Acidiplasma cupricumulans
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1683 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q0XIK9|A0A0Q0XIK9_9ARCH Uncharacterized protein OS=Acidiplasma cupricumulans OX=312540 GN=AOG55_00690 PE=4 SV=1
MM1 pKa = 7.83 GDD3 pKa = 4.11 FIKK6 pKa = 10.67 KK7 pKa = 10.3 FEE9 pKa = 4.12 YY10 pKa = 11.03 LEE12 pKa = 4.46 DD13 pKa = 4.11 LNITLEE19 pKa = 3.77 LAYY22 pKa = 10.53 RR23 pKa = 11.84 LNYY26 pKa = 9.11 NFKK29 pKa = 8.26 GCGYY33 pKa = 9.67 IKK35 pKa = 10.46 VYY37 pKa = 10.04 SGKK40 pKa = 9.7 IDD42 pKa = 3.74 PEE44 pKa = 4.07 EE45 pKa = 3.97 EE46 pKa = 3.75 NYY48 pKa = 10.02 EE49 pKa = 3.99 IYY51 pKa = 9.83 MEE53 pKa = 4.51 SLDD56 pKa = 4.09 CGMSEE61 pKa = 5.36 DD62 pKa = 4.45 EE63 pKa = 5.46 VNSKK67 pKa = 8.43 YY68 pKa = 11.26 NKK70 pKa = 9.34 MIGEE74 pKa = 4.11 IRR76 pKa = 11.84 SGDD79 pKa = 3.24 IDD81 pKa = 4.33 LSLL84 pKa = 5.04
Molecular weight: 9.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.008
IPC2_protein 4.329
IPC_protein 4.215
Toseland 4.037
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.317
Wikipedia 4.037
Rodwell 4.05
Grimsley 3.961
Solomon 4.151
Lehninger 4.101
Nozaki 4.266
DTASelect 4.406
Thurlkill 4.062
EMBOSS 4.05
Sillero 4.317
Patrickios 1.964
IPC_peptide 4.151
IPC2_peptide 4.304
IPC2.peptide.svr19 4.225
Protein with the highest isoelectric point:
>tr|A0A0Q0XLZ3|A0A0Q0XLZ3_9ARCH Methylmalonate-semialdehyde dehydrogenase OS=Acidiplasma cupricumulans OX=312540 GN=AOG55_03945 PE=4 SV=1
MM1 pKa = 7.45 SRR3 pKa = 11.84 NKK5 pKa = 10.19 PLGKK9 pKa = 10.0 KK10 pKa = 9.39 LRR12 pKa = 11.84 LMNRR16 pKa = 11.84 IKK18 pKa = 10.79 SNRR21 pKa = 11.84 RR22 pKa = 11.84 VPGWVMLRR30 pKa = 11.84 TDD32 pKa = 3.1 RR33 pKa = 11.84 HH34 pKa = 4.16 VTQNPKK40 pKa = 9.45 RR41 pKa = 11.84 KK42 pKa = 8.31 NWRR45 pKa = 11.84 RR46 pKa = 11.84 SNLKK50 pKa = 10.1 LL51 pKa = 3.65
Molecular weight: 6.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.369
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.055
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1683
0
1683
497383
43
1698
295.5
33.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.725 ± 0.052
0.604 ± 0.018
5.544 ± 0.055
5.885 ± 0.066
4.97 ± 0.057
6.691 ± 0.049
1.494 ± 0.022
10.966 ± 0.068
7.542 ± 0.067
8.797 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.888 ± 0.027
6.535 ± 0.072
3.44 ± 0.034
1.702 ± 0.027
3.918 ± 0.039
6.961 ± 0.047
4.675 ± 0.038
5.732 ± 0.047
0.75 ± 0.018
5.181 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here