Capybara microvirus Cap3_SP_443
Average proteome isoelectric point is 5.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8L3|A0A4P8W8L3_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_443 OX=2585455 PE=4 SV=1
MM1 pKa = 7.29 KK2 pKa = 10.54 VKK4 pKa = 10.21 IYY6 pKa = 10.58 SAYY9 pKa = 10.36 DD10 pKa = 3.38 LPKK13 pKa = 10.15 PSEE16 pKa = 4.06 LTGFSPIEE24 pKa = 3.83 KK25 pKa = 9.42 TYY27 pKa = 10.11 MEE29 pKa = 4.87 QYY31 pKa = 10.61 NKK33 pKa = 10.92 DD34 pKa = 3.53 GIKK37 pKa = 10.21 EE38 pKa = 3.98 IIEE41 pKa = 4.01 VGEE44 pKa = 4.39 TNWYY48 pKa = 10.9 AEE50 pKa = 4.29 VQSYY54 pKa = 11.56 ADD56 pKa = 3.49 EE57 pKa = 4.4 TDD59 pKa = 3.14 VNKK62 pKa = 10.43 IMARR66 pKa = 11.84 VAGGEE71 pKa = 4.13 TGLLDD76 pKa = 3.75 QMKK79 pKa = 10.4 GFYY82 pKa = 10.4 LDD84 pKa = 3.75 TQGLPASLMEE94 pKa = 4.37 AQNIILKK101 pKa = 8.67 GQKK104 pKa = 10.45 AFDD107 pKa = 3.91 NLPAEE112 pKa = 4.43 IRR114 pKa = 11.84 RR115 pKa = 11.84 MYY117 pKa = 11.22 DD118 pKa = 2.78 NDD120 pKa = 3.21 AMKK123 pKa = 10.67 FMANFDD129 pKa = 3.71 YY130 pKa = 11.31 DD131 pKa = 3.74 QVAKK135 pKa = 10.77 NLEE138 pKa = 4.01 EE139 pKa = 4.2 VQAGKK144 pKa = 10.52 AVTQAVKK151 pKa = 10.74 EE152 pKa = 4.23 GGNDD156 pKa = 3.22 NAEE159 pKa = 4.2 NAA161 pKa = 4.07
Molecular weight: 18.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.553
IPC2_protein 4.52
IPC_protein 4.431
Toseland 4.253
ProMoST 4.533
Dawson 4.368
Bjellqvist 4.52
Wikipedia 4.24
Rodwell 4.253
Grimsley 4.164
Solomon 4.368
Lehninger 4.317
Nozaki 4.482
DTASelect 4.635
Thurlkill 4.266
EMBOSS 4.253
Sillero 4.533
Patrickios 3.872
IPC_peptide 4.368
IPC2_peptide 4.52
IPC2.peptide.svr19 4.492
Protein with the highest isoelectric point:
>tr|A0A4P8W8L3|A0A4P8W8L3_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_443 OX=2585455 PE=4 SV=1
MM1 pKa = 7.74 ACQKK5 pKa = 9.24 PLCFIEE11 pKa = 4.27 YY12 pKa = 9.04 PGEE15 pKa = 3.84 RR16 pKa = 11.84 RR17 pKa = 11.84 KK18 pKa = 9.95 ILPARR23 pKa = 11.84 IWDD26 pKa = 3.81 EE27 pKa = 3.72 YY28 pKa = 11.12 LAAKK32 pKa = 10.11 RR33 pKa = 11.84 DD34 pKa = 3.7 GDD36 pKa = 3.94 EE37 pKa = 4.33 EE38 pKa = 4.44 TLSRR42 pKa = 11.84 APYY45 pKa = 8.63 YY46 pKa = 10.44 GATRR50 pKa = 11.84 VVQIPCGQCIQCRR63 pKa = 11.84 LDD65 pKa = 4.9 KK66 pKa = 10.67 SRR68 pKa = 11.84 KK69 pKa = 6.06 WANRR73 pKa = 11.84 MMAEE77 pKa = 4.16 ARR79 pKa = 11.84 CWDD82 pKa = 3.31 HH83 pKa = 7.45 NYY85 pKa = 9.79 FVTLTYY91 pKa = 10.94 DD92 pKa = 3.87 DD93 pKa = 3.68 NHH95 pKa = 7.74 LPIRR99 pKa = 11.84 DD100 pKa = 3.62 WFHH103 pKa = 7.93 DD104 pKa = 3.77 EE105 pKa = 4.94 DD106 pKa = 5.17 GSIQEE111 pKa = 4.81 GVTLLPDD118 pKa = 4.77 EE119 pKa = 4.83 ISKK122 pKa = 10.18 FMKK125 pKa = 10.22 KK126 pKa = 10.07 LRR128 pKa = 11.84 VYY130 pKa = 9.38 YY131 pKa = 9.85 EE132 pKa = 3.71 RR133 pKa = 11.84 RR134 pKa = 11.84 TGKK137 pKa = 8.51 TGIRR141 pKa = 11.84 FFGCGEE147 pKa = 3.95 YY148 pKa = 10.83 GEE150 pKa = 4.75 KK151 pKa = 9.91 SGRR154 pKa = 11.84 PHH156 pKa = 4.84 YY157 pKa = 10.48 HH158 pKa = 6.7 IILFNCEE165 pKa = 4.09 LDD167 pKa = 3.59 DD168 pKa = 4.64 LKK170 pKa = 10.88 PYY172 pKa = 10.34 KK173 pKa = 9.93 ATEE176 pKa = 4.25 CGPLFNSAFLEE187 pKa = 4.74 SIWSDD192 pKa = 3.3 EE193 pKa = 4.18 YY194 pKa = 10.84 GQKK197 pKa = 10.86 GYY199 pKa = 8.54 VTVAPFTWDD208 pKa = 2.51 TGAYY212 pKa = 7.04 VARR215 pKa = 11.84 YY216 pKa = 9.23 IMKK219 pKa = 9.96 KK220 pKa = 9.54 QLGPPAYY227 pKa = 9.91 SYY229 pKa = 11.58 YY230 pKa = 10.36 NEE232 pKa = 3.93 HH233 pKa = 6.65 RR234 pKa = 11.84 KK235 pKa = 9.4 IPEE238 pKa = 3.7 YY239 pKa = 11.1 VIMSRR244 pKa = 11.84 NPGIGKK250 pKa = 9.79 FYY252 pKa = 10.78 YY253 pKa = 9.88 DD254 pKa = 3.32 QKK256 pKa = 11.11 KK257 pKa = 10.88 DD258 pKa = 3.64 EE259 pKa = 4.57 IYY261 pKa = 9.26 EE262 pKa = 3.84 TDD264 pKa = 3.42 EE265 pKa = 5.05 IIIHH269 pKa = 5.62 KK270 pKa = 10.23 HH271 pKa = 4.3 GATIKK276 pKa = 10.35 AKK278 pKa = 9.39 PGKK281 pKa = 10.49 YY282 pKa = 9.67 FDD284 pKa = 4.18 GYY286 pKa = 10.09 YY287 pKa = 10.46 AAEE290 pKa = 4.39 EE291 pKa = 4.29 PEE293 pKa = 3.95 KK294 pKa = 10.82 MEE296 pKa = 4.07 VIKK299 pKa = 10.59 RR300 pKa = 11.84 KK301 pKa = 9.66 RR302 pKa = 11.84 EE303 pKa = 3.55 RR304 pKa = 11.84 AGYY307 pKa = 9.08 MSEE310 pKa = 4.19 LEE312 pKa = 3.98 QRR314 pKa = 11.84 RR315 pKa = 11.84 QSGLTAQEE323 pKa = 3.97 YY324 pKa = 10.42 RR325 pKa = 11.84 DD326 pKa = 3.8 LQAEE330 pKa = 4.29 SMARR334 pKa = 11.84 RR335 pKa = 11.84 MAGLSRR341 pKa = 11.84 DD342 pKa = 3.38 LDD344 pKa = 3.72
Molecular weight: 40.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.817
IPC2_protein 7.088
IPC_protein 7.044
Toseland 6.795
ProMoST 7.439
Dawson 7.688
Bjellqvist 7.951
Wikipedia 7.614
Rodwell 7.702
Grimsley 6.854
Solomon 7.746
Lehninger 7.761
Nozaki 8.112
DTASelect 7.834
Thurlkill 7.878
EMBOSS 7.922
Sillero 8.156
Patrickios 3.948
IPC_peptide 7.746
IPC2_peptide 7.22
IPC2.peptide.svr19 7.253
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1466
79
625
293.2
32.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.458 ± 2.108
0.75 ± 0.476
5.866 ± 0.822
7.094 ± 0.643
3.888 ± 0.744
8.595 ± 0.738
1.842 ± 0.287
4.911 ± 0.597
6.071 ± 0.642
6.003 ± 0.804
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.411 ± 0.267
4.638 ± 0.827
4.025 ± 0.822
4.57 ± 0.339
4.707 ± 0.927
7.094 ± 1.646
5.662 ± 0.695
5.457 ± 0.732
1.569 ± 0.29
5.389 ± 0.647
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here