Amino acid dipepetide frequency for Daphnia pulex (Water flea)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.176AlaAla: 7.176 ± 0.054	1.111AlaCys: 1.111 ± 0.013	3.563AlaAsp: 3.563 ± 0.022	4.303AlaGlu: 4.303 ± 0.028	2.51AlaPhe: 2.51 ± 0.019	4.062AlaGly: 4.062 ± 0.027	1.397AlaHis: 1.397 ± 0.012	3.736AlaIle: 3.736 ± 0.022	3.729AlaLys: 3.729 ± 0.024	6.058AlaLeu: 6.058 ± 0.028	1.556AlaMet: 1.556 ± 0.013	3.002AlaAsn: 3.002 ± 0.018	3.736AlaPro: 3.736 ± 0.04	2.678AlaGln: 2.678 ± 0.022	3.28AlaArg: 3.28 ± 0.022	5.598AlaSer: 5.598 ± 0.027	4.367AlaThr: 4.367 ± 0.024	5.054AlaVal: 5.054 ± 0.027	0.67AlaTrp: 0.67 ± 0.009	1.775AlaTyr: 1.775 ± 0.019	0.001AlaXaa: 0.001 ± 0.0
Cys	1.076CysAla: 1.076 ± 0.013	0.54CysCys: 0.54 ± 0.009	1.083CysAsp: 1.083 ± 0.014	0.975CysGlu: 0.975 ± 0.012	0.796CysPhe: 0.796 ± 0.009	1.269CysGly: 1.269 ± 0.016	0.534CysHis: 0.534 ± 0.009	0.958CysIle: 0.958 ± 0.013	0.949CysLys: 0.949 ± 0.012	1.877CysLeu: 1.877 ± 0.014	0.381CysMet: 0.381 ± 0.007	0.846CysAsn: 0.846 ± 0.01	1.127CysPro: 1.127 ± 0.018	0.9CysGln: 0.9 ± 0.014	1.22CysArg: 1.22 ± 0.015	1.638CysSer: 1.638 ± 0.019	1.01CysThr: 1.01 ± 0.013	1.203CysVal: 1.203 ± 0.015	0.284CysTrp: 0.284 ± 0.007	0.62CysTyr: 0.62 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	3.325AspAla: 3.325 ± 0.021	1.062AspCys: 1.062 ± 0.013	3.666AspAsp: 3.666 ± 0.025	4.152AspGlu: 4.152 ± 0.026	2.316AspPhe: 2.316 ± 0.016	3.535AspGly: 3.535 ± 0.021	1.216AspHis: 1.216 ± 0.011	2.844AspIle: 2.844 ± 0.018	2.582AspLys: 2.582 ± 0.017	4.792AspLeu: 4.792 ± 0.023	1.111AspMet: 1.111 ± 0.009	2.195AspAsn: 2.195 ± 0.018	2.673AspPro: 2.673 ± 0.019	2.07AspGln: 2.07 ± 0.017	2.629AspArg: 2.629 ± 0.02	4.186AspSer: 4.186 ± 0.027	2.328AspThr: 2.328 ± 0.016	3.772AspVal: 3.772 ± 0.031	0.755AspTrp: 0.755 ± 0.009	1.755AspTyr: 1.755 ± 0.015	0.0AspXaa: 0.0 ± 0.0
Glu	4.404GluAla: 4.404 ± 0.028	1.092GluCys: 1.092 ± 0.017	3.902GluAsp: 3.902 ± 0.024	6.339GluGlu: 6.339 ± 0.05	2.244GluPhe: 2.244 ± 0.016	2.889GluGly: 2.889 ± 0.02	1.235GluHis: 1.235 ± 0.011	3.727GluIle: 3.727 ± 0.024	4.513GluLys: 4.513 ± 0.033	5.613GluLeu: 5.613 ± 0.03	1.703GluMet: 1.703 ± 0.014	3.317GluAsn: 3.317 ± 0.021	2.6GluPro: 2.6 ± 0.025	2.531GluGln: 2.531 ± 0.02	3.51GluArg: 3.51 ± 0.025	4.619GluSer: 4.619 ± 0.028	3.728GluThr: 3.728 ± 0.023	3.639GluVal: 3.639 ± 0.034	0.758GluTrp: 0.758 ± 0.009	1.692GluTyr: 1.692 ± 0.016	0.001GluXaa: 0.001 ± 0.0
Phe	2.397PheAla: 2.397 ± 0.019	0.867PheCys: 0.867 ± 0.01	2.22PheAsp: 2.22 ± 0.016	2.131PheGlu: 2.131 ± 0.017	1.843PhePhe: 1.843 ± 0.017	2.424PheGly: 2.424 ± 0.019	1.126PheHis: 1.126 ± 0.012	2.153PheIle: 2.153 ± 0.018	2.007PheLys: 2.007 ± 0.016	3.847PheLeu: 3.847 ± 0.021	0.868PheMet: 0.868 ± 0.011	1.885PheAsn: 1.885 ± 0.017	1.898PhePro: 1.898 ± 0.014	1.812PheGln: 1.812 ± 0.015	2.133PheArg: 2.133 ± 0.014	3.249PheSer: 3.249 ± 0.02	2.199PheThr: 2.199 ± 0.018	2.705PheVal: 2.705 ± 0.018	0.496PheTrp: 0.496 ± 0.008	1.358PheTyr: 1.358 ± 0.012	0.0PheXaa: 0.0 ± 0.0
Gly	3.464GlyAla: 3.464 ± 0.026	1.071GlyCys: 1.071 ± 0.012	2.907GlyAsp: 2.907 ± 0.025	3.093GlyGlu: 3.093 ± 0.021	2.349GlyPhe: 2.349 ± 0.02	4.736GlyGly: 4.736 ± 0.051	1.51GlyHis: 1.51 ± 0.014	3.021GlyIle: 3.021 ± 0.022	3.296GlyLys: 3.296 ± 0.022	4.773GlyLeu: 4.773 ± 0.029	1.266GlyMet: 1.266 ± 0.014	2.712GlyAsn: 2.712 ± 0.021	2.594GlyPro: 2.594 ± 0.034	2.416GlyGln: 2.416 ± 0.021	3.436GlyArg: 3.436 ± 0.024	4.982GlySer: 4.982 ± 0.036	3.098GlyThr: 3.098 ± 0.02	3.499GlyVal: 3.499 ± 0.023	0.782GlyTrp: 0.782 ± 0.01	1.919GlyTyr: 1.919 ± 0.021	0.001GlyXaa: 0.001 ± 0.0
His	1.285HisAla: 1.285 ± 0.012	0.549HisCys: 0.549 ± 0.009	1.045HisAsp: 1.045 ± 0.011	1.223HisGlu: 1.223 ± 0.012	1.182HisPhe: 1.182 ± 0.011	1.429HisGly: 1.429 ± 0.014	1.19HisHis: 1.19 ± 0.022	1.183HisIle: 1.183 ± 0.013	1.035HisLys: 1.035 ± 0.01	2.604HisLeu: 2.604 ± 0.018	0.499HisMet: 0.499 ± 0.008	0.984HisAsn: 0.984 ± 0.01	1.565HisPro: 1.565 ± 0.015	1.401HisGln: 1.401 ± 0.014	1.498HisArg: 1.498 ± 0.014	1.977HisSer: 1.977 ± 0.015	1.112HisThr: 1.112 ± 0.01	1.482HisVal: 1.482 ± 0.012	0.343HisTrp: 0.343 ± 0.007	0.863HisTyr: 0.863 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	3.471IleAla: 3.471 ± 0.02	1.107IleCys: 1.107 ± 0.011	3.011IleAsp: 3.011 ± 0.018	3.28IleGlu: 3.28 ± 0.02	2.221IlePhe: 2.221 ± 0.016	2.889IleGly: 2.889 ± 0.02	1.392IleHis: 1.392 ± 0.013	2.884IleIle: 2.884 ± 0.023	2.856IleLys: 2.856 ± 0.021	4.888IleLeu: 4.888 ± 0.028	1.078IleMet: 1.078 ± 0.01	2.463IleAsn: 2.463 ± 0.016	3.0IlePro: 3.0 ± 0.02	2.443IleGln: 2.443 ± 0.018	2.963IleArg: 2.963 ± 0.017	4.186IleSer: 4.186 ± 0.02	3.014IleThr: 3.014 ± 0.019	3.483IleVal: 3.483 ± 0.021	0.601IleTrp: 0.601 ± 0.007	1.547IleTyr: 1.547 ± 0.014	0.001IleXaa: 0.001 ± 0.0
Lys	3.659LysAla: 3.659 ± 0.025	1.057LysCys: 1.057 ± 0.013	2.842LysAsp: 2.842 ± 0.02	4.29LysGlu: 4.29 ± 0.034	2.117LysPhe: 2.117 ± 0.016	2.697LysGly: 2.697 ± 0.025	1.197LysHis: 1.197 ± 0.012	3.267LysIle: 3.267 ± 0.021	4.676LysLys: 4.676 ± 0.045	5.221LysLeu: 5.221 ± 0.027	1.563LysMet: 1.563 ± 0.016	2.795LysAsn: 2.795 ± 0.018	2.785LysPro: 2.785 ± 0.022	2.278LysGln: 2.278 ± 0.018	3.435LysArg: 3.435 ± 0.02	4.286LysSer: 4.286 ± 0.027	3.357LysThr: 3.357 ± 0.023	3.249LysVal: 3.249 ± 0.02	0.698LysTrp: 0.698 ± 0.009	1.726LysTyr: 1.726 ± 0.016	0.001LysXaa: 0.001 ± 0.0
Leu	6.603LeuAla: 6.603 ± 0.032	1.77LeuCys: 1.77 ± 0.016	4.792LeuAsp: 4.792 ± 0.027	5.828LeuGlu: 5.828 ± 0.032	3.492LeuPhe: 3.492 ± 0.023	4.527LeuGly: 4.527 ± 0.024	2.348LeuHis: 2.348 ± 0.018	4.711LeuIle: 4.711 ± 0.028	5.673LeuLys: 5.673 ± 0.031	9.398LeuLeu: 9.398 ± 0.049	2.186LeuMet: 2.186 ± 0.015	4.172LeuAsn: 4.172 ± 0.021	5.127LeuPro: 5.127 ± 0.028	4.249LeuGln: 4.249 ± 0.027	5.155LeuArg: 5.155 ± 0.025	7.274LeuSer: 7.274 ± 0.033	5.209LeuThr: 5.209 ± 0.025	5.725LeuVal: 5.725 ± 0.025	1.004LeuTrp: 1.004 ± 0.011	2.362LeuTyr: 2.362 ± 0.02	0.002LeuXaa: 0.002 ± 0.0
Met	2.089MetAla: 2.089 ± 0.016	0.363MetCys: 0.363 ± 0.006	1.403MetAsp: 1.403 ± 0.012	1.792MetGlu: 1.792 ± 0.015	0.78MetPhe: 0.78 ± 0.01	1.235MetGly: 1.235 ± 0.016	0.435MetHis: 0.435 ± 0.007	1.076MetIle: 1.076 ± 0.011	1.509MetLys: 1.509 ± 0.012	1.904MetLeu: 1.904 ± 0.016	0.657MetMet: 0.657 ± 0.01	1.041MetAsn: 1.041 ± 0.01	1.087MetPro: 1.087 ± 0.012	0.857MetGln: 0.857 ± 0.011	1.087MetArg: 1.087 ± 0.01	1.764MetSer: 1.764 ± 0.014	1.423MetThr: 1.423 ± 0.014	1.321MetVal: 1.321 ± 0.013	0.252MetTrp: 0.252 ± 0.005	0.534MetTyr: 0.534 ± 0.008	0.001MetXaa: 0.001 ± 0.0
Asn	2.861AsnAla: 2.861 ± 0.019	0.978AsnCys: 0.978 ± 0.013	2.327AsnAsp: 2.327 ± 0.016	2.856AsnGlu: 2.856 ± 0.017	1.982AsnPhe: 1.982 ± 0.014	3.002AsnGly: 3.002 ± 0.021	1.156AsnHis: 1.156 ± 0.011	2.481AsnIle: 2.481 ± 0.018	2.516AsnLys: 2.516 ± 0.016	4.276AsnLeu: 4.276 ± 0.024	0.992AsnMet: 0.992 ± 0.011	2.678AsnAsn: 2.678 ± 0.023	2.573AsnPro: 2.573 ± 0.018	2.178AsnGln: 2.178 ± 0.017	2.509AsnArg: 2.509 ± 0.017	3.806AsnSer: 3.806 ± 0.022	2.247AsnThr: 2.247 ± 0.016	3.037AsnVal: 3.037 ± 0.018	0.602AsnTrp: 0.602 ± 0.007	1.496AsnTyr: 1.496 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	4.303ProAla: 4.303 ± 0.036	0.84ProCys: 0.84 ± 0.015	2.639ProAsp: 2.639 ± 0.018	3.34ProGlu: 3.34 ± 0.021	2.036ProPhe: 2.036 ± 0.017	2.991ProGly: 2.991 ± 0.036	1.14ProHis: 1.14 ± 0.012	2.751ProIle: 2.751 ± 0.018	2.811ProLys: 2.811 ± 0.021	4.378ProLeu: 4.378 ± 0.025	1.054ProMet: 1.054 ± 0.016	2.328ProAsn: 2.328 ± 0.017	4.29ProPro: 4.29 ± 0.049	2.052ProGln: 2.052 ± 0.018	2.499ProArg: 2.499 ± 0.019	5.297ProSer: 5.297 ± 0.037	3.657ProThr: 3.657 ± 0.029	3.956ProVal: 3.956 ± 0.027	0.544ProTrp: 0.544 ± 0.008	1.398ProTyr: 1.398 ± 0.014	0.001ProXaa: 0.001 ± 0.0
Gln	2.667GlnAla: 2.667 ± 0.022	0.821GlnCys: 0.821 ± 0.012	1.856GlnAsp: 1.856 ± 0.013	2.693GlnGlu: 2.693 ± 0.022	1.597GlnPhe: 1.597 ± 0.015	1.933GlnGly: 1.933 ± 0.017	1.334GlnHis: 1.334 ± 0.014	2.221GlnIle: 2.221 ± 0.013	2.338GlnLys: 2.338 ± 0.018	4.669GlnLeu: 4.669 ± 0.028	1.136GlnMet: 1.136 ± 0.012	1.833GlnAsn: 1.833 ± 0.017	2.679GlnPro: 2.679 ± 0.026	4.016GlnGln: 4.016 ± 0.059	2.652GlnArg: 2.652 ± 0.017	3.364GlnSer: 3.364 ± 0.022	2.382GlnThr: 2.382 ± 0.017	2.524GlnVal: 2.524 ± 0.017	0.545GlnTrp: 0.545 ± 0.007	1.14GlnTyr: 1.14 ± 0.013	0.001GlnXaa: 0.001 ± 0.0
Arg	3.162ArgAla: 3.162 ± 0.021	1.082ArgCys: 1.082 ± 0.013	2.714ArgAsp: 2.714 ± 0.018	3.256ArgGlu: 3.256 ± 0.023	2.173ArgPhe: 2.173 ± 0.016	2.821ArgGly: 2.821 ± 0.023	1.549ArgHis: 1.549 ± 0.013	2.957ArgIle: 2.957 ± 0.018	3.558ArgLys: 3.558 ± 0.024	5.304ArgLeu: 5.304 ± 0.027	1.287ArgMet: 1.287 ± 0.01	2.71ArgAsn: 2.71 ± 0.015	2.991ArgPro: 2.991 ± 0.025	2.739ArgGln: 2.739 ± 0.021	4.282ArgArg: 4.282 ± 0.03	4.374ArgSer: 4.374 ± 0.029	2.803ArgThr: 2.803 ± 0.019	3.081ArgVal: 3.081 ± 0.019	0.7ArgTrp: 0.7 ± 0.009	1.593ArgTyr: 1.593 ± 0.014	0.0ArgXaa: 0.0 ± 0.0
Ser	5.704SerAla: 5.704 ± 0.031	1.583SerCys: 1.583 ± 0.017	4.24SerAsp: 4.24 ± 0.023	4.353SerGlu: 4.353 ± 0.029	3.326SerPhe: 3.326 ± 0.019	5.044SerGly: 5.044 ± 0.029	1.936SerHis: 1.936 ± 0.016	4.078SerIle: 4.078 ± 0.022	4.219SerLys: 4.219 ± 0.023	7.38SerLeu: 7.38 ± 0.028	1.669SerMet: 1.669 ± 0.013	3.866SerAsn: 3.866 ± 0.025	4.645SerPro: 4.645 ± 0.032	3.346SerGln: 3.346 ± 0.021	4.469SerArg: 4.469 ± 0.027	9.868SerSer: 9.868 ± 0.069	5.501SerThr: 5.501 ± 0.035	5.188SerVal: 5.188 ± 0.025	0.939SerTrp: 0.939 ± 0.011	2.451SerTyr: 2.451 ± 0.023	0.001SerXaa: 0.001 ± 0.0
Thr	4.356ThrAla: 4.356 ± 0.022	1.18ThrCys: 1.18 ± 0.016	2.879ThrAsp: 2.879 ± 0.017	3.59ThrGlu: 3.59 ± 0.024	2.27ThrPhe: 2.27 ± 0.016	3.43ThrGly: 3.43 ± 0.024	1.234ThrHis: 1.234 ± 0.013	3.094ThrIle: 3.094 ± 0.021	3.201ThrLys: 3.201 ± 0.025	5.101ThrLeu: 5.101 ± 0.023	1.142ThrMet: 1.142 ± 0.01	2.645ThrAsn: 2.645 ± 0.018	3.59ThrPro: 3.59 ± 0.024	2.1ThrGln: 2.1 ± 0.015	2.747ThrArg: 2.747 ± 0.018	5.267ThrSer: 5.267 ± 0.029	5.276ThrThr: 5.276 ± 0.071	3.938ThrVal: 3.938 ± 0.021	0.656ThrTrp: 0.656 ± 0.008	1.646ThrTyr: 1.646 ± 0.014	0.001ThrXaa: 0.001 ± 0.0
Val	4.851ValAla: 4.851 ± 0.026	1.305ValCys: 1.305 ± 0.015	3.703ValAsp: 3.703 ± 0.033	4.296ValGlu: 4.296 ± 0.03	2.51ValPhe: 2.51 ± 0.02	3.428ValGly: 3.428 ± 0.031	1.496ValHis: 1.496 ± 0.012	3.486ValIle: 3.486 ± 0.019	3.503ValLys: 3.503 ± 0.021	5.668ValLeu: 5.668 ± 0.031	1.471ValMet: 1.471 ± 0.014	2.988ValAsn: 2.988 ± 0.019	3.292ValPro: 3.292 ± 0.024	2.617ValGln: 2.617 ± 0.017	3.23ValArg: 3.23 ± 0.022	4.897ValSer: 4.897 ± 0.025	3.989ValThr: 3.989 ± 0.023	4.797ValVal: 4.797 ± 0.04	0.737ValTrp: 0.737 ± 0.009	1.839ValTyr: 1.839 ± 0.017	0.001ValXaa: 0.001 ± 0.0
Trp	0.695TrpAla: 0.695 ± 0.009	0.241TrpCys: 0.241 ± 0.006	0.625TrpAsp: 0.625 ± 0.01	0.679TrpGlu: 0.679 ± 0.01	0.478TrpPhe: 0.478 ± 0.007	0.58TrpGly: 0.58 ± 0.009	0.273TrpHis: 0.273 ± 0.006	0.786TrpIle: 0.786 ± 0.01	0.825TrpLys: 0.825 ± 0.01	1.185TrpLeu: 1.185 ± 0.012	0.358TrpMet: 0.358 ± 0.006	0.725TrpAsn: 0.725 ± 0.009	0.532TrpPro: 0.532 ± 0.008	0.468TrpGln: 0.468 ± 0.008	0.742TrpArg: 0.742 ± 0.01	0.864TrpSer: 0.864 ± 0.011	0.767TrpThr: 0.767 ± 0.009	0.615TrpVal: 0.615 ± 0.008	0.192TrpTrp: 0.192 ± 0.005	0.338TrpTyr: 0.338 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.814TyrAla: 1.814 ± 0.02	0.665TyrCys: 0.665 ± 0.009	1.546TyrAsp: 1.546 ± 0.015	1.586TyrGlu: 1.586 ± 0.014	1.397TyrPhe: 1.397 ± 0.013	2.0TyrGly: 2.0 ± 0.024	0.749TyrHis: 0.749 ± 0.01	1.363TyrIle: 1.363 ± 0.013	1.398TyrLys: 1.398 ± 0.015	2.669TyrLeu: 2.669 ± 0.018	0.634TyrMet: 0.634 ± 0.008	1.369TyrAsn: 1.369 ± 0.013	1.519TyrPro: 1.519 ± 0.017	1.249TyrGln: 1.249 ± 0.013	1.661TyrArg: 1.661 ± 0.016	2.311TyrSer: 2.311 ± 0.018	1.877TyrThr: 1.877 ± 0.028	1.835TyrVal: 1.835 ± 0.015	0.41TyrTrp: 0.41 ± 0.007	1.471TyrTyr: 1.471 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.001XaaSer: 0.001 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 30137 proteins (9947364 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---