Photobacterium gaetbulicola Gung47

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Photobacterium; Photobacterium gaetbulicola

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4974 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5WY30|A0A0C5WY30_9GAMM Imidazole glycerol phosphate synthase subunit HisH OS=Photobacterium gaetbulicola Gung47 OX=658445 GN=hisH PE=3 SV=1
MM1 pKa = 7.65KK2 pKa = 9.0KK3 pKa = 8.22TLLALSVLAAGSAQAGVNLYY23 pKa = 10.43DD24 pKa = 3.77EE25 pKa = 4.98NGVIVDD31 pKa = 4.26LSAAAEE37 pKa = 3.88VQYY40 pKa = 10.64IQEE43 pKa = 4.24YY44 pKa = 8.64TPNSDD49 pKa = 3.05PFLRR53 pKa = 11.84LDD55 pKa = 5.31DD56 pKa = 5.17GDD58 pKa = 4.33LALNTTIAVNSQLNAVAGIAFAFEE82 pKa = 4.67DD83 pKa = 3.86SDD85 pKa = 4.5VKK87 pKa = 11.12NDD89 pKa = 3.79EE90 pKa = 4.17LWVGLGGDD98 pKa = 4.19FGTVTFGRR106 pKa = 11.84QLLIADD112 pKa = 4.34DD113 pKa = 3.98SGIGKK118 pKa = 10.02DD119 pKa = 3.81YY120 pKa = 11.24EE121 pKa = 4.36LGGDD125 pKa = 3.65GLDD128 pKa = 3.62FVQAEE133 pKa = 4.17GDD135 pKa = 3.57QTVKK139 pKa = 10.72YY140 pKa = 10.6VYY142 pKa = 10.83DD143 pKa = 3.54NGQFYY148 pKa = 10.96AGISAVIAEE157 pKa = 4.59DD158 pKa = 4.21NKK160 pKa = 10.73DD161 pKa = 3.39TTADD165 pKa = 3.38TSIVDD170 pKa = 3.48GRR172 pKa = 11.84LGARR176 pKa = 11.84FGNFDD181 pKa = 3.4ARR183 pKa = 11.84VYY185 pKa = 10.24LYY187 pKa = 10.71SGSDD191 pKa = 3.33VSGDD195 pKa = 3.39FVSFFGQNGSLDD207 pKa = 3.22IDD209 pKa = 4.31GYY211 pKa = 10.8NLEE214 pKa = 4.97AEE216 pKa = 4.46YY217 pKa = 11.08FMDD220 pKa = 4.82AFALAASFGQIEE232 pKa = 4.45YY233 pKa = 10.92EE234 pKa = 4.52DD235 pKa = 3.51ATSANKK241 pKa = 10.49GKK243 pKa = 10.73VEE245 pKa = 4.46ADD247 pKa = 3.35TAALAGSYY255 pKa = 9.62TMNQTTFALGYY266 pKa = 9.01TYY268 pKa = 10.64LGWDD272 pKa = 3.99EE273 pKa = 4.89KK274 pKa = 9.97PTAGGKK280 pKa = 8.4TSDD283 pKa = 4.7DD284 pKa = 3.6VNTFYY289 pKa = 11.73ANVTQQLHH297 pKa = 6.39SNVKK301 pKa = 9.03AYY303 pKa = 10.71GEE305 pKa = 3.94IGYY308 pKa = 8.51TDD310 pKa = 4.27ADD312 pKa = 3.87DD313 pKa = 5.57AEE315 pKa = 4.4FGYY318 pKa = 10.6VVGMEE323 pKa = 4.3VVFF326 pKa = 4.62

Molecular weight: 34.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5WPV6|A0A0C5WPV6_9GAMM Uncharacterized protein OS=Photobacterium gaetbulicola Gung47 OX=658445 GN=H744_2c2497 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44NGRR28 pKa = 11.84ATINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSKK44 pKa = 10.84

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4974

0

4974

1665140

28

5857

334.8

37.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.107 ± 0.043

1.101 ± 0.013

5.479 ± 0.031

6.159 ± 0.035

4.076 ± 0.023

7.158 ± 0.03

2.308 ± 0.018

6.101 ± 0.03

4.786 ± 0.027

10.349 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.805 ± 0.018

4.009 ± 0.022

4.155 ± 0.021

4.622 ± 0.033

4.73 ± 0.028

6.356 ± 0.032

5.246 ± 0.024

7.032 ± 0.029

1.303 ± 0.013

3.118 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski