Amino acid dipepetide frequency for Noviherbaspirillum sp. Root189

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.77AlaAla: 13.77 ± 0.118	1.16AlaCys: 1.16 ± 0.028	5.869AlaAsp: 5.869 ± 0.067	6.377AlaGlu: 6.377 ± 0.086	3.908AlaPhe: 3.908 ± 0.054	9.071AlaGly: 9.071 ± 0.088	2.374AlaHis: 2.374 ± 0.039	6.037AlaIle: 6.037 ± 0.062	4.387AlaLys: 4.387 ± 0.067	12.199AlaLeu: 12.199 ± 0.119	3.349AlaMet: 3.349 ± 0.046	3.307AlaAsn: 3.307 ± 0.046	4.807AlaPro: 4.807 ± 0.072	4.6AlaGln: 4.6 ± 0.062	7.141AlaArg: 7.141 ± 0.093	6.638AlaSer: 6.638 ± 0.073	5.739AlaThr: 5.739 ± 0.075	8.11AlaVal: 8.11 ± 0.08	1.377AlaTrp: 1.377 ± 0.03	2.712AlaTyr: 2.712 ± 0.046	0.005AlaXaa: 0.005 ± 0.002
Cys	1.036CysAla: 1.036 ± 0.026	0.131CysCys: 0.131 ± 0.01	0.56CysAsp: 0.56 ± 0.018	0.511CysGlu: 0.511 ± 0.018	0.353CysPhe: 0.353 ± 0.017	0.956CysGly: 0.956 ± 0.025	0.272CysHis: 0.272 ± 0.013	0.485CysIle: 0.485 ± 0.018	0.297CysLys: 0.297 ± 0.014	0.819CysLeu: 0.819 ± 0.024	0.253CysMet: 0.253 ± 0.013	0.29CysAsn: 0.29 ± 0.014	0.464CysPro: 0.464 ± 0.015	0.262CysGln: 0.262 ± 0.011	0.614CysArg: 0.614 ± 0.021	0.558CysSer: 0.558 ± 0.019	0.513CysThr: 0.513 ± 0.014	0.676CysVal: 0.676 ± 0.019	0.111CysTrp: 0.111 ± 0.008	0.234CysTyr: 0.234 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.303AspAla: 6.303 ± 0.074	0.493AspCys: 0.493 ± 0.019	2.798AspAsp: 2.798 ± 0.046	3.31AspGlu: 3.31 ± 0.049	2.117AspPhe: 2.117 ± 0.037	4.307AspGly: 4.307 ± 0.061	1.107AspHis: 1.107 ± 0.026	3.12AspIle: 3.12 ± 0.047	2.212AspLys: 2.212 ± 0.04	5.266AspLeu: 5.266 ± 0.061	1.473AspMet: 1.473 ± 0.031	1.541AspAsn: 1.541 ± 0.032	2.719AspPro: 2.719 ± 0.041	1.638AspGln: 1.638 ± 0.036	3.224AspArg: 3.224 ± 0.047	2.48AspSer: 2.48 ± 0.038	2.773AspThr: 2.773 ± 0.044	4.083AspVal: 4.083 ± 0.053	0.829AspTrp: 0.829 ± 0.022	1.494AspTyr: 1.494 ± 0.031	0.001AspXaa: 0.001 ± 0.001
Glu	6.313GluAla: 6.313 ± 0.082	0.441GluCys: 0.441 ± 0.015	2.464GluAsp: 2.464 ± 0.038	3.378GluGlu: 3.378 ± 0.058	2.012GluPhe: 2.012 ± 0.041	3.54GluGly: 3.54 ± 0.052	1.407GluHis: 1.407 ± 0.032	3.316GluIle: 3.316 ± 0.047	2.746GluLys: 2.746 ± 0.047	5.924GluLeu: 5.924 ± 0.066	1.541GluMet: 1.541 ± 0.032	1.744GluAsn: 1.744 ± 0.037	2.258GluPro: 2.258 ± 0.045	2.922GluGln: 2.922 ± 0.047	4.409GluArg: 4.409 ± 0.068	2.985GluSer: 2.985 ± 0.046	2.865GluThr: 2.865 ± 0.043	4.066GluVal: 4.066 ± 0.052	0.78GluTrp: 0.78 ± 0.022	1.317GluTyr: 1.317 ± 0.028	0.005GluXaa: 0.005 ± 0.002
Phe	4.073PheAla: 4.073 ± 0.053	0.448PheCys: 0.448 ± 0.015	2.358PheAsp: 2.358 ± 0.039	1.993PheGlu: 1.993 ± 0.034	1.518PhePhe: 1.518 ± 0.035	3.392PheGly: 3.392 ± 0.051	0.808PheHis: 0.808 ± 0.021	1.932PheIle: 1.932 ± 0.034	1.302PheLys: 1.302 ± 0.027	3.301PheLeu: 3.301 ± 0.053	0.87PheMet: 0.87 ± 0.026	1.361PheAsn: 1.361 ± 0.031	1.615PhePro: 1.615 ± 0.029	1.102PheGln: 1.102 ± 0.026	2.087PheArg: 2.087 ± 0.036	2.574PheSer: 2.574 ± 0.039	2.044PheThr: 2.044 ± 0.044	2.715PheVal: 2.715 ± 0.049	0.483PheTrp: 0.483 ± 0.018	1.01PheTyr: 1.01 ± 0.027	0.001PheXaa: 0.001 ± 0.001
Gly	7.604GlyAla: 7.604 ± 0.078	0.812GlyCys: 0.812 ± 0.021	3.732GlyAsp: 3.732 ± 0.05	4.097GlyGlu: 4.097 ± 0.059	3.257GlyPhe: 3.257 ± 0.049	6.099GlyGly: 6.099 ± 0.08	1.778GlyHis: 1.778 ± 0.038	4.739GlyIle: 4.739 ± 0.054	4.136GlyLys: 4.136 ± 0.052	7.805GlyLeu: 7.805 ± 0.077	2.562GlyMet: 2.562 ± 0.046	2.55GlyAsn: 2.55 ± 0.051	2.653GlyPro: 2.653 ± 0.039	2.899GlyGln: 2.899 ± 0.045	4.665GlyArg: 4.665 ± 0.061	4.569GlySer: 4.569 ± 0.063	4.474GlyThr: 4.474 ± 0.068	5.97GlyVal: 5.97 ± 0.072	1.144GlyTrp: 1.144 ± 0.029	2.371GlyTyr: 2.371 ± 0.043	0.003GlyXaa: 0.003 ± 0.001
His	2.745HisAla: 2.745 ± 0.045	0.277HisCys: 0.277 ± 0.012	1.277HisAsp: 1.277 ± 0.03	1.236HisGlu: 1.236 ± 0.027	0.937HisPhe: 0.937 ± 0.026	1.98HisGly: 1.98 ± 0.04	0.682HisHis: 0.682 ± 0.021	1.183HisIle: 1.183 ± 0.025	0.672HisLys: 0.672 ± 0.018	2.134HisLeu: 2.134 ± 0.035	0.579HisMet: 0.579 ± 0.018	0.568HisAsn: 0.568 ± 0.018	1.397HisPro: 1.397 ± 0.035	0.83HisGln: 0.83 ± 0.023	1.398HisArg: 1.398 ± 0.03	1.153HisSer: 1.153 ± 0.027	1.078HisThr: 1.078 ± 0.024	1.65HisVal: 1.65 ± 0.033	0.353HisTrp: 0.353 ± 0.013	0.679HisTyr: 0.679 ± 0.021	0.002HisXaa: 0.002 ± 0.001
Ile	7.048IleAla: 7.048 ± 0.07	0.53IleCys: 0.53 ± 0.017	3.6IleAsp: 3.6 ± 0.048	3.527IleGlu: 3.527 ± 0.045	1.623IlePhe: 1.623 ± 0.039	4.882IleGly: 4.882 ± 0.058	1.039IleHis: 1.039 ± 0.03	2.33IleIle: 2.33 ± 0.041	1.989IleLys: 1.989 ± 0.045	4.406IleLeu: 4.406 ± 0.055	1.073IleMet: 1.073 ± 0.024	1.913IleAsn: 1.913 ± 0.031	2.494IlePro: 2.494 ± 0.038	1.591IleGln: 1.591 ± 0.029	3.159IleArg: 3.159 ± 0.041	3.166IleSer: 3.166 ± 0.039	2.975IleThr: 2.975 ± 0.047	4.268IleVal: 4.268 ± 0.045	0.549IleTrp: 0.549 ± 0.019	1.216IleTyr: 1.216 ± 0.026	0.001IleXaa: 0.001 ± 0.001
Lys	4.426LysAla: 4.426 ± 0.068	0.222LysCys: 0.222 ± 0.011	2.047LysAsp: 2.047 ± 0.04	2.338LysGlu: 2.338 ± 0.038	1.192LysPhe: 1.192 ± 0.028	2.684LysGly: 2.684 ± 0.042	0.84LysHis: 0.84 ± 0.023	2.172LysIle: 2.172 ± 0.038	1.944LysLys: 1.944 ± 0.055	4.234LysLeu: 4.234 ± 0.056	1.153LysMet: 1.153 ± 0.028	1.403LysAsn: 1.403 ± 0.033	2.365LysPro: 2.365 ± 0.049	1.667LysGln: 1.667 ± 0.033	2.655LysArg: 2.655 ± 0.044	2.304LysSer: 2.304 ± 0.047	2.422LysThr: 2.422 ± 0.047	2.913LysVal: 2.913 ± 0.048	0.474LysTrp: 0.474 ± 0.018	0.87LysTyr: 0.87 ± 0.028	0.002LysXaa: 0.002 ± 0.001
Leu	12.254LeuAla: 12.254 ± 0.099	1.017LeuCys: 1.017 ± 0.027	5.435LeuAsp: 5.435 ± 0.065	5.354LeuGlu: 5.354 ± 0.063	3.695LeuPhe: 3.695 ± 0.056	7.643LeuGly: 7.643 ± 0.077	2.326LeuHis: 2.326 ± 0.044	5.075LeuIle: 5.075 ± 0.065	4.166LeuLys: 4.166 ± 0.046	10.553LeuLeu: 10.553 ± 0.111	2.534LeuMet: 2.534 ± 0.045	3.273LeuAsn: 3.273 ± 0.047	5.531LeuPro: 5.531 ± 0.065	3.978LeuGln: 3.978 ± 0.059	6.743LeuArg: 6.743 ± 0.075	6.728LeuSer: 6.728 ± 0.074	5.512LeuThr: 5.512 ± 0.063	7.093LeuVal: 7.093 ± 0.08	1.087LeuTrp: 1.087 ± 0.029	2.251LeuTyr: 2.251 ± 0.042	0.003LeuXaa: 0.003 ± 0.002
Met	2.901MetAla: 2.901 ± 0.039	0.194MetCys: 0.194 ± 0.011	1.249MetAsp: 1.249 ± 0.029	1.35MetGlu: 1.35 ± 0.038	0.827MetPhe: 0.827 ± 0.021	1.802MetGly: 1.802 ± 0.035	0.657MetHis: 0.657 ± 0.023	1.273MetIle: 1.273 ± 0.03	1.269MetLys: 1.269 ± 0.031	3.004MetLeu: 3.004 ± 0.043	0.744MetMet: 0.744 ± 0.023	1.011MetAsn: 1.011 ± 0.026	1.579MetPro: 1.579 ± 0.036	1.274MetGln: 1.274 ± 0.029	1.811MetArg: 1.811 ± 0.032	1.754MetSer: 1.754 ± 0.033	1.766MetThr: 1.766 ± 0.032	1.874MetVal: 1.874 ± 0.035	0.219MetTrp: 0.219 ± 0.011	0.514MetTyr: 0.514 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.819AsnAla: 3.819 ± 0.047	0.302AsnCys: 0.302 ± 0.014	1.726AsnAsp: 1.726 ± 0.034	1.711AsnGlu: 1.711 ± 0.036	1.129AsnPhe: 1.129 ± 0.023	2.745AsnGly: 2.745 ± 0.049	0.628AsnHis: 0.628 ± 0.02	1.598AsnIle: 1.598 ± 0.036	1.204AsnLys: 1.204 ± 0.023	3.029AsnLeu: 3.029 ± 0.044	0.78AsnMet: 0.78 ± 0.025	1.035AsnAsn: 1.035 ± 0.025	1.984AsnPro: 1.984 ± 0.036	1.083AsnGln: 1.083 ± 0.026	1.973AsnArg: 1.973 ± 0.038	1.573AsnSer: 1.573 ± 0.033	1.746AsnThr: 1.746 ± 0.034	2.415AsnVal: 2.415 ± 0.044	0.426AsnTrp: 0.426 ± 0.015	0.827AsnTyr: 0.827 ± 0.023	0.001AsnXaa: 0.001 ± 0.001
Pro	5.502ProAla: 5.502 ± 0.071	0.343ProCys: 0.343 ± 0.015	3.203ProAsp: 3.203 ± 0.048	3.36ProGlu: 3.36 ± 0.05	1.811ProPhe: 1.811 ± 0.033	3.892ProGly: 3.892 ± 0.051	1.119ProHis: 1.119 ± 0.026	2.26ProIle: 2.26 ± 0.035	1.737ProLys: 1.737 ± 0.04	4.703ProLeu: 4.703 ± 0.067	1.184ProMet: 1.184 ± 0.027	1.514ProAsn: 1.514 ± 0.03	2.147ProPro: 2.147 ± 0.041	1.889ProGln: 1.889 ± 0.036	2.454ProArg: 2.454 ± 0.039	2.699ProSer: 2.699 ± 0.041	2.422ProThr: 2.422 ± 0.047	4.22ProVal: 4.22 ± 0.056	0.604ProTrp: 0.604 ± 0.023	1.266ProTyr: 1.266 ± 0.028	0.001ProXaa: 0.001 ± 0.001
Gln	4.639GlnAla: 4.639 ± 0.063	0.333GlnCys: 0.333 ± 0.016	1.667GlnAsp: 1.667 ± 0.037	2.011GlnGlu: 2.011 ± 0.035	1.393GlnPhe: 1.393 ± 0.027	2.621GlnGly: 2.621 ± 0.046	0.98GlnHis: 0.98 ± 0.027	2.072GlnIle: 2.072 ± 0.037	1.528GlnLys: 1.528 ± 0.038	4.004GlnLeu: 4.004 ± 0.05	1.044GlnMet: 1.044 ± 0.024	1.115GlnAsn: 1.115 ± 0.028	1.958GlnPro: 1.958 ± 0.034	2.044GlnGln: 2.044 ± 0.044	2.838GlnArg: 2.838 ± 0.049	2.184GlnSer: 2.184 ± 0.036	1.915GlnThr: 1.915 ± 0.039	2.906GlnVal: 2.906 ± 0.045	0.542GlnTrp: 0.542 ± 0.018	0.939GlnTyr: 0.939 ± 0.026	0.003GlnXaa: 0.003 ± 0.001
Arg	6.183ArgAla: 6.183 ± 0.085	0.548ArgCys: 0.548 ± 0.019	3.453ArgAsp: 3.453 ± 0.051	3.981ArgGlu: 3.981 ± 0.052	2.681ArgPhe: 2.681 ± 0.034	3.941ArgGly: 3.941 ± 0.05	1.748ArgHis: 1.748 ± 0.039	3.886ArgIle: 3.886 ± 0.056	2.628ArgLys: 2.628 ± 0.051	6.947ArgLeu: 6.947 ± 0.087	1.954ArgMet: 1.954 ± 0.032	2.195ArgAsn: 2.195 ± 0.043	2.709ArgPro: 2.709 ± 0.042	2.743ArgGln: 2.743 ± 0.038	4.291ArgArg: 4.291 ± 0.05	3.47ArgSer: 3.47 ± 0.046	3.245ArgThr: 3.245 ± 0.041	4.365ArgVal: 4.365 ± 0.057	0.896ArgTrp: 0.896 ± 0.024	1.924ArgTyr: 1.924 ± 0.037	0.002ArgXaa: 0.002 ± 0.001
Ser	6.363SerAla: 6.363 ± 0.066	0.48SerCys: 0.48 ± 0.016	3.065SerAsp: 3.065 ± 0.048	3.072SerGlu: 3.072 ± 0.049	2.315SerPhe: 2.315 ± 0.034	5.408SerGly: 5.408 ± 0.06	1.312SerHis: 1.312 ± 0.028	3.08SerIle: 3.08 ± 0.041	2.1SerLys: 2.1 ± 0.042	5.945SerLeu: 5.945 ± 0.071	1.622SerMet: 1.622 ± 0.034	1.793SerAsn: 1.793 ± 0.038	2.812SerPro: 2.812 ± 0.046	2.065SerGln: 2.065 ± 0.036	3.656SerArg: 3.656 ± 0.05	3.582SerSer: 3.582 ± 0.055	3.205SerThr: 3.205 ± 0.053	4.262SerVal: 4.262 ± 0.05	0.716SerTrp: 0.716 ± 0.021	1.433SerTyr: 1.433 ± 0.027	0.002SerXaa: 0.002 ± 0.001
Thr	5.841ThrAla: 5.841 ± 0.065	0.446ThrCys: 0.446 ± 0.017	2.781ThrAsp: 2.781 ± 0.046	2.752ThrGlu: 2.752 ± 0.049	1.859ThrPhe: 1.859 ± 0.036	4.822ThrGly: 4.822 ± 0.069	1.215ThrHis: 1.215 ± 0.027	2.84ThrIle: 2.84 ± 0.05	1.657ThrLys: 1.657 ± 0.033	6.047ThrLeu: 6.047 ± 0.066	1.38ThrMet: 1.38 ± 0.03	1.533ThrAsn: 1.533 ± 0.039	3.231ThrPro: 3.231 ± 0.054	1.872ThrGln: 1.872 ± 0.037	3.187ThrArg: 3.187 ± 0.046	3.186ThrSer: 3.186 ± 0.054	3.093ThrThr: 3.093 ± 0.06	4.375ThrVal: 4.375 ± 0.061	0.697ThrTrp: 0.697 ± 0.022	1.296ThrTyr: 1.296 ± 0.03	0.003ThrXaa: 0.003 ± 0.001
Val	8.4ValAla: 8.4 ± 0.071	0.781ValCys: 0.781 ± 0.02	4.075ValAsp: 4.075 ± 0.05	4.233ValGlu: 4.233 ± 0.053	2.714ValPhe: 2.714 ± 0.046	5.207ValGly: 5.207 ± 0.058	1.597ValHis: 1.597 ± 0.035	4.132ValIle: 4.132 ± 0.059	2.91ValLys: 2.91 ± 0.049	7.608ValLeu: 7.608 ± 0.076	2.034ValMet: 2.034 ± 0.033	2.416ValAsn: 2.416 ± 0.04	3.762ValPro: 3.762 ± 0.046	2.587ValGln: 2.587 ± 0.046	4.678ValArg: 4.678 ± 0.06	4.561ValSer: 4.561 ± 0.059	4.336ValThr: 4.336 ± 0.055	5.919ValVal: 5.919 ± 0.069	0.858ValTrp: 0.858 ± 0.022	1.634ValTyr: 1.634 ± 0.031	0.003ValXaa: 0.003 ± 0.001
Trp	1.034TrpAla: 1.034 ± 0.026	0.148TrpCys: 0.148 ± 0.008	0.585TrpAsp: 0.585 ± 0.021	0.579TrpGlu: 0.579 ± 0.022	0.559TrpPhe: 0.559 ± 0.018	0.781TrpGly: 0.781 ± 0.026	0.377TrpHis: 0.377 ± 0.015	0.751TrpIle: 0.751 ± 0.02	0.556TrpLys: 0.556 ± 0.017	1.626TrpLeu: 1.626 ± 0.035	0.389TrpMet: 0.389 ± 0.017	0.493TrpAsn: 0.493 ± 0.02	0.558TrpPro: 0.558 ± 0.019	0.654TrpGln: 0.654 ± 0.022	0.902TrpArg: 0.902 ± 0.023	0.733TrpSer: 0.733 ± 0.022	0.671TrpThr: 0.671 ± 0.021	0.822TrpVal: 0.822 ± 0.022	0.203TrpTrp: 0.203 ± 0.011	0.308TrpTyr: 0.308 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.68TyrAla: 2.68 ± 0.035	0.288TyrCys: 0.288 ± 0.013	1.348TyrAsp: 1.348 ± 0.029	1.258TyrGlu: 1.258 ± 0.028	1.084TyrPhe: 1.084 ± 0.025	2.142TyrGly: 2.142 ± 0.037	0.539TyrHis: 0.539 ± 0.019	1.097TyrIle: 1.097 ± 0.024	0.887TyrLys: 0.887 ± 0.023	2.693TyrLeu: 2.693 ± 0.043	0.552TyrMet: 0.552 ± 0.018	0.681TyrAsn: 0.681 ± 0.021	1.283TyrPro: 1.283 ± 0.028	1.035TyrGln: 1.035 ± 0.026	1.901TyrArg: 1.901 ± 0.036	1.388TyrSer: 1.388 ± 0.03	1.318TyrThr: 1.318 ± 0.034	1.74TyrVal: 1.74 ± 0.033	0.381TyrTrp: 0.381 ± 0.016	0.708TyrTyr: 0.708 ± 0.022	0.002TyrXaa: 0.002 ± 0.001
Xaa	0.005XaaAla: 0.005 ± 0.002	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.004XaaGly: 0.004 ± 0.002	0.002XaaHis: 0.002 ± 0.001	0.002XaaIle: 0.002 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.004XaaLeu: 0.004 ± 0.002	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.006XaaArg: 0.006 ± 0.002	0.005XaaSer: 0.005 ± 0.002	0.001XaaThr: 0.001 ± 0.001	0.003XaaVal: 0.003 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.001	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 5322 proteins (1677877 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---