Drosophila simulans (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae;

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15133 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4R339|B4R339_DROSI GD16585 OS=Drosophila simulans OX=7240 GN=Dsim\GD16585 PE=4 SV=1
MM1 pKa = 8.13SEE3 pKa = 5.87DD4 pKa = 3.96KK5 pKa = 11.0PQDD8 pKa = 3.39YY9 pKa = 10.55KK10 pKa = 11.33KK11 pKa = 10.44RR12 pKa = 11.84LSIEE16 pKa = 4.01LTNSLLRR23 pKa = 11.84EE24 pKa = 4.6DD25 pKa = 3.85NNIWASADD33 pKa = 3.35LGMGPDD39 pKa = 4.01LQDD42 pKa = 3.03MLGPEE47 pKa = 4.01EE48 pKa = 5.04AYY50 pKa = 10.24EE51 pKa = 4.04GLYY54 pKa = 10.37GQGPPSVHH62 pKa = 6.44SSHH65 pKa = 6.82GGRR68 pKa = 11.84AFHH71 pKa = 5.89QQGYY75 pKa = 7.61DD76 pKa = 3.39TLPIDD81 pKa = 4.02SMQGLEE87 pKa = 3.78ISSPVGGGGAGGAPGNGGAVGGASGGGGNIGAIPPSGAPTSPYY130 pKa = 10.96SMDD133 pKa = 3.35MDD135 pKa = 3.9VGEE138 pKa = 4.73IDD140 pKa = 4.47AGALNFDD147 pKa = 4.93LDD149 pKa = 4.88AMPTPPNDD157 pKa = 3.91NNNLAAWYY165 pKa = 7.63DD166 pKa = 3.78TDD168 pKa = 4.08CC169 pKa = 5.57

Molecular weight: 17.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4QAD1|B4QAD1_DROSI GD23772 OS=Drosophila simulans OX=7240 GN=Dsim\GD23772 PE=3 SV=1
MM1 pKa = 7.64KK2 pKa = 10.56LFIALSVLVAVASALPQFGGNANANANANANAQGGFGGLGGRR44 pKa = 11.84PGFGGGPGFGGGPGFGGGPGFGGGPGFGGRR74 pKa = 11.84PGFGGGPGFGGGPGFGGRR92 pKa = 11.84PGFGGGPGFGGGPGFGGRR110 pKa = 11.84PGFGGGSGFGGGSSSASASSSASASGGGRR139 pKa = 11.84GGAGSASASSSANASGGRR157 pKa = 11.84FGG159 pKa = 5.08

Molecular weight: 13.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14772

361

15133

6249901

17

8412

413.0

45.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.374 ± 0.026

1.92 ± 0.016

5.107 ± 0.016

6.283 ± 0.028

3.612 ± 0.014

6.071 ± 0.025

2.647 ± 0.012

4.878 ± 0.016

5.492 ± 0.025

9.184 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.01

4.58 ± 0.017

5.417 ± 0.022

5.064 ± 0.026

5.606 ± 0.019

8.18 ± 0.028

5.53 ± 0.018

5.902 ± 0.015

1.051 ± 0.007

2.987 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski