Lachancea lanzarotensis
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5055 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C7MUT3|A0A0C7MUT3_9SACH AP complex subunit beta OS=Lachancea lanzarotensis OX=1245769 GN=LALA0_S08e05842g PE=3 SV=1
MM1 pKa = 7.74 TYY3 pKa = 10.24 SQQDD7 pKa = 3.43 FQDD10 pKa = 3.62 EE11 pKa = 4.64 VFSFSLTNDD20 pKa = 2.95 APALQHH26 pKa = 6.41 SPSSYY31 pKa = 11.07 GSATDD36 pKa = 4.04 MIPDD40 pKa = 3.65 EE41 pKa = 4.55 EE42 pKa = 4.4 FLIEE46 pKa = 4.26 SPGFGAVAHH55 pKa = 6.18 TADD58 pKa = 5.58 DD59 pKa = 4.33 DD60 pKa = 4.84 DD61 pKa = 5.02 EE62 pKa = 6.03 LFALDD67 pKa = 4.68 TEE69 pKa = 5.15 LSMLPRR75 pKa = 11.84 SYY77 pKa = 11.48 DD78 pKa = 3.52 CLPIDD83 pKa = 4.48 DD84 pKa = 5.12 AKK86 pKa = 10.86 TMVSDD91 pKa = 3.94 YY92 pKa = 11.01 SLPIPDD98 pKa = 3.8 QFANAAQDD106 pKa = 3.99 NYY108 pKa = 10.65 RR109 pKa = 11.84 LWLAGVV115 pKa = 3.81
Molecular weight: 12.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.843
IPC2_protein 3.478
IPC_protein 3.478
Toseland 3.249
ProMoST 3.681
Dawson 3.49
Bjellqvist 3.656
Wikipedia 3.478
Rodwell 3.312
Grimsley 3.172
Solomon 3.465
Lehninger 3.427
Nozaki 3.617
DTASelect 3.884
Thurlkill 3.338
EMBOSS 3.478
Sillero 3.605
Patrickios 0.477
IPC_peptide 3.452
IPC2_peptide 3.567
IPC2.peptide.svr19 3.634
Protein with the highest isoelectric point:
>tr|A0A0C7NAS4|A0A0C7NAS4_9SACH LALA0S15e00716g1_1 OS=Lachancea lanzarotensis OX=1245769 GN=LALA0_S15e00716g PE=3 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.09 WRR6 pKa = 11.84 KK7 pKa = 8.87 KK8 pKa = 8.73 RR9 pKa = 11.84 VRR11 pKa = 11.84 RR12 pKa = 11.84 LKK14 pKa = 9.71 RR15 pKa = 11.84 KK16 pKa = 8.27 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 9.19 VRR21 pKa = 11.84 ARR23 pKa = 11.84 SKK25 pKa = 10.98
Molecular weight: 3.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5055
0
5055
2472229
25
4925
489.1
54.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.72 ± 0.03
1.262 ± 0.013
5.828 ± 0.026
6.563 ± 0.033
4.382 ± 0.02
5.591 ± 0.032
2.26 ± 0.013
5.379 ± 0.022
6.408 ± 0.027
9.878 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.991 ± 0.01
4.835 ± 0.018
4.468 ± 0.024
4.343 ± 0.032
5.038 ± 0.022
8.969 ± 0.05
5.623 ± 0.024
6.325 ± 0.023
1.091 ± 0.01
3.047 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here