Amino acid dipepetide frequency for Arthrobacter sp. PAMC 25486

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.576AlaAla: 20.576 ± 0.235
0.76AlaCys: 0.76 ± 0.025
6.792AlaAsp: 6.792 ± 0.077
7.497AlaGlu: 7.497 ± 0.08
3.72AlaPhe: 3.72 ± 0.051
12.877AlaGly: 12.877 ± 0.13
2.51AlaHis: 2.51 ± 0.05
5.379AlaIle: 5.379 ± 0.071
4.014AlaLys: 4.014 ± 0.066
13.364AlaLeu: 13.364 ± 0.128
3.199AlaMet: 3.199 ± 0.052
3.054AlaAsn: 3.054 ± 0.049
6.297AlaPro: 6.297 ± 0.099
4.339AlaGln: 4.339 ± 0.064
7.064AlaArg: 7.064 ± 0.086
6.911AlaSer: 6.911 ± 0.082
7.341AlaThr: 7.341 ± 0.083
11.146AlaVal: 11.146 ± 0.113
1.665AlaTrp: 1.665 ± 0.035
2.24AlaTyr: 2.24 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
0.797CysAla: 0.797 ± 0.028
0.06CysCys: 0.06 ± 0.008
0.342CysAsp: 0.342 ± 0.015
0.3CysGlu: 0.3 ± 0.013
0.22CysPhe: 0.22 ± 0.012
0.696CysGly: 0.696 ± 0.023
0.153CysHis: 0.153 ± 0.011
0.287CysIle: 0.287 ± 0.016
0.126CysLys: 0.126 ± 0.01
0.515CysLeu: 0.515 ± 0.02
0.128CysMet: 0.128 ± 0.009
0.159CysAsn: 0.159 ± 0.012
0.347CysPro: 0.347 ± 0.019
0.186CysGln: 0.186 ± 0.013
0.353CysArg: 0.353 ± 0.017
0.443CysSer: 0.443 ± 0.022
0.435CysThr: 0.435 ± 0.02
0.445CysVal: 0.445 ± 0.016
0.107CysTrp: 0.107 ± 0.008
0.13CysTyr: 0.13 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
7.141AspAla: 7.141 ± 0.084
0.297AspCys: 0.297 ± 0.015
2.93AspAsp: 2.93 ± 0.05
3.204AspGlu: 3.204 ± 0.059
1.961AspPhe: 1.961 ± 0.038
5.732AspGly: 5.732 ± 0.087
1.162AspHis: 1.162 ± 0.03
2.489AspIle: 2.489 ± 0.046
1.468AspLys: 1.468 ± 0.035
5.15AspLeu: 5.15 ± 0.068
1.113AspMet: 1.113 ± 0.03
1.197AspAsn: 1.197 ± 0.028
3.571AspPro: 3.571 ± 0.058
1.494AspGln: 1.494 ± 0.033
2.877AspArg: 2.877 ± 0.056
3.001AspSer: 3.001 ± 0.057
2.739AspThr: 2.739 ± 0.049
4.644AspVal: 4.644 ± 0.069
0.899AspTrp: 0.899 ± 0.03
1.275AspTyr: 1.275 ± 0.032
0.0AspXaa: 0.0 ± 0.0
Glu
6.588GluAla: 6.588 ± 0.08
0.315GluCys: 0.315 ± 0.015
2.651GluAsp: 2.651 ± 0.048
2.911GluGlu: 2.911 ± 0.058
1.839GluPhe: 1.839 ± 0.041
3.694GluGly: 3.694 ± 0.063
1.48GluHis: 1.48 ± 0.033
2.735GluIle: 2.735 ± 0.056
1.787GluLys: 1.787 ± 0.04
7.036GluLeu: 7.036 ± 0.081
1.056GluMet: 1.056 ± 0.029
1.643GluAsn: 1.643 ± 0.031
2.788GluPro: 2.788 ± 0.055
2.315GluGln: 2.315 ± 0.044
3.652GluArg: 3.652 ± 0.062
3.138GluSer: 3.138 ± 0.058
3.017GluThr: 3.017 ± 0.049
4.027GluVal: 4.027 ± 0.061
0.765GluTrp: 0.765 ± 0.026
1.056GluTyr: 1.056 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
4.145PheAla: 4.145 ± 0.058
0.233PheCys: 0.233 ± 0.016
2.139PheAsp: 2.139 ± 0.045
1.672PheGlu: 1.672 ± 0.042
1.229PhePhe: 1.229 ± 0.036
3.375PheGly: 3.375 ± 0.061
0.673PheHis: 0.673 ± 0.024
1.507PheIle: 1.507 ± 0.033
0.9PheLys: 0.9 ± 0.029
3.142PheLeu: 3.142 ± 0.058
0.714PheMet: 0.714 ± 0.026
0.974PheAsn: 0.974 ± 0.025
1.575PhePro: 1.575 ± 0.034
0.861PheGln: 0.861 ± 0.024
1.628PheArg: 1.628 ± 0.038
2.293PheSer: 2.293 ± 0.045
2.315PheThr: 2.315 ± 0.04
2.524PheVal: 2.524 ± 0.052
0.483PheTrp: 0.483 ± 0.021
0.724PheTyr: 0.724 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
10.533GlyAla: 10.533 ± 0.123
0.627GlyCys: 0.627 ± 0.026
4.156GlyAsp: 4.156 ± 0.063
4.598GlyGlu: 4.598 ± 0.07
3.284GlyPhe: 3.284 ± 0.061
7.638GlyGly: 7.638 ± 0.108
2.028GlyHis: 2.028 ± 0.04
4.753GlyIle: 4.753 ± 0.059
2.928GlyLys: 2.928 ± 0.047
9.04GlyLeu: 9.04 ± 0.094
2.3GlyMet: 2.3 ± 0.045
2.404GlyAsn: 2.404 ± 0.049
3.956GlyPro: 3.956 ± 0.059
3.0GlyGln: 3.0 ± 0.047
5.318GlyArg: 5.318 ± 0.076
5.625GlySer: 5.625 ± 0.077
6.217GlyThr: 6.217 ± 0.077
7.276GlyVal: 7.276 ± 0.082
1.588GlyTrp: 1.588 ± 0.038
2.2GlyTyr: 2.2 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
2.348HisAla: 2.348 ± 0.045
0.157HisCys: 0.157 ± 0.01
1.297HisAsp: 1.297 ± 0.036
1.163HisGlu: 1.163 ± 0.028
0.738HisPhe: 0.738 ± 0.024
2.15HisGly: 2.15 ± 0.043
0.655HisHis: 0.655 ± 0.023
0.892HisIle: 0.892 ± 0.028
0.515HisLys: 0.515 ± 0.02
2.094HisLeu: 2.094 ± 0.042
0.457HisMet: 0.457 ± 0.016
0.555HisAsn: 0.555 ± 0.02
1.478HisPro: 1.478 ± 0.034
0.729HisGln: 0.729 ± 0.025
1.439HisArg: 1.439 ± 0.036
1.242HisSer: 1.242 ± 0.034
1.113HisThr: 1.113 ± 0.03
1.671HisVal: 1.671 ± 0.038
0.368HisTrp: 0.368 ± 0.02
0.502HisTyr: 0.502 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
6.089IleAla: 6.089 ± 0.082
0.352IleCys: 0.352 ± 0.016
2.866IleAsp: 2.866 ± 0.051
2.418IleGlu: 2.418 ± 0.054
1.581IlePhe: 1.581 ± 0.039
4.196IleGly: 4.196 ± 0.061
0.905IleHis: 0.905 ± 0.027
2.119IleIle: 2.119 ± 0.046
1.326IleLys: 1.326 ± 0.033
4.086IleLeu: 4.086 ± 0.064
1.005IleMet: 1.005 ± 0.032
1.406IleAsn: 1.406 ± 0.034
2.537IlePro: 2.537 ± 0.05
1.174IleGln: 1.174 ± 0.029
2.496IleArg: 2.496 ± 0.045
2.983IleSer: 2.983 ± 0.047
2.945IleThr: 2.945 ± 0.062
3.896IleVal: 3.896 ± 0.066
0.525IleTrp: 0.525 ± 0.021
0.924IleTyr: 0.924 ± 0.028
0.001IleXaa: 0.001 ± 0.001
Lys
3.637LysAla: 3.637 ± 0.073
0.123LysCys: 0.123 ± 0.01
1.704LysAsp: 1.704 ± 0.046
1.474LysGlu: 1.474 ± 0.037
0.966LysPhe: 0.966 ± 0.027
1.977LysGly: 1.977 ± 0.039
0.704LysHis: 0.704 ± 0.022
1.45LysIle: 1.45 ± 0.039
1.178LysLys: 1.178 ± 0.037
2.875LysLeu: 2.875 ± 0.051
0.748LysMet: 0.748 ± 0.03
0.987LysAsn: 0.987 ± 0.028
1.658LysPro: 1.658 ± 0.045
0.829LysGln: 0.829 ± 0.027
1.714LysArg: 1.714 ± 0.044
1.761LysSer: 1.761 ± 0.042
1.841LysThr: 1.841 ± 0.045
2.444LysVal: 2.444 ± 0.047
0.367LysTrp: 0.367 ± 0.02
0.744LysTyr: 0.744 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
14.845LeuAla: 14.845 ± 0.141
0.686LeuCys: 0.686 ± 0.024
5.828LeuAsp: 5.828 ± 0.072
5.399LeuGlu: 5.399 ± 0.069
3.041LeuPhe: 3.041 ± 0.062
9.163LeuGly: 9.163 ± 0.088
2.098LeuHis: 2.098 ± 0.044
4.482LeuIle: 4.482 ± 0.071
2.645LeuLys: 2.645 ± 0.048
10.519LeuLeu: 10.519 ± 0.138
2.174LeuMet: 2.174 ± 0.045
2.678LeuAsn: 2.678 ± 0.047
5.822LeuPro: 5.822 ± 0.069
2.817LeuGln: 2.817 ± 0.045
6.346LeuArg: 6.346 ± 0.081
6.381LeuSer: 6.381 ± 0.077
6.137LeuThr: 6.137 ± 0.074
8.465LeuVal: 8.465 ± 0.096
1.32LeuTrp: 1.32 ± 0.035
1.686LeuTyr: 1.686 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
3.03MetAla: 3.03 ± 0.049
0.14MetCys: 0.14 ± 0.01
1.215MetAsp: 1.215 ± 0.029
1.081MetGlu: 1.081 ± 0.031
0.697MetPhe: 0.697 ± 0.023
1.926MetGly: 1.926 ± 0.037
0.425MetHis: 0.425 ± 0.021
1.008MetIle: 1.008 ± 0.03
0.7MetLys: 0.7 ± 0.024
2.24MetLeu: 2.24 ± 0.049
0.514MetMet: 0.514 ± 0.023
0.763MetAsn: 0.763 ± 0.026
1.218MetPro: 1.218 ± 0.03
0.576MetGln: 0.576 ± 0.022
1.262MetArg: 1.262 ± 0.033
1.667MetSer: 1.667 ± 0.034
1.708MetThr: 1.708 ± 0.037
1.921MetVal: 1.921 ± 0.041
0.281MetTrp: 0.281 ± 0.015
0.384MetTyr: 0.384 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.155AsnAla: 3.155 ± 0.055
0.196AsnCys: 0.196 ± 0.011
1.503AsnAsp: 1.503 ± 0.035
1.299AsnGlu: 1.299 ± 0.031
0.943AsnPhe: 0.943 ± 0.031
2.489AsnGly: 2.489 ± 0.051
0.596AsnHis: 0.596 ± 0.022
1.278AsnIle: 1.278 ± 0.034
0.812AsnLys: 0.812 ± 0.028
2.408AsnLeu: 2.408 ± 0.045
0.593AsnMet: 0.593 ± 0.018
0.903AsnAsn: 0.903 ± 0.029
1.919AsnPro: 1.919 ± 0.036
0.909AsnGln: 0.909 ± 0.026
1.491AsnArg: 1.491 ± 0.039
1.596AsnSer: 1.596 ± 0.038
1.686AsnThr: 1.686 ± 0.037
2.1AsnVal: 2.1 ± 0.044
0.4AsnTrp: 0.4 ± 0.018
0.711AsnTyr: 0.711 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
7.695ProAla: 7.695 ± 0.096
0.231ProCys: 0.231 ± 0.014
3.368ProAsp: 3.368 ± 0.061
3.917ProGlu: 3.917 ± 0.062
1.688ProPhe: 1.688 ± 0.037
5.18ProGly: 5.18 ± 0.063
1.174ProHis: 1.174 ± 0.033
1.891ProIle: 1.891 ± 0.035
1.384ProLys: 1.384 ± 0.034
4.924ProLeu: 4.924 ± 0.058
1.174ProMet: 1.174 ± 0.031
1.349ProAsn: 1.349 ± 0.031
2.18ProPro: 2.18 ± 0.057
1.701ProGln: 1.701 ± 0.041
2.647ProArg: 2.647 ± 0.047
3.305ProSer: 3.305 ± 0.056
3.226ProThr: 3.226 ± 0.068
4.689ProVal: 4.689 ± 0.069
0.825ProTrp: 0.825 ± 0.026
1.018ProTyr: 1.018 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.975GlnAla: 3.975 ± 0.06
0.192GlnCys: 0.192 ± 0.012
1.612GlnAsp: 1.612 ± 0.038
1.755GlnGlu: 1.755 ± 0.04
0.971GlnPhe: 0.971 ± 0.029
2.519GlnGly: 2.519 ± 0.054
0.723GlnHis: 0.723 ± 0.023
1.49GlnIle: 1.49 ± 0.036
0.959GlnLys: 0.959 ± 0.03
3.801GlnLeu: 3.801 ± 0.063
0.706GlnMet: 0.706 ± 0.026
0.794GlnAsn: 0.794 ± 0.025
1.657GlnPro: 1.657 ± 0.037
1.405GlnGln: 1.405 ± 0.041
2.29GlnArg: 2.29 ± 0.048
1.681GlnSer: 1.681 ± 0.043
1.581GlnThr: 1.581 ± 0.036
2.418GlnVal: 2.418 ± 0.041
0.551GlnTrp: 0.551 ± 0.021
0.648GlnTyr: 0.648 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
6.398ArgAla: 6.398 ± 0.084
0.346ArgCys: 0.346 ± 0.016
3.139ArgAsp: 3.139 ± 0.054
3.497ArgGlu: 3.497 ± 0.059
1.946ArgPhe: 1.946 ± 0.036
4.359ArgGly: 4.359 ± 0.068
1.464ArgHis: 1.464 ± 0.034
3.036ArgIle: 3.036 ± 0.051
1.905ArgLys: 1.905 ± 0.039
6.004ArgLeu: 6.004 ± 0.075
1.454ArgMet: 1.454 ± 0.034
1.795ArgAsn: 1.795 ± 0.036
2.934ArgPro: 2.934 ± 0.053
2.053ArgGln: 2.053 ± 0.044
4.678ArgArg: 4.678 ± 0.074
3.561ArgSer: 3.561 ± 0.053
3.606ArgThr: 3.606 ± 0.054
4.251ArgVal: 4.251 ± 0.066
1.014ArgTrp: 1.014 ± 0.031
1.349ArgTyr: 1.349 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
7.321SerAla: 7.321 ± 0.08
0.373SerCys: 0.373 ± 0.018
2.941SerAsp: 2.941 ± 0.049
2.906SerGlu: 2.906 ± 0.051
2.083SerPhe: 2.083 ± 0.04
6.01SerGly: 6.01 ± 0.074
1.231SerHis: 1.231 ± 0.03
2.752SerIle: 2.752 ± 0.058
1.73SerLys: 1.73 ± 0.034
6.02SerLeu: 6.02 ± 0.075
1.538SerMet: 1.538 ± 0.038
1.69SerAsn: 1.69 ± 0.038
3.319SerPro: 3.319 ± 0.058
1.862SerGln: 1.862 ± 0.035
3.448SerArg: 3.448 ± 0.053
3.778SerSer: 3.778 ± 0.057
3.872SerThr: 3.872 ± 0.056
4.746SerVal: 4.746 ± 0.062
1.038SerTrp: 1.038 ± 0.032
1.514SerTyr: 1.514 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
8.063ThrAla: 8.063 ± 0.094
0.312ThrCys: 0.312 ± 0.016
3.198ThrAsp: 3.198 ± 0.053
3.049ThrGlu: 3.049 ± 0.058
2.046ThrPhe: 2.046 ± 0.043
5.909ThrGly: 5.909 ± 0.07
1.206ThrHis: 1.206 ± 0.035
2.883ThrIle: 2.883 ± 0.053
1.605ThrLys: 1.605 ± 0.037
6.122ThrLeu: 6.122 ± 0.066
1.314ThrMet: 1.314 ± 0.028
1.472ThrAsn: 1.472 ± 0.038
3.783ThrPro: 3.783 ± 0.07
1.701ThrGln: 1.701 ± 0.037
3.011ThrArg: 3.011 ± 0.043
3.576ThrSer: 3.576 ± 0.057
3.838ThrThr: 3.838 ± 0.058
5.564ThrVal: 5.564 ± 0.068
0.807ThrTrp: 0.807 ± 0.026
1.126ThrTyr: 1.126 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
10.493ValAla: 10.493 ± 0.117
0.549ValCys: 0.549 ± 0.024
4.682ValAsp: 4.682 ± 0.064
4.581ValGlu: 4.581 ± 0.064
2.706ValPhe: 2.706 ± 0.052
6.575ValGly: 6.575 ± 0.076
1.71ValHis: 1.71 ± 0.037
3.962ValIle: 3.962 ± 0.061
2.231ValLys: 2.231 ± 0.049
9.144ValLeu: 9.144 ± 0.105
1.832ValMet: 1.832 ± 0.042
2.145ValAsn: 2.145 ± 0.04
4.732ValPro: 4.732 ± 0.068
2.41ValGln: 2.41 ± 0.038
4.71ValArg: 4.71 ± 0.057
5.079ValSer: 5.079 ± 0.058
4.953ValThr: 4.953 ± 0.074
7.875ValVal: 7.875 ± 0.092
0.996ValTrp: 0.996 ± 0.031
1.434ValTyr: 1.434 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.568TrpAla: 1.568 ± 0.038
0.118TrpCys: 0.118 ± 0.01
0.791TrpAsp: 0.791 ± 0.03
0.709TrpGlu: 0.709 ± 0.023
0.578TrpPhe: 0.578 ± 0.023
1.032TrpGly: 1.032 ± 0.025
0.354TrpHis: 0.354 ± 0.015
0.685TrpIle: 0.685 ± 0.023
0.397TrpLys: 0.397 ± 0.017
1.851TrpLeu: 1.851 ± 0.043
0.361TrpMet: 0.361 ± 0.015
0.481TrpAsn: 0.481 ± 0.021
0.696TrpPro: 0.696 ± 0.022
0.643TrpGln: 0.643 ± 0.023
1.016TrpArg: 1.016 ± 0.027
0.868TrpSer: 0.868 ± 0.029
0.841TrpThr: 0.841 ± 0.029
1.077TrpVal: 1.077 ± 0.032
0.332TrpTrp: 0.332 ± 0.016
0.296TrpTyr: 0.296 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.343TyrAla: 2.343 ± 0.041
0.162TyrCys: 0.162 ± 0.011
1.19TyrAsp: 1.19 ± 0.03
1.063TyrGlu: 1.063 ± 0.032
0.865TyrPhe: 0.865 ± 0.027
1.976TyrGly: 1.976 ± 0.042
0.312TyrHis: 0.312 ± 0.015
0.754TyrIle: 0.754 ± 0.024
0.567TyrLys: 0.567 ± 0.022
2.198TyrLeu: 2.198 ± 0.04
0.374TyrMet: 0.374 ± 0.018
0.539TyrAsn: 0.539 ± 0.023
1.1TyrPro: 1.1 ± 0.027
0.726TyrGln: 0.726 ± 0.025
1.351TyrArg: 1.351 ± 0.035
1.283TyrSer: 1.283 ± 0.034
1.175TyrThr: 1.175 ± 0.035
1.618TyrVal: 1.618 ± 0.036
0.363TyrTrp: 0.363 ± 0.017
0.482TyrTyr: 0.482 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.001XaaAla: 0.001 ± 0.001
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4345 proteins (1309562 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski