Daphnia pulex (Water flea)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Crustacea; Branchiopoda; Phyllopoda; Diplostraca; Cladocera; Anomopoda; Daphniidae; Daphnia

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9GEE1|E9GEE1_DAPPU PBPe domain-containing protein OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_101737 PE=4 SV=1
MM1 pKa = 7.4SSQVSFLVLMAMAVVALTGTLGSCSIIIRR30 pKa = 11.84SASAGDD36 pKa = 4.07YY37 pKa = 11.13YY38 pKa = 11.34DD39 pKa = 5.93LNQQQLVLSPEE50 pKa = 3.37IRR52 pKa = 11.84AKK54 pKa = 9.93VVRR57 pKa = 11.84PYY59 pKa = 11.51APLQNEE65 pKa = 4.1LTEE68 pKa = 4.21EE69 pKa = 3.96NLAKK73 pKa = 10.59VEE75 pKa = 4.12EE76 pKa = 4.56LTTDD80 pKa = 3.2VPTDD84 pKa = 3.46EE85 pKa = 4.7TTDD88 pKa = 3.33ATTDD92 pKa = 3.27APTNAPTDD100 pKa = 3.96TPTDD104 pKa = 3.63TPTDD108 pKa = 3.78EE109 pKa = 4.72PTDD112 pKa = 3.94KK113 pKa = 9.98PTDD116 pKa = 3.27AHH118 pKa = 6.35TDD120 pKa = 3.35APTDD124 pKa = 3.54APTNAPTDD132 pKa = 3.75APTDD136 pKa = 3.73APTDD140 pKa = 3.66APTNAPTNAPTDD152 pKa = 3.34AHH154 pKa = 6.4TDD156 pKa = 3.13APTNVPTDD164 pKa = 3.64APTDD168 pKa = 3.74APTEE172 pKa = 4.15APTNVPTDD180 pKa = 3.64APTDD184 pKa = 3.75APTDD188 pKa = 3.73APTDD192 pKa = 3.66APTNAPTDD200 pKa = 3.77APTQAPTDD208 pKa = 3.94APTQAPTDD216 pKa = 3.79APTNAPTNAPTDD228 pKa = 3.34AHH230 pKa = 6.33TDD232 pKa = 3.4APTDD236 pKa = 3.63APTQAPTDD244 pKa = 3.79APTNAPTDD252 pKa = 3.75APTDD256 pKa = 3.66APTDD260 pKa = 3.73EE261 pKa = 5.19PPNTTADD268 pKa = 3.74PTTDD272 pKa = 3.16TTTEE276 pKa = 4.02TSTDD280 pKa = 3.22TSTDD284 pKa = 2.76ATTATTVSTTTTTTTTTTAATVSTTTPVTEE314 pKa = 4.39ASTTTSVTTAPFSSSTATISSTSARR339 pKa = 11.84TTTSAPSSGNIFTRR353 pKa = 11.84VQAHH357 pKa = 6.41LEE359 pKa = 4.6FIQSHH364 pKa = 5.24TKK366 pKa = 9.67VSSPADD372 pKa = 3.42CSSTTNGTEE381 pKa = 3.9PTTTPGPTTSTTQKK395 pKa = 8.96ITTTSTRR402 pKa = 11.84KK403 pKa = 6.25TTTSTTTASTTNQSVTFSCAGKK425 pKa = 10.77ANGNNPNPASSCSNTFYY442 pKa = 11.25ACSNGNACLFNCPSNLVYY460 pKa = 10.5RR461 pKa = 11.84EE462 pKa = 4.1EE463 pKa = 5.09IGVCDD468 pKa = 3.9YY469 pKa = 10.42PSNVAGCYY477 pKa = 9.32

Molecular weight: 48.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9I6K4|E9I6K4_DAPPU Uncharacterized protein OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_124402 PE=4 SV=1
AA1 pKa = 7.54QAQAQASPRR10 pKa = 11.84FAKK13 pKa = 9.93VRR15 pKa = 11.84QGSPRR20 pKa = 11.84FAKK23 pKa = 9.89VRR25 pKa = 11.84QGSPRR30 pKa = 11.84FAEE33 pKa = 4.29VRR35 pKa = 11.84RR36 pKa = 11.84GSPRR40 pKa = 11.84FAEE43 pKa = 4.49VRR45 pKa = 11.84QGSPRR50 pKa = 11.84FAKK53 pKa = 9.89VRR55 pKa = 11.84QGSPRR60 pKa = 11.84FAKK63 pKa = 9.89VRR65 pKa = 11.84QGSPRR70 pKa = 11.84FAKK73 pKa = 9.89VRR75 pKa = 11.84QGSPRR80 pKa = 11.84FAKK83 pKa = 9.89VRR85 pKa = 11.84QGSPRR90 pKa = 11.84FAKK93 pKa = 9.89VRR95 pKa = 11.84QGSPRR100 pKa = 11.84FAKK103 pKa = 9.89VRR105 pKa = 11.84QGSPRR110 pKa = 11.84FAKK113 pKa = 9.89VRR115 pKa = 11.84QGSPRR120 pKa = 11.84FAKK123 pKa = 9.89VRR125 pKa = 11.84QGSPRR130 pKa = 11.84FAKK133 pKa = 9.89VRR135 pKa = 11.84QGSPRR140 pKa = 11.84FAKK143 pKa = 9.89VRR145 pKa = 11.84QGSPRR150 pKa = 11.84FAKK153 pKa = 9.89VRR155 pKa = 11.84QGSPRR160 pKa = 11.84FAKK163 pKa = 9.89VRR165 pKa = 11.84QGSPRR170 pKa = 11.84FAKK173 pKa = 9.89VRR175 pKa = 11.84QGSPRR180 pKa = 11.84FAKK183 pKa = 9.89VRR185 pKa = 11.84QGSPRR190 pKa = 11.84FAKK193 pKa = 9.89VRR195 pKa = 11.84QGSPRR200 pKa = 11.84FAKK203 pKa = 9.89VRR205 pKa = 11.84QGSPRR210 pKa = 11.84FAKK213 pKa = 10.51

Molecular weight: 23.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30118

19

30137

9947364

20

7776

330.1

36.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.936 ± 0.018

1.928 ± 0.015

5.296 ± 0.012

6.405 ± 0.02

3.962 ± 0.013

5.692 ± 0.019

2.445 ± 0.008

5.278 ± 0.011

5.719 ± 0.018

9.161 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.007

4.655 ± 0.01

5.431 ± 0.018

4.44 ± 0.012

5.514 ± 0.014

8.381 ± 0.021

5.934 ± 0.02

6.41 ± 0.017

1.197 ± 0.006

2.952 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski