Arthrobacter sp. PAMC 25486

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4345 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1D2R9|A0A0A1D2R9_9MICC Cytochrome c biogenesis protein CcdA OS=Arthrobacter sp. PAMC 25486 OX=1494608 GN=ART_3115 PE=4 SV=1
MM1 pKa = 7.72GEE3 pKa = 4.48HH4 pKa = 7.8PIQSSDD10 pKa = 2.98NSAAPEE16 pKa = 4.6GGQQPGNQPQPEE28 pKa = 4.19NQQPEE33 pKa = 4.11QFAAQPGGLPPPFAQPPFYY52 pKa = 9.86PPPFAQPGDD61 pKa = 3.65QQGPYY66 pKa = 8.38PQSPHH71 pKa = 5.41SQPYY75 pKa = 8.39PPYY78 pKa = 9.91GAPALKK84 pKa = 10.06QDD86 pKa = 3.98TTNTMAIVAFVLSFVVNLAGVITGHH111 pKa = 6.85IALGQIKK118 pKa = 8.36RR119 pKa = 11.84TGEE122 pKa = 3.82KK123 pKa = 10.62GRR125 pKa = 11.84GFALAGVIIGYY136 pKa = 9.96VSLAASVAAAIILVVVLAAGGLAASGVTSYY166 pKa = 11.73DD167 pKa = 3.23PDD169 pKa = 4.72AEE171 pKa = 4.28SARR174 pKa = 11.84PNGTVEE180 pKa = 4.06QVNTNAYY187 pKa = 10.19GGITFGRR194 pKa = 11.84DD195 pKa = 2.9GVLIPPTVLTADD207 pKa = 3.24VDD209 pKa = 3.27IWDD212 pKa = 4.52LPKK215 pKa = 10.87EE216 pKa = 4.15PASLADD222 pKa = 3.38IGIKK226 pKa = 10.32ASASGEE232 pKa = 4.0PVQVVVYY239 pKa = 10.29LDD241 pKa = 4.78FMCPDD246 pKa = 3.51CADD249 pKa = 3.58FEE251 pKa = 4.65EE252 pKa = 5.54EE253 pKa = 4.25YY254 pKa = 11.14GPSLRR259 pKa = 11.84ALGDD263 pKa = 3.33QGGVTLEE270 pKa = 3.8YY271 pKa = 10.62RR272 pKa = 11.84PGGFLDD278 pKa = 4.54EE279 pKa = 4.67YY280 pKa = 11.26SDD282 pKa = 3.91GADD285 pKa = 3.37YY286 pKa = 11.05SSRR289 pKa = 11.84AAEE292 pKa = 4.06AAACVANTAPEE303 pKa = 4.33DD304 pKa = 3.57YY305 pKa = 10.65KK306 pKa = 11.29RR307 pKa = 11.84FIDD310 pKa = 3.85TLLANQPAIDD320 pKa = 4.69SMMPDD325 pKa = 2.97TNYY328 pKa = 10.73LVEE331 pKa = 4.21LANAVGAGDD340 pKa = 5.22IDD342 pKa = 4.31DD343 pKa = 5.3CLAGMDD349 pKa = 4.1MFDD352 pKa = 4.09LVSGATLLAVGHH364 pKa = 6.65GLTGIPGVFMDD375 pKa = 4.77GKK377 pKa = 10.38QWMDD381 pKa = 3.37GPFPDD386 pKa = 5.03FAQGVLDD393 pKa = 4.63AKK395 pKa = 10.61QQ396 pKa = 2.8

Molecular weight: 41.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1CRI7|A0A0A1CRI7_9MICC Arylsulfatase OS=Arthrobacter sp. PAMC 25486 OX=1494608 GN=ART_0141 PE=4 SV=1
MM1 pKa = 7.59ALGRR5 pKa = 11.84QVPPSFLHH13 pKa = 5.95ARR15 pKa = 11.84KK16 pKa = 7.42TVLGRR21 pKa = 11.84ILRR24 pKa = 11.84LHH26 pKa = 7.07PAQKK30 pKa = 10.18LVARR34 pKa = 11.84RR35 pKa = 11.84FTRR38 pKa = 11.84QQ39 pKa = 2.67

Molecular weight: 4.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4345

0

4345

1309562

37

2773

301.4

32.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.074 ± 0.053

0.623 ± 0.009

5.434 ± 0.027

5.449 ± 0.03

3.31 ± 0.021

8.947 ± 0.034

2.156 ± 0.017

4.583 ± 0.029

2.918 ± 0.027

10.253 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.017

2.699 ± 0.022

5.357 ± 0.027

3.173 ± 0.023

5.879 ± 0.036

6.017 ± 0.026

6.058 ± 0.033

8.28 ± 0.032

1.469 ± 0.017

2.044 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski