Noviherbaspirillum sp. Root189

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Noviherbaspirillum; unclassified Noviherbaspirillum

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5322 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8PJY0|A0A0Q8PJY0_9BURK tRNA pseudouridine synthase B OS=Noviherbaspirillum sp. Root189 OX=1736487 GN=truB PE=3 SV=1
MM1 pKa = 7.66PFVGPLTPGPVGDD14 pKa = 4.42PLEE17 pKa = 4.58PLPMVPTPCVVGLVPVLSPDD37 pKa = 3.32APVPRR42 pKa = 11.84APDD45 pKa = 3.53VPPDD49 pKa = 3.42VADD52 pKa = 4.03AQLVLPADD60 pKa = 4.21EE61 pKa = 4.95VVPAPPVPPVLLLRR75 pKa = 11.84PLALASLLVTPLVVPGAQSLLPAADD100 pKa = 3.66VPGPPAPVVEE110 pKa = 4.31PLEE113 pKa = 4.51PEE115 pKa = 4.19PAWVLGSTEE124 pKa = 4.24LLLPIVLEE132 pKa = 4.04LTPGEE137 pKa = 4.45LGVEE141 pKa = 4.56VCAHH145 pKa = 6.79ANCEE149 pKa = 4.06AASNAASNIDD159 pKa = 3.24VEE161 pKa = 5.23VFMFPPWDD169 pKa = 3.21ISVNPHH175 pKa = 4.43VQQGACHH182 pKa = 6.71FGG184 pKa = 3.2

Molecular weight: 18.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8REY4|A0A0Q8REY4_9BURK Uncharacterized protein OS=Noviherbaspirillum sp. Root189 OX=1736487 GN=ASE07_14485 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.57RR14 pKa = 11.84THH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5322

0

5322

1677877

44

4256

315.3

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.294 ± 0.044

0.939 ± 0.011

5.329 ± 0.025

5.643 ± 0.035

3.698 ± 0.023

7.792 ± 0.035

2.281 ± 0.018

5.338 ± 0.02

4.033 ± 0.036

10.225 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.707 ± 0.018

3.173 ± 0.022

4.833 ± 0.024

3.874 ± 0.022

6.379 ± 0.037

5.829 ± 0.028

5.411 ± 0.034

7.445 ± 0.026

1.273 ± 0.013

2.5 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski