Amino acid dipepetide frequency for Streptococcus phage Javan39

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.155AlaAla: 3.155 ± 1.286
0.315AlaCys: 0.315 ± 0.147
4.18AlaAsp: 4.18 ± 0.672
5.284AlaGlu: 5.284 ± 0.771
2.13AlaPhe: 2.13 ± 0.396
3.865AlaGly: 3.865 ± 0.724
0.631AlaHis: 0.631 ± 0.187
5.442AlaIle: 5.442 ± 0.55
6.073AlaLys: 6.073 ± 0.719
4.969AlaLeu: 4.969 ± 0.78
1.262AlaMet: 1.262 ± 0.374
3.549AlaAsn: 3.549 ± 0.662
1.577AlaPro: 1.577 ± 0.347
2.76AlaGln: 2.76 ± 0.373
2.603AlaArg: 2.603 ± 0.536
4.574AlaSer: 4.574 ± 0.711
4.574AlaThr: 4.574 ± 0.574
4.259AlaVal: 4.259 ± 0.557
0.868AlaTrp: 0.868 ± 0.362
2.76AlaTyr: 2.76 ± 0.489
0.0AlaXaa: 0.0 ± 0.0
Cys
0.237CysAla: 0.237 ± 0.124
0.079CysCys: 0.079 ± 0.08
0.394CysAsp: 0.394 ± 0.188
0.237CysGlu: 0.237 ± 0.128
0.394CysPhe: 0.394 ± 0.163
0.315CysGly: 0.315 ± 0.186
0.079CysHis: 0.079 ± 0.074
0.237CysIle: 0.237 ± 0.154
0.473CysLys: 0.473 ± 0.222
0.315CysLeu: 0.315 ± 0.159
0.158CysMet: 0.158 ± 0.137
0.237CysAsn: 0.237 ± 0.116
0.158CysPro: 0.158 ± 0.096
0.079CysGln: 0.079 ± 0.079
0.158CysArg: 0.158 ± 0.101
0.079CysSer: 0.079 ± 0.073
0.315CysThr: 0.315 ± 0.15
0.394CysVal: 0.394 ± 0.166
0.158CysTrp: 0.158 ± 0.124
0.237CysTyr: 0.237 ± 0.152
0.0CysXaa: 0.0 ± 0.0
Asp
2.839AspAla: 2.839 ± 0.462
0.158AspCys: 0.158 ± 0.106
4.653AspAsp: 4.653 ± 0.688
5.284AspGlu: 5.284 ± 0.579
3.786AspPhe: 3.786 ± 0.55
4.574AspGly: 4.574 ± 0.866
0.552AspHis: 0.552 ± 0.185
4.574AspIle: 4.574 ± 0.651
6.467AspLys: 6.467 ± 0.694
5.442AspLeu: 5.442 ± 0.654
1.577AspMet: 1.577 ± 0.287
4.653AspAsn: 4.653 ± 0.546
1.972AspPro: 1.972 ± 0.377
1.341AspGln: 1.341 ± 0.335
2.208AspArg: 2.208 ± 0.362
4.338AspSer: 4.338 ± 0.678
3.628AspThr: 3.628 ± 0.675
3.155AspVal: 3.155 ± 0.497
0.71AspTrp: 0.71 ± 0.321
3.786AspTyr: 3.786 ± 0.604
0.0AspXaa: 0.0 ± 0.0
Glu
4.653GluAla: 4.653 ± 0.821
0.079GluCys: 0.079 ± 0.094
3.865GluAsp: 3.865 ± 0.636
6.31GluGlu: 6.31 ± 0.798
2.603GluPhe: 2.603 ± 0.492
1.972GluGly: 1.972 ± 0.375
1.104GluHis: 1.104 ± 0.327
7.019GluIle: 7.019 ± 0.718
6.467GluLys: 6.467 ± 0.982
6.862GluLeu: 6.862 ± 1.04
1.814GluMet: 1.814 ± 0.496
3.707GluAsn: 3.707 ± 0.53
1.499GluPro: 1.499 ± 0.409
3.865GluGln: 3.865 ± 0.602
2.208GluArg: 2.208 ± 0.46
3.391GluSer: 3.391 ± 0.591
4.496GluThr: 4.496 ± 0.651
5.442GluVal: 5.442 ± 0.58
0.946GluTrp: 0.946 ± 0.281
2.445GluTyr: 2.445 ± 0.383
0.0GluXaa: 0.0 ± 0.0
Phe
3.155PheAla: 3.155 ± 0.405
0.158PheCys: 0.158 ± 0.108
3.47PheAsp: 3.47 ± 0.606
2.997PheGlu: 2.997 ± 0.555
1.341PhePhe: 1.341 ± 0.37
2.839PheGly: 2.839 ± 0.423
0.158PheHis: 0.158 ± 0.108
3.076PheIle: 3.076 ± 0.486
3.076PheLys: 3.076 ± 0.34
2.445PheLeu: 2.445 ± 0.465
1.341PheMet: 1.341 ± 0.309
2.051PheAsn: 2.051 ± 0.541
0.868PhePro: 0.868 ± 0.305
1.104PheGln: 1.104 ± 0.334
1.499PheArg: 1.499 ± 0.317
3.47PheSer: 3.47 ± 0.594
2.524PheThr: 2.524 ± 0.421
2.603PheVal: 2.603 ± 0.495
0.394PheTrp: 0.394 ± 0.166
2.445PheTyr: 2.445 ± 0.513
0.0PheXaa: 0.0 ± 0.0
Gly
4.338GlyAla: 4.338 ± 0.891
0.237GlyCys: 0.237 ± 0.141
3.47GlyAsp: 3.47 ± 0.46
2.76GlyGlu: 2.76 ± 0.56
2.76GlyPhe: 2.76 ± 0.436
4.89GlyGly: 4.89 ± 0.717
0.868GlyHis: 0.868 ± 0.241
5.284GlyIle: 5.284 ± 0.531
5.758GlyLys: 5.758 ± 0.846
4.811GlyLeu: 4.811 ± 0.785
2.051GlyMet: 2.051 ± 0.446
2.918GlyAsn: 2.918 ± 0.431
1.577GlyPro: 1.577 ± 0.478
2.051GlyGln: 2.051 ± 0.383
2.366GlyArg: 2.366 ± 0.485
2.918GlySer: 2.918 ± 0.593
3.944GlyThr: 3.944 ± 0.757
4.653GlyVal: 4.653 ± 0.627
1.025GlyTrp: 1.025 ± 0.233
3.628GlyTyr: 3.628 ± 0.557
0.0GlyXaa: 0.0 ± 0.0
His
0.394HisAla: 0.394 ± 0.196
0.0HisCys: 0.0 ± 0.0
1.104HisAsp: 1.104 ± 0.437
0.552HisGlu: 0.552 ± 0.238
0.868HisPhe: 0.868 ± 0.224
0.394HisGly: 0.394 ± 0.173
0.315HisHis: 0.315 ± 0.193
0.946HisIle: 0.946 ± 0.263
0.789HisLys: 0.789 ± 0.255
0.789HisLeu: 0.789 ± 0.24
0.079HisMet: 0.079 ± 0.073
0.946HisAsn: 0.946 ± 0.288
0.315HisPro: 0.315 ± 0.135
0.946HisGln: 0.946 ± 0.211
0.631HisArg: 0.631 ± 0.25
0.868HisSer: 0.868 ± 0.34
0.868HisThr: 0.868 ± 0.292
0.868HisVal: 0.868 ± 0.224
0.079HisTrp: 0.079 ± 0.08
0.473HisTyr: 0.473 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
5.758IleAla: 5.758 ± 0.585
0.315IleCys: 0.315 ± 0.17
5.521IleAsp: 5.521 ± 0.674
5.679IleGlu: 5.679 ± 0.785
2.839IlePhe: 2.839 ± 0.575
4.18IleGly: 4.18 ± 0.655
0.789IleHis: 0.789 ± 0.274
3.865IleIle: 3.865 ± 0.643
6.073IleLys: 6.073 ± 0.6
5.284IleLeu: 5.284 ± 0.799
0.946IleMet: 0.946 ± 0.283
4.732IleAsn: 4.732 ± 0.541
2.76IlePro: 2.76 ± 0.423
1.972IleGln: 1.972 ± 0.422
2.524IleArg: 2.524 ± 0.406
4.18IleSer: 4.18 ± 0.618
4.259IleThr: 4.259 ± 0.564
4.653IleVal: 4.653 ± 0.648
0.71IleTrp: 0.71 ± 0.278
3.076IleTyr: 3.076 ± 0.472
0.0IleXaa: 0.0 ± 0.0
Lys
6.625LysAla: 6.625 ± 0.831
0.473LysCys: 0.473 ± 0.208
5.836LysAsp: 5.836 ± 0.569
7.019LysGlu: 7.019 ± 1.025
2.997LysPhe: 2.997 ± 0.551
5.442LysGly: 5.442 ± 0.847
0.868LysHis: 0.868 ± 0.296
4.969LysIle: 4.969 ± 0.808
9.386LysLys: 9.386 ± 0.997
8.124LysLeu: 8.124 ± 0.745
2.76LysMet: 2.76 ± 0.54
6.31LysAsn: 6.31 ± 0.68
2.76LysPro: 2.76 ± 0.467
4.101LysGln: 4.101 ± 0.668
3.865LysArg: 3.865 ± 0.626
4.574LysSer: 4.574 ± 0.678
6.862LysThr: 6.862 ± 0.799
5.994LysVal: 5.994 ± 0.75
1.104LysTrp: 1.104 ± 0.26
3.628LysTyr: 3.628 ± 0.487
0.0LysXaa: 0.0 ± 0.0
Leu
5.284LeuAla: 5.284 ± 0.722
0.315LeuCys: 0.315 ± 0.16
4.89LeuAsp: 4.89 ± 0.533
6.231LeuGlu: 6.231 ± 0.822
2.682LeuPhe: 2.682 ± 0.506
5.127LeuGly: 5.127 ± 0.927
0.631LeuHis: 0.631 ± 0.264
5.284LeuIle: 5.284 ± 0.687
8.834LeuLys: 8.834 ± 1.126
5.915LeuLeu: 5.915 ± 0.797
1.814LeuMet: 1.814 ± 0.386
5.284LeuAsn: 5.284 ± 0.508
2.682LeuPro: 2.682 ± 0.542
3.707LeuGln: 3.707 ± 0.481
2.997LeuArg: 2.997 ± 0.467
5.521LeuSer: 5.521 ± 0.611
5.284LeuThr: 5.284 ± 0.681
4.653LeuVal: 4.653 ± 0.54
0.946LeuTrp: 0.946 ± 0.227
2.997LeuTyr: 2.997 ± 0.392
0.0LeuXaa: 0.0 ± 0.0
Met
1.577MetAla: 1.577 ± 0.268
0.158MetCys: 0.158 ± 0.122
1.735MetAsp: 1.735 ± 0.426
1.262MetGlu: 1.262 ± 0.282
0.868MetPhe: 0.868 ± 0.262
0.71MetGly: 0.71 ± 0.287
0.315MetHis: 0.315 ± 0.135
2.051MetIle: 2.051 ± 0.426
1.183MetLys: 1.183 ± 0.317
1.814MetLeu: 1.814 ± 0.385
0.789MetMet: 0.789 ± 0.237
1.262MetAsn: 1.262 ± 0.374
0.473MetPro: 0.473 ± 0.171
0.789MetGln: 0.789 ± 0.249
0.946MetArg: 0.946 ± 0.25
2.051MetSer: 2.051 ± 0.404
2.051MetThr: 2.051 ± 0.438
1.577MetVal: 1.577 ± 0.315
0.237MetTrp: 0.237 ± 0.125
0.789MetTyr: 0.789 ± 0.266
0.0MetXaa: 0.0 ± 0.0
Asn
3.707AsnAla: 3.707 ± 0.521
0.473AsnCys: 0.473 ± 0.205
3.865AsnAsp: 3.865 ± 0.503
3.786AsnGlu: 3.786 ± 0.481
2.76AsnPhe: 2.76 ± 0.417
5.363AsnGly: 5.363 ± 0.788
0.789AsnHis: 0.789 ± 0.219
4.18AsnIle: 4.18 ± 0.57
5.679AsnLys: 5.679 ± 0.683
4.417AsnLeu: 4.417 ± 0.474
0.631AsnMet: 0.631 ± 0.223
3.155AsnAsn: 3.155 ± 0.445
2.287AsnPro: 2.287 ± 0.35
1.814AsnGln: 1.814 ± 0.439
1.893AsnArg: 1.893 ± 0.411
4.574AsnSer: 4.574 ± 0.902
2.839AsnThr: 2.839 ± 0.524
3.786AsnVal: 3.786 ± 0.718
1.262AsnTrp: 1.262 ± 0.34
2.051AsnTyr: 2.051 ± 0.361
0.0AsnXaa: 0.0 ± 0.0
Pro
1.183ProAla: 1.183 ± 0.358
0.0ProCys: 0.0 ± 0.0
2.051ProAsp: 2.051 ± 0.42
1.656ProGlu: 1.656 ± 0.439
1.656ProPhe: 1.656 ± 0.328
1.183ProGly: 1.183 ± 0.394
0.315ProHis: 0.315 ± 0.174
1.814ProIle: 1.814 ± 0.332
3.313ProLys: 3.313 ± 0.53
2.445ProLeu: 2.445 ± 0.533
0.315ProMet: 0.315 ± 0.203
1.814ProAsn: 1.814 ± 0.431
0.473ProPro: 0.473 ± 0.191
1.499ProGln: 1.499 ± 0.4
1.104ProArg: 1.104 ± 0.331
2.445ProSer: 2.445 ± 0.428
1.735ProThr: 1.735 ± 0.373
1.42ProVal: 1.42 ± 0.368
0.079ProTrp: 0.079 ± 0.094
1.341ProTyr: 1.341 ± 0.419
0.0ProXaa: 0.0 ± 0.0
Gln
3.313GlnAla: 3.313 ± 0.531
0.158GlnCys: 0.158 ± 0.094
1.656GlnAsp: 1.656 ± 0.308
2.918GlnGlu: 2.918 ± 0.586
1.499GlnPhe: 1.499 ± 0.355
2.366GlnGly: 2.366 ± 0.557
0.394GlnHis: 0.394 ± 0.148
2.76GlnIle: 2.76 ± 0.47
3.786GlnLys: 3.786 ± 0.479
3.549GlnLeu: 3.549 ± 0.491
1.104GlnMet: 1.104 ± 0.329
1.735GlnAsn: 1.735 ± 0.408
0.394GlnPro: 0.394 ± 0.202
1.735GlnGln: 1.735 ± 0.459
1.656GlnArg: 1.656 ± 0.465
3.944GlnSer: 3.944 ± 0.435
2.603GlnThr: 2.603 ± 0.447
2.13GlnVal: 2.13 ± 0.409
0.631GlnTrp: 0.631 ± 0.145
0.946GlnTyr: 0.946 ± 0.29
0.0GlnXaa: 0.0 ± 0.0
Arg
1.893ArgAla: 1.893 ± 0.402
0.079ArgCys: 0.079 ± 0.081
1.972ArgAsp: 1.972 ± 0.39
2.682ArgGlu: 2.682 ± 0.542
1.577ArgPhe: 1.577 ± 0.24
2.13ArgGly: 2.13 ± 0.304
0.868ArgHis: 0.868 ± 0.27
3.549ArgIle: 3.549 ± 0.516
3.944ArgLys: 3.944 ± 0.738
2.997ArgLeu: 2.997 ± 0.571
0.946ArgMet: 0.946 ± 0.258
2.13ArgAsn: 2.13 ± 0.417
1.42ArgPro: 1.42 ± 0.303
1.735ArgGln: 1.735 ± 0.351
1.341ArgArg: 1.341 ± 0.369
1.735ArgSer: 1.735 ± 0.39
1.972ArgThr: 1.972 ± 0.37
1.814ArgVal: 1.814 ± 0.396
0.71ArgTrp: 0.71 ± 0.243
1.972ArgTyr: 1.972 ± 0.421
0.0ArgXaa: 0.0 ± 0.0
Ser
3.865SerAla: 3.865 ± 0.826
0.158SerCys: 0.158 ± 0.114
5.915SerAsp: 5.915 ± 0.863
3.786SerGlu: 3.786 ± 0.382
3.628SerPhe: 3.628 ± 0.588
4.811SerGly: 4.811 ± 0.678
1.104SerHis: 1.104 ± 0.35
4.022SerIle: 4.022 ± 0.551
5.048SerLys: 5.048 ± 0.753
4.338SerLeu: 4.338 ± 0.533
1.577SerMet: 1.577 ± 0.368
4.574SerAsn: 4.574 ± 0.719
0.71SerPro: 0.71 ± 0.33
3.234SerGln: 3.234 ± 0.513
2.682SerArg: 2.682 ± 0.427
5.127SerSer: 5.127 ± 0.588
3.865SerThr: 3.865 ± 0.885
4.101SerVal: 4.101 ± 0.685
0.315SerTrp: 0.315 ± 0.137
2.603SerTyr: 2.603 ± 0.439
0.0SerXaa: 0.0 ± 0.0
Thr
4.101ThrAla: 4.101 ± 0.844
0.315ThrCys: 0.315 ± 0.187
2.682ThrAsp: 2.682 ± 0.42
3.549ThrGlu: 3.549 ± 0.532
3.155ThrPhe: 3.155 ± 0.536
4.653ThrGly: 4.653 ± 0.572
0.868ThrHis: 0.868 ± 0.256
4.89ThrIle: 4.89 ± 0.849
6.704ThrLys: 6.704 ± 0.836
5.915ThrLeu: 5.915 ± 0.786
0.946ThrMet: 0.946 ± 0.352
3.47ThrAsn: 3.47 ± 0.415
2.603ThrPro: 2.603 ± 0.457
2.287ThrGln: 2.287 ± 0.385
2.208ThrArg: 2.208 ± 0.413
3.47ThrSer: 3.47 ± 0.711
4.574ThrThr: 4.574 ± 0.547
5.048ThrVal: 5.048 ± 1.072
0.552ThrTrp: 0.552 ± 0.181
2.682ThrTyr: 2.682 ± 0.595
0.0ThrXaa: 0.0 ± 0.0
Val
4.338ValAla: 4.338 ± 0.564
0.394ValCys: 0.394 ± 0.143
5.048ValAsp: 5.048 ± 0.665
5.363ValGlu: 5.363 ± 0.639
1.42ValPhe: 1.42 ± 0.345
4.259ValGly: 4.259 ± 0.692
0.473ValHis: 0.473 ± 0.186
3.155ValIle: 3.155 ± 0.504
6.389ValLys: 6.389 ± 0.714
5.6ValLeu: 5.6 ± 0.545
1.183ValMet: 1.183 ± 0.32
3.786ValAsn: 3.786 ± 0.534
1.656ValPro: 1.656 ± 0.398
1.656ValGln: 1.656 ± 0.363
2.603ValArg: 2.603 ± 0.484
4.969ValSer: 4.969 ± 0.898
5.127ValThr: 5.127 ± 0.537
4.259ValVal: 4.259 ± 0.635
0.473ValTrp: 0.473 ± 0.193
1.262ValTyr: 1.262 ± 0.232
0.0ValXaa: 0.0 ± 0.0
Trp
0.868TrpAla: 0.868 ± 0.255
0.158TrpCys: 0.158 ± 0.112
0.473TrpAsp: 0.473 ± 0.193
0.631TrpGlu: 0.631 ± 0.255
0.237TrpPhe: 0.237 ± 0.116
0.946TrpGly: 0.946 ± 0.325
0.315TrpHis: 0.315 ± 0.128
1.104TrpIle: 1.104 ± 0.252
1.025TrpLys: 1.025 ± 0.261
1.104TrpLeu: 1.104 ± 0.338
0.237TrpMet: 0.237 ± 0.128
0.631TrpAsn: 0.631 ± 0.304
0.158TrpPro: 0.158 ± 0.097
0.71TrpGln: 0.71 ± 0.282
0.71TrpArg: 0.71 ± 0.242
0.789TrpSer: 0.789 ± 0.233
0.631TrpThr: 0.631 ± 0.303
0.394TrpVal: 0.394 ± 0.124
0.158TrpTrp: 0.158 ± 0.11
0.631TrpTyr: 0.631 ± 0.191
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.549TyrAla: 3.549 ± 0.671
0.71TyrCys: 0.71 ± 0.205
2.918TyrAsp: 2.918 ± 0.437
2.76TyrGlu: 2.76 ± 0.659
1.814TyrPhe: 1.814 ± 0.365
2.287TyrGly: 2.287 ± 0.421
0.789TyrHis: 0.789 ± 0.277
1.893TyrIle: 1.893 ± 0.512
3.313TyrLys: 3.313 ± 0.578
3.944TyrLeu: 3.944 ± 0.437
1.025TyrMet: 1.025 ± 0.233
2.366TyrAsn: 2.366 ± 0.467
1.499TyrPro: 1.499 ± 0.438
1.893TyrGln: 1.893 ± 0.396
1.262TyrArg: 1.262 ± 0.339
2.445TyrSer: 2.445 ± 0.431
2.524TyrThr: 2.524 ± 0.557
2.208TyrVal: 2.208 ± 0.5
0.552TyrTrp: 0.552 ± 0.207
1.814TyrTyr: 1.814 ± 0.509
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (12680 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski