Aspergillus tubingensis (strain CBS 134.48)
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12319 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L9MX80|A0A1L9MX80_ASPTC NAD(P)-bd_dom domain-containing protein OS=Aspergillus tubingensis (strain CBS 134.48) OX=767770 GN=ASPTUDRAFT_125518 PE=4 SV=1
MM1 pKa = 6.89 KK2 pKa = 9.88 TYY4 pKa = 10.55 GIWSTLATLGSFTLSSAKK22 pKa = 9.29 TLNFVAHH29 pKa = 7.12 EE30 pKa = 4.56 DD31 pKa = 3.72 DD32 pKa = 5.65 DD33 pKa = 5.68 LLFLSPDD40 pKa = 4.47 LIRR43 pKa = 11.84 DD44 pKa = 3.62 ILSGEE49 pKa = 4.14 PLRR52 pKa = 11.84 TVFLTAGDD60 pKa = 3.85 AGMGSDD66 pKa = 3.55 YY67 pKa = 10.75 WNSRR71 pKa = 11.84 EE72 pKa = 4.11 DD73 pKa = 3.59 GSRR76 pKa = 11.84 AAYY79 pKa = 10.58 ARR81 pKa = 11.84 MAVASNNWDD90 pKa = 3.14 EE91 pKa = 4.21 DD92 pKa = 4.09 VIYY95 pKa = 11.19 VGDD98 pKa = 3.94 KK99 pKa = 10.37 TISLYY104 pKa = 10.02 TLQDD108 pKa = 3.76 DD109 pKa = 5.22 DD110 pKa = 5.76 DD111 pKa = 5.1 ISLAFMHH118 pKa = 6.84 LPDD121 pKa = 4.93 GNMDD125 pKa = 3.31 GTGFGGTDD133 pKa = 3.76 QNDD136 pKa = 3.81 SLQKK140 pKa = 10.82 LWDD143 pKa = 3.55 GDD145 pKa = 3.14 IDD147 pKa = 4.19 VIRR150 pKa = 11.84 TIDD153 pKa = 3.57 GSGVEE158 pKa = 4.13 YY159 pKa = 10.3 TRR161 pKa = 11.84 DD162 pKa = 3.39 EE163 pKa = 4.31 LLEE166 pKa = 4.35 TIAALIDD173 pKa = 4.57 DD174 pKa = 5.25 FDD176 pKa = 5.85 PDD178 pKa = 3.61 EE179 pKa = 4.89 VKK181 pKa = 10.28 TGDD184 pKa = 3.6 YY185 pKa = 11.43 VNDD188 pKa = 4.35 FGDD191 pKa = 4.38 GDD193 pKa = 4.1 HH194 pKa = 7.19 SDD196 pKa = 4.11 HH197 pKa = 6.56 YY198 pKa = 9.99 ATGYY202 pKa = 10.41 FVDD205 pKa = 4.5 NALQQADD212 pKa = 3.87 SDD214 pKa = 4.31 ADD216 pKa = 3.51 LTGYY220 pKa = 10.02 YY221 pKa = 10.2 GYY223 pKa = 9.34 PIQNMDD229 pKa = 3.52 VNLDD233 pKa = 3.65 DD234 pKa = 6.3 SDD236 pKa = 5.69 IEE238 pKa = 4.29 DD239 pKa = 3.65 KK240 pKa = 11.42 TNIFYY245 pKa = 10.61 EE246 pKa = 4.16 YY247 pKa = 10.63 AGYY250 pKa = 8.18 DD251 pKa = 3.49 TATCATDD258 pKa = 3.84 DD259 pKa = 3.99 ACSSRR264 pKa = 11.84 PEE266 pKa = 4.27 SAWLQRR272 pKa = 11.84 EE273 pKa = 4.24 YY274 pKa = 11.31 EE275 pKa = 4.13 LL276 pKa = 5.67
Molecular weight: 30.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.605
IPC_protein 3.656
Toseland 3.414
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.643
Rodwell 3.478
Grimsley 3.325
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.088
Thurlkill 3.478
EMBOSS 3.643
Sillero 3.783
Patrickios 0.985
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.736
Protein with the highest isoelectric point:
>tr|A0A1L9N8R7|A0A1L9N8R7_ASPTC Uncharacterized protein OS=Aspergillus tubingensis (strain CBS 134.48) OX=767770 GN=ASPTUDRAFT_927216 PE=4 SV=1
MM1 pKa = 7.56 IPTHH5 pKa = 4.89 STLRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 SAVRR15 pKa = 11.84 SRR17 pKa = 11.84 KK18 pKa = 9.78 ASVIRR23 pKa = 11.84 RR24 pKa = 11.84 NFKK27 pKa = 10.39 VVTTSGMIRR36 pKa = 11.84 RR37 pKa = 11.84 SHH39 pKa = 5.98 SPFWLLRR46 pKa = 11.84 LPVSLIFQVV55 pKa = 3.28
Molecular weight: 6.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.544
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.281
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.178
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12318
1
12319
5835640
49
6265
473.7
52.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.387 ± 0.021
1.329 ± 0.009
5.603 ± 0.014
6.074 ± 0.021
3.755 ± 0.013
6.79 ± 0.02
2.466 ± 0.01
4.995 ± 0.016
4.411 ± 0.017
9.17 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.198 ± 0.008
3.591 ± 0.011
6.023 ± 0.024
3.996 ± 0.014
6.104 ± 0.019
8.315 ± 0.025
6.055 ± 0.015
6.265 ± 0.016
1.521 ± 0.008
2.953 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here