Blautia sp. An46

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Blautia; unclassified Blautia

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3536 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y3XX95|A0A1Y3XX95_9FIRM Aldehyde oxidoreductase OS=Blautia sp. An46 OX=1965636 GN=B5G00_16840 PE=3 SV=1
MM1 pKa = 6.76QKK3 pKa = 10.39KK4 pKa = 9.33YY5 pKa = 10.81LKK7 pKa = 10.43AVSALMCTAMCVSLAACGSGGGSDD31 pKa = 3.48EE32 pKa = 4.68GGSGSGTSGSGGLEE46 pKa = 3.17IAYY49 pKa = 10.58NLTTDD54 pKa = 3.83DD55 pKa = 4.13FSVFEE60 pKa = 5.35DD61 pKa = 4.11IINDD65 pKa = 3.59FSEE68 pKa = 4.18EE69 pKa = 3.84TGIDD73 pKa = 3.29VTIYY77 pKa = 10.97NGGDD81 pKa = 3.5DD82 pKa = 4.51YY83 pKa = 11.76EE84 pKa = 4.83SAMKK88 pKa = 10.03TRR90 pKa = 11.84MSSGDD95 pKa = 4.08LPDD98 pKa = 2.97MWVTHH103 pKa = 5.3GWSLIRR109 pKa = 11.84YY110 pKa = 8.23SEE112 pKa = 3.99YY113 pKa = 11.04MMDD116 pKa = 5.12LSDD119 pKa = 4.77QEE121 pKa = 4.43WVSNIDD127 pKa = 3.44PGLKK131 pKa = 9.85EE132 pKa = 5.31VITNDD137 pKa = 3.03NGEE140 pKa = 4.41LFILPITQAVAAIMYY155 pKa = 9.45NKK157 pKa = 10.28DD158 pKa = 3.03VLTQAGVDD166 pKa = 3.41ATQIRR171 pKa = 11.84TWDD174 pKa = 3.75DD175 pKa = 3.4FAAACQSVLDD185 pKa = 4.25NTDD188 pKa = 3.18AAPITGFF195 pKa = 3.64

Molecular weight: 20.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y3Y0Y7|A0A1Y3Y0Y7_9FIRM GNAT family N-acetyltransferase OS=Blautia sp. An46 OX=1965636 GN=B5G00_12720 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.51GGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3536

0

3536

1118543

37

2350

316.3

35.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.118 ± 0.044

1.49 ± 0.016

5.285 ± 0.035

8.292 ± 0.049

4.211 ± 0.029

7.24 ± 0.035

1.723 ± 0.017

7.149 ± 0.037

6.832 ± 0.037

9.368 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.022 ± 0.019

3.967 ± 0.026

3.477 ± 0.02

3.63 ± 0.026

4.785 ± 0.034

5.667 ± 0.033

5.133 ± 0.03

6.485 ± 0.034

0.973 ± 0.014

4.154 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski