Streptococcus phage Javan414

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B6Q6|A0A4D6B6Q6_9CAUD Capsid and scaffold protein OS=Streptococcus phage Javan414 OX=2548155 GN=Javan414_0005 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.1KK3 pKa = 10.19KK4 pKa = 10.79NEE6 pKa = 3.93GAVRR10 pKa = 11.84NLIALCLITLAVVLGIAAFGAVVKK34 pKa = 10.25EE35 pKa = 4.03KK36 pKa = 10.62QSEE39 pKa = 4.26NKK41 pKa = 9.69IVTSSSSNVDD51 pKa = 3.36DD52 pKa = 4.51SDD54 pKa = 5.04DD55 pKa = 3.84YY56 pKa = 11.98SSEE59 pKa = 4.11DD60 pKa = 3.69DD61 pKa = 3.31NDD63 pKa = 3.73YY64 pKa = 11.29NYY66 pKa = 11.19SSSSSSNYY74 pKa = 9.56SSSSSSEE81 pKa = 3.39IFDD84 pKa = 4.12PNNYY88 pKa = 8.41EE89 pKa = 4.19VPDD92 pKa = 4.14FATWNHH98 pKa = 6.36DD99 pKa = 3.55QLEE102 pKa = 4.41HH103 pKa = 6.93DD104 pKa = 4.86KK105 pKa = 10.79KK106 pKa = 10.72IQITGTVVQNMEE118 pKa = 4.53DD119 pKa = 3.35DD120 pKa = 4.58GIYY123 pKa = 9.71YY124 pKa = 10.34LRR126 pKa = 11.84VAMDD130 pKa = 4.22DD131 pKa = 4.49DD132 pKa = 4.25YY133 pKa = 12.07DD134 pKa = 5.58KK135 pKa = 11.27IVMVGISKK143 pKa = 10.24YY144 pKa = 11.04VYY146 pKa = 10.19DD147 pKa = 5.18DD148 pKa = 4.88VIAEE152 pKa = 4.25DD153 pKa = 4.82DD154 pKa = 3.66NVTFYY159 pKa = 11.37GVAKK163 pKa = 10.45GLTSYY168 pKa = 11.09EE169 pKa = 4.08STLGKK174 pKa = 8.53TVTLPLMYY182 pKa = 10.42GHH184 pKa = 7.09YY185 pKa = 10.85YY186 pKa = 10.3NINSYY191 pKa = 11.39GNN193 pKa = 3.34

Molecular weight: 21.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B6V3|A0A4D6B6V3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan414 OX=2548155 GN=Javan414_0039 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.17RR3 pKa = 11.84NKK5 pKa = 8.81TKK7 pKa = 10.32YY8 pKa = 9.53PSIFTYY14 pKa = 7.28EE15 pKa = 4.01TKK17 pKa = 10.15KK18 pKa = 10.43GKK20 pKa = 9.9RR21 pKa = 11.84YY22 pKa = 8.44YY23 pKa = 10.58VRR25 pKa = 11.84RR26 pKa = 11.84KK27 pKa = 9.71FKK29 pKa = 10.87LHH31 pKa = 6.28GKK33 pKa = 6.06QTEE36 pKa = 4.03ASASGLKK43 pKa = 9.01TLAEE47 pKa = 4.14ARR49 pKa = 11.84QALAEE54 pKa = 4.15IEE56 pKa = 4.27NKK58 pKa = 9.36IANGDD63 pKa = 3.46YY64 pKa = 10.76DD65 pKa = 3.63PRR67 pKa = 11.84KK68 pKa = 10.68NMTVDD73 pKa = 4.89DD74 pKa = 3.9YY75 pKa = 11.29WQIYY79 pKa = 8.63SDD81 pKa = 3.58NRR83 pKa = 11.84IKK85 pKa = 9.93TGRR88 pKa = 11.84WAPDD92 pKa = 3.78TIATKK97 pKa = 9.77MSWYY101 pKa = 9.83KK102 pKa = 10.36YY103 pKa = 9.51HH104 pKa = 6.28FHH106 pKa = 6.35KK107 pKa = 10.95QFGRR111 pKa = 11.84TLLKK115 pKa = 10.41DD116 pKa = 3.57IKK118 pKa = 8.48RR119 pKa = 11.84TKK121 pKa = 9.6YY122 pKa = 8.42EE123 pKa = 3.99AYY125 pKa = 9.88VSSMLKK131 pKa = 9.97NYY133 pKa = 10.31SRR135 pKa = 11.84TSVIQISALFEE146 pKa = 4.44SMLTDD151 pKa = 4.23AVINDD156 pKa = 4.49YY157 pKa = 10.76IDD159 pKa = 3.63KK160 pKa = 10.99NPILRR165 pKa = 11.84IYY167 pKa = 9.63IGKK170 pKa = 9.76SDD172 pKa = 3.65IPAKK176 pKa = 10.27KK177 pKa = 9.74KK178 pKa = 10.44RR179 pKa = 11.84LSLEE183 pKa = 4.32DD184 pKa = 3.46FRR186 pKa = 11.84IWDD189 pKa = 3.36KK190 pKa = 10.74CARR193 pKa = 11.84EE194 pKa = 4.01ILSSYY199 pKa = 10.11DD200 pKa = 3.22YY201 pKa = 11.51TMVRR205 pKa = 11.84LTYY208 pKa = 9.91FGLRR212 pKa = 11.84RR213 pKa = 11.84SEE215 pKa = 3.92VLGVKK220 pKa = 10.42FGSLSLVDD228 pKa = 3.7GRR230 pKa = 11.84FKK232 pKa = 10.84IFLDD236 pKa = 3.56EE237 pKa = 4.3SRR239 pKa = 11.84TSRR242 pKa = 11.84RR243 pKa = 11.84PEE245 pKa = 3.81GGRR248 pKa = 11.84MKK250 pKa = 10.4TKK252 pKa = 9.25TSEE255 pKa = 3.98RR256 pKa = 11.84YY257 pKa = 9.47VLVDD261 pKa = 3.39EE262 pKa = 4.84EE263 pKa = 4.68TTEE266 pKa = 4.24LLQKK270 pKa = 11.28AMILSRR276 pKa = 11.84QIAKK280 pKa = 7.27EE281 pKa = 3.93TNRR284 pKa = 11.84ILGQGDD290 pKa = 4.81FIFVDD295 pKa = 3.37TGMKK299 pKa = 9.74IKK301 pKa = 10.11NHH303 pKa = 6.29KK304 pKa = 9.37GNPIGYY310 pKa = 8.16SHH312 pKa = 7.66IAYY315 pKa = 9.9AFRR318 pKa = 11.84KK319 pKa = 10.27VSDD322 pKa = 3.24KK323 pKa = 10.96TNIYY327 pKa = 7.62VTPHH331 pKa = 4.66TMRR334 pKa = 11.84HH335 pKa = 5.08FFATQGQIAGVPVEE349 pKa = 4.8HH350 pKa = 6.66MAAALGHH357 pKa = 5.31STSYY361 pKa = 7.77MTQKK365 pKa = 8.95YY366 pKa = 6.41THH368 pKa = 6.9IKK370 pKa = 10.67DD371 pKa = 3.64EE372 pKa = 4.38VASEE376 pKa = 4.27VTDD379 pKa = 3.47SFLRR383 pKa = 11.84AIKK386 pKa = 10.4

Molecular weight: 44.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

10746

52

1347

238.8

26.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.314 ± 0.698

0.614 ± 0.128

6.57 ± 0.299

7.51 ± 0.511

4.029 ± 0.312

6.635 ± 0.34

1.126 ± 0.128

6.458 ± 0.341

8.217 ± 0.374

7.854 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.466 ± 0.227

5.667 ± 0.264

2.671 ± 0.142

3.732 ± 0.308

3.797 ± 0.289

6.309 ± 0.406

7.398 ± 0.577

6.235 ± 0.251

1.405 ± 0.18

3.992 ± 0.372
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski