Poseidonocella sedimentorum
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3242 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I6DGX3|A0A1I6DGX3_9RHOB Peptidyl-prolyl cis-trans isomerase OS=Poseidonocella sedimentorum OX=871652 GN=SAMN04515673_103218 PE=3 SV=1
MM1 pKa = 7.16 NRR3 pKa = 11.84 IFVSGIALAASAGMASAGGIDD24 pKa = 4.7 RR25 pKa = 11.84 SGQSIGLIFEE35 pKa = 4.59 EE36 pKa = 4.72 GNYY39 pKa = 10.19 AQLSFGSVSPDD50 pKa = 2.95 VSGAQVIAVPTLAGVIPAGSEE71 pKa = 3.93 SGNMSGSYY79 pKa = 8.17 TQFSLGFKK87 pKa = 9.41 QAYY90 pKa = 10.15 DD91 pKa = 3.57 NGLEE95 pKa = 3.99 MAFILDD101 pKa = 3.73 QPFGADD107 pKa = 3.01 VEE109 pKa = 4.58 YY110 pKa = 10.35 PDD112 pKa = 5.61 DD113 pKa = 3.71 EE114 pKa = 5.15 TYY116 pKa = 10.59 FAAGSTAEE124 pKa = 4.36 LSSTAITGIVKK135 pKa = 10.39 YY136 pKa = 10.29 SLPNNVSVFGGLRR149 pKa = 11.84 YY150 pKa = 9.7 QSLEE154 pKa = 3.71 AKK156 pKa = 10.21 AYY158 pKa = 9.54 IPFVTASAGPTAGVPYY174 pKa = 10.19 EE175 pKa = 4.09 VDD177 pKa = 3.69 GEE179 pKa = 4.27 KK180 pKa = 10.64 DD181 pKa = 2.74 WGLGYY186 pKa = 10.91 LLGVAYY192 pKa = 8.29 EE193 pKa = 4.42 RR194 pKa = 11.84 PDD196 pKa = 2.94 IALRR200 pKa = 11.84 VALTYY205 pKa = 11.05 NSAIDD210 pKa = 4.1 HH211 pKa = 6.72 EE212 pKa = 4.86 IDD214 pKa = 3.36 TYY216 pKa = 9.88 EE217 pKa = 4.17 TSALNPGGLDD227 pKa = 3.12 TVTDD231 pKa = 3.64 VSTPQSLNLEE241 pKa = 4.28 FQSGIAADD249 pKa = 3.76 TLLFGSVRR257 pKa = 11.84 WVDD260 pKa = 2.94 WSEE263 pKa = 4.06 FDD265 pKa = 5.73 ISPQDD270 pKa = 3.43 YY271 pKa = 10.09 DD272 pKa = 3.65 ALLDD276 pKa = 4.07 FSRR279 pKa = 11.84 GAGDD283 pKa = 3.72 YY284 pKa = 11.23 SLVSYY289 pKa = 11.32 DD290 pKa = 5.71 DD291 pKa = 4.03 DD292 pKa = 3.91 TTSYY296 pKa = 11.62 SLGLGRR302 pKa = 11.84 RR303 pKa = 11.84 INEE306 pKa = 3.84 TWSAAVTVGYY316 pKa = 8.24 EE317 pKa = 3.75 ASAGGFASNLGPTDD331 pKa = 2.96 GRR333 pKa = 11.84 TSIGLAATYY342 pKa = 10.2 TDD344 pKa = 3.94 GPMKK348 pKa = 9.81 ITGGVSYY355 pKa = 10.97 VRR357 pKa = 11.84 IGDD360 pKa = 3.96 AEE362 pKa = 4.31 TTLDD366 pKa = 3.6 GTSQATEE373 pKa = 3.88 FDD375 pKa = 4.43 DD376 pKa = 4.63 NSAIGWGLRR385 pKa = 11.84 IGYY388 pKa = 9.77 AFF390 pKa = 4.33
Molecular weight: 40.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.808
IPC_protein 3.821
Toseland 3.605
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.643
Grimsley 3.516
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.164
Thurlkill 3.656
EMBOSS 3.757
Sillero 3.948
Patrickios 0.795
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.826
Protein with the highest isoelectric point:
>tr|A0A1I6DHP4|A0A1I6DHP4_9RHOB Uncharacterized protein OS=Poseidonocella sedimentorum OX=871652 GN=SAMN04515673_103276 PE=4 SV=1
MM1 pKa = 7.09 TRR3 pKa = 11.84 YY4 pKa = 9.62 PPTAWRR10 pKa = 11.84 ALRR13 pKa = 11.84 PVKK16 pKa = 9.15 TARR19 pKa = 11.84 ALAVLAALCLAAPAWAAGAKK39 pKa = 10.0 KK40 pKa = 10.05 PEE42 pKa = 4.38 APAGPVNSTPACQVMDD58 pKa = 5.3 KK59 pKa = 10.68 ILALDD64 pKa = 4.01 PLSHH68 pKa = 6.42 KK69 pKa = 10.41 VAVGIDD75 pKa = 3.47 HH76 pKa = 6.85 FQLIDD81 pKa = 3.29 QYY83 pKa = 9.84 RR84 pKa = 11.84 TKK86 pKa = 10.52 IEE88 pKa = 3.75 IARR91 pKa = 11.84 QSLRR95 pKa = 11.84 ALPASVSVPDD105 pKa = 3.66 RR106 pKa = 11.84 EE107 pKa = 3.97 RR108 pKa = 11.84 LQDD111 pKa = 3.62 HH112 pKa = 5.46 VTAHH116 pKa = 6.97 ADD118 pKa = 3.32 VAEE121 pKa = 4.37 TVAINGSSPAAALITSEE138 pKa = 4.08 PFLRR142 pKa = 11.84 RR143 pKa = 11.84 ALQVEE148 pKa = 4.43 ADD150 pKa = 4.06 HH151 pKa = 6.99 KK152 pKa = 11.46 ALGCLSPSWRR162 pKa = 11.84 DD163 pKa = 3.1 RR164 pKa = 11.84 LSFLTDD170 pKa = 3.54 LFKK173 pKa = 10.58 PVQRR177 pKa = 11.84 IWAHH181 pKa = 4.32 EE182 pKa = 3.92 RR183 pKa = 11.84 VRR185 pKa = 11.84 PFILWGICFSLVVWARR201 pKa = 11.84 RR202 pKa = 11.84 RR203 pKa = 11.84 QKK205 pKa = 10.5 RR206 pKa = 11.84 YY207 pKa = 8.76 QALNALPDD215 pKa = 3.56 YY216 pKa = 10.3 DD217 pKa = 4.24 ARR219 pKa = 11.84 EE220 pKa = 4.18 APRR223 pKa = 11.84 VNCSLPAQVHH233 pKa = 5.84 IEE235 pKa = 3.81 ADD237 pKa = 4.41 GIPIRR242 pKa = 11.84 ILDD245 pKa = 3.78 ISRR248 pKa = 11.84 KK249 pKa = 7.11 GARR252 pKa = 11.84 FALSRR257 pKa = 11.84 PVEE260 pKa = 4.43 LPDD263 pKa = 4.73 EE264 pKa = 4.11 IHH266 pKa = 6.47 IALPAEE272 pKa = 4.17 IRR274 pKa = 11.84 RR275 pKa = 11.84 TALLRR280 pKa = 11.84 RR281 pKa = 11.84 MRR283 pKa = 11.84 ARR285 pKa = 11.84 HH286 pKa = 5.24 GAVMFLPRR294 pKa = 11.84 LTDD297 pKa = 3.3 VEE299 pKa = 4.22 FAKK302 pKa = 10.68 VLRR305 pKa = 11.84 HH306 pKa = 6.01 SDD308 pKa = 3.54 LPVASRR314 pKa = 11.84 ALPPAKK320 pKa = 9.69 PPRR323 pKa = 11.84 GAALAGLLGWLPQGRR338 pKa = 11.84 RR339 pKa = 11.84 SKK341 pKa = 11.22 AA342 pKa = 3.05
Molecular weight: 37.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.531
IPC_protein 10.438
Toseland 10.804
ProMoST 10.584
Dawson 10.862
Bjellqvist 10.613
Wikipedia 11.111
Rodwell 10.965
Grimsley 10.891
Solomon 11.052
Lehninger 11.008
Nozaki 10.789
DTASelect 10.599
Thurlkill 10.789
EMBOSS 11.213
Sillero 10.804
Patrickios 10.672
IPC_peptide 11.052
IPC2_peptide 9.78
IPC2.peptide.svr19 8.771
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3242
0
3242
1066642
30
17790
329.0
35.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.109 ± 0.071
0.84 ± 0.02
6.017 ± 0.066
6.197 ± 0.042
3.671 ± 0.033
9.017 ± 0.083
1.949 ± 0.029
5.087 ± 0.03
2.686 ± 0.05
10.331 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.545 ± 0.037
2.285 ± 0.023
5.228 ± 0.059
2.908 ± 0.023
7.073 ± 0.072
5.199 ± 0.056
5.425 ± 0.087
6.989 ± 0.034
1.324 ± 0.019
2.12 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here