Arthrobacter phage Hestia

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M3E3|A0A3G3M3E3_9CAUD Integrase OS=Arthrobacter phage Hestia OX=2419609 GN=38 PE=3 SV=1
MM1 pKa = 7.65SEE3 pKa = 4.6LNSPSKK9 pKa = 10.7FSGVTAAGLNDD20 pKa = 3.39WVAAQDD26 pKa = 3.64ADD28 pKa = 3.93GFPVDD33 pKa = 4.06GDD35 pKa = 3.63ADD37 pKa = 3.85LRR39 pKa = 11.84DD40 pKa = 3.88AAPQPEE46 pKa = 4.74TFYY49 pKa = 11.45CPAQTFAGRR58 pKa = 11.84YY59 pKa = 8.3YY60 pKa = 10.91VDD62 pKa = 4.02PEE64 pKa = 4.2PPEE67 pKa = 4.02HH68 pKa = 7.09CEE70 pKa = 4.19AEE72 pKa = 4.29VEE74 pKa = 4.47EE75 pKa = 4.77EE76 pKa = 4.49GQFCPAHH83 pKa = 6.54EE84 pKa = 4.58EE85 pKa = 3.92QDD87 pKa = 4.99DD88 pKa = 4.01YY89 pKa = 12.08NPWGNEE95 pKa = 3.47

Molecular weight: 10.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3M3G7|A0A3G3M3G7_9CAUD T5orf172 domain-containing protein OS=Arthrobacter phage Hestia OX=2419609 GN=86 PE=4 SV=1
MM1 pKa = 8.01RR2 pKa = 11.84GRR4 pKa = 11.84PPRR7 pKa = 11.84VKK9 pKa = 10.44KK10 pKa = 10.84EE11 pKa = 3.58EE12 pKa = 3.8FLRR15 pKa = 11.84LHH17 pKa = 6.62HH18 pKa = 6.9EE19 pKa = 4.47GLSAPQLAKK28 pKa = 10.73HH29 pKa = 6.15FDD31 pKa = 3.02VSQRR35 pKa = 11.84HH36 pKa = 4.51VARR39 pKa = 11.84LRR41 pKa = 11.84RR42 pKa = 11.84EE43 pKa = 3.88LGVARR48 pKa = 11.84DD49 pKa = 3.86TPEE52 pKa = 3.74ASHH55 pKa = 6.7RR56 pKa = 11.84VDD58 pKa = 4.31AVWLAKK64 pKa = 9.99AAQLLDD70 pKa = 4.07DD71 pKa = 4.88GAPVKK76 pKa = 10.13EE77 pKa = 4.14VAMTLGCTEE86 pKa = 3.89ATVNRR91 pKa = 11.84HH92 pKa = 4.31FPGRR96 pKa = 11.84GWDD99 pKa = 3.15HH100 pKa = 5.8STVGRR105 pKa = 11.84HH106 pKa = 4.65ARR108 pKa = 11.84AVRR111 pKa = 11.84TASEE115 pKa = 4.23EE116 pKa = 4.02LQLLHH121 pKa = 6.3ITPLYY126 pKa = 8.31TNRR129 pKa = 11.84RR130 pKa = 11.84YY131 pKa = 9.37PVV133 pKa = 2.88

Molecular weight: 15.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

15981

35

1325

179.6

19.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.82 ± 0.643

1.001 ± 0.16

6.445 ± 0.235

5.763 ± 0.282

2.797 ± 0.18

8.128 ± 0.36

2.303 ± 0.242

4.393 ± 0.16

4.737 ± 0.251

7.903 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.253 ± 0.113

3.392 ± 0.188

5.669 ± 0.358

3.617 ± 0.174

6.301 ± 0.343

5.588 ± 0.187

6.57 ± 0.265

6.958 ± 0.28

1.74 ± 0.152

2.622 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski