = 6.94DD86 pKa = 4.29GVLDD90 pKa = 4.54INDD93 pKa = 4.01TYY95 pKa = 11.13FKK97 pKa = 10.4RR98 pKa = 11.84ARR100 pKa = 11.84AALRR104 pKa = 11.84LDD106 pKa = 3.2ARR108 pKa = 11.84TEE110 pKa = 4.27TEE112 pKa = 4.07LGTLRR117 pKa = 11.84AYY119 pKa = 9.64TEE121 pKa = 5.88LKK123 pKa = 10.02FQWDD127 pKa = 4.1TNQDD131 pKa = 3.45SVDD134 pKa = 3.73VVDD137 pKa = 6.27PIAGDD142 pKa = 3.69TVTVPFVSAGNEE154 pKa = 3.67FAMEE158 pKa = 3.93DD159 pKa = 3.91VYY161 pKa = 11.29IEE163 pKa = 4.15LGGFRR168 pKa = 11.84IGATDD173 pKa = 3.46SLFTSFTDD181 pKa = 3.57YY182 pKa = 11.42AGAVINDD189 pKa = 4.33DD190 pKa = 3.73FLVPYY195 pKa = 10.33GPLDD199 pKa = 3.43GDD201 pKa = 3.66MTHH204 pKa = 6.59QIAYY208 pKa = 7.94TFTADD213 pKa = 3.47NGFTAAIAIEE223 pKa = 4.5EE224 pKa = 4.6GNGDD228 pKa = 3.72PDD230 pKa = 4.11AVFGSDD236 pKa = 3.16ADD238 pKa = 3.79GDD240 pKa = 4.5GVVDD244 pKa = 3.86TVTTIEE250 pKa = 3.93AAYY253 pKa = 9.13TLDD256 pKa = 4.48DD257 pKa = 4.63YY258 pKa = 11.33IPHH261 pKa = 5.65VVAGVGYY268 pKa = 9.78AGGWGSIKK276 pKa = 10.74AVGAYY281 pKa = 9.92DD282 pKa = 4.03AVWEE286 pKa = 4.22EE287 pKa = 3.69WAAKK291 pKa = 9.8VRR293 pKa = 11.84LDD295 pKa = 3.62VKK297 pKa = 10.14ATQTISAFIMAGYY310 pKa = 9.82KK311 pKa = 10.11SSEE314 pKa = 4.45DD315 pKa = 3.78FSITDD320 pKa = 3.71PVTGITTTFDD330 pKa = 3.19GPNYY334 pKa = 8.05YY335 pKa = 10.84GNWGGEE341 pKa = 3.67WAIWGGGSVEE351 pKa = 4.25VTEE354 pKa = 4.28KK355 pKa = 10.62ATFNVQLAYY364 pKa = 10.67DD365 pKa = 3.81DD366 pKa = 4.77FEE368 pKa = 4.79NFSAVANVAYY378 pKa = 9.7EE379 pKa = 4.05VVPGFMITPEE389 pKa = 3.59VAYY392 pKa = 10.32ADD394 pKa = 3.75NFEE397 pKa = 5.84DD398 pKa = 5.15SDD400 pKa = 5.01LDD402 pKa = 4.27DD403 pKa = 4.99FDD405 pKa = 6.41GEE407 pKa = 4.24FGGYY411 pKa = 10.05LRR413 pKa = 11.84FQRR416 pKa = 11.84NFF418 pKa = 3.01

Molecular weight: 45.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4FL32|A0A1I4FL32_9RHIZ Biotin carboxylase OS=Mesorhizobium albiziae OX=335020 GN=SAMN04488498_1491 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.47SQDD5 pKa = 3.45HH6 pKa = 6.27RR7 pKa = 11.84HH8 pKa = 5.15TAQARR13 pKa = 11.84QPTRR17 pKa = 11.84AGEE20 pKa = 4.18RR21 pKa = 11.84KK22 pKa = 9.15GCRR25 pKa = 11.84SSALGSPGRR34 pKa = 11.84RR35 pKa = 11.84SVEE38 pKa = 3.64DD39 pKa = 3.98PYY41 pKa = 11.44SVRR44 pKa = 11.84NLGSSLGISKK54 pKa = 9.06TEE56 pKa = 3.8VNASINRR63 pKa = 11.84SLASGIAARR72 pKa = 11.84DD73 pKa = 3.59RR74 pKa = 11.84DD75 pKa = 3.7TGRR78 pKa = 11.84AKK80 pKa = 10.44PSRR83 pKa = 11.84RR84 pKa = 11.84SLLNFIVHH92 pKa = 6.0GLKK95 pKa = 10.31FVFPAKK101 pKa = 9.87PGAMQRR107 pKa = 11.84GIPTAFAAPVLKK119 pKa = 10.61NALISAGSYY128 pKa = 9.73IYY130 pKa = 10.25VWPYY134 pKa = 11.59ASGHH138 pKa = 6.68DD139 pKa = 3.91MGQSVTPLFKK149 pKa = 10.63SVPEE153 pKa = 4.15SVQKK157 pKa = 10.67DD158 pKa = 3.43DD159 pKa = 5.0RR160 pKa = 11.84LYY162 pKa = 10.98EE163 pKa = 4.04YY164 pKa = 10.78LALADD169 pKa = 5.51AIRR172 pKa = 11.84LGNQRR177 pKa = 11.84EE178 pKa = 4.1AGLAAEE184 pKa = 4.69RR185 pKa = 11.84LSEE188 pKa = 3.9GLRR191 pKa = 11.84RR192 pKa = 11.84NDD194 pKa = 3.15QHH196 pKa = 6.6PRR198 pKa = 11.84PAACNAKK205 pKa = 9.61GCRR208 pKa = 11.84GSAGRR213 pKa = 11.84HH214 pKa = 3.92LRR216 pKa = 11.84NRR218 pKa = 11.84LVFVGGCTTALFITDD233 pKa = 3.88SVTLEE238 pKa = 4.06NVRR241 pKa = 11.84ATDD244 pKa = 3.98DD245 pKa = 3.14VDD247 pKa = 6.39LIVDD251 pKa = 4.3LAGFSEE257 pKa = 4.69WAQLPGGGLALGAARR272 pKa = 11.84GGGRR276 pKa = 11.84RR277 pKa = 11.84QQDD280 pKa = 3.15RR281 pKa = 11.84ARR283 pKa = 11.84RR284 pKa = 11.84RR285 pKa = 11.84CRR287 pKa = 11.84FVAAGIAARR296 pKa = 11.84TSGKK300 pKa = 9.25VLWCVTQQDD309 pKa = 3.82LFAPVAHH316 pKa = 6.18QAGLPPSRR324 pKa = 11.84VIHH327 pKa = 5.88VEE329 pKa = 3.63AGDD332 pKa = 3.78EE333 pKa = 4.22KK334 pKa = 10.37TVLGMEE340 pKa = 4.91EE341 pKa = 4.39GRR343 pKa = 11.84QSARR347 pKa = 11.84ARR349 pKa = 11.84PVYY352 pKa = 10.35NSRR355 pKa = 3.6

Molecular weight: 38.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6112

0

6112

1794598

39

2854

293.6

31.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.286 ± 0.045

0.819 ± 0.01

5.687 ± 0.024

5.809 ± 0.029

3.909 ± 0.022

8.491 ± 0.036

2.012 ± 0.016

5.371 ± 0.024

3.723 ± 0.023

9.797 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.015

2.752 ± 0.019

5.062 ± 0.025

3.006 ± 0.017

6.998 ± 0.035

5.514 ± 0.019

5.223 ± 0.024

7.428 ± 0.025

1.347 ± 0.013

2.251 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski