Oricola cellulosilytica
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3475 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R0PHY5|A0A4R0PHY5_9RHIZ Uncharacterized protein OS=Oricola cellulosilytica OX=1429082 GN=E0D97_01665 PE=4 SV=1
MM1 pKa = 8.28 RR2 pKa = 11.84 IRR4 pKa = 11.84 NIALAATIGSLTALNGAVAQDD25 pKa = 3.56 TLIEE29 pKa = 4.06 RR30 pKa = 11.84 VEE32 pKa = 4.26 IGVLDD37 pKa = 4.04 CVVSGGTGYY46 pKa = 10.56 IFGSTKK52 pKa = 10.71 DD53 pKa = 3.21 VGCTYY58 pKa = 10.61 KK59 pKa = 10.61 PADD62 pKa = 3.81 GAPEE66 pKa = 3.73 EE67 pKa = 4.82 TYY69 pKa = 10.59 FGVISKK75 pKa = 10.52 YY76 pKa = 10.73 GVDD79 pKa = 3.09 IGTTEE84 pKa = 3.84 ASYY87 pKa = 7.58 MTWAVLAPTDD97 pKa = 3.78 DD98 pKa = 4.22 PYY100 pKa = 11.54 RR101 pKa = 11.84 PGALAGDD108 pKa = 4.03 YY109 pKa = 10.7 FGVSAEE115 pKa = 4.11 ATVGAGIGANALLGGSDD132 pKa = 3.63 EE133 pKa = 4.8 TIALQPLSVSGQTGLNFALGVSEE156 pKa = 5.26 LEE158 pKa = 4.3 LRR160 pKa = 11.84 SSADD164 pKa = 2.98
Molecular weight: 16.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.813
IPC2_protein 4.05
IPC_protein 3.986
Toseland 3.783
ProMoST 4.062
Dawson 3.961
Bjellqvist 4.164
Wikipedia 3.872
Rodwell 3.808
Grimsley 3.694
Solomon 3.948
Lehninger 3.897
Nozaki 4.075
DTASelect 4.266
Thurlkill 3.834
EMBOSS 3.884
Sillero 4.101
Patrickios 1.926
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 4.011
Protein with the highest isoelectric point:
>tr|A0A4R0PK49|A0A4R0PK49_9RHIZ Pantothenate kinase OS=Oricola cellulosilytica OX=1429082 GN=coaA PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 8.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATPGGRR28 pKa = 11.84 KK29 pKa = 9.06 VLATRR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.01 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3475
0
3475
1108791
31
3243
319.1
34.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.977 ± 0.053
0.84 ± 0.012
5.948 ± 0.035
6.251 ± 0.04
3.927 ± 0.027
8.64 ± 0.038
2.04 ± 0.019
5.487 ± 0.032
3.349 ± 0.03
9.569 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.625 ± 0.02
2.769 ± 0.02
4.881 ± 0.027
2.782 ± 0.019
7.068 ± 0.041
5.602 ± 0.028
5.295 ± 0.027
7.447 ± 0.033
1.276 ± 0.018
2.229 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here