Amino acid dipepetide frequency for Staphylococcus piscifermentans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.643AlaAla: 5.643 ± 0.161	0.533AlaCys: 0.533 ± 0.031	3.95AlaAsp: 3.95 ± 0.147	4.944AlaGlu: 4.944 ± 0.114	3.196AlaPhe: 3.196 ± 0.078	4.837AlaGly: 4.837 ± 0.125	1.569AlaHis: 1.569 ± 0.053	5.95AlaIle: 5.95 ± 0.105	4.803AlaLys: 4.803 ± 0.178	7.4AlaLeu: 7.4 ± 0.192	1.979AlaMet: 1.979 ± 0.057	2.765AlaAsn: 2.765 ± 0.072	2.116AlaPro: 2.116 ± 0.054	2.96AlaGln: 2.96 ± 0.081	2.388AlaArg: 2.388 ± 0.061	3.981AlaSer: 3.981 ± 0.094	3.628AlaThr: 3.628 ± 0.104	5.208AlaVal: 5.208 ± 0.12	0.484AlaTrp: 0.484 ± 0.025	2.454AlaTyr: 2.454 ± 0.061	0.0AlaXaa: 0.0 ± 0.0
Cys	0.364CysAla: 0.364 ± 0.022	0.074CysCys: 0.074 ± 0.012	0.361CysAsp: 0.361 ± 0.021	0.345CysGlu: 0.345 ± 0.025	0.251CysPhe: 0.251 ± 0.021	0.64CysGly: 0.64 ± 0.028	0.148CysHis: 0.148 ± 0.015	0.501CysIle: 0.501 ± 0.023	0.275CysLys: 0.275 ± 0.02	0.51CysLeu: 0.51 ± 0.028	0.147CysMet: 0.147 ± 0.014	0.237CysAsn: 0.237 ± 0.018	0.284CysPro: 0.284 ± 0.019	0.16CysGln: 0.16 ± 0.015	0.253CysArg: 0.253 ± 0.022	0.364CysSer: 0.364 ± 0.024	0.342CysThr: 0.342 ± 0.022	0.349CysVal: 0.349 ± 0.023	0.053CysTrp: 0.053 ± 0.009	0.256CysTyr: 0.256 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	4.319AspAla: 4.319 ± 0.23	0.317AspCys: 0.317 ± 0.021	3.119AspAsp: 3.119 ± 0.078	4.901AspGlu: 4.901 ± 0.1	2.687AspPhe: 2.687 ± 0.068	3.622AspGly: 3.622 ± 0.258	1.187AspHis: 1.187 ± 0.04	4.814AspIle: 4.814 ± 0.095	4.927AspLys: 4.927 ± 0.156	5.194AspLeu: 5.194 ± 0.108	1.518AspMet: 1.518 ± 0.047	2.853AspAsn: 2.853 ± 0.073	1.689AspPro: 1.689 ± 0.06	2.081AspGln: 2.081 ± 0.067	1.977AspArg: 1.977 ± 0.053	2.858AspSer: 2.858 ± 0.075	3.102AspThr: 3.102 ± 0.086	4.033AspVal: 4.033 ± 0.087	0.501AspTrp: 0.501 ± 0.028	2.873AspTyr: 2.873 ± 0.074	0.0AspXaa: 0.0 ± 0.0
Glu	5.623GluAla: 5.623 ± 0.121	0.249GluCys: 0.249 ± 0.02	4.29GluAsp: 4.29 ± 0.095	5.556GluGlu: 5.556 ± 0.125	2.396GluPhe: 2.396 ± 0.06	3.93GluGly: 3.93 ± 0.089	1.727GluHis: 1.727 ± 0.052	5.467GluIle: 5.467 ± 0.092	5.792GluLys: 5.792 ± 0.105	6.198GluLeu: 6.198 ± 0.128	2.181GluMet: 2.181 ± 0.065	3.793GluAsn: 3.793 ± 0.076	1.922GluPro: 1.922 ± 0.062	3.175GluGln: 3.175 ± 0.071	3.274GluArg: 3.274 ± 0.072	3.404GluSer: 3.404 ± 0.072	3.992GluThr: 3.992 ± 0.078	5.01GluVal: 5.01 ± 0.089	0.607GluTrp: 0.607 ± 0.031	2.344GluTyr: 2.344 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	2.956PheAla: 2.956 ± 0.075	0.312PheCys: 0.312 ± 0.021	2.735PheAsp: 2.735 ± 0.066	3.029PheGlu: 3.029 ± 0.07	2.16PhePhe: 2.16 ± 0.072	3.239PheGly: 3.239 ± 0.079	0.88PheHis: 0.88 ± 0.039	4.09PheIle: 4.09 ± 0.103	3.333PheLys: 3.333 ± 0.083	3.879PheLeu: 3.879 ± 0.104	1.192PheMet: 1.192 ± 0.044	2.481PheAsn: 2.481 ± 0.062	1.355PhePro: 1.355 ± 0.045	1.3PheGln: 1.3 ± 0.044	1.309PheArg: 1.309 ± 0.041	2.955PheSer: 2.955 ± 0.118	2.436PheThr: 2.436 ± 0.06	3.034PheVal: 3.034 ± 0.087	0.36PheTrp: 0.36 ± 0.024	1.631PheTyr: 1.631 ± 0.055	0.0PheXaa: 0.0 ± 0.0
Gly	4.722GlyAla: 4.722 ± 0.147	0.444GlyCys: 0.444 ± 0.026	3.362GlyAsp: 3.362 ± 0.19	3.929GlyGlu: 3.929 ± 0.09	3.149GlyPhe: 3.149 ± 0.08	4.478GlyGly: 4.478 ± 0.11	1.545GlyHis: 1.545 ± 0.05	5.594GlyIle: 5.594 ± 0.106	4.694GlyLys: 4.694 ± 0.128	6.115GlyLeu: 6.115 ± 0.099	1.821GlyMet: 1.821 ± 0.047	2.783GlyAsn: 2.783 ± 0.101	1.739GlyPro: 1.739 ± 0.052	2.453GlyGln: 2.453 ± 0.094	2.389GlyArg: 2.389 ± 0.066	3.68GlySer: 3.68 ± 0.069	3.79GlyThr: 3.79 ± 0.091	4.631GlyVal: 4.631 ± 0.084	0.564GlyTrp: 0.564 ± 0.031	2.749GlyTyr: 2.749 ± 0.063	0.0GlyXaa: 0.0 ± 0.0
His	1.479HisAla: 1.479 ± 0.051	0.159HisCys: 0.159 ± 0.016	1.284HisAsp: 1.284 ± 0.045	1.403HisGlu: 1.403 ± 0.042	1.223HisPhe: 1.223 ± 0.043	1.456HisGly: 1.456 ± 0.049	0.803HisHis: 0.803 ± 0.041	1.712HisIle: 1.712 ± 0.056	1.277HisLys: 1.277 ± 0.043	2.251HisLeu: 2.251 ± 0.067	0.576HisMet: 0.576 ± 0.031	1.071HisAsn: 1.071 ± 0.04	1.27HisPro: 1.27 ± 0.045	1.007HisGln: 1.007 ± 0.037	0.951HisArg: 0.951 ± 0.038	1.253HisSer: 1.253 ± 0.048	1.173HisThr: 1.173 ± 0.041	1.421HisVal: 1.421 ± 0.039	0.203HisTrp: 0.203 ± 0.017	1.117HisTyr: 1.117 ± 0.042	0.0HisXaa: 0.0 ± 0.0
Ile	6.095IleAla: 6.095 ± 0.124	0.598IleCys: 0.598 ± 0.026	4.869IleAsp: 4.869 ± 0.084	5.629IleGlu: 5.629 ± 0.107	3.634IlePhe: 3.634 ± 0.111	5.934IleGly: 5.934 ± 0.114	1.636IleHis: 1.636 ± 0.048	6.837IleIle: 6.837 ± 0.15	5.138IleLys: 5.138 ± 0.09	7.3IleLeu: 7.3 ± 0.159	2.012IleMet: 2.012 ± 0.066	4.011IleAsn: 4.011 ± 0.085	3.216IlePro: 3.216 ± 0.092	3.025IleGln: 3.025 ± 0.078	2.618IleArg: 2.618 ± 0.062	4.756IleSer: 4.756 ± 0.096	4.54IleThr: 4.54 ± 0.117	5.844IleVal: 5.844 ± 0.101	0.551IleTrp: 0.551 ± 0.031	2.719IleTyr: 2.719 ± 0.067	0.0IleXaa: 0.0 ± 0.0
Lys	5.507LysAla: 5.507 ± 0.277	0.272LysCys: 0.272 ± 0.021	5.198LysAsp: 5.198 ± 0.13	6.426LysGlu: 6.426 ± 0.12	2.31LysPhe: 2.31 ± 0.061	4.375LysGly: 4.375 ± 0.228	1.702LysHis: 1.702 ± 0.051	4.647LysIle: 4.647 ± 0.089	5.6LysLys: 5.6 ± 0.131	5.785LysLeu: 5.785 ± 0.1	2.108LysMet: 2.108 ± 0.052	3.585LysAsn: 3.585 ± 0.088	2.318LysPro: 2.318 ± 0.06	3.397LysGln: 3.397 ± 0.08	3.095LysArg: 3.095 ± 0.067	3.744LysSer: 3.744 ± 0.083	3.564LysThr: 3.564 ± 0.086	5.067LysVal: 5.067 ± 0.112	0.603LysTrp: 0.603 ± 0.025	2.668LysTyr: 2.668 ± 0.063	0.0LysXaa: 0.0 ± 0.0
Leu	6.344LeuAla: 6.344 ± 0.118	0.564LeuCys: 0.564 ± 0.028	5.23LeuAsp: 5.23 ± 0.119	5.937LeuGlu: 5.937 ± 0.114	4.379LeuPhe: 4.379 ± 0.113	5.93LeuGly: 5.93 ± 0.101	1.834LeuHis: 1.834 ± 0.053	7.703LeuIle: 7.703 ± 0.148	6.888LeuLys: 6.888 ± 0.121	8.699LeuLeu: 8.699 ± 0.178	2.457LeuMet: 2.457 ± 0.064	5.195LeuAsn: 5.195 ± 0.102	3.678LeuPro: 3.678 ± 0.069	3.034LeuGln: 3.034 ± 0.07	3.113LeuArg: 3.113 ± 0.074	6.313LeuSer: 6.313 ± 0.143	5.46LeuThr: 5.46 ± 0.094	5.973LeuVal: 5.973 ± 0.117	0.646LeuTrp: 0.646 ± 0.032	3.037LeuTyr: 3.037 ± 0.077	0.0LeuXaa: 0.0 ± 0.0
Met	1.867MetAla: 1.867 ± 0.06	0.121MetCys: 0.121 ± 0.013	1.398MetAsp: 1.398 ± 0.051	1.511MetGlu: 1.511 ± 0.049	1.106MetPhe: 1.106 ± 0.042	1.533MetGly: 1.533 ± 0.048	0.59MetHis: 0.59 ± 0.03	2.213MetIle: 2.213 ± 0.068	2.272MetLys: 2.272 ± 0.059	2.459MetLeu: 2.459 ± 0.075	0.936MetMet: 0.936 ± 0.042	1.631MetAsn: 1.631 ± 0.055	1.071MetPro: 1.071 ± 0.042	1.033MetGln: 1.033 ± 0.04	1.055MetArg: 1.055 ± 0.04	1.845MetSer: 1.845 ± 0.05	1.743MetThr: 1.743 ± 0.053	1.556MetVal: 1.556 ± 0.053	0.145MetTrp: 0.145 ± 0.015	0.832MetTyr: 0.832 ± 0.035	0.0MetXaa: 0.0 ± 0.0
Asn	3.313AsnAla: 3.313 ± 0.094	0.253AsnCys: 0.253 ± 0.016	2.907AsnAsp: 2.907 ± 0.072	3.681AsnGlu: 3.681 ± 0.079	2.015AsnPhe: 2.015 ± 0.054	3.092AsnGly: 3.092 ± 0.092	1.432AsnHis: 1.432 ± 0.048	3.953AsnIle: 3.953 ± 0.08	3.759AsnLys: 3.759 ± 0.093	4.129AsnLeu: 4.129 ± 0.085	1.226AsnMet: 1.226 ± 0.042	2.707AsnAsn: 2.707 ± 0.087	2.203AsnPro: 2.203 ± 0.055	2.7AsnGln: 2.7 ± 0.076	1.968AsnArg: 1.968 ± 0.054	2.373AsnSer: 2.373 ± 0.07	2.616AsnThr: 2.616 ± 0.107	3.265AsnVal: 3.265 ± 0.06	0.404AsnTrp: 0.404 ± 0.024	2.015AsnTyr: 2.015 ± 0.054	0.0AsnXaa: 0.0 ± 0.0
Pro	2.19ProAla: 2.19 ± 0.066	0.187ProCys: 0.187 ± 0.013	2.218ProAsp: 2.218 ± 0.084	3.146ProGlu: 3.146 ± 0.066	1.899ProPhe: 1.899 ± 0.055	2.12ProGly: 2.12 ± 0.063	0.818ProHis: 0.818 ± 0.03	2.722ProIle: 2.722 ± 0.061	2.528ProLys: 2.528 ± 0.098	3.265ProLeu: 3.265 ± 0.073	0.839ProMet: 0.839 ± 0.033	1.849ProAsn: 1.849 ± 0.065	0.842ProPro: 0.842 ± 0.032	1.222ProGln: 1.222 ± 0.043	1.029ProArg: 1.029 ± 0.038	2.12ProSer: 2.12 ± 0.061	1.961ProThr: 1.961 ± 0.059	2.633ProVal: 2.633 ± 0.085	0.31ProTrp: 0.31 ± 0.023	1.436ProTyr: 1.436 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	2.933GlnAla: 2.933 ± 0.095	0.182GlnCys: 0.182 ± 0.015	2.164GlnAsp: 2.164 ± 0.072	2.492GlnGlu: 2.492 ± 0.068	1.859GlnPhe: 1.859 ± 0.055	2.108GlnGly: 2.108 ± 0.062	1.098GlnHis: 1.098 ± 0.04	2.853GlnIle: 2.853 ± 0.072	2.71GlnLys: 2.71 ± 0.066	3.956GlnLeu: 3.956 ± 0.085	1.133GlnMet: 1.133 ± 0.041	2.194GlnAsn: 2.194 ± 0.062	1.623GlnPro: 1.623 ± 0.062	2.159GlnGln: 2.159 ± 0.085	1.667GlnArg: 1.667 ± 0.05	2.467GlnSer: 2.467 ± 0.065	2.358GlnThr: 2.358 ± 0.069	2.299GlnVal: 2.299 ± 0.055	0.342GlnTrp: 0.342 ± 0.023	1.69GlnTyr: 1.69 ± 0.061	0.0GlnXaa: 0.0 ± 0.0
Arg	2.265ArgAla: 2.265 ± 0.062	0.211ArgCys: 0.211 ± 0.025	2.024ArgAsp: 2.024 ± 0.058	2.75ArgGlu: 2.75 ± 0.072	1.798ArgPhe: 1.798 ± 0.051	2.084ArgGly: 2.084 ± 0.059	0.912ArgHis: 0.912 ± 0.035	2.978ArgIle: 2.978 ± 0.062	2.843ArgLys: 2.843 ± 0.085	3.519ArgLeu: 3.519 ± 0.073	1.048ArgMet: 1.048 ± 0.041	1.957ArgAsn: 1.957 ± 0.061	1.254ArgPro: 1.254 ± 0.04	1.604ArgGln: 1.604 ± 0.053	1.662ArgArg: 1.662 ± 0.055	1.724ArgSer: 1.724 ± 0.049	1.939ArgThr: 1.939 ± 0.051	2.37ArgVal: 2.37 ± 0.057	0.291ArgTrp: 0.291 ± 0.022	1.581ArgTyr: 1.581 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	3.642SerAla: 3.642 ± 0.085	0.311SerCys: 0.311 ± 0.024	3.463SerAsp: 3.463 ± 0.131	4.09SerGlu: 4.09 ± 0.085	2.714SerPhe: 2.714 ± 0.07	4.008SerGly: 4.008 ± 0.076	1.331SerHis: 1.331 ± 0.049	4.781SerIle: 4.781 ± 0.108	4.123SerLys: 4.123 ± 0.086	5.309SerLeu: 5.309 ± 0.104	1.49SerMet: 1.49 ± 0.049	2.795SerAsn: 2.795 ± 0.071	1.911SerPro: 1.911 ± 0.057	2.291SerGln: 2.291 ± 0.061	2.19SerArg: 2.19 ± 0.078	3.369SerSer: 3.369 ± 0.07	3.086SerThr: 3.086 ± 0.077	3.682SerVal: 3.682 ± 0.079	0.453SerTrp: 0.453 ± 0.027	2.261SerTyr: 2.261 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	4.039ThrAla: 4.039 ± 0.114	0.327ThrCys: 0.327 ± 0.022	3.195ThrAsp: 3.195 ± 0.077	3.623ThrGlu: 3.623 ± 0.079	2.645ThrPhe: 2.645 ± 0.071	3.921ThrGly: 3.921 ± 0.098	1.366ThrHis: 1.366 ± 0.047	4.466ThrIle: 4.466 ± 0.104	3.428ThrLys: 3.428 ± 0.088	5.261ThrLeu: 5.261 ± 0.088	1.266ThrMet: 1.266 ± 0.042	2.411ThrAsn: 2.411 ± 0.065	2.582ThrPro: 2.582 ± 0.094	2.066ThrGln: 2.066 ± 0.064	1.743ThrArg: 1.743 ± 0.047	3.324ThrSer: 3.324 ± 0.087	3.134ThrThr: 3.134 ± 0.105	4.178ThrVal: 4.178 ± 0.147	0.45ThrTrp: 0.45 ± 0.025	2.042ThrTyr: 2.042 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	4.675ValAla: 4.675 ± 0.092	0.501ValCys: 0.501 ± 0.025	4.014ValAsp: 4.014 ± 0.081	4.668ValGlu: 4.668 ± 0.08	2.835ValPhe: 2.835 ± 0.069	4.396ValGly: 4.396 ± 0.083	1.288ValHis: 1.288 ± 0.045	6.003ValIle: 6.003 ± 0.114	4.796ValLys: 4.796 ± 0.091	6.505ValLeu: 6.505 ± 0.141	1.818ValMet: 1.818 ± 0.056	3.373ValAsn: 3.373 ± 0.076	2.591ValPro: 2.591 ± 0.069	2.445ValGln: 2.445 ± 0.074	2.252ValArg: 2.252 ± 0.06	4.286ValSer: 4.286 ± 0.07	4.092ValThr: 4.092 ± 0.152	4.975ValVal: 4.975 ± 0.1	0.477ValTrp: 0.477 ± 0.027	2.245ValTyr: 2.245 ± 0.062	0.0ValXaa: 0.0 ± 0.0
Trp	0.431TrpAla: 0.431 ± 0.027	0.055TrpCys: 0.055 ± 0.008	0.401TrpAsp: 0.401 ± 0.027	0.373TrpGlu: 0.373 ± 0.02	0.474TrpPhe: 0.474 ± 0.028	0.467TrpGly: 0.467 ± 0.025	0.187TrpHis: 0.187 ± 0.016	0.748TrpIle: 0.748 ± 0.036	0.477TrpLys: 0.477 ± 0.026	0.928TrpLeu: 0.928 ± 0.043	0.311TrpMet: 0.311 ± 0.023	0.416TrpAsn: 0.416 ± 0.026	0.214TrpPro: 0.214 ± 0.021	0.296TrpGln: 0.296 ± 0.02	0.31TrpArg: 0.31 ± 0.018	0.461TrpSer: 0.461 ± 0.029	0.418TrpThr: 0.418 ± 0.024	0.479TrpVal: 0.479 ± 0.028	0.086TrpTrp: 0.086 ± 0.01	0.307TrpTyr: 0.307 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.381TyrAla: 2.381 ± 0.06	0.24TyrCys: 0.24 ± 0.018	2.388TyrAsp: 2.388 ± 0.06	2.496TyrGlu: 2.496 ± 0.075	1.939TyrPhe: 1.939 ± 0.067	2.459TyrGly: 2.459 ± 0.062	1.037TyrHis: 1.037 ± 0.04	2.932TyrIle: 2.932 ± 0.074	2.309TyrLys: 2.309 ± 0.062	3.752TyrLeu: 3.752 ± 0.094	0.859TyrMet: 0.859 ± 0.038	1.906TyrAsn: 1.906 ± 0.047	1.455TyrPro: 1.455 ± 0.047	1.879TyrGln: 1.879 ± 0.055	1.573TyrArg: 1.573 ± 0.05	2.024TyrSer: 2.024 ± 0.058	2.092TyrThr: 2.092 ± 0.053	2.226TyrVal: 2.226 ± 0.059	0.31TyrTrp: 0.31 ± 0.022	1.597TyrTyr: 1.597 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2389 proteins (742465 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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