Capybara microvirus Cap1_SP_84
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W408|A0A4P8W408_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_84 OX=2584795 PE=4 SV=1
MM1 pKa = 7.54 LVTKK5 pKa = 10.51 QFTDD9 pKa = 3.57 EE10 pKa = 4.22 SRR12 pKa = 11.84 CAQLIGSHH20 pKa = 5.53 NHH22 pKa = 6.92 DD23 pKa = 4.45 DD24 pKa = 3.64 SLIYY28 pKa = 10.72 VPSKK32 pKa = 10.51 FMKK35 pKa = 10.48 GDD37 pKa = 3.6 VEE39 pKa = 4.65 SVSLNSDD46 pKa = 2.83 IVGYY50 pKa = 10.92 ASDD53 pKa = 4.81 FSLEE57 pKa = 4.06 SLQAAGVDD65 pKa = 3.74 PGSIAPNLSTDD76 pKa = 3.4 RR77 pKa = 11.84 TRR79 pKa = 11.84 PNMDD83 pKa = 3.44 KK84 pKa = 10.85 EE85 pKa = 4.25 VDD87 pKa = 3.46 LSSFAEE93 pKa = 4.34 SLSSLQVDD101 pKa = 3.96 DD102 pKa = 5.7 DD103 pKa = 4.33 LPEE106 pKa = 3.84
Molecular weight: 11.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.264
IPC2_protein 4.024
IPC_protein 3.986
Toseland 3.77
ProMoST 4.113
Dawson 3.986
Bjellqvist 4.202
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.681
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.355
Thurlkill 3.834
EMBOSS 3.948
Sillero 4.113
Patrickios 3.719
IPC_peptide 3.973
IPC2_peptide 4.088
IPC2.peptide.svr19 4.033
Protein with the highest isoelectric point:
>tr|A0A4P8W4B0|A0A4P8W4B0_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_84 OX=2584795 PE=4 SV=1
MM1 pKa = 7.11 QATITFSNRR10 pKa = 11.84 KK11 pKa = 9.52 KK12 pKa = 10.86 EE13 pKa = 4.05 DD14 pKa = 3.65 FNPLFDD20 pKa = 4.18 RR21 pKa = 11.84 EE22 pKa = 4.61 KK23 pKa = 9.84 ITIQNRR29 pKa = 11.84 RR30 pKa = 11.84 LLKK33 pKa = 10.35 FQAAVQGGYY42 pKa = 6.58 EE43 pKa = 3.58 TRR45 pKa = 11.84 MYY47 pKa = 10.98 YY48 pKa = 10.32 EE49 pKa = 4.35 YY50 pKa = 10.99 LNCIDD55 pKa = 4.02 KK56 pKa = 11.14 GGFVAFVTLTYY67 pKa = 10.92 NNKK70 pKa = 9.91 ALYY73 pKa = 9.57 HH74 pKa = 6.27 FYY76 pKa = 10.97 GLPVPDD82 pKa = 4.16 SRR84 pKa = 11.84 NLRR87 pKa = 11.84 YY88 pKa = 9.62 LLHH91 pKa = 7.34 DD92 pKa = 3.47 SYY94 pKa = 11.16 FAHH97 pKa = 6.51 EE98 pKa = 4.62 CARR101 pKa = 11.84 SGYY104 pKa = 7.86 NFKK107 pKa = 11.17 YY108 pKa = 10.4 FITTEE113 pKa = 3.93 LGEE116 pKa = 4.56 GKK118 pKa = 10.31 GKK120 pKa = 10.51 RR121 pKa = 11.84 GFANNPHH128 pKa = 5.38 YY129 pKa = 10.8 HH130 pKa = 6.46 CLLYY134 pKa = 8.03 FTPSTPEE141 pKa = 3.52 YY142 pKa = 10.25 NRR144 pKa = 11.84 FFITRR149 pKa = 11.84 YY150 pKa = 8.92 VDD152 pKa = 4.57 LIKK155 pKa = 10.69 KK156 pKa = 8.35 YY157 pKa = 8.1 WQGDD161 pKa = 3.37 NGEE164 pKa = 4.56 DD165 pKa = 2.88 WNAAKK170 pKa = 10.24 FGHH173 pKa = 5.99 VEE175 pKa = 3.77 PGDD178 pKa = 3.33 NYY180 pKa = 11.32 GIVNSSSAFSYY191 pKa = 9.03 VSKK194 pKa = 10.99 YY195 pKa = 6.57 VTKK198 pKa = 10.67 SSTDD202 pKa = 2.77 IHH204 pKa = 6.94 RR205 pKa = 11.84 EE206 pKa = 3.74 RR207 pKa = 11.84 IVAARR212 pKa = 11.84 SKK214 pKa = 10.21 EE215 pKa = 4.02 YY216 pKa = 9.41 WSKK219 pKa = 11.0 ILDD222 pKa = 3.9 PCSSEE227 pKa = 5.02 FKK229 pKa = 11.16 VLDD232 pKa = 3.43 IKK234 pKa = 11.49 LNDD237 pKa = 4.86 LIKK240 pKa = 10.82 ADD242 pKa = 3.28 INKK245 pKa = 8.97 YY246 pKa = 10.15 RR247 pKa = 11.84 NLFGTRR253 pKa = 11.84 VRR255 pKa = 11.84 CSHH258 pKa = 6.38 GFGQIDD264 pKa = 3.65 EE265 pKa = 4.45 TLLTFIHH272 pKa = 7.09 DD273 pKa = 3.87 KK274 pKa = 10.74 PHH276 pKa = 7.12 IKK278 pKa = 9.17 LTSNGKK284 pKa = 8.25 LTYY287 pKa = 10.18 KK288 pKa = 10.45 PLPLYY293 pKa = 10.4 NYY295 pKa = 8.84 RR296 pKa = 11.84 KK297 pKa = 9.79 LYY299 pKa = 9.99 MYY301 pKa = 10.91 VDD303 pKa = 3.47 EE304 pKa = 4.65 NKK306 pKa = 10.25 CYY308 pKa = 10.52 KK309 pKa = 10.54 LNEE312 pKa = 4.04 AGIEE316 pKa = 4.18 LKK318 pKa = 10.62 CRR320 pKa = 11.84 QVSSALKK327 pKa = 10.83 DD328 pKa = 3.47 MADD331 pKa = 3.57 NAQLICRR338 pKa = 11.84 NTLSCCEE345 pKa = 4.12 VFSLLPSPLDD355 pKa = 3.34 LSTYY359 pKa = 9.78 IQQCNCKK366 pKa = 10.09 LGEE369 pKa = 4.17 SPLFHH374 pKa = 7.86 DD375 pKa = 4.07 YY376 pKa = 11.6 ALYY379 pKa = 9.59 TLLYY383 pKa = 8.8 KK384 pKa = 10.87 DD385 pKa = 3.92 RR386 pKa = 11.84 MYY388 pKa = 10.78 PSWPSGEE395 pKa = 4.73 FFDD398 pKa = 5.79 EE399 pKa = 4.32 PDD401 pKa = 4.15 IDD403 pKa = 3.79 PLSYY407 pKa = 10.03 IRR409 pKa = 11.84 QVSSRR414 pKa = 11.84 VDD416 pKa = 3.0 KK417 pKa = 11.23 NSYY420 pKa = 9.18 FFCSDD425 pKa = 3.63 CAHH428 pKa = 7.26 LLPYY432 pKa = 9.89 KK433 pKa = 8.98 YY434 pKa = 9.96 HH435 pKa = 5.95 SRR437 pKa = 11.84 FRR439 pKa = 11.84 FNLPFFSAIDD449 pKa = 3.68 SVLSHH454 pKa = 6.21 HH455 pKa = 7.44 TIQTDD460 pKa = 3.29 EE461 pKa = 4.82 KK462 pKa = 9.47 LTQEE466 pKa = 3.76 HH467 pKa = 6.28 EE468 pKa = 3.78 LRR470 pKa = 11.84 KK471 pKa = 9.89 RR472 pKa = 11.84 IRR474 pKa = 11.84 NSVLAQRR481 pKa = 11.84 KK482 pKa = 8.05 PFLFHH487 pKa = 7.16 SYY489 pKa = 10.38 KK490 pKa = 10.83
Molecular weight: 57.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.067
IPC2_protein 8.156
IPC_protein 7.966
Toseland 7.849
ProMoST 8.565
Dawson 8.726
Bjellqvist 8.916
Wikipedia 8.741
Rodwell 8.77
Grimsley 7.892
Solomon 8.785
Lehninger 8.77
Nozaki 8.99
DTASelect 8.712
Thurlkill 8.756
EMBOSS 8.873
Sillero 9.019
Patrickios 3.821
IPC_peptide 8.77
IPC2_peptide 7.805
IPC2.peptide.svr19 8.002
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1678
78
603
335.6
38.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.555 ± 1.232
1.192 ± 0.581
6.436 ± 0.543
5.959 ± 0.879
5.662 ± 0.639
5.304 ± 0.724
2.086 ± 0.52
4.41 ± 0.26
5.9 ± 0.636
9.178 ± 0.368
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.907 ± 0.328
7.032 ± 1.056
3.456 ± 0.508
4.529 ± 1.176
5.304 ± 0.482
8.522 ± 0.66
4.768 ± 0.601
5.662 ± 0.848
1.073 ± 0.287
5.066 ± 1.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here