Prauserella rugosa

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Prauserella

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4886 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A660CIE3|A0A660CIE3_9PSEU Uncharacterized protein OS=Prauserella rugosa OX=43354 GN=JD82_03473 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84SDD4 pKa = 3.02KK5 pKa = 10.89RR6 pKa = 11.84RR7 pKa = 11.84IGVLAGAGTAAVAVTLSMAGTANAVPQEE35 pKa = 4.11GTVLGAGSATAVEE48 pKa = 4.38GKK50 pKa = 10.65YY51 pKa = 10.23IVVLKK56 pKa = 10.97DD57 pKa = 3.51GVQTTAADD65 pKa = 3.44LGGEE69 pKa = 4.3VNHH72 pKa = 6.6TFSTVLNGYY81 pKa = 8.7SADD84 pKa = 3.52MSEE87 pKa = 4.22ALAEE91 pKa = 3.89RR92 pKa = 11.84VAANPAVDD100 pKa = 3.72YY101 pKa = 11.11VEE103 pKa = 4.59QVQTVHH109 pKa = 6.0TMADD113 pKa = 3.36QTDD116 pKa = 3.97PPSWGLDD123 pKa = 3.18RR124 pKa = 11.84IDD126 pKa = 4.0QAEE129 pKa = 4.32LPLDD133 pKa = 3.61GTYY136 pKa = 9.76TYY138 pKa = 10.17PNEE141 pKa = 4.55GEE143 pKa = 4.47GVTAYY148 pKa = 9.87IIDD151 pKa = 3.76TGIMTSHH158 pKa = 6.44QDD160 pKa = 3.08FGGRR164 pKa = 11.84ATAGFDD170 pKa = 3.57AVDD173 pKa = 3.57SGGDD177 pKa = 3.41AEE179 pKa = 4.85DD180 pKa = 4.54CNGHH184 pKa = 5.09GTHH187 pKa = 6.37VAGTVGGSEE196 pKa = 4.11YY197 pKa = 11.07GVAKK201 pKa = 10.5SADD204 pKa = 3.53LVAVRR209 pKa = 11.84VLNCQGSGTNEE220 pKa = 3.58QVIAGIDD227 pKa = 3.39WVTEE231 pKa = 4.04NASGPSVANMSLGGSASAAIDD252 pKa = 3.58DD253 pKa = 4.28AVQNSIASGVTYY265 pKa = 10.84GLAAGNDD272 pKa = 3.88SGADD276 pKa = 3.39ACNSSPARR284 pKa = 11.84VPEE287 pKa = 4.12GLTVGSTTEE296 pKa = 3.46TDD298 pKa = 3.2ARR300 pKa = 11.84SSFSNIGSCVDD311 pKa = 3.37IFAPGSDD318 pKa = 3.5ITSAWIDD325 pKa = 3.28SDD327 pKa = 3.54TSEE330 pKa = 4.08NTISGTSMATPHH342 pKa = 5.72VVGAAALYY350 pKa = 10.02LAANPDD356 pKa = 3.45ATPAQVGDD364 pKa = 3.8ALVAGGSEE372 pKa = 4.06GVVGNPGSGSPNVLLNVVTDD392 pKa = 4.86GSAPDD397 pKa = 4.65PDD399 pKa = 5.09PEE401 pKa = 4.6PEE403 pKa = 4.78PDD405 pKa = 3.89PGTCEE410 pKa = 4.16AVTNDD415 pKa = 4.09EE416 pKa = 4.04VTAIPDD422 pKa = 3.67AGSVDD427 pKa = 3.64STVTVSGCDD436 pKa = 3.18AASAEE441 pKa = 4.26ATVEE445 pKa = 3.62VDD447 pKa = 2.5ITHH450 pKa = 7.53TYY452 pKa = 10.14RR453 pKa = 11.84GDD455 pKa = 3.28IALDD459 pKa = 4.35LIAPGGAEE467 pKa = 4.0FSLKK471 pKa = 9.82QASFDD476 pKa = 3.57SGTDD480 pKa = 2.79ITEE483 pKa = 4.25TYY485 pKa = 9.78TVDD488 pKa = 3.21LSGVTEE494 pKa = 4.63VDD496 pKa = 3.77GEE498 pKa = 4.34WTLKK502 pKa = 9.15VTDD505 pKa = 3.84SFVYY509 pKa = 9.08DD510 pKa = 3.54TGTLNSWTLSLL521 pKa = 4.75

Molecular weight: 52.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A660CIX2|A0A660CIX2_9PSEU Glycerol-3-phosphate dehydrogenase OS=Prauserella rugosa OX=43354 GN=JD82_02776 PE=3 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84THH19 pKa = 5.69GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVAARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.62GRR42 pKa = 11.84QQLSAA47 pKa = 4.05

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4886

0

4886

1566181

39

3507

320.5

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.199 ± 0.051

0.728 ± 0.008

6.549 ± 0.03

6.042 ± 0.03

2.721 ± 0.022

9.593 ± 0.036

2.235 ± 0.016

3.367 ± 0.024

1.978 ± 0.027

9.794 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.818 ± 0.013

1.839 ± 0.015

5.799 ± 0.03

2.759 ± 0.021

7.809 ± 0.037

5.242 ± 0.023

6.12 ± 0.028

9.026 ± 0.035

1.414 ± 0.017

1.968 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski