Amino acid dipepetide frequency for Arthrobacter phage Hestia

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.521AlaAla: 16.521 ± 1.423
0.814AlaCys: 0.814 ± 0.211
8.448AlaAsp: 8.448 ± 0.638
7.947AlaGlu: 7.947 ± 0.736
2.315AlaPhe: 2.315 ± 0.364
10.826AlaGly: 10.826 ± 1.166
2.566AlaHis: 2.566 ± 0.52
4.506AlaIle: 4.506 ± 0.557
6.258AlaLys: 6.258 ± 0.65
9.574AlaLeu: 9.574 ± 0.885
3.442AlaMet: 3.442 ± 0.577
4.005AlaAsn: 4.005 ± 0.499
5.257AlaPro: 5.257 ± 0.584
4.506AlaGln: 4.506 ± 0.624
6.633AlaArg: 6.633 ± 0.693
5.82AlaSer: 5.82 ± 0.889
6.821AlaThr: 6.821 ± 0.645
7.697AlaVal: 7.697 ± 0.7
2.441AlaTrp: 2.441 ± 0.423
1.815AlaTyr: 1.815 ± 0.303
0.0AlaXaa: 0.0 ± 0.0
Cys
0.876CysAla: 0.876 ± 0.223
0.25CysCys: 0.25 ± 0.133
0.876CysAsp: 0.876 ± 0.239
0.626CysGlu: 0.626 ± 0.178
0.063CysPhe: 0.063 ± 0.062
0.939CysGly: 0.939 ± 0.303
0.313CysHis: 0.313 ± 0.12
0.188CysIle: 0.188 ± 0.101
0.688CysLys: 0.688 ± 0.225
0.313CysLeu: 0.313 ± 0.143
0.0CysMet: 0.0 ± 0.0
0.375CysAsn: 0.375 ± 0.14
1.377CysPro: 1.377 ± 0.29
0.25CysGln: 0.25 ± 0.118
0.563CysArg: 0.563 ± 0.221
0.688CysSer: 0.688 ± 0.263
0.626CysThr: 0.626 ± 0.178
0.501CysVal: 0.501 ± 0.16
0.313CysTrp: 0.313 ± 0.133
0.188CysTyr: 0.188 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
8.073AspAla: 8.073 ± 0.679
0.814AspCys: 0.814 ± 0.223
4.255AspAsp: 4.255 ± 0.761
3.317AspGlu: 3.317 ± 0.534
1.627AspPhe: 1.627 ± 0.305
6.633AspGly: 6.633 ± 0.689
1.377AspHis: 1.377 ± 0.318
3.317AspIle: 3.317 ± 0.37
2.441AspLys: 2.441 ± 0.316
5.069AspLeu: 5.069 ± 0.541
1.252AspMet: 1.252 ± 0.276
1.564AspAsn: 1.564 ± 0.331
4.443AspPro: 4.443 ± 0.543
1.815AspGln: 1.815 ± 0.397
4.193AspArg: 4.193 ± 0.473
3.317AspSer: 3.317 ± 0.475
3.066AspThr: 3.066 ± 0.48
4.631AspVal: 4.631 ± 0.532
1.314AspTrp: 1.314 ± 0.255
1.94AspTyr: 1.94 ± 0.33
0.0AspXaa: 0.0 ± 0.0
Glu
8.073GluAla: 8.073 ± 0.664
0.688GluCys: 0.688 ± 0.223
2.941GluAsp: 2.941 ± 0.408
2.503GluGlu: 2.503 ± 0.382
1.439GluPhe: 1.439 ± 0.299
3.63GluGly: 3.63 ± 0.44
1.815GluHis: 1.815 ± 0.353
2.628GluIle: 2.628 ± 0.405
2.691GluLys: 2.691 ± 0.485
4.819GluLeu: 4.819 ± 0.546
1.252GluMet: 1.252 ± 0.239
2.378GluAsn: 2.378 ± 0.366
3.129GluPro: 3.129 ± 0.459
2.691GluGln: 2.691 ± 0.463
3.755GluArg: 3.755 ± 0.471
3.254GluSer: 3.254 ± 0.534
2.816GluThr: 2.816 ± 0.35
4.38GluVal: 4.38 ± 0.474
1.189GluTrp: 1.189 ± 0.32
1.564GluTyr: 1.564 ± 0.305
0.0GluXaa: 0.0 ± 0.0
Phe
2.753PheAla: 2.753 ± 0.415
0.501PheCys: 0.501 ± 0.191
2.691PheAsp: 2.691 ± 0.363
1.252PheGlu: 1.252 ± 0.275
0.876PhePhe: 0.876 ± 0.229
2.816PheGly: 2.816 ± 0.393
1.064PheHis: 1.064 ± 0.229
0.751PheIle: 0.751 ± 0.2
1.064PheLys: 1.064 ± 0.267
1.439PheLeu: 1.439 ± 0.304
0.626PheMet: 0.626 ± 0.22
1.126PheAsn: 1.126 ± 0.197
1.377PhePro: 1.377 ± 0.312
1.001PheGln: 1.001 ± 0.258
1.564PheArg: 1.564 ± 0.303
1.377PheSer: 1.377 ± 0.272
2.441PheThr: 2.441 ± 0.413
1.69PheVal: 1.69 ± 0.359
0.501PheTrp: 0.501 ± 0.159
1.064PheTyr: 1.064 ± 0.211
0.0PheXaa: 0.0 ± 0.0
Gly
7.071GlyAla: 7.071 ± 1.017
0.626GlyCys: 0.626 ± 0.174
4.631GlyAsp: 4.631 ± 0.464
4.255GlyGlu: 4.255 ± 0.524
3.066GlyPhe: 3.066 ± 0.411
7.322GlyGly: 7.322 ± 0.777
1.439GlyHis: 1.439 ± 0.265
3.442GlyIle: 3.442 ± 0.499
5.569GlyLys: 5.569 ± 0.689
6.508GlyLeu: 6.508 ± 1.042
1.94GlyMet: 1.94 ± 0.283
3.191GlyAsn: 3.191 ± 0.456
3.442GlyPro: 3.442 ± 0.566
2.816GlyGln: 2.816 ± 0.511
5.131GlyArg: 5.131 ± 0.621
4.693GlySer: 4.693 ± 0.651
5.82GlyThr: 5.82 ± 0.699
5.757GlyVal: 5.757 ± 0.704
1.877GlyTrp: 1.877 ± 0.362
2.691GlyTyr: 2.691 ± 0.339
0.0GlyXaa: 0.0 ± 0.0
His
1.94HisAla: 1.94 ± 0.419
0.375HisCys: 0.375 ± 0.179
1.189HisAsp: 1.189 ± 0.275
1.564HisGlu: 1.564 ± 0.332
0.688HisPhe: 0.688 ± 0.226
1.502HisGly: 1.502 ± 0.362
1.001HisHis: 1.001 ± 0.315
1.314HisIle: 1.314 ± 0.304
0.751HisLys: 0.751 ± 0.232
2.003HisLeu: 2.003 ± 0.304
0.626HisMet: 0.626 ± 0.189
1.189HisAsn: 1.189 ± 0.305
1.314HisPro: 1.314 ± 0.317
0.876HisGln: 0.876 ± 0.234
1.252HisArg: 1.252 ± 0.357
0.939HisSer: 0.939 ± 0.271
1.189HisThr: 1.189 ± 0.228
2.253HisVal: 2.253 ± 0.4
0.313HisTrp: 0.313 ± 0.152
0.751HisTyr: 0.751 ± 0.165
0.0HisXaa: 0.0 ± 0.0
Ile
4.068IleAla: 4.068 ± 0.617
0.501IleCys: 0.501 ± 0.182
3.129IleAsp: 3.129 ± 0.428
3.191IleGlu: 3.191 ± 0.389
1.189IlePhe: 1.189 ± 0.243
3.191IleGly: 3.191 ± 0.555
0.876IleHis: 0.876 ± 0.222
1.877IleIle: 1.877 ± 0.346
1.815IleLys: 1.815 ± 0.375
3.442IleLeu: 3.442 ± 0.555
0.751IleMet: 0.751 ± 0.165
1.064IleAsn: 1.064 ± 0.212
2.816IlePro: 2.816 ± 0.293
1.752IleGln: 1.752 ± 0.281
3.004IleArg: 3.004 ± 0.462
2.879IleSer: 2.879 ± 0.416
3.817IleThr: 3.817 ± 0.497
3.317IleVal: 3.317 ± 0.462
0.25IleTrp: 0.25 ± 0.12
1.001IleTyr: 1.001 ± 0.24
0.0IleXaa: 0.0 ± 0.0
Lys
6.133LysAla: 6.133 ± 0.762
0.375LysCys: 0.375 ± 0.212
2.941LysAsp: 2.941 ± 0.463
2.628LysGlu: 2.628 ± 0.457
1.189LysPhe: 1.189 ± 0.244
2.816LysGly: 2.816 ± 0.471
1.126LysHis: 1.126 ± 0.31
2.128LysIle: 2.128 ± 0.42
2.503LysLys: 2.503 ± 0.37
3.191LysLeu: 3.191 ± 0.609
1.377LysMet: 1.377 ± 0.288
1.877LysAsn: 1.877 ± 0.346
3.004LysPro: 3.004 ± 0.412
2.378LysGln: 2.378 ± 0.401
3.129LysArg: 3.129 ± 0.445
2.378LysSer: 2.378 ± 0.434
3.254LysThr: 3.254 ± 0.4
3.066LysVal: 3.066 ± 0.404
0.563LysTrp: 0.563 ± 0.158
1.252LysTyr: 1.252 ± 0.314
0.0LysXaa: 0.0 ± 0.0
Leu
9.887LeuAla: 9.887 ± 1.035
0.688LeuCys: 0.688 ± 0.231
5.006LeuAsp: 5.006 ± 0.6
4.255LeuGlu: 4.255 ± 0.51
2.19LeuPhe: 2.19 ± 0.442
6.195LeuGly: 6.195 ± 0.676
1.502LeuHis: 1.502 ± 0.344
4.068LeuIle: 4.068 ± 0.42
4.005LeuLys: 4.005 ± 0.513
5.695LeuLeu: 5.695 ± 0.725
1.314LeuMet: 1.314 ± 0.252
2.503LeuAsn: 2.503 ± 0.39
5.945LeuPro: 5.945 ± 0.584
2.628LeuGln: 2.628 ± 0.439
5.444LeuArg: 5.444 ± 0.587
4.631LeuSer: 4.631 ± 0.596
6.008LeuThr: 6.008 ± 0.555
4.318LeuVal: 4.318 ± 0.495
1.126LeuTrp: 1.126 ± 0.234
1.627LeuTyr: 1.627 ± 0.24
0.0LeuXaa: 0.0 ± 0.0
Met
3.066MetAla: 3.066 ± 0.416
0.063MetCys: 0.063 ± 0.067
1.189MetAsp: 1.189 ± 0.237
0.939MetGlu: 0.939 ± 0.222
0.814MetPhe: 0.814 ± 0.202
1.252MetGly: 1.252 ± 0.283
0.563MetHis: 0.563 ± 0.182
0.751MetIle: 0.751 ± 0.212
1.064MetLys: 1.064 ± 0.248
1.69MetLeu: 1.69 ± 0.243
0.375MetMet: 0.375 ± 0.13
0.814MetAsn: 0.814 ± 0.236
1.502MetPro: 1.502 ± 0.336
0.814MetGln: 0.814 ± 0.269
1.439MetArg: 1.439 ± 0.294
1.69MetSer: 1.69 ± 0.282
2.441MetThr: 2.441 ± 0.387
1.314MetVal: 1.314 ± 0.269
0.25MetTrp: 0.25 ± 0.11
0.501MetTyr: 0.501 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
4.318AsnAla: 4.318 ± 0.629
0.313AsnCys: 0.313 ± 0.116
1.877AsnAsp: 1.877 ± 0.401
1.752AsnGlu: 1.752 ± 0.404
0.939AsnPhe: 0.939 ± 0.22
2.753AsnGly: 2.753 ± 0.43
0.939AsnHis: 0.939 ± 0.286
1.001AsnIle: 1.001 ± 0.242
1.189AsnLys: 1.189 ± 0.232
3.379AsnLeu: 3.379 ± 0.431
0.626AsnMet: 0.626 ± 0.191
1.001AsnAsn: 1.001 ± 0.273
2.753AsnPro: 2.753 ± 0.489
1.252AsnGln: 1.252 ± 0.301
1.69AsnArg: 1.69 ± 0.284
2.003AsnSer: 2.003 ± 0.436
2.19AsnThr: 2.19 ± 0.379
2.378AsnVal: 2.378 ± 0.358
0.688AsnTrp: 0.688 ± 0.206
0.876AsnTyr: 0.876 ± 0.198
0.0AsnXaa: 0.0 ± 0.0
Pro
6.696ProAla: 6.696 ± 0.598
0.626ProCys: 0.626 ± 0.189
4.506ProAsp: 4.506 ± 0.62
3.88ProGlu: 3.88 ± 0.664
1.815ProPhe: 1.815 ± 0.32
4.944ProGly: 4.944 ± 0.523
1.377ProHis: 1.377 ± 0.326
2.128ProIle: 2.128 ± 0.334
2.691ProLys: 2.691 ± 0.49
4.068ProLeu: 4.068 ± 0.688
1.439ProMet: 1.439 ± 0.359
1.564ProAsn: 1.564 ± 0.323
3.442ProPro: 3.442 ± 0.635
1.752ProGln: 1.752 ± 0.369
3.004ProArg: 3.004 ± 0.475
3.63ProSer: 3.63 ± 0.411
3.191ProThr: 3.191 ± 0.429
4.193ProVal: 4.193 ± 0.593
0.626ProTrp: 0.626 ± 0.228
1.126ProTyr: 1.126 ± 0.258
0.0ProXaa: 0.0 ± 0.0
Gln
4.756GlnAla: 4.756 ± 0.581
0.375GlnCys: 0.375 ± 0.165
2.19GlnAsp: 2.19 ± 0.348
1.439GlnGlu: 1.439 ± 0.38
1.064GlnPhe: 1.064 ± 0.242
2.941GlnGly: 2.941 ± 0.6
0.939GlnHis: 0.939 ± 0.245
1.815GlnIle: 1.815 ± 0.323
1.377GlnLys: 1.377 ± 0.319
3.004GlnLeu: 3.004 ± 0.361
1.064GlnMet: 1.064 ± 0.221
0.688GlnAsn: 0.688 ± 0.189
1.627GlnPro: 1.627 ± 0.35
1.377GlnGln: 1.377 ± 0.318
2.315GlnArg: 2.315 ± 0.396
2.628GlnSer: 2.628 ± 0.398
2.879GlnThr: 2.879 ± 0.429
2.315GlnVal: 2.315 ± 0.368
0.563GlnTrp: 0.563 ± 0.174
0.814GlnTyr: 0.814 ± 0.216
0.0GlnXaa: 0.0 ± 0.0
Arg
6.008ArgAla: 6.008 ± 0.551
0.939ArgCys: 0.939 ± 0.231
3.88ArgAsp: 3.88 ± 0.543
3.692ArgGlu: 3.692 ± 0.633
1.94ArgPhe: 1.94 ± 0.337
5.257ArgGly: 5.257 ± 0.613
1.752ArgHis: 1.752 ± 0.434
3.692ArgIle: 3.692 ± 0.505
2.691ArgLys: 2.691 ± 0.369
5.319ArgLeu: 5.319 ± 0.473
1.627ArgMet: 1.627 ± 0.302
2.378ArgAsn: 2.378 ± 0.373
2.879ArgPro: 2.879 ± 0.444
1.252ArgGln: 1.252 ± 0.273
5.069ArgArg: 5.069 ± 0.814
3.254ArgSer: 3.254 ± 0.519
3.442ArgThr: 3.442 ± 0.438
4.38ArgVal: 4.38 ± 0.471
1.627ArgTrp: 1.627 ± 0.3
1.94ArgTyr: 1.94 ± 0.372
0.0ArgXaa: 0.0 ± 0.0
Ser
6.383SerAla: 6.383 ± 0.811
0.25SerCys: 0.25 ± 0.125
3.129SerAsp: 3.129 ± 0.387
3.379SerGlu: 3.379 ± 0.335
1.877SerPhe: 1.877 ± 0.278
5.82SerGly: 5.82 ± 0.705
1.126SerHis: 1.126 ± 0.24
3.442SerIle: 3.442 ± 0.469
2.315SerLys: 2.315 ± 0.318
4.631SerLeu: 4.631 ± 0.458
1.064SerMet: 1.064 ± 0.235
1.377SerAsn: 1.377 ± 0.236
2.503SerPro: 2.503 ± 0.354
2.378SerGln: 2.378 ± 0.344
3.317SerArg: 3.317 ± 0.481
2.566SerSer: 2.566 ± 0.491
3.191SerThr: 3.191 ± 0.456
4.38SerVal: 4.38 ± 0.589
1.189SerTrp: 1.189 ± 0.303
1.564SerTyr: 1.564 ± 0.277
0.0SerXaa: 0.0 ± 0.0
Thr
8.886ThrAla: 8.886 ± 0.712
0.688ThrCys: 0.688 ± 0.23
3.755ThrAsp: 3.755 ± 0.42
3.942ThrGlu: 3.942 ± 0.537
2.065ThrPhe: 2.065 ± 0.275
5.006ThrGly: 5.006 ± 0.58
1.126ThrHis: 1.126 ± 0.255
2.879ThrIle: 2.879 ± 0.431
3.004ThrLys: 3.004 ± 0.395
5.006ThrLeu: 5.006 ± 0.502
1.189ThrMet: 1.189 ± 0.279
2.628ThrAsn: 2.628 ± 0.42
4.068ThrPro: 4.068 ± 0.508
2.128ThrGln: 2.128 ± 0.323
2.941ThrArg: 2.941 ± 0.425
3.254ThrSer: 3.254 ± 0.439
3.692ThrThr: 3.692 ± 0.445
5.757ThrVal: 5.757 ± 0.824
0.939ThrTrp: 0.939 ± 0.294
2.753ThrTyr: 2.753 ± 0.372
0.0ThrXaa: 0.0 ± 0.0
Val
7.885ValAla: 7.885 ± 0.79
0.563ValCys: 0.563 ± 0.151
4.693ValAsp: 4.693 ± 0.642
4.38ValGlu: 4.38 ± 0.507
1.627ValPhe: 1.627 ± 0.266
4.38ValGly: 4.38 ± 0.51
1.314ValHis: 1.314 ± 0.327
2.315ValIle: 2.315 ± 0.403
3.442ValLys: 3.442 ± 0.394
5.82ValLeu: 5.82 ± 0.511
1.877ValMet: 1.877 ± 0.307
2.253ValAsn: 2.253 ± 0.313
4.255ValPro: 4.255 ± 0.486
2.753ValGln: 2.753 ± 0.343
5.006ValArg: 5.006 ± 0.513
4.318ValSer: 4.318 ± 0.516
5.257ValThr: 5.257 ± 0.513
4.443ValVal: 4.443 ± 0.491
1.001ValTrp: 1.001 ± 0.197
2.003ValTyr: 2.003 ± 0.353
0.0ValXaa: 0.0 ± 0.0
Trp
2.628TrpAla: 2.628 ± 0.442
0.25TrpCys: 0.25 ± 0.12
1.564TrpAsp: 1.564 ± 0.359
0.626TrpGlu: 0.626 ± 0.198
0.375TrpPhe: 0.375 ± 0.155
1.001TrpGly: 1.001 ± 0.278
0.501TrpHis: 0.501 ± 0.204
0.626TrpIle: 0.626 ± 0.181
0.375TrpLys: 0.375 ± 0.127
1.877TrpLeu: 1.877 ± 0.319
0.063TrpMet: 0.063 ± 0.054
0.751TrpAsn: 0.751 ± 0.254
0.563TrpPro: 0.563 ± 0.181
0.563TrpGln: 0.563 ± 0.159
1.502TrpArg: 1.502 ± 0.252
1.126TrpSer: 1.126 ± 0.238
1.314TrpThr: 1.314 ± 0.263
1.064TrpVal: 1.064 ± 0.283
0.438TrpTrp: 0.438 ± 0.176
0.188TrpTyr: 0.188 ± 0.092
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.19TyrAla: 2.19 ± 0.32
0.313TyrCys: 0.313 ± 0.139
1.564TyrAsp: 1.564 ± 0.296
2.065TyrGlu: 2.065 ± 0.311
0.814TyrPhe: 0.814 ± 0.222
2.065TyrGly: 2.065 ± 0.346
0.313TyrHis: 0.313 ± 0.147
1.064TyrIle: 1.064 ± 0.205
1.439TyrLys: 1.439 ± 0.231
2.19TyrLeu: 2.19 ± 0.411
0.563TyrMet: 0.563 ± 0.183
1.189TyrAsn: 1.189 ± 0.255
1.001TyrPro: 1.001 ± 0.224
1.189TyrGln: 1.189 ± 0.272
2.065TyrArg: 2.065 ± 0.275
1.439TyrSer: 1.439 ± 0.33
2.253TyrThr: 2.253 ± 0.376
1.752TyrVal: 1.752 ± 0.311
0.188TyrTrp: 0.188 ± 0.097
0.563TyrTyr: 0.563 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (15981 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski