Actinobaculum sp. 352
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2387 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A430JR66|A0A430JR66_9ACTO Non-specific serine/threonine protein kinase OS=Actinobaculum sp. 352 OX=2490946 GN=EKN07_06770 PE=4 SV=1
MM1 pKa = 7.26 IWPWHH6 pKa = 4.87 TTPAQAAEE14 pKa = 4.27 VTVPLGEE21 pKa = 4.41 TVASVSNVTMKK32 pKa = 10.67 EE33 pKa = 3.86 EE34 pKa = 4.93 PIGGGYY40 pKa = 9.71 SNEE43 pKa = 4.22 YY44 pKa = 8.14 YY45 pKa = 10.72 ANRR48 pKa = 11.84 YY49 pKa = 8.9 GGVRR53 pKa = 11.84 YY54 pKa = 10.34 SPVSGGNYY62 pKa = 10.03 LYY64 pKa = 9.38 QTTTEE69 pKa = 4.75 DD70 pKa = 3.38 VTGSQQEE77 pKa = 4.38 YY78 pKa = 11.16 ALFAYY83 pKa = 9.71 GWGTNQSSAGPTADD97 pKa = 4.32 LQTQSSVGFRR107 pKa = 11.84 PGEE110 pKa = 3.92 TSAVEE115 pKa = 3.95 VGKK118 pKa = 10.92 SFLVGDD124 pKa = 3.68 MRR126 pKa = 11.84 HH127 pKa = 5.28 WNNPIFTTTDD137 pKa = 2.5 SRR139 pKa = 11.84 YY140 pKa = 10.32 KK141 pKa = 10.93 DD142 pKa = 3.54 DD143 pKa = 5.25 DD144 pKa = 4.51 GNGGSLLYY152 pKa = 11.15 GNINVALGQNPDD164 pKa = 2.92 GTVAIDD170 pKa = 3.27 SAFPFTLHH178 pKa = 5.71 EE179 pKa = 4.33 TVNTCRR185 pKa = 11.84 STFDD189 pKa = 3.73 ADD191 pKa = 3.28 GNYY194 pKa = 9.57 VEE196 pKa = 5.76 DD197 pKa = 4.25 GSGRR201 pKa = 11.84 YY202 pKa = 9.5 AMNSAGQVGLRR213 pKa = 11.84 ATWTGWGWSSDD224 pKa = 3.26 YY225 pKa = 10.8 HH226 pKa = 5.95 YY227 pKa = 11.04 AYY229 pKa = 10.31 DD230 pKa = 3.28 RR231 pKa = 11.84 SGILRR236 pKa = 11.84 SFPAGDD242 pKa = 3.17 VAAAGEE248 pKa = 4.52 DD249 pKa = 3.26 NGALYY254 pKa = 10.34 GKK256 pKa = 9.8 NGRR259 pKa = 11.84 SCEE262 pKa = 3.94 DD263 pKa = 3.78 DD264 pKa = 3.94 YY265 pKa = 11.81 LTIATDD271 pKa = 3.62 RR272 pKa = 11.84 SDD274 pKa = 3.95 TAWTDD279 pKa = 3.22 PTTGIQYY286 pKa = 10.87 KK287 pKa = 10.03 LKK289 pKa = 10.75 LWGFVNKK296 pKa = 10.16 GADD299 pKa = 3.34 WQACAAEE306 pKa = 4.83 LDD308 pKa = 3.86 EE309 pKa = 6.67 ADD311 pKa = 4.72 IVDD314 pKa = 4.56 LEE316 pKa = 4.48 AGFVTKK322 pKa = 10.37 EE323 pKa = 3.28 AAQTYY328 pKa = 8.02 GCLYY332 pKa = 10.9 GSMEE336 pKa = 4.18 QVRR339 pKa = 11.84 EE340 pKa = 4.09 VTFQKK345 pKa = 10.86 YY346 pKa = 7.46 ATTAEE351 pKa = 3.96 QWQDD355 pKa = 3.19 TEE357 pKa = 4.84 IPTFTYY363 pKa = 11.04 SNLSEE368 pKa = 4.36 VGSVAAEE375 pKa = 4.01 TWSPMGSMTPSGWGTTNMAVDD396 pKa = 3.74 SKK398 pKa = 9.82 TYY400 pKa = 10.62 EE401 pKa = 4.09 LLAPDD406 pKa = 4.85 DD407 pKa = 3.84 RR408 pKa = 11.84 AKK410 pKa = 10.87 VQEE413 pKa = 4.32 DD414 pKa = 4.64 TPVPALTDD422 pKa = 3.51 SSSGWRR428 pKa = 11.84 LADD431 pKa = 3.34 IVCTYY436 pKa = 11.28 GDD438 pKa = 4.75 DD439 pKa = 4.69 GNILLDD445 pKa = 3.78 RR446 pKa = 11.84 NGNPFDD452 pKa = 3.87 STADD456 pKa = 3.47 NPTPNIDD463 pKa = 3.2 IDD465 pKa = 4.05 AGTLALDD472 pKa = 3.91 STDD475 pKa = 4.4 LADD478 pKa = 5.07 NVNDD482 pKa = 3.91 TNITCTYY489 pKa = 8.57 HH490 pKa = 7.15 NEE492 pKa = 3.96 YY493 pKa = 9.39 EE494 pKa = 4.35 AKK496 pKa = 10.39 SRR498 pKa = 11.84 LTLVKK503 pKa = 10.12 ALQGDD508 pKa = 3.95 VPDD511 pKa = 5.65 GVTADD516 pKa = 3.28 QWTVTANGTDD526 pKa = 3.14 TMTSDD531 pKa = 3.43 TAVSGRR537 pKa = 11.84 SGSEE541 pKa = 3.48 AVTSQVVPSGSYY553 pKa = 9.65 QLSEE557 pKa = 4.16 SAPADD562 pKa = 3.36 VYY564 pKa = 11.34 GFEE567 pKa = 5.32 LVEE570 pKa = 4.58 DD571 pKa = 4.64 WSCADD576 pKa = 3.76 ADD578 pKa = 4.11 GATVDD583 pKa = 3.94 VSDD586 pKa = 4.83 SVVNLADD593 pKa = 4.6 DD594 pKa = 4.78 ADD596 pKa = 4.28 VTCTVTNQLRR606 pKa = 11.84 TGSLQISKK614 pKa = 10.6 EE615 pKa = 3.58 IDD617 pKa = 3.52 DD618 pKa = 4.72 PLGGLADD625 pKa = 3.66 TTKK628 pKa = 10.35 TYY630 pKa = 10.54 DD631 pKa = 2.83 ISYY634 pKa = 10.89 DD635 pKa = 3.44 CGTANGVDD643 pKa = 3.68 YY644 pKa = 11.26 SGTRR648 pKa = 11.84 TVSAGEE654 pKa = 4.02 TVQIDD659 pKa = 4.82 GIPAGLTCTVSEE671 pKa = 4.2 EE672 pKa = 4.43 TPDD675 pKa = 3.57 GGLTNSSYY683 pKa = 10.8 HH684 pKa = 4.91 WAEE687 pKa = 4.09 PTFSAQDD694 pKa = 3.57 VVTVGDD700 pKa = 3.81 DD701 pKa = 3.71 TVSVTVTNHH710 pKa = 5.85 IAQEE714 pKa = 4.12 TGLLQISKK722 pKa = 10.37 RR723 pKa = 11.84 VLPEE727 pKa = 4.64 DD728 pKa = 4.66 GVAQPGYY735 pKa = 7.86 TGEE738 pKa = 4.0 RR739 pKa = 11.84 AFPIDD744 pKa = 4.15 YY745 pKa = 10.43 SCQLDD750 pKa = 3.87 GSGVASGTVEE760 pKa = 4.01 LADD763 pKa = 4.16 GEE765 pKa = 4.9 STTVTVPAGATCTTSEE781 pKa = 4.44 DD782 pKa = 3.84 VPTVEE787 pKa = 5.91 DD788 pKa = 4.24 GDD790 pKa = 4.28 FATPAYY796 pKa = 9.53 EE797 pKa = 3.77 WTRR800 pKa = 11.84 NNIVDD805 pKa = 3.62 QTVTVAADD813 pKa = 3.48 ATASTTVEE821 pKa = 3.57 NYY823 pKa = 7.0 YY824 pKa = 9.89 TVVLVPLNLTKK835 pKa = 10.5 QVVGGGYY842 pKa = 10.33 LGTAADD848 pKa = 4.43 GSTHH852 pKa = 6.46 TFTITADD859 pKa = 3.3 CGAGFTYY866 pKa = 10.22 TEE868 pKa = 4.19 EE869 pKa = 4.06 VAADD873 pKa = 3.59 EE874 pKa = 4.61 TVTVQVPRR882 pKa = 11.84 GTTCQISEE890 pKa = 4.31 NADD893 pKa = 3.35 GADD896 pKa = 4.52 DD897 pKa = 5.56 DD898 pKa = 5.03 FADD901 pKa = 4.44 DD902 pKa = 5.06 VLDD905 pKa = 5.87 ADD907 pKa = 5.32 HH908 pKa = 6.98 EE909 pKa = 4.36 WDD911 pKa = 3.65 PAATEE916 pKa = 4.12 YY917 pKa = 11.54 VDD919 pKa = 5.44 ANGQALEE926 pKa = 4.28 NNLIAISSAGGEE938 pKa = 4.0 TAKK941 pKa = 10.88 VINHH945 pKa = 5.65 TTAAWGGIAVTKK957 pKa = 9.18 VVPQTEE963 pKa = 4.29 GLVAGTAFDD972 pKa = 5.22 FDD974 pKa = 4.2 IACDD978 pKa = 3.8 APAQGDD984 pKa = 3.95 DD985 pKa = 3.86 EE986 pKa = 5.91 NYY988 pKa = 7.78 TAHH991 pKa = 7.1 ISLAVGEE998 pKa = 4.42 VWRR1001 pKa = 11.84 NTGAPLAAGTTCTVTEE1017 pKa = 4.25 EE1018 pKa = 4.52 SLTGTSTGLVDD1029 pKa = 4.88 SSYY1032 pKa = 10.74 RR1033 pKa = 11.84 WASTTIDD1040 pKa = 3.73 PDD1042 pKa = 4.0 DD1043 pKa = 4.18 GVGNTVEE1050 pKa = 4.2 VDD1052 pKa = 3.11 GTTATVVAHH1061 pKa = 6.74 AASSDD1066 pKa = 2.94 AGRR1069 pKa = 11.84 PYY1071 pKa = 10.77 PRR1073 pKa = 11.84 VTFTNTYY1080 pKa = 8.11 EE1081 pKa = 4.12 RR1082 pKa = 11.84 VYY1084 pKa = 10.42 STIGVEE1090 pKa = 4.79 KK1091 pKa = 10.33 ILNTLDD1097 pKa = 3.57 SDD1099 pKa = 4.18 PSVGTTTTFTGTWTCSYY1116 pKa = 10.94 GGGATEE1122 pKa = 5.6 VSGTWSRR1129 pKa = 11.84 TGAGAATLTVTSGDD1143 pKa = 3.45 AVLSEE1148 pKa = 4.27 GNTRR1152 pKa = 11.84 ASVLLGSNCSITEE1165 pKa = 4.33 DD1166 pKa = 3.78 DD1167 pKa = 4.72 PGYY1170 pKa = 9.61 PDD1172 pKa = 4.13 PANHH1176 pKa = 6.36 SFYY1179 pKa = 9.71 WQNRR1183 pKa = 11.84 QAGEE1187 pKa = 4.55 PITVSADD1194 pKa = 2.91 ASANTLTYY1202 pKa = 9.77 TNTVGKK1208 pKa = 9.63 YY1209 pKa = 9.57 LAGLTITKK1217 pKa = 8.5 TVNGPGAGEE1226 pKa = 4.89 GYY1228 pKa = 10.5 AAGSFTVHH1236 pKa = 6.0 YY1237 pKa = 9.05 EE1238 pKa = 4.04 CTSQAGHH1245 pKa = 5.64 TVSGDD1250 pKa = 3.29 VALSDD1255 pKa = 4.63 LSSQLVSDD1263 pKa = 4.37 VPAGSTCTVSEE1274 pKa = 4.54 SEE1276 pKa = 4.4 NLPTPIDD1283 pKa = 3.94 PYY1285 pKa = 11.11 RR1286 pKa = 11.84 WDD1288 pKa = 3.67 TPSFTVSGDD1297 pKa = 3.19 GVTGTTTSDD1306 pKa = 3.39 GAVTFTLPDD1315 pKa = 3.34 EE1316 pKa = 4.91 GEE1318 pKa = 4.17 PEE1320 pKa = 4.15 VGVEE1324 pKa = 4.16 VTNSLSARR1332 pKa = 11.84 TGSVTVAKK1340 pKa = 9.6 TVADD1344 pKa = 4.22 EE1345 pKa = 4.15 NKK1347 pKa = 9.87 QGYY1350 pKa = 7.35 TGTTFPVSLLCTPPGGDD1367 pKa = 3.18 AATVMGTADD1376 pKa = 4.36 LGDD1379 pKa = 4.16 GDD1381 pKa = 4.66 SVTFEE1386 pKa = 4.3 VPLGSTGCSVTEE1398 pKa = 3.86 ATISGGLQDD1407 pKa = 6.12 DD1408 pKa = 4.65 SYY1410 pKa = 12.33 AWGTPDD1416 pKa = 5.29 FSDD1419 pKa = 3.57 PLANVGEE1426 pKa = 4.49 GDD1428 pKa = 3.55 NGTLTVTNHH1437 pKa = 5.1 IEE1439 pKa = 3.97 RR1440 pKa = 11.84 RR1441 pKa = 11.84 FGTISLSKK1449 pKa = 10.33 RR1450 pKa = 11.84 ITGEE1454 pKa = 3.34 GDD1456 pKa = 2.71 AAAQGGAVTYY1466 pKa = 9.24 TGSFVCTHH1474 pKa = 6.92 AGDD1477 pKa = 4.09 SDD1479 pKa = 3.95 ISGTWSVTGAGVATVIATVNGTEE1502 pKa = 3.82 VDD1504 pKa = 3.9 LAADD1508 pKa = 3.68 NSVLPLGASCTPAEE1522 pKa = 4.95 DD1523 pKa = 4.41 PVNDD1527 pKa = 4.54 PPNAGDD1533 pKa = 3.78 PSYY1536 pKa = 11.57 VWGAAGDD1543 pKa = 3.97 SAAVSDD1549 pKa = 4.4 AVVAADD1555 pKa = 3.7 ITNEE1559 pKa = 3.84 MTVTNEE1565 pKa = 3.77 VVQEE1569 pKa = 4.05 TSTVHH1574 pKa = 5.73 ISKK1577 pKa = 10.31 EE1578 pKa = 4.28 VTGEE1582 pKa = 3.92 TAGLANADD1590 pKa = 3.27 QTFTVFATCTDD1601 pKa = 3.54 EE1602 pKa = 5.89 GNDD1605 pKa = 3.59 SFDD1608 pKa = 3.54 PRR1610 pKa = 11.84 VVEE1613 pKa = 3.95 LTNGQTADD1621 pKa = 3.94 FPRR1624 pKa = 11.84 TLPRR1628 pKa = 11.84 GWTCRR1633 pKa = 11.84 LSEE1636 pKa = 4.15 EE1637 pKa = 5.03 SLTQDD1642 pKa = 2.97 MLKK1645 pKa = 10.49 DD1646 pKa = 3.33 VSYY1649 pKa = 11.31 AWGTPVFEE1657 pKa = 4.21 VTIDD1661 pKa = 3.59 GVTTEE1666 pKa = 3.85 TDD1668 pKa = 3.43 TFVVEE1673 pKa = 4.26 GDD1675 pKa = 3.72 EE1676 pKa = 4.71 ISIKK1680 pKa = 10.34 VSNPITRR1687 pKa = 11.84 VRR1689 pKa = 11.84 GSLEE1693 pKa = 3.36 IRR1695 pKa = 11.84 KK1696 pKa = 9.04 EE1697 pKa = 4.03 LGATAAQDD1705 pKa = 4.28 GIVNDD1710 pKa = 3.89 GTAYY1714 pKa = 10.35 SGSYY1718 pKa = 7.72 TCSYY1722 pKa = 10.56 DD1723 pKa = 3.45 GEE1725 pKa = 4.68 EE1726 pKa = 4.25 IASGTWSVDD1735 pKa = 3.14 GAGTATLTTSDD1746 pKa = 4.03 GEE1748 pKa = 4.5 EE1749 pKa = 4.02 QGEE1752 pKa = 4.31 LPLTAVCTANEE1763 pKa = 4.03 DD1764 pKa = 3.98 TPADD1768 pKa = 3.53 SALRR1772 pKa = 11.84 DD1773 pKa = 3.65 TSYY1776 pKa = 9.84 TWLDD1780 pKa = 3.3 PVVTYY1785 pKa = 10.59 SGPNVEE1791 pKa = 4.94 SGATVEE1797 pKa = 4.44 ADD1799 pKa = 3.39 SPALITVTNDD1809 pKa = 2.59 ATRR1812 pKa = 11.84 VYY1814 pKa = 11.08 SDD1816 pKa = 3.42 LRR1818 pKa = 11.84 IEE1820 pKa = 4.56 KK1821 pKa = 9.65 IYY1823 pKa = 11.04 SGVDD1827 pKa = 3.15 GDD1829 pKa = 4.1 GTTAGLADD1837 pKa = 4.0 GAEE1840 pKa = 4.11 VLISYY1845 pKa = 7.65 TCVAADD1851 pKa = 3.89 GSTVEE1856 pKa = 4.54 GQTTLPASGGAIATPDD1872 pKa = 3.27 LTAARR1877 pKa = 11.84 IPAGSEE1883 pKa = 3.81 CSIQEE1888 pKa = 3.77 QTLTDD1893 pKa = 4.43 DD1894 pKa = 4.18 LLSDD1898 pKa = 5.14 KK1899 pKa = 11.03 SYY1901 pKa = 10.48 GWNTPVYY1908 pKa = 9.88 EE1909 pKa = 4.19 VTPDD1913 pKa = 3.19 EE1914 pKa = 4.66 GGEE1917 pKa = 3.97 FAGGNSVTTEE1927 pKa = 4.01 PEE1929 pKa = 3.36 ATAVLRR1935 pKa = 11.84 VTNEE1939 pKa = 3.39 TTRR1942 pKa = 11.84 RR1943 pKa = 11.84 YY1944 pKa = 9.76 GALEE1948 pKa = 3.82 IVKK1951 pKa = 9.63 DD1952 pKa = 3.64 IPEE1955 pKa = 4.48 GSTATFGNVYY1965 pKa = 10.12 SGEE1968 pKa = 4.29 WTCTAQDD1975 pKa = 3.52 GTVAAGEE1982 pKa = 4.12 WTVTGRR1988 pKa = 11.84 GDD1990 pKa = 3.42 ATLTGADD1997 pKa = 3.53 GSNPARR2003 pKa = 11.84 ILADD2007 pKa = 3.39 SSCTVAEE2014 pKa = 4.33 SGRR2017 pKa = 11.84 PGNPVHH2023 pKa = 7.25 SDD2025 pKa = 3.09 ASYY2028 pKa = 8.38 TWTTPVEE2035 pKa = 4.39 EE2036 pKa = 4.48 VPVDD2040 pKa = 3.49 YY2041 pKa = 10.66 TNNGVIPAGDD2051 pKa = 3.59 HH2052 pKa = 5.53 VRR2054 pKa = 11.84 ATVTNEE2060 pKa = 3.41 TARR2063 pKa = 11.84 NIGSFQIQKK2072 pKa = 10.87 APIEE2076 pKa = 4.36 GASDD2080 pKa = 3.67 GVTDD2084 pKa = 3.32 TTFAVDD2090 pKa = 3.47 YY2091 pKa = 10.26 SCRR2094 pKa = 11.84 AGTNDD2099 pKa = 4.71 PISGTVEE2106 pKa = 4.06 VQAGGAPVTVAGIPLGSYY2124 pKa = 8.29 CTVSEE2129 pKa = 4.05 QGPQGGLAEE2138 pKa = 4.42 GMTWQHH2144 pKa = 6.64 PEE2146 pKa = 3.82 TYY2148 pKa = 8.63 TVTGATIMIAEE2159 pKa = 4.5 GEE2161 pKa = 4.28 EE2162 pKa = 4.07 QVEE2165 pKa = 4.58 SVTADD2170 pKa = 3.08 EE2171 pKa = 4.58 VQFRR2175 pKa = 11.84 LPVDD2179 pKa = 4.05 PDD2181 pKa = 3.37 AEE2183 pKa = 4.27 AATVTVTNTVLPEE2196 pKa = 3.68 ALIAKK2201 pKa = 7.17 TFTGSAQHH2209 pKa = 6.07 MVDD2212 pKa = 4.06 EE2213 pKa = 5.04 AWDD2216 pKa = 4.07 GTWDD2220 pKa = 3.42 LTYY2223 pKa = 10.49 TVTVTNPSSVQALTYY2238 pKa = 10.63 DD2239 pKa = 3.63 LSDD2242 pKa = 3.62 VLTLPDD2248 pKa = 4.02 SVVLNQVTISGGALTEE2264 pKa = 4.07 TLAADD2269 pKa = 4.27 AMPDD2273 pKa = 3.61 PVITGAEE2280 pKa = 4.05 LPAAADD2286 pKa = 3.79 GEE2288 pKa = 4.76 GTHH2291 pKa = 7.03 TYY2293 pKa = 10.18 TITLNVTGPADD2304 pKa = 3.64 GVNTSGQAEE2313 pKa = 4.76 CSTDD2317 pKa = 3.19 SATSGEE2323 pKa = 4.51 TIHH2326 pKa = 6.8 NSATVTSNGTEE2337 pKa = 4.3 HH2338 pKa = 7.38 SDD2340 pKa = 3.67 EE2341 pKa = 4.74 DD2342 pKa = 4.15 CGSIPEE2348 pKa = 4.26 TPEE2351 pKa = 3.48 VGVEE2355 pKa = 4.18 KK2356 pKa = 9.65 TASTATDD2363 pKa = 3.39 NGDD2366 pKa = 3.74 GTWSIDD2372 pKa = 3.32 YY2373 pKa = 8.47 TITVTNSSEE2382 pKa = 3.9 AVGRR2386 pKa = 11.84 YY2387 pKa = 6.64 TLSDD2391 pKa = 3.45 TPSFGDD2397 pKa = 3.54 GLTVLSAQVRR2407 pKa = 11.84 QPGDD2411 pKa = 4.18 DD2412 pKa = 3.42 EE2413 pKa = 5.17 AQDD2416 pKa = 4.49 LEE2418 pKa = 4.85 GTGPWTLATDD2428 pKa = 3.94 RR2429 pKa = 11.84 TITADD2434 pKa = 3.33 GTHH2437 pKa = 6.91 EE2438 pKa = 3.94 YY2439 pKa = 10.4 HH2440 pKa = 7.5 VIVLAQADD2448 pKa = 4.22 LGANGIPDD2456 pKa = 3.86 TAVCEE2461 pKa = 4.35 PDD2463 pKa = 4.38 GSDD2466 pKa = 3.61 GPGQGFYY2473 pKa = 10.94 NAATVSSAGKK2483 pKa = 8.75 TSDD2486 pKa = 4.02 ADD2488 pKa = 3.5 ACASPAVPEE2497 pKa = 4.22 IVKK2500 pKa = 10.81 SLDD2503 pKa = 3.39 SSDD2506 pKa = 3.5 QRR2508 pKa = 11.84 LADD2511 pKa = 4.16 GAWDD2515 pKa = 3.97 GTWDD2519 pKa = 3.85 LEE2521 pKa = 4.27 YY2522 pKa = 10.74 SITVSNPSADD2532 pKa = 2.99 IAVVYY2537 pKa = 8.94 TLVDD2541 pKa = 3.91 TPALGDD2547 pKa = 3.63 GVTINSGTVTGGQTPDD2563 pKa = 3.11 GFTWTAANGQTQVIVDD2579 pKa = 4.34 APVTLQPGASDD2590 pKa = 4.07 TYY2592 pKa = 8.7 TVRR2595 pKa = 11.84 LNVTVPDD2602 pKa = 3.92 AGVPADD2608 pKa = 5.26 GFDD2611 pKa = 3.71 AQCVGGDD2618 pKa = 3.51 TAAGNQALHH2627 pKa = 5.6 NTATVSAGGTTLEE2640 pKa = 5.01 DD2641 pKa = 3.72 EE2642 pKa = 4.69 ACGSIEE2648 pKa = 4.07 QAAQATIEE2656 pKa = 4.09 KK2657 pKa = 9.24 RR2658 pKa = 11.84 LEE2660 pKa = 4.27 GVPVQQPDD2668 pKa = 3.94 GSWTITYY2675 pKa = 10.66 SLIVSNSSALQARR2688 pKa = 11.84 FDD2690 pKa = 4.84 LSDD2693 pKa = 3.5 EE2694 pKa = 4.27 LNYY2697 pKa = 10.89 GAGITVDD2704 pKa = 3.2 SATIEE2709 pKa = 4.19 LTEE2712 pKa = 3.97 GHH2714 pKa = 5.75 QAVTEE2719 pKa = 4.32 TNWDD2723 pKa = 3.57 GVQNTAVVVGGSIPAAEE2740 pKa = 4.61 VPATDD2745 pKa = 5.27 DD2746 pKa = 5.08 DD2747 pKa = 4.83 PGTPARR2753 pKa = 11.84 IVYY2756 pKa = 8.02 TVTVHH2761 pKa = 7.33 ASAPLATVAPSAADD2775 pKa = 3.47 CTVDD2779 pKa = 4.05 DD2780 pKa = 5.33 GEE2782 pKa = 4.88 DD2783 pKa = 3.3 GRR2785 pKa = 11.84 TGFRR2789 pKa = 11.84 NVASLVSGGQSTTAEE2804 pKa = 4.1 ACGAPAAPTVDD2815 pKa = 3.24 KK2816 pKa = 10.61 TFTSAQRR2823 pKa = 11.84 RR2824 pKa = 11.84 LADD2827 pKa = 4.07 GAWDD2831 pKa = 3.97 GTWDD2835 pKa = 3.85 LEE2837 pKa = 4.27 YY2838 pKa = 10.74 SITVSNPSADD2848 pKa = 2.99 IAVVYY2853 pKa = 8.94 TLVDD2857 pKa = 3.87 TPALGEE2863 pKa = 4.18 GVTINSGTVTGGQTPDD2879 pKa = 3.11 GFTWTAANGQTQVIVDD2895 pKa = 4.34 APVTLQPGASDD2906 pKa = 4.11 TYY2908 pKa = 10.83 TVTLNVNAPASGVNTDD2924 pKa = 3.13 SAIDD2928 pKa = 3.75 ARR2930 pKa = 11.84 CQGSDD2935 pKa = 3.05 TPAGNEE2941 pKa = 3.76 ALHH2944 pKa = 5.89 NQVTVTSGTSEE2955 pKa = 4.13 FTADD2959 pKa = 3.23 ACGDD2963 pKa = 3.67 IPALPAVIVDD2973 pKa = 3.67 KK2974 pKa = 10.6 TVTGTPTLVDD2984 pKa = 3.19 GVMTVKK2990 pKa = 10.85 YY2991 pKa = 9.84 QVTVTNTDD2999 pKa = 2.93 EE3000 pKa = 4.79 ANATRR3005 pKa = 11.84 YY3006 pKa = 7.74 TLTDD3010 pKa = 3.27 TPAFGEE3016 pKa = 4.19 GMEE3019 pKa = 4.7 IISAEE3024 pKa = 4.13 VTGNGAADD3032 pKa = 3.85 DD3033 pKa = 4.2 WDD3035 pKa = 3.96 GTAQPVVVSDD3045 pKa = 3.87 AALEE3049 pKa = 4.4 AGASEE3054 pKa = 4.51 TFTVVIRR3061 pKa = 11.84 AKK3063 pKa = 10.22 IPSSLSSTAGNCTLEE3078 pKa = 4.39 SGEE3081 pKa = 4.59 SGTGALNRR3089 pKa = 11.84 ATVTAGGEE3097 pKa = 4.15 EE3098 pKa = 4.25 MTDD3101 pKa = 3.61 EE3102 pKa = 4.64 ACAEE3106 pKa = 4.28 PPSPTPTPSTSPSPKK3121 pKa = 9.16 PSTPSPHH3128 pKa = 7.0 KK3129 pKa = 10.47 MPFTGADD3136 pKa = 2.47 IRR3138 pKa = 11.84 YY3139 pKa = 8.94 AVWALILIGVGGLAVAGARR3158 pKa = 11.84 RR3159 pKa = 11.84 RR3160 pKa = 11.84 RR3161 pKa = 11.84 KK3162 pKa = 8.3 HH3163 pKa = 5.07 TEE3165 pKa = 3.21 AA3166 pKa = 5.32
Molecular weight: 327.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.757
IPC_protein 3.795
Toseland 3.579
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.617
EMBOSS 3.719
Sillero 3.923
Patrickios 0.985
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A3S0CF16|A0A3S0CF16_9ACTO ATP-dependent Clp protease ATP-binding subunit OS=Actinobaculum sp. 352 OX=2490946 GN=EKN07_09505 PE=3 SV=1
MM1 pKa = 7.42 RR2 pKa = 11.84 QGARR6 pKa = 11.84 ANKK9 pKa = 8.05 HH10 pKa = 3.51 TARR13 pKa = 11.84 LKK15 pKa = 9.88 RR16 pKa = 11.84 AARR19 pKa = 11.84 LKK21 pKa = 10.37 RR22 pKa = 11.84 AAPLKK27 pKa = 10.32 RR28 pKa = 11.84 AVPPKK33 pKa = 10.38 RR34 pKa = 11.84 MAPPRR39 pKa = 11.84 CAALPRR45 pKa = 11.84 RR46 pKa = 11.84 AAPPKK51 pKa = 10.23 RR52 pKa = 11.84 RR53 pKa = 11.84 TRR55 pKa = 11.84 PLARR59 pKa = 11.84 VRR61 pKa = 11.84 GNNMMM66 pKa = 4.75
Molecular weight: 7.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.096
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.501
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.223
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.131
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2387
0
2387
788902
32
3166
330.5
35.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.19 ± 0.061
0.869 ± 0.015
6.098 ± 0.047
6.138 ± 0.055
2.896 ± 0.035
8.64 ± 0.051
2.026 ± 0.022
4.788 ± 0.038
2.37 ± 0.037
9.433 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.111 ± 0.02
2.53 ± 0.028
5.107 ± 0.035
3.311 ± 0.031
6.962 ± 0.07
6.143 ± 0.041
6.387 ± 0.064
8.221 ± 0.047
1.43 ± 0.02
2.351 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here