Costertonia aggregata

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Costertonia

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3525 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7H9ALA8|A0A7H9ALA8_9FLAO Preprotein translocase subunit SecE OS=Costertonia aggregata OX=343403 GN=secE PE=4 SV=1
MM1 pKa = 7.56TIISKK6 pKa = 10.7NKK8 pKa = 9.18TILLFLFILSSIGYY22 pKa = 8.24GQTTIWEE29 pKa = 4.28EE30 pKa = 3.97DD31 pKa = 3.48FEE33 pKa = 5.6SYY35 pKa = 11.76GNGTSTGAGSGASTATWSTNDD56 pKa = 2.65GDD58 pKa = 3.74IDD60 pKa = 3.67VRR62 pKa = 11.84TISGNKK68 pKa = 7.69VLRR71 pKa = 11.84GSDD74 pKa = 3.29TRR76 pKa = 11.84NTSARR81 pKa = 11.84WTTNPIDD88 pKa = 3.2ISGYY92 pKa = 7.4TNVSFSLDD100 pKa = 3.45ADD102 pKa = 3.76SGGGLDD108 pKa = 3.64SGVDD112 pKa = 3.31VFRR115 pKa = 11.84IQYY118 pKa = 10.05RR119 pKa = 11.84IDD121 pKa = 3.41GGSYY125 pKa = 9.93IEE127 pKa = 4.76IEE129 pKa = 4.28NTSGDD134 pKa = 3.83TSPSEE139 pKa = 4.58PIQPSYY145 pKa = 10.95GVSGLSGSTLEE156 pKa = 5.44FRR158 pKa = 11.84ITMYY162 pKa = 9.59NTTGAEE168 pKa = 4.5FYY170 pKa = 10.69DD171 pKa = 3.46IDD173 pKa = 4.83NILVQGTLPTPDD185 pKa = 4.43CSTISSFPGSFIASNTTLNSNLSGFSSGIITDD217 pKa = 3.62VNVSLNITHH226 pKa = 6.92TWNADD231 pKa = 3.1LDD233 pKa = 3.77ISLISPSGTTVVLSSDD249 pKa = 3.43NGGSGDD255 pKa = 3.63NYY257 pKa = 10.67VNTFFDD263 pKa = 4.22DD264 pKa = 3.61SGSINIISGSSPFTGTFRR282 pKa = 11.84PEE284 pKa = 3.78GNLSDD289 pKa = 4.89FNGEE293 pKa = 4.08NANGTWVLRR302 pKa = 11.84VFDD305 pKa = 4.41DD306 pKa = 3.59TGGDD310 pKa = 3.28TGTLDD315 pKa = 3.44GFSIEE320 pKa = 3.9ICTNSSTDD328 pKa = 3.59LAVSKK333 pKa = 9.54TVDD336 pKa = 3.74DD337 pKa = 4.06NTPSEE342 pKa = 4.22GSNIVYY348 pKa = 8.79TLSVEE353 pKa = 4.33NNGPLDD359 pKa = 3.78ATNVTLTDD367 pKa = 3.52VLPAGVSFVSDD378 pKa = 3.33DD379 pKa = 3.38GAYY382 pKa = 10.49NSSTGVWSVGSLNNGATAILNITASVDD409 pKa = 3.09GSTSGSTITNTITSISADD427 pKa = 3.43QTDD430 pKa = 4.15SNLTADD436 pKa = 4.94DD437 pKa = 4.24LSEE440 pKa = 4.91SIVPSVDD447 pKa = 3.11QPPVVTVTGNQIYY460 pKa = 10.39CPLTSLSIAEE470 pKa = 4.77TISISDD476 pKa = 4.21PDD478 pKa = 4.23DD479 pKa = 3.52TTTDD483 pKa = 2.9AVYY486 pKa = 10.12IQISSGYY493 pKa = 10.05VNGEE497 pKa = 4.16DD498 pKa = 5.16LLTLTGTHH506 pKa = 6.76PNITATWDD514 pKa = 4.03AIEE517 pKa = 5.68GEE519 pKa = 4.47LTLQGPTTYY528 pKa = 10.79SEE530 pKa = 4.57FEE532 pKa = 4.15TAVLAVEE539 pKa = 4.46YY540 pKa = 10.05SSSASAPTGIKK551 pKa = 9.89QFSITPGSANFLPPTGHH568 pKa = 6.19YY569 pKa = 10.8YY570 pKa = 10.79EE571 pKa = 5.65FISDD575 pKa = 5.56LGITWTDD582 pKa = 3.07ARR584 pKa = 11.84DD585 pKa = 3.28AAALRR590 pKa = 11.84TYY592 pKa = 10.69FGLQGYY598 pKa = 8.27LATLTTQAEE607 pKa = 4.27ADD609 pKa = 3.88FSGTQAVGVGWIGASDD625 pKa = 3.68AANEE629 pKa = 4.22GDD631 pKa = 3.73WRR633 pKa = 11.84WVTGPEE639 pKa = 3.71AGTPFWSGTSTGTTVAPTNFAYY661 pKa = 9.81WNGGEE666 pKa = 4.26PNQSGNEE673 pKa = 4.05DD674 pKa = 3.65YY675 pKa = 11.83AHH677 pKa = 6.34ITDD680 pKa = 4.1PSVVRR685 pKa = 11.84GSGGLGSWNDD695 pKa = 3.57LSNTGASSGPYY706 pKa = 8.87QPQGYY711 pKa = 8.11VVEE714 pKa = 4.51YY715 pKa = 9.83GGSPGDD721 pKa = 3.4PVLNITGVTTITVDD735 pKa = 3.75NINPTASNPSPVTVFCPSDD754 pKa = 3.59VPTFDD759 pKa = 3.06ISVVTDD765 pKa = 3.34EE766 pKa = 5.81ADD768 pKa = 3.34NCTTNPLVTFVSDD781 pKa = 3.39VSNGGSNPEE790 pKa = 4.3IITRR794 pKa = 11.84TYY796 pKa = 10.41RR797 pKa = 11.84ISDD800 pKa = 3.51DD801 pKa = 3.37MGNFIDD807 pKa = 3.91VAQTITVDD815 pKa = 4.41PINIDD820 pKa = 3.79TQPLDD825 pKa = 2.91QTTFVNNNASFSVSINNVDD844 pKa = 3.53TYY846 pKa = 10.59QWQVSIDD853 pKa = 3.39GGTNFIDD860 pKa = 3.38ISDD863 pKa = 3.76GTEE866 pKa = 3.72YY867 pKa = 10.54TGTSTSTLTVIAPRR881 pKa = 11.84VDD883 pKa = 3.12KK884 pKa = 11.27NNYY887 pKa = 7.81RR888 pKa = 11.84YY889 pKa = 9.55RR890 pKa = 11.84VAASNSGSGCSMVISNNALLSVRR913 pKa = 11.84VSSVITNRR921 pKa = 11.84RR922 pKa = 11.84ITYY925 pKa = 9.44RR926 pKa = 11.84VKK928 pKa = 11.07NNN930 pKa = 3.07

Molecular weight: 97.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7H9AKM5|A0A7H9AKM5_9FLAO VOC family protein OS=Costertonia aggregata OX=343403 GN=HYG79_01080 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 10.15RR11 pKa = 11.84KK12 pKa = 9.6RR13 pKa = 11.84KK14 pKa = 8.29NKK16 pKa = 9.34HH17 pKa = 4.03GFRR20 pKa = 11.84EE21 pKa = 4.05RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.08GRR40 pKa = 11.84KK41 pKa = 8.3KK42 pKa = 9.83LTVSSEE48 pKa = 3.9PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3525

0

3525

1168523

34

4800

331.5

37.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.411 ± 0.041

0.738 ± 0.014

5.86 ± 0.045

6.575 ± 0.038

5.237 ± 0.034

6.685 ± 0.055

1.783 ± 0.023

7.62 ± 0.041

7.757 ± 0.068

9.261 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.284 ± 0.024

5.88 ± 0.039

3.474 ± 0.027

3.296 ± 0.022

3.66 ± 0.028

6.299 ± 0.029

5.824 ± 0.06

6.287 ± 0.032

1.091 ± 0.017

3.978 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski