Amino acid dipepetide frequency for Sphingobium sp. EP60837

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.078AlaAla: 18.078 ± 0.188	1.078AlaCys: 1.078 ± 0.035	7.188AlaAsp: 7.188 ± 0.083	7.478AlaGlu: 7.478 ± 0.094	4.19AlaPhe: 4.19 ± 0.064	10.67AlaGly: 10.67 ± 0.126	2.415AlaHis: 2.415 ± 0.047	6.641AlaIle: 6.641 ± 0.076	4.282AlaLys: 4.282 ± 0.07	13.635AlaLeu: 13.635 ± 0.135	3.793AlaMet: 3.793 ± 0.056	3.111AlaAsn: 3.111 ± 0.055	5.906AlaPro: 5.906 ± 0.076	4.796AlaGln: 4.796 ± 0.06	9.226AlaArg: 9.226 ± 0.101	6.61AlaSer: 6.61 ± 0.083	6.095AlaThr: 6.095 ± 0.069	8.303AlaVal: 8.303 ± 0.098	1.515AlaTrp: 1.515 ± 0.034	2.588AlaTyr: 2.588 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.027CysAla: 1.027 ± 0.039	0.109CysCys: 0.109 ± 0.011	0.543CysAsp: 0.543 ± 0.023	0.436CysGlu: 0.436 ± 0.017	0.296CysPhe: 0.296 ± 0.017	0.892CysGly: 0.892 ± 0.029	0.235CysHis: 0.235 ± 0.018	0.381CysIle: 0.381 ± 0.019	0.197CysLys: 0.197 ± 0.012	0.735CysLeu: 0.735 ± 0.026	0.187CysMet: 0.187 ± 0.011	0.233CysAsn: 0.233 ± 0.014	0.516CysPro: 0.516 ± 0.027	0.224CysGln: 0.224 ± 0.014	0.6CysArg: 0.6 ± 0.024	0.505CysSer: 0.505 ± 0.024	0.417CysThr: 0.417 ± 0.022	0.598CysVal: 0.598 ± 0.026	0.14CysTrp: 0.14 ± 0.008	0.19CysTyr: 0.19 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.239AspAla: 7.239 ± 0.085	0.474AspCys: 0.474 ± 0.022	3.119AspAsp: 3.119 ± 0.06	3.439AspGlu: 3.439 ± 0.055	2.183AspPhe: 2.183 ± 0.045	5.334AspGly: 5.334 ± 0.089	1.346AspHis: 1.346 ± 0.034	3.114AspIle: 3.114 ± 0.053	1.72AspLys: 1.72 ± 0.045	5.89AspLeu: 5.89 ± 0.066	1.544AspMet: 1.544 ± 0.037	1.394AspAsn: 1.394 ± 0.037	3.601AspPro: 3.601 ± 0.054	1.946AspGln: 1.946 ± 0.035	4.742AspArg: 4.742 ± 0.067	2.475AspSer: 2.475 ± 0.048	2.246AspThr: 2.246 ± 0.046	3.926AspVal: 3.926 ± 0.062	1.044AspTrp: 1.044 ± 0.032	1.676AspTyr: 1.676 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	7.735GluAla: 7.735 ± 0.104	0.342GluCys: 0.342 ± 0.017	2.815GluAsp: 2.815 ± 0.054	3.448GluGlu: 3.448 ± 0.065	1.492GluPhe: 1.492 ± 0.033	4.78GluGly: 4.78 ± 0.061	1.109GluHis: 1.109 ± 0.028	3.049GluIle: 3.049 ± 0.057	2.152GluLys: 2.152 ± 0.059	5.095GluLeu: 5.095 ± 0.07	1.536GluMet: 1.536 ± 0.039	1.427GluAsn: 1.427 ± 0.037	2.55GluPro: 2.55 ± 0.046	2.335GluGln: 2.335 ± 0.045	4.996GluArg: 4.996 ± 0.069	2.374GluSer: 2.374 ± 0.043	2.92GluThr: 2.92 ± 0.047	3.588GluVal: 3.588 ± 0.056	0.77GluTrp: 0.77 ± 0.025	0.978GluTyr: 0.978 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	4.594PheAla: 4.594 ± 0.07	0.367PheCys: 0.367 ± 0.018	2.543PheAsp: 2.543 ± 0.052	1.962PheGlu: 1.962 ± 0.038	1.265PhePhe: 1.265 ± 0.037	3.458PheGly: 3.458 ± 0.055	0.752PheHis: 0.752 ± 0.026	1.544PheIle: 1.544 ± 0.035	0.936PheLys: 0.936 ± 0.029	3.181PheLeu: 3.181 ± 0.054	0.797PheMet: 0.797 ± 0.024	1.084PheAsn: 1.084 ± 0.032	1.608PhePro: 1.608 ± 0.039	0.982PheGln: 0.982 ± 0.03	2.274PheArg: 2.274 ± 0.045	2.205PheSer: 2.205 ± 0.045	1.989PheThr: 1.989 ± 0.043	2.418PheVal: 2.418 ± 0.043	0.51PheTrp: 0.51 ± 0.022	0.913PheTyr: 0.913 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	9.605GlyAla: 9.605 ± 0.114	0.823GlyCys: 0.823 ± 0.026	4.676GlyAsp: 4.676 ± 0.068	4.845GlyGlu: 4.845 ± 0.062	3.573GlyPhe: 3.573 ± 0.055	8.111GlyGly: 8.111 ± 0.141	1.893GlyHis: 1.893 ± 0.041	4.492GlyIle: 4.492 ± 0.075	3.426GlyLys: 3.426 ± 0.058	8.589GlyLeu: 8.589 ± 0.091	2.399GlyMet: 2.399 ± 0.051	2.338GlyAsn: 2.338 ± 0.056	3.619GlyPro: 3.619 ± 0.065	3.197GlyGln: 3.197 ± 0.052	6.59GlyArg: 6.59 ± 0.073	5.051GlySer: 5.051 ± 0.082	4.461GlyThr: 4.461 ± 0.075	6.151GlyVal: 6.151 ± 0.078	1.584GlyTrp: 1.584 ± 0.04	2.353GlyTyr: 2.353 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.32HisAla: 2.32 ± 0.053	0.236HisCys: 0.236 ± 0.015	1.306HisAsp: 1.306 ± 0.034	1.03HisGlu: 1.03 ± 0.031	0.876HisPhe: 0.876 ± 0.028	2.088HisGly: 2.088 ± 0.041	0.637HisHis: 0.637 ± 0.022	1.037HisIle: 1.037 ± 0.033	0.52HisLys: 0.52 ± 0.021	2.022HisLeu: 2.022 ± 0.041	0.532HisMet: 0.532 ± 0.023	0.459HisAsn: 0.459 ± 0.017	1.314HisPro: 1.314 ± 0.033	0.6HisGln: 0.6 ± 0.021	1.477HisArg: 1.477 ± 0.035	1.039HisSer: 1.039 ± 0.03	0.615HisThr: 0.615 ± 0.02	1.624HisVal: 1.624 ± 0.039	0.38HisTrp: 0.38 ± 0.018	0.608HisTyr: 0.608 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	7.544IleAla: 7.544 ± 0.08	0.52IleCys: 0.52 ± 0.024	4.08IleAsp: 4.08 ± 0.06	3.477IleGlu: 3.477 ± 0.054	1.734IlePhe: 1.734 ± 0.038	5.017IleGly: 5.017 ± 0.064	0.944IleHis: 0.944 ± 0.032	2.495IleIle: 2.495 ± 0.051	1.378IleLys: 1.378 ± 0.038	4.339IleLeu: 4.339 ± 0.07	1.058IleMet: 1.058 ± 0.027	1.47IleAsn: 1.47 ± 0.037	2.293IlePro: 2.293 ± 0.05	1.252IleGln: 1.252 ± 0.035	3.287IleArg: 3.287 ± 0.055	3.004IleSer: 3.004 ± 0.054	2.519IleThr: 2.519 ± 0.054	3.924IleVal: 3.924 ± 0.055	0.653IleTrp: 0.653 ± 0.025	1.097IleTyr: 1.097 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	4.414LysAla: 4.414 ± 0.082	0.183LysCys: 0.183 ± 0.012	1.786LysAsp: 1.786 ± 0.043	1.442LysGlu: 1.442 ± 0.038	0.834LysPhe: 0.834 ± 0.025	2.908LysGly: 2.908 ± 0.05	0.576LysHis: 0.576 ± 0.024	1.637LysIle: 1.637 ± 0.035	1.198LysLys: 1.198 ± 0.039	3.361LysLeu: 3.361 ± 0.057	0.764LysMet: 0.764 ± 0.026	0.766LysAsn: 0.766 ± 0.023	2.067LysPro: 2.067 ± 0.048	1.015LysGln: 1.015 ± 0.029	2.297LysArg: 2.297 ± 0.048	1.71LysSer: 1.71 ± 0.039	1.628LysThr: 1.628 ± 0.047	2.36LysVal: 2.36 ± 0.048	0.42LysTrp: 0.42 ± 0.019	0.612LysTyr: 0.612 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	13.187LeuAla: 13.187 ± 0.136	0.898LeuCys: 0.898 ± 0.027	5.921LeuAsp: 5.921 ± 0.074	4.952LeuGlu: 4.952 ± 0.075	3.728LeuPhe: 3.728 ± 0.073	8.052LeuGly: 8.052 ± 0.09	1.92LeuHis: 1.92 ± 0.042	5.241LeuIle: 5.241 ± 0.071	3.228LeuLys: 3.228 ± 0.051	10.045LeuLeu: 10.045 ± 0.138	2.325LeuMet: 2.325 ± 0.047	2.667LeuAsn: 2.667 ± 0.047	5.697LeuPro: 5.697 ± 0.073	2.716LeuGln: 2.716 ± 0.052	6.98LeuArg: 6.98 ± 0.081	6.635LeuSer: 6.635 ± 0.077	5.624LeuThr: 5.624 ± 0.075	6.605LeuVal: 6.605 ± 0.08	1.262LeuTrp: 1.262 ± 0.036	1.989LeuTyr: 1.989 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	3.442MetAla: 3.442 ± 0.055	0.151MetCys: 0.151 ± 0.01	1.246MetAsp: 1.246 ± 0.032	1.166MetGlu: 1.166 ± 0.034	0.701MetPhe: 0.701 ± 0.024	2.005MetGly: 2.005 ± 0.045	0.45MetHis: 0.45 ± 0.02	1.448MetIle: 1.448 ± 0.038	0.998MetLys: 0.998 ± 0.029	2.8MetLeu: 2.8 ± 0.051	0.715MetMet: 0.715 ± 0.025	0.746MetAsn: 0.746 ± 0.023	1.489MetPro: 1.489 ± 0.033	0.805MetGln: 0.805 ± 0.029	1.857MetArg: 1.857 ± 0.039	1.594MetSer: 1.594 ± 0.035	1.839MetThr: 1.839 ± 0.043	1.65MetVal: 1.65 ± 0.04	0.219MetTrp: 0.219 ± 0.013	0.244MetTyr: 0.244 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.247AsnAla: 3.247 ± 0.055	0.262AsnCys: 0.262 ± 0.015	1.426AsnAsp: 1.426 ± 0.044	1.216AsnGlu: 1.216 ± 0.031	0.97AsnPhe: 0.97 ± 0.032	2.653AsnGly: 2.653 ± 0.06	0.516AsnHis: 0.516 ± 0.02	1.508AsnIle: 1.508 ± 0.036	0.711AsnLys: 0.711 ± 0.027	2.535AsnLeu: 2.535 ± 0.052	0.644AsnMet: 0.644 ± 0.023	0.773AsnAsn: 0.773 ± 0.033	1.809AsnPro: 1.809 ± 0.041	0.849AsnGln: 0.849 ± 0.026	1.913AsnArg: 1.913 ± 0.043	1.399AsnSer: 1.399 ± 0.035	0.988AsnThr: 0.988 ± 0.034	1.962AsnVal: 1.962 ± 0.045	0.439AsnTrp: 0.439 ± 0.02	0.725AsnTyr: 0.725 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	6.644ProAla: 6.644 ± 0.083	0.346ProCys: 0.346 ± 0.015	3.643ProAsp: 3.643 ± 0.059	3.496ProGlu: 3.496 ± 0.054	1.937ProPhe: 1.937 ± 0.044	4.601ProGly: 4.601 ± 0.07	1.097ProHis: 1.097 ± 0.034	2.554ProIle: 2.554 ± 0.048	1.59ProLys: 1.59 ± 0.041	5.054ProLeu: 5.054 ± 0.068	1.278ProMet: 1.278 ± 0.034	1.355ProAsn: 1.355 ± 0.034	2.712ProPro: 2.712 ± 0.068	1.739ProGln: 1.739 ± 0.036	3.003ProArg: 3.003 ± 0.063	2.941ProSer: 2.941 ± 0.049	2.463ProThr: 2.463 ± 0.047	4.256ProVal: 4.256 ± 0.058	0.71ProTrp: 0.71 ± 0.022	1.132ProTyr: 1.132 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	4.481GlnAla: 4.481 ± 0.07	0.265GlnCys: 0.265 ± 0.016	1.597GlnAsp: 1.597 ± 0.041	1.549GlnGlu: 1.549 ± 0.041	1.146GlnPhe: 1.146 ± 0.033	2.736GlnGly: 2.736 ± 0.05	0.669GlnHis: 0.669 ± 0.024	1.941GlnIle: 1.941 ± 0.042	1.072GlnLys: 1.072 ± 0.032	3.373GlnLeu: 3.373 ± 0.047	0.961GlnMet: 0.961 ± 0.028	0.83GlnAsn: 0.83 ± 0.023	1.89GlnPro: 1.89 ± 0.042	1.359GlnGln: 1.359 ± 0.042	2.625GlnArg: 2.625 ± 0.054	1.927GlnSer: 1.927 ± 0.04	1.639GlnThr: 1.639 ± 0.04	2.255GlnVal: 2.255 ± 0.047	0.495GlnTrp: 0.495 ± 0.019	0.639GlnTyr: 0.639 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	8.379ArgAla: 8.379 ± 0.094	0.559ArgCys: 0.559 ± 0.025	4.182ArgAsp: 4.182 ± 0.066	4.046ArgGlu: 4.046 ± 0.057	3.093ArgPhe: 3.093 ± 0.052	5.069ArgGly: 5.069 ± 0.072	1.793ArgHis: 1.793 ± 0.046	4.046ArgIle: 4.046 ± 0.066	2.263ArgLys: 2.263 ± 0.047	8.089ArgLeu: 8.089 ± 0.096	1.995ArgMet: 1.995 ± 0.04	1.943ArgAsn: 1.943 ± 0.041	3.724ArgPro: 3.724 ± 0.061	2.819ArgGln: 2.819 ± 0.056	5.613ArgArg: 5.613 ± 0.085	4.005ArgSer: 4.005 ± 0.058	3.456ArgThr: 3.456 ± 0.054	4.651ArgVal: 4.651 ± 0.062	1.237ArgTrp: 1.237 ± 0.031	1.884ArgTyr: 1.884 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	6.851SerAla: 6.851 ± 0.086	0.512SerCys: 0.512 ± 0.025	3.136SerAsp: 3.136 ± 0.058	2.709SerGlu: 2.709 ± 0.046	2.365SerPhe: 2.365 ± 0.051	5.634SerGly: 5.634 ± 0.082	1.112SerHis: 1.112 ± 0.031	2.908SerIle: 2.908 ± 0.049	1.613SerLys: 1.613 ± 0.035	5.518SerLeu: 5.518 ± 0.075	1.332SerMet: 1.332 ± 0.034	1.429SerAsn: 1.429 ± 0.036	3.015SerPro: 3.015 ± 0.049	1.694SerGln: 1.694 ± 0.042	3.801SerArg: 3.801 ± 0.059	3.175SerSer: 3.175 ± 0.059	2.621SerThr: 2.621 ± 0.051	3.755SerVal: 3.755 ± 0.061	0.889SerTrp: 0.889 ± 0.028	1.484SerTyr: 1.484 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	6.076ThrAla: 6.076 ± 0.075	0.42ThrCys: 0.42 ± 0.021	2.807ThrAsp: 2.807 ± 0.058	2.289ThrGlu: 2.289 ± 0.043	1.592ThrPhe: 1.592 ± 0.04	5.173ThrGly: 5.173 ± 0.083	0.988ThrHis: 0.988 ± 0.028	2.79ThrIle: 2.79 ± 0.054	1.345ThrLys: 1.345 ± 0.036	5.539ThrLeu: 5.539 ± 0.071	1.126ThrMet: 1.126 ± 0.033	1.321ThrAsn: 1.321 ± 0.037	3.216ThrPro: 3.216 ± 0.055	1.558ThrGln: 1.558 ± 0.04	3.251ThrArg: 3.251 ± 0.061	2.653ThrSer: 2.653 ± 0.053	2.38ThrThr: 2.38 ± 0.051	3.9ThrVal: 3.9 ± 0.064	0.58ThrTrp: 0.58 ± 0.022	1.066ThrTyr: 1.066 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	8.78ValAla: 8.78 ± 0.094	0.529ValCys: 0.529 ± 0.021	4.162ValAsp: 4.162 ± 0.055	4.684ValGlu: 4.684 ± 0.059	2.012ValPhe: 2.012 ± 0.043	5.48ValGly: 5.48 ± 0.078	1.382ValHis: 1.382 ± 0.032	3.698ValIle: 3.698 ± 0.061	2.229ValLys: 2.229 ± 0.039	6.025ValLeu: 6.025 ± 0.079	1.731ValMet: 1.731 ± 0.041	2.071ValAsn: 2.071 ± 0.045	3.725ValPro: 3.725 ± 0.055	2.224ValGln: 2.224 ± 0.042	5.001ValArg: 5.001 ± 0.059	4.066ValSer: 4.066 ± 0.063	4.412ValThr: 4.412 ± 0.075	4.948ValVal: 4.948 ± 0.073	0.723ValTrp: 0.723 ± 0.024	1.33ValTyr: 1.33 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.371TrpAla: 1.371 ± 0.038	0.11TrpCys: 0.11 ± 0.01	0.722TrpAsp: 0.722 ± 0.027	0.639TrpGlu: 0.639 ± 0.025	0.536TrpPhe: 0.536 ± 0.021	0.98TrpGly: 0.98 ± 0.025	0.357TrpHis: 0.357 ± 0.017	0.694TrpIle: 0.694 ± 0.025	0.491TrpLys: 0.491 ± 0.019	1.713TrpLeu: 1.713 ± 0.041	0.386TrpMet: 0.386 ± 0.017	0.469TrpAsn: 0.469 ± 0.022	0.745TrpPro: 0.745 ± 0.027	0.591TrpGln: 0.591 ± 0.023	1.351TrpArg: 1.351 ± 0.036	0.85TrpSer: 0.85 ± 0.029	0.821TrpThr: 0.821 ± 0.026	0.795TrpVal: 0.795 ± 0.027	0.247TrpTrp: 0.247 ± 0.015	0.298TrpTyr: 0.298 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.582TyrAla: 2.582 ± 0.047	0.275TyrCys: 0.275 ± 0.017	1.553TyrAsp: 1.553 ± 0.039	1.185TyrGlu: 1.185 ± 0.032	0.86TyrPhe: 0.86 ± 0.025	2.134TyrGly: 2.134 ± 0.052	0.532TyrHis: 0.532 ± 0.021	0.866TyrIle: 0.866 ± 0.024	0.631TyrLys: 0.631 ± 0.023	2.133TyrLeu: 2.133 ± 0.041	0.462TyrMet: 0.462 ± 0.018	0.659TyrAsn: 0.659 ± 0.027	1.055TyrPro: 1.055 ± 0.032	0.748TyrGln: 0.748 ± 0.026	1.962TyrArg: 1.962 ± 0.038	1.336TyrSer: 1.336 ± 0.039	0.938TyrThr: 0.938 ± 0.028	1.543TyrVal: 1.543 ± 0.036	0.35TyrTrp: 0.35 ± 0.016	0.621TyrTyr: 0.621 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4048 proteins (1252722 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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