Sphingobium sp. EP60837

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4048 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A173KUZ2|A0A173KUZ2_9SPHN Cytochrome-c oxidase OS=Sphingobium sp. EP60837 OX=1855519 GN=coxD PE=4 SV=1
MM1 pKa = 7.68SARR4 pKa = 11.84FWLFSMASAGAALAGGLALGLYY26 pKa = 7.78ATTPPRR32 pKa = 11.84IAFTDD37 pKa = 3.47AEE39 pKa = 4.42IPYY42 pKa = 10.02SAQQEE47 pKa = 4.73SASQPDD53 pKa = 3.86VTGLSGPTDD62 pKa = 3.86IKK64 pKa = 11.19CQGCGPTLADD74 pKa = 3.41RR75 pKa = 11.84QMAAMMGGGWSGYY88 pKa = 10.07DD89 pKa = 3.32DD90 pKa = 4.17PAVRR94 pKa = 11.84DD95 pKa = 3.77YY96 pKa = 10.32MAQEE100 pKa = 4.06YY101 pKa = 10.52SADD104 pKa = 3.84DD105 pKa = 4.47DD106 pKa = 4.72APLPQDD112 pKa = 3.76YY113 pKa = 8.68EE114 pKa = 4.2QPTPVRR120 pKa = 11.84LPANIEE126 pKa = 3.67RR127 pKa = 11.84FAAGEE132 pKa = 4.1SDD134 pKa = 3.26GSQPVQIAQGSVATASPVTATSYY157 pKa = 11.35

Molecular weight: 16.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A173KWL9|A0A173KWL9_9SPHN CbiA domain-containing protein OS=Sphingobium sp. EP60837 OX=1855519 GN=EP837_01702 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4048

0

4048

1252722

30

2884

309.5

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.76 ± 0.049

0.846 ± 0.013

5.845 ± 0.032

5.549 ± 0.037

3.538 ± 0.024

8.778 ± 0.039

2.072 ± 0.021

5.208 ± 0.026

3.198 ± 0.027

9.967 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.557 ± 0.02

2.654 ± 0.024

5.255 ± 0.028

3.345 ± 0.022

7.285 ± 0.04

5.555 ± 0.032

5.007 ± 0.033

6.921 ± 0.034

1.417 ± 0.016

2.243 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski