Aeromonas phage AhyVDH1
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6H1NP77|A0A6H1NP77_9CAUD Uncharacterized protein OS=Aeromonas phage AhyVDH1 OX=2724309 GN=AhyVDH1_056 PE=4 SV=1
MM1 pKa = 7.34 NMKK4 pKa = 10.24 NLDD7 pKa = 3.72 HH8 pKa = 5.74 QQCSILLGFAEE19 pKa = 4.65 EE20 pKa = 3.93 NWEE23 pKa = 3.97 QFVAHH28 pKa = 5.75 VAGHH32 pKa = 5.93 NGITEE37 pKa = 4.11 EE38 pKa = 3.98 EE39 pKa = 4.37 AEE41 pKa = 4.63 AEE43 pKa = 4.08 AEE45 pKa = 4.57 DD46 pKa = 3.8 IVEE49 pKa = 4.27 ALGEE53 pKa = 4.25 GANGG57 pKa = 3.39
Molecular weight: 6.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.156
IPC2_protein 4.151
IPC_protein 3.961
Toseland 3.846
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.757
Rodwell 3.821
Grimsley 3.77
Solomon 3.884
Lehninger 3.834
Nozaki 4.037
DTASelect 4.062
Thurlkill 3.859
EMBOSS 3.77
Sillero 4.075
Patrickios 3.083
IPC_peptide 3.897
IPC2_peptide 4.062
IPC2.peptide.svr19 3.986
Protein with the highest isoelectric point:
>tr|A0A6H1NP78|A0A6H1NP78_9CAUD Baseplate assembly protein V OS=Aeromonas phage AhyVDH1 OX=2724309 GN=AhyVDH1_020 PE=4 SV=1
MM1 pKa = 7.19 TRR3 pKa = 11.84 RR4 pKa = 11.84 PVPIVATSIQTGEE17 pKa = 4.06 VFNYY21 pKa = 8.95 PSRR24 pKa = 11.84 AAAAEE29 pKa = 4.1 SGGFCAATITLCIRR43 pKa = 11.84 GIVPSHH49 pKa = 6.69 AGFKK53 pKa = 9.71 FHH55 pKa = 7.1 ATAKK59 pKa = 9.76 LDD61 pKa = 3.54 PPRR64 pKa = 11.84 RR65 pKa = 11.84 PKK67 pKa = 10.35 LAAKK71 pKa = 9.97 VKK73 pKa = 8.67 EE74 pKa = 3.99 LRR76 pKa = 11.84 ATGLSYY82 pKa = 11.49 AKK84 pKa = 9.15 TAKK87 pKa = 10.6 ALGISEE93 pKa = 4.09 STAYY97 pKa = 10.11 YY98 pKa = 9.4 HH99 pKa = 6.86 ARR101 pKa = 11.84 NRR103 pKa = 11.84 AA104 pKa = 3.05
Molecular weight: 11.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.794
IPC_protein 10.394
Toseland 10.584
ProMoST 10.233
Dawson 10.716
Bjellqvist 10.394
Wikipedia 10.891
Rodwell 11.067
Grimsley 10.774
Solomon 10.774
Lehninger 10.76
Nozaki 10.584
DTASelect 10.394
Thurlkill 10.599
EMBOSS 10.979
Sillero 10.643
Patrickios 10.818
IPC_peptide 10.789
IPC2_peptide 9.37
IPC2.peptide.svr19 8.511
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
59
0
59
11512
44
869
195.1
21.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.954 ± 0.616
1.173 ± 0.127
5.299 ± 0.283
6.246 ± 0.283
2.936 ± 0.17
8.0 ± 0.376
2.163 ± 0.209
4.917 ± 0.194
5.837 ± 0.266
8.383 ± 0.226
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.162 ± 0.166
3.37 ± 0.243
4.421 ± 0.299
3.952 ± 0.231
6.055 ± 0.335
5.881 ± 0.235
6.194 ± 0.263
7.149 ± 0.308
1.581 ± 0.136
2.328 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here