Lactobacillus virus c5
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F8J191|F8J191_9CAUD Uncharacterized protein OS=Lactobacillus virus c5 OX=508419 PE=4 SV=1
MM1 pKa = 7.16 TLVNEE6 pKa = 4.22 LLAYY10 pKa = 10.22 YY11 pKa = 10.31 KK12 pKa = 10.95 EE13 pKa = 4.07 NTEE16 pKa = 4.72 DD17 pKa = 3.63 FNSDD21 pKa = 3.39 IEE23 pKa = 4.9 EE24 pKa = 4.31 LDD26 pKa = 3.12 SWAGYY31 pKa = 10.29 LGDD34 pKa = 3.69 EE35 pKa = 3.99 RR36 pKa = 11.84 VVPMEE41 pKa = 4.37 EE42 pKa = 5.24 LSDD45 pKa = 4.2 FYY47 pKa = 11.39 QGVDD51 pKa = 3.23 PLEE54 pKa = 3.92 VLRR57 pKa = 11.84 RR58 pKa = 11.84 AFFGYY63 pKa = 10.72 DD64 pKa = 3.4 DD65 pKa = 4.28 VNSTSEE71 pKa = 4.15 KK72 pKa = 10.35 RR73 pKa = 11.84 EE74 pKa = 3.71 QFNPNRR80 pKa = 11.84 DD81 pKa = 3.35 YY82 pKa = 11.12 FYY84 pKa = 11.18 LNGYY88 pKa = 9.14 GISS91 pKa = 3.5
Molecular weight: 10.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.961
IPC_protein 3.884
Toseland 3.694
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.617
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.139
Thurlkill 3.745
EMBOSS 3.77
Sillero 3.999
Patrickios 1.888
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.902
Protein with the highest isoelectric point:
>tr|F8J179|F8J179_9CAUD Putative Cro-like repressor OS=Lactobacillus virus c5 OX=508419 PE=4 SV=1
MM1 pKa = 7.94 RR2 pKa = 11.84 YY3 pKa = 9.82 ISITEE8 pKa = 4.06 SLEE11 pKa = 3.68 ATHH14 pKa = 6.84 ISKK17 pKa = 10.76 KK18 pKa = 8.02 SLRR21 pKa = 11.84 EE22 pKa = 3.81 CKK24 pKa = 9.83 MKK26 pKa = 10.9 EE27 pKa = 3.81 NFKK30 pKa = 10.88 KK31 pKa = 10.65 VVAFLIRR38 pKa = 11.84 SSTMNFGSNTDD49 pKa = 3.0 IVFSFFKK56 pKa = 10.45 SFKK59 pKa = 8.59 SSSVIPYY66 pKa = 7.27 VTSSMRR72 pKa = 11.84 FVDD75 pKa = 3.67
Molecular weight: 8.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.022
IPC2_protein 9.18
IPC_protein 9.151
Toseland 10.101
ProMoST 9.633
Dawson 10.233
Bjellqvist 9.838
Wikipedia 10.35
Rodwell 10.877
Grimsley 10.277
Solomon 10.277
Lehninger 10.262
Nozaki 10.087
DTASelect 9.823
Thurlkill 10.101
EMBOSS 10.482
Sillero 10.145
Patrickios 10.672
IPC_peptide 10.277
IPC2_peptide 8.375
IPC2.peptide.svr19 8.141
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
50
0
50
9987
32
968
199.7
22.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.609 ± 0.589
0.961 ± 0.146
6.589 ± 0.442
6.929 ± 0.472
3.805 ± 0.199
6.599 ± 0.344
1.372 ± 0.169
6.919 ± 0.283
8.431 ± 0.413
7.7 ± 0.299
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.563 ± 0.192
6.689 ± 0.347
2.443 ± 0.174
3.324 ± 0.294
4.115 ± 0.254
6.418 ± 0.269
5.888 ± 0.338
7.179 ± 0.198
1.011 ± 0.103
4.456 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here