Streptococcus phage Javan39

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AAN6|A0A4D6AAN6_9CAUD Terminase large subunit OS=Streptococcus phage Javan39 OX=2548144 GN=Javan39_0027 PE=4 SV=1
MM1 pKa = 7.42LVYY4 pKa = 9.63PAIFTQDD11 pKa = 2.26SDD13 pKa = 4.73YY14 pKa = 11.74IMVTFPDD21 pKa = 3.7VPEE24 pKa = 5.63AITQGEE30 pKa = 4.54DD31 pKa = 3.17FQEE34 pKa = 4.6AYY36 pKa = 10.36EE37 pKa = 4.17MAVEE41 pKa = 4.15VLGFALEE48 pKa = 5.25DD49 pKa = 3.43YY50 pKa = 10.49TDD52 pKa = 3.75YY53 pKa = 11.24PKK55 pKa = 10.97ASSVSDD61 pKa = 3.76LKK63 pKa = 10.96EE64 pKa = 3.7QYY66 pKa = 10.23LGSDD70 pKa = 3.04IALIGIDD77 pKa = 3.72MIAYY81 pKa = 6.12MKK83 pKa = 10.17KK84 pKa = 8.24YY85 pKa = 9.68HH86 pKa = 5.89SKK88 pKa = 10.45KK89 pKa = 8.6VRR91 pKa = 11.84KK92 pKa = 9.32NVTIPEE98 pKa = 4.25WLNNAAEE105 pKa = 4.35DD106 pKa = 3.85KK107 pKa = 10.81NLNFSQVLTEE117 pKa = 4.09ALEE120 pKa = 4.63LKK122 pKa = 10.44LQAA125 pKa = 4.95

Molecular weight: 14.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A9J7|A0A4D6A9J7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan39 OX=2548144 GN=Javan39_0061 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.15NGPKK7 pKa = 10.23GGGRR11 pKa = 11.84IGAVKK16 pKa = 10.31GRR18 pKa = 11.84SQSHH22 pKa = 5.0NPKK25 pKa = 9.14TGLYY29 pKa = 7.7TKK31 pKa = 10.29RR32 pKa = 11.84DD33 pKa = 3.38TSTGKK38 pKa = 10.62FMDD41 pKa = 3.68TKK43 pKa = 8.85TTGGKK48 pKa = 9.61FKK50 pKa = 10.73GVRR53 pKa = 11.84TEE55 pKa = 3.81KK56 pKa = 10.97

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12680

39

1313

186.5

21.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.483 ± 0.525

0.473 ± 0.091

6.569 ± 0.301

7.074 ± 0.429

4.101 ± 0.28

6.23 ± 0.42

1.222 ± 0.127

7.011 ± 0.303

9.377 ± 0.469

8.107 ± 0.323

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.174

5.931 ± 0.254

2.784 ± 0.211

3.927 ± 0.231

3.746 ± 0.189

6.546 ± 0.299

6.467 ± 0.364

6.341 ± 0.22

1.191 ± 0.101

4.156 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski