Amino acid dipepetide frequency for Actinobaculum sp. 352

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.96AlaAla: 16.96 ± 0.212	1.188AlaCys: 1.188 ± 0.039	7.824AlaAsp: 7.824 ± 0.116	7.495AlaGlu: 7.495 ± 0.112	3.245AlaPhe: 3.245 ± 0.07	10.602AlaGly: 10.602 ± 0.138	2.25AlaHis: 2.25 ± 0.057	5.418AlaIle: 5.418 ± 0.097	2.8AlaLys: 2.8 ± 0.066	11.799AlaLeu: 11.799 ± 0.144	2.733AlaMet: 2.733 ± 0.067	2.723AlaAsn: 2.723 ± 0.067	5.045AlaPro: 5.045 ± 0.101	4.642AlaGln: 4.642 ± 0.085	8.457AlaArg: 8.457 ± 0.125	7.176AlaSer: 7.176 ± 0.113	7.219AlaThr: 7.219 ± 0.11	10.094AlaVal: 10.094 ± 0.136	1.669AlaTrp: 1.669 ± 0.051	2.558AlaTyr: 2.558 ± 0.059	0.0AlaXaa: 0.0 ± 0.0
Cys	1.119CysAla: 1.119 ± 0.039	0.127CysCys: 0.127 ± 0.012	0.498CysAsp: 0.498 ± 0.027	0.497CysGlu: 0.497 ± 0.025	0.257CysPhe: 0.257 ± 0.015	1.015CysGly: 1.015 ± 0.037	0.186CysHis: 0.186 ± 0.017	0.365CysIle: 0.365 ± 0.022	0.131CysLys: 0.131 ± 0.013	0.767CysLeu: 0.767 ± 0.035	0.145CysMet: 0.145 ± 0.014	0.172CysAsn: 0.172 ± 0.016	0.463CysPro: 0.463 ± 0.027	0.276CysGln: 0.276 ± 0.019	0.532CysArg: 0.532 ± 0.027	0.549CysSer: 0.549 ± 0.03	0.558CysThr: 0.558 ± 0.04	0.693CysVal: 0.693 ± 0.029	0.122CysTrp: 0.122 ± 0.012	0.214CysTyr: 0.214 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	7.458AspAla: 7.458 ± 0.113	0.406AspCys: 0.406 ± 0.024	4.159AspAsp: 4.159 ± 0.089	4.213AspGlu: 4.213 ± 0.072	1.801AspPhe: 1.801 ± 0.055	6.025AspGly: 6.025 ± 0.127	1.161AspHis: 1.161 ± 0.04	3.013AspIle: 3.013 ± 0.066	1.261AspLys: 1.261 ± 0.046	5.427AspLeu: 5.427 ± 0.088	1.334AspMet: 1.334 ± 0.043	1.361AspAsn: 1.361 ± 0.05	3.733AspPro: 3.733 ± 0.071	1.616AspGln: 1.616 ± 0.047	3.493AspArg: 3.493 ± 0.075	3.449AspSer: 3.449 ± 0.071	3.308AspThr: 3.308 ± 0.077	5.164AspVal: 5.164 ± 0.086	0.935AspTrp: 0.935 ± 0.04	1.658AspTyr: 1.658 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	7.218GluAla: 7.218 ± 0.133	0.461GluCys: 0.461 ± 0.021	3.382GluAsp: 3.382 ± 0.064	4.462GluGlu: 4.462 ± 0.087	1.648GluPhe: 1.648 ± 0.04	4.347GluGly: 4.347 ± 0.078	1.434GluHis: 1.434 ± 0.046	3.121GluIle: 3.121 ± 0.071	1.853GluLys: 1.853 ± 0.051	6.621GluLeu: 6.621 ± 0.113	1.368GluMet: 1.368 ± 0.042	1.74GluAsn: 1.74 ± 0.046	2.81GluPro: 2.81 ± 0.072	2.607GluGln: 2.607 ± 0.06	4.741GluArg: 4.741 ± 0.096	3.272GluSer: 3.272 ± 0.07	3.273GluThr: 3.273 ± 0.07	4.618GluVal: 4.618 ± 0.087	0.829GluTrp: 0.829 ± 0.038	1.578GluTyr: 1.578 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	3.402PheAla: 3.402 ± 0.064	0.293PheCys: 0.293 ± 0.015	2.108PheAsp: 2.108 ± 0.055	1.544PheGlu: 1.544 ± 0.043	1.077PhePhe: 1.077 ± 0.043	2.836PheGly: 2.836 ± 0.075	0.575PheHis: 0.575 ± 0.029	1.347PheIle: 1.347 ± 0.04	0.605PheLys: 0.605 ± 0.031	2.609PheLeu: 2.609 ± 0.068	0.527PheMet: 0.527 ± 0.028	0.805PheAsn: 0.805 ± 0.034	1.401PhePro: 1.401 ± 0.043	0.785PheGln: 0.785 ± 0.03	1.492PheArg: 1.492 ± 0.041	2.06PheSer: 2.06 ± 0.055	2.092PheThr: 2.092 ± 0.059	2.348PheVal: 2.348 ± 0.056	0.38PheTrp: 0.38 ± 0.024	0.678PheTyr: 0.678 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	9.257GlyAla: 9.257 ± 0.139	0.802GlyCys: 0.802 ± 0.034	4.85GlyAsp: 4.85 ± 0.09	5.312GlyGlu: 5.312 ± 0.083	2.784GlyPhe: 2.784 ± 0.058	7.205GlyGly: 7.205 ± 0.127	1.822GlyHis: 1.822 ± 0.05	4.603GlyIle: 4.603 ± 0.074	2.687GlyLys: 2.687 ± 0.062	7.682GlyLeu: 7.682 ± 0.128	2.317GlyMet: 2.317 ± 0.062	2.299GlyAsn: 2.299 ± 0.06	3.392GlyPro: 3.392 ± 0.068	2.999GlyGln: 2.999 ± 0.064	5.819GlyArg: 5.819 ± 0.086	5.508GlySer: 5.508 ± 0.095	5.96GlyThr: 5.96 ± 0.111	7.228GlyVal: 7.228 ± 0.107	1.484GlyTrp: 1.484 ± 0.047	2.388GlyTyr: 2.388 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.265HisAla: 2.265 ± 0.051	0.184HisCys: 0.184 ± 0.014	1.246HisAsp: 1.246 ± 0.045	1.148HisGlu: 1.148 ± 0.041	0.537HisPhe: 0.537 ± 0.027	1.806HisGly: 1.806 ± 0.045	0.525HisHis: 0.525 ± 0.028	0.965HisIle: 0.965 ± 0.039	0.404HisLys: 0.404 ± 0.022	1.894HisLeu: 1.894 ± 0.052	0.45HisMet: 0.45 ± 0.024	0.504HisAsn: 0.504 ± 0.025	1.34HisPro: 1.34 ± 0.043	0.548HisGln: 0.548 ± 0.029	1.539HisArg: 1.539 ± 0.048	1.227HisSer: 1.227 ± 0.038	1.279HisThr: 1.279 ± 0.046	1.612HisVal: 1.612 ± 0.044	0.289HisTrp: 0.289 ± 0.019	0.498HisTyr: 0.498 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.315IleAla: 6.315 ± 0.099	0.485IleCys: 0.485 ± 0.024	3.306IleAsp: 3.306 ± 0.063	2.775IleGlu: 2.775 ± 0.066	1.251IlePhe: 1.251 ± 0.048	4.41IleGly: 4.41 ± 0.08	0.911IleHis: 0.911 ± 0.037	2.322IleIle: 2.322 ± 0.066	0.952IleLys: 0.952 ± 0.043	4.153IleLeu: 4.153 ± 0.08	0.918IleMet: 0.918 ± 0.037	1.269IleAsn: 1.269 ± 0.047	2.689IlePro: 2.689 ± 0.056	1.136IleGln: 1.136 ± 0.033	3.049IleArg: 3.049 ± 0.068	3.004IleSer: 3.004 ± 0.06	3.36IleThr: 3.36 ± 0.065	4.074IleVal: 4.074 ± 0.074	0.525IleTrp: 0.525 ± 0.029	0.972IleTyr: 0.972 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	2.848LysAla: 2.848 ± 0.071	0.125LysCys: 0.125 ± 0.014	1.368LysAsp: 1.368 ± 0.051	1.453LysGlu: 1.453 ± 0.044	0.503LysPhe: 0.503 ± 0.024	1.669LysGly: 1.669 ± 0.053	0.421LysHis: 0.421 ± 0.021	1.115LysIle: 1.115 ± 0.039	0.975LysLys: 0.975 ± 0.043	2.103LysLeu: 2.103 ± 0.051	0.567LysMet: 0.567 ± 0.028	0.787LysAsn: 0.787 ± 0.031	1.293LysPro: 1.293 ± 0.044	0.894LysGln: 0.894 ± 0.035	1.827LysArg: 1.827 ± 0.054	1.298LysSer: 1.298 ± 0.04	1.517LysThr: 1.517 ± 0.05	2.033LysVal: 2.033 ± 0.057	0.308LysTrp: 0.308 ± 0.021	0.593LysTyr: 0.593 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	12.729LeuAla: 12.729 ± 0.145	0.856LeuCys: 0.856 ± 0.03	5.866LeuAsp: 5.866 ± 0.107	5.286LeuGlu: 5.286 ± 0.087	2.578LeuPhe: 2.578 ± 0.068	8.125LeuGly: 8.125 ± 0.124	1.92LeuHis: 1.92 ± 0.055	4.317LeuIle: 4.317 ± 0.093	1.887LeuLys: 1.887 ± 0.055	9.403LeuLeu: 9.403 ± 0.152	1.728LeuMet: 1.728 ± 0.054	2.226LeuAsn: 2.226 ± 0.053	5.266LeuPro: 5.266 ± 0.081	2.615LeuGln: 2.615 ± 0.06	6.945LeuArg: 6.945 ± 0.11	5.835LeuSer: 5.835 ± 0.088	6.077LeuThr: 6.077 ± 0.096	7.689LeuVal: 7.689 ± 0.131	1.133LeuTrp: 1.133 ± 0.038	1.851LeuTyr: 1.851 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	2.545MetAla: 2.545 ± 0.062	0.199MetCys: 0.199 ± 0.017	1.118MetAsp: 1.118 ± 0.041	1.013MetGlu: 1.013 ± 0.037	0.608MetPhe: 0.608 ± 0.03	1.55MetGly: 1.55 ± 0.045	0.378MetHis: 0.378 ± 0.021	1.014MetIle: 1.014 ± 0.037	0.532MetLys: 0.532 ± 0.026	2.009MetLeu: 2.009 ± 0.053	0.401MetMet: 0.401 ± 0.023	0.621MetAsn: 0.621 ± 0.025	1.265MetPro: 1.265 ± 0.045	0.69MetGln: 0.69 ± 0.03	1.714MetArg: 1.714 ± 0.045	1.574MetSer: 1.574 ± 0.054	1.682MetThr: 1.682 ± 0.044	1.555MetVal: 1.555 ± 0.045	0.252MetTrp: 0.252 ± 0.02	0.393MetTyr: 0.393 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	3.007AsnAla: 3.007 ± 0.061	0.186AsnCys: 0.186 ± 0.016	1.422AsnAsp: 1.422 ± 0.047	1.435AsnGlu: 1.435 ± 0.045	0.757AsnPhe: 0.757 ± 0.033	2.391AsnGly: 2.391 ± 0.058	0.466AsnHis: 0.466 ± 0.026	1.205AsnIle: 1.205 ± 0.036	0.562AsnLys: 0.562 ± 0.03	2.221AsnLeu: 2.221 ± 0.062	0.558AsnMet: 0.558 ± 0.026	0.78AsnAsn: 0.78 ± 0.04	1.775AsnPro: 1.775 ± 0.051	0.758AsnGln: 0.758 ± 0.03	1.666AsnArg: 1.666 ± 0.04	1.506AsnSer: 1.506 ± 0.045	1.583AsnThr: 1.583 ± 0.051	2.004AsnVal: 2.004 ± 0.057	0.382AsnTrp: 0.382 ± 0.021	0.634AsnTyr: 0.634 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	5.768ProAla: 5.768 ± 0.123	0.326ProCys: 0.326 ± 0.022	3.571ProAsp: 3.571 ± 0.062	4.016ProGlu: 4.016 ± 0.072	1.426ProPhe: 1.426 ± 0.04	4.718ProGly: 4.718 ± 0.077	1.151ProHis: 1.151 ± 0.041	2.092ProIle: 2.092 ± 0.048	1.05ProLys: 1.05 ± 0.046	4.421ProLeu: 4.421 ± 0.07	0.891ProMet: 0.891 ± 0.033	1.282ProAsn: 1.282 ± 0.047	2.128ProPro: 2.128 ± 0.072	1.943ProGln: 1.943 ± 0.053	3.083ProArg: 3.083 ± 0.065	3.336ProSer: 3.336 ± 0.077	3.535ProThr: 3.535 ± 0.076	4.36ProVal: 4.36 ± 0.085	0.724ProTrp: 0.724 ± 0.033	1.252ProTyr: 1.252 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	4.419GlnAla: 4.419 ± 0.08	0.271GlnCys: 0.271 ± 0.018	1.52GlnAsp: 1.52 ± 0.045	2.069GlnGlu: 2.069 ± 0.058	0.794GlnPhe: 0.794 ± 0.031	2.32GlnGly: 2.32 ± 0.055	0.748GlnHis: 0.748 ± 0.03	1.744GlnIle: 1.744 ± 0.044	0.81GlnLys: 0.81 ± 0.034	3.308GlnLeu: 3.308 ± 0.077	0.802GlnMet: 0.802 ± 0.033	0.788GlnAsn: 0.788 ± 0.032	1.607GlnPro: 1.607 ± 0.052	1.493GlnGln: 1.493 ± 0.049	2.886GlnArg: 2.886 ± 0.068	1.763GlnSer: 1.763 ± 0.058	1.828GlnThr: 1.828 ± 0.044	2.651GlnVal: 2.651 ± 0.061	0.553GlnTrp: 0.553 ± 0.026	0.731GlnTyr: 0.731 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	7.49ArgAla: 7.49 ± 0.101	0.578ArgCys: 0.578 ± 0.027	3.947ArgAsp: 3.947 ± 0.08	4.873ArgGlu: 4.873 ± 0.099	2.06ArgPhe: 2.06 ± 0.058	5.125ArgGly: 5.125 ± 0.085	1.571ArgHis: 1.571 ± 0.044	3.725ArgIle: 3.725 ± 0.074	1.785ArgLys: 1.785 ± 0.053	6.75ArgLeu: 6.75 ± 0.116	1.663ArgMet: 1.663 ± 0.046	1.738ArgAsn: 1.738 ± 0.044	3.194ArgPro: 3.194 ± 0.071	2.554ArgGln: 2.554 ± 0.071	6.252ArgArg: 6.252 ± 0.114	4.193ArgSer: 4.193 ± 0.078	3.988ArgThr: 3.988 ± 0.076	5.177ArgVal: 5.177 ± 0.082	1.136ArgTrp: 1.136 ± 0.039	1.82ArgTyr: 1.82 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	7.271SerAla: 7.271 ± 0.11	0.497SerCys: 0.497 ± 0.03	3.705SerAsp: 3.705 ± 0.085	3.396SerGlu: 3.396 ± 0.077	2.05SerPhe: 2.05 ± 0.062	6.349SerGly: 6.349 ± 0.095	1.214SerHis: 1.214 ± 0.042	2.647SerIle: 2.647 ± 0.054	1.301SerLys: 1.301 ± 0.042	5.537SerLeu: 5.537 ± 0.083	1.284SerMet: 1.284 ± 0.037	1.543SerAsn: 1.543 ± 0.045	3.191SerPro: 3.191 ± 0.079	1.99SerGln: 1.99 ± 0.044	4.003SerArg: 4.003 ± 0.08	4.4SerSer: 4.4 ± 0.099	4.002SerThr: 4.002 ± 0.086	4.591SerVal: 4.591 ± 0.082	0.949SerTrp: 0.949 ± 0.033	1.515SerTyr: 1.515 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	7.825ThrAla: 7.825 ± 0.129	0.546ThrCys: 0.546 ± 0.037	3.573ThrAsp: 3.573 ± 0.069	3.332ThrGlu: 3.332 ± 0.07	1.873ThrPhe: 1.873 ± 0.05	5.989ThrGly: 5.989 ± 0.104	1.252ThrHis: 1.252 ± 0.04	3.184ThrIle: 3.184 ± 0.074	1.334ThrLys: 1.334 ± 0.047	5.726ThrLeu: 5.726 ± 0.085	1.107ThrMet: 1.107 ± 0.04	1.573ThrAsn: 1.573 ± 0.052	4.096ThrPro: 4.096 ± 0.089	1.815ThrGln: 1.815 ± 0.054	3.903ThrArg: 3.903 ± 0.075	4.02ThrSer: 4.02 ± 0.087	4.481ThrThr: 4.481 ± 0.113	5.818ThrVal: 5.818 ± 0.152	0.953ThrTrp: 0.953 ± 0.037	1.473ThrTyr: 1.473 ± 0.052	0.0ThrXaa: 0.0 ± 0.0
Val	10.082ValAla: 10.082 ± 0.126	0.8ValCys: 0.8 ± 0.034	5.2ValAsp: 5.2 ± 0.088	4.764ValGlu: 4.764 ± 0.091	2.452ValPhe: 2.452 ± 0.058	6.702ValGly: 6.702 ± 0.108	1.503ValHis: 1.503 ± 0.046	4.068ValIle: 4.068 ± 0.074	1.86ValLys: 1.86 ± 0.051	8.049ValLeu: 8.049 ± 0.12	1.524ValMet: 1.524 ± 0.046	2.005ValAsn: 2.005 ± 0.057	4.49ValPro: 4.49 ± 0.083	2.311ValGln: 2.311 ± 0.056	5.419ValArg: 5.419 ± 0.092	5.01ValSer: 5.01 ± 0.079	5.639ValThr: 5.639 ± 0.127	7.608ValVal: 7.608 ± 0.123	1.015ValTrp: 1.015 ± 0.038	1.711ValTyr: 1.711 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	1.43TrpAla: 1.43 ± 0.051	0.147TrpCys: 0.147 ± 0.013	0.89TrpAsp: 0.89 ± 0.036	0.829TrpGlu: 0.829 ± 0.036	0.48TrpPhe: 0.48 ± 0.029	0.979TrpGly: 0.979 ± 0.038	0.309TrpHis: 0.309 ± 0.021	0.693TrpIle: 0.693 ± 0.033	0.398TrpLys: 0.398 ± 0.023	1.494TrpLeu: 1.494 ± 0.05	0.354TrpMet: 0.354 ± 0.02	0.473TrpAsn: 0.473 ± 0.022	0.678TrpPro: 0.678 ± 0.031	0.622TrpGln: 0.622 ± 0.03	1.079TrpArg: 1.079 ± 0.044	0.843TrpSer: 0.843 ± 0.033	0.937TrpThr: 0.937 ± 0.038	1.047TrpVal: 1.047 ± 0.034	0.3TrpTrp: 0.3 ± 0.019	0.316TrpTyr: 0.316 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.487TyrAla: 2.487 ± 0.065	0.209TyrCys: 0.209 ± 0.017	1.425TyrAsp: 1.425 ± 0.05	1.47TyrGlu: 1.47 ± 0.048	0.781TyrPhe: 0.781 ± 0.035	2.236TyrGly: 2.236 ± 0.055	0.463TyrHis: 0.463 ± 0.024	0.916TyrIle: 0.916 ± 0.035	0.512TyrLys: 0.512 ± 0.024	2.362TyrLeu: 2.362 ± 0.06	0.445TyrMet: 0.445 ± 0.024	0.606TyrAsn: 0.606 ± 0.031	1.218TyrPro: 1.218 ± 0.043	0.813TyrGln: 0.813 ± 0.032	1.72TyrArg: 1.72 ± 0.045	1.412TyrSer: 1.412 ± 0.048	1.555TyrThr: 1.555 ± 0.054	1.846TyrVal: 1.846 ± 0.049	0.359TyrTrp: 0.359 ± 0.019	0.673TyrTyr: 0.673 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2387 proteins (788902 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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