Parvularcula bermudensis (strain ATCC BAA-594 / HTCC2503 / KCTC 12087)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Parvularculales; Parvularculaceae; Parvularcula; Parvularcula bermudensis

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0TEQ1|E0TEQ1_PARBH Transcription termination/antitermination protein NusA OS=Parvularcula bermudensis (strain ATCC BAA-594 / HTCC2503 / KCTC 12087) OX=314260 GN=nusA PE=3 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84LSTGLFAASSLIALAACSSTGGTPDD27 pKa = 5.33PILGEE32 pKa = 4.76DD33 pKa = 4.12GQPAPPRR40 pKa = 11.84NLVTNALSYY49 pKa = 10.28RR50 pKa = 11.84AEE52 pKa = 4.03NHH54 pKa = 5.62GSAVSEE60 pKa = 4.42TYY62 pKa = 10.97DD63 pKa = 3.7LAVTAMRR70 pKa = 11.84TRR72 pKa = 11.84YY73 pKa = 6.24QTNGAAVFVGIEE85 pKa = 3.8HH86 pKa = 7.07DD87 pKa = 3.69AVGEE91 pKa = 4.28SEE93 pKa = 4.36TTEE96 pKa = 3.87FRR98 pKa = 11.84YY99 pKa = 10.3DD100 pKa = 3.09AAADD104 pKa = 3.78TFTIDD109 pKa = 4.23IDD111 pKa = 4.74LPNADD116 pKa = 4.98DD117 pKa = 5.58APILVNEE124 pKa = 4.23TWGPLLLLTPKK135 pKa = 10.75DD136 pKa = 3.74LFGLNNGVSAVYY148 pKa = 9.55LAKK151 pKa = 10.68QPDD154 pKa = 4.1FYY156 pKa = 11.73GFVDD160 pKa = 3.56EE161 pKa = 4.88TLIGGNEE168 pKa = 4.0FTADD172 pKa = 3.52AYY174 pKa = 10.08IDD176 pKa = 3.55ALGAVDD182 pKa = 3.73ATGSGSGYY190 pKa = 9.52LALWVTIANDD200 pKa = 3.56LGFEE204 pKa = 4.08GSEE207 pKa = 3.9RR208 pKa = 11.84DD209 pKa = 3.84VGSLPIYY216 pKa = 9.64IANDD220 pKa = 3.49PATFGAPGSLAGDD233 pKa = 3.82IEE235 pKa = 4.25AATAYY240 pKa = 10.91VEE242 pKa = 4.44DD243 pKa = 4.98LSPAAFSDD251 pKa = 3.72AQAAAPVPSSAFDD264 pKa = 4.54PIMSEE269 pKa = 4.4LLATAPEE276 pKa = 4.32SFGVNVEE283 pKa = 4.18GQISDD288 pKa = 3.56NDD290 pKa = 3.37ASFIIAVMDD299 pKa = 4.17DD300 pKa = 3.45VGGTAVEE307 pKa = 4.68DD308 pKa = 4.38YY309 pKa = 9.36ITAITIITDD318 pKa = 4.36DD319 pKa = 3.92IEE321 pKa = 4.2AAPNYY326 pKa = 9.83LYY328 pKa = 10.36QANGVTYY335 pKa = 9.48YY336 pKa = 10.42QYY338 pKa = 9.66KK339 pKa = 9.07TEE341 pKa = 4.14GDD343 pKa = 3.82VVDD346 pKa = 4.1TRR348 pKa = 11.84FVAAGEE354 pKa = 4.18WQKK357 pKa = 9.76TEE359 pKa = 3.91ASGEE363 pKa = 4.33TIFGHH368 pKa = 6.0FVYY371 pKa = 10.18GQLTDD376 pKa = 4.02PDD378 pKa = 4.05EE379 pKa = 4.64MPTSGTASYY388 pKa = 9.61TGTVYY393 pKa = 11.04GSILRR398 pKa = 11.84QNRR401 pKa = 11.84VDD403 pKa = 3.62SLRR406 pKa = 11.84GGLNLNTDD414 pKa = 4.8FQTGALDD421 pKa = 3.38MTFNADD427 pKa = 2.46IAYY430 pKa = 9.98RR431 pKa = 11.84DD432 pKa = 4.22DD433 pKa = 3.76EE434 pKa = 5.57GITQFIDD441 pKa = 3.43YY442 pKa = 10.96ADD444 pKa = 3.6FTGTGRR450 pKa = 11.84IDD452 pKa = 3.59DD453 pKa = 4.45ANFDD457 pKa = 3.63GTMQGVTSIDD467 pKa = 3.29TSEE470 pKa = 4.06TDD472 pKa = 2.97SPLVVDD478 pKa = 5.73GSFEE482 pKa = 3.98GHH484 pKa = 6.52FYY486 pKa = 11.28GPTAQEE492 pKa = 3.71AGGTFEE498 pKa = 5.12FSNTEE503 pKa = 3.56AAATGSFVAVRR514 pKa = 11.84DD515 pKa = 3.73

Molecular weight: 54.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E0TGT2|E0TGT2_PARBH Anthranilate phosphoribosyltransferase OS=Parvularcula bermudensis (strain ATCC BAA-594 / HTCC2503 / KCTC 12087) OX=314260 GN=trpD PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVLKK11 pKa = 10.35RR12 pKa = 11.84KK13 pKa = 9.12RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.52GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.68ATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.53GRR39 pKa = 11.84KK40 pKa = 8.77RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2687

0

2687

870527

17

2831

324.0

35.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.18 ± 0.064

0.759 ± 0.014

6.294 ± 0.04

6.28 ± 0.04

3.74 ± 0.033

8.485 ± 0.043

1.891 ± 0.024

5.124 ± 0.031

3.03 ± 0.044

10.339 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.022

2.361 ± 0.026

5.268 ± 0.033

3.136 ± 0.024

7.138 ± 0.048

5.618 ± 0.03

5.641 ± 0.032

7.011 ± 0.041

1.205 ± 0.019

2.292 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski