Proteome-pI 2.0 - Proteome Isoelectric Point Database

Lactococcus hodotermopsidis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Lactococcus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 2078 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A6A0BDX9|A0A6A0BDX9_9LACT Uncharacterized protein OS=Lactococcus hodotermopsidis OX=2709157 GN=Hs30E_05880 PE=4 SV=1

MM1 pKa = 7.58SEE3 pKa = 3.96HH4 pKa = 4.82THH6 pKa = 4.76EE7 pKa = 5.15HH8 pKa = 4.7NHH10 pKa = 3.96EE11 pKa = 4.37HH12 pKa = 5.58EE13 pKa = 4.83AEE15 pKa = 4.7FITLVDD21 pKa = 3.81DD22 pKa = 5.43LGNEE26 pKa = 4.18SLFEE30 pKa = 4.02VLLTIDD36 pKa = 3.59GQEE39 pKa = 4.05EE40 pKa = 4.07FGKK43 pKa = 10.51NYY45 pKa = 10.4VLVVPAGAEE54 pKa = 3.67EE55 pKa = 4.49DD56 pKa = 3.77EE57 pKa = 4.49NGEE60 pKa = 4.38VEE62 pKa = 4.23IQAYY66 pKa = 10.08AYY68 pKa = 7.12TQNEE72 pKa = 4.61DD73 pKa = 3.34GTEE76 pKa = 4.05GDD78 pKa = 4.18LMPIEE83 pKa = 4.6EE84 pKa = 4.85DD85 pKa = 5.2SEE87 pKa = 5.01KK88 pKa = 9.5EE89 pKa = 3.58WNMIEE94 pKa = 4.45EE95 pKa = 4.38VFNTFVEE102 pKa = 4.61EE103 pKa = 4.14QNN105 pKa = 3.46

Molecular weight: 12.06 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A6A0BBR0|A0A6A0BBR0_9LACT Pyridoxal phosphate homeostasis protein OS=Lactococcus hodotermopsidis OX=2709157 GN=ytdF PE=3 SV=1

MM1 pKa = 7.92TIRR4 pKa = 11.84KK5 pKa = 7.06LTKK8 pKa = 10.13LALLTALSCVLRR20 pKa = 11.84LAFGSLPNIKK30 pKa = 9.95PITALFFVIMLTFGLVDD47 pKa = 3.68GLFVSSLTMLVTGFLMGFSPIIIGQILSYY76 pKa = 11.05AVILTIGFGLFKK88 pKa = 10.54GVRR91 pKa = 11.84NLIYY95 pKa = 8.85RR96 pKa = 11.84TIIIFCLTMLYY107 pKa = 10.8GFVISIFSARR117 pKa = 11.84LFGASFLPFWLSGLTFDD134 pKa = 5.46LAHH137 pKa = 6.65AVSTALFFPVLVTIFKK153 pKa = 10.48NKK155 pKa = 8.36QVYY158 pKa = 9.3IKK160 pKa = 10.59RR161 pKa = 3.76

Molecular weight: 17.86 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
2078	0	2078	642429	39	4467	309.2	34.58

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
7.981 ± 0.058	0.51 ± 0.013	5.982 ± 0.07	6.743 ± 0.061	4.841 ± 0.054	6.305 ± 0.058	1.786 ± 0.024	7.699 ± 0.049	7.134 ± 0.056	9.535 ± 0.071

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.497 ± 0.028	4.97 ± 0.042	3.188 ± 0.031	3.544 ± 0.034	3.703 ± 0.039	6.001 ± 0.081	6.191 ± 0.057	6.963 ± 0.052	0.854 ± 0.018	3.572 ± 0.04

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski