Mycobacterium phage XFactor

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2FPV0|A0A0K2FPV0_9CAUD Uncharacterized protein OS=Mycobacterium phage XFactor OX=1698715 GN=XFACTOR_60 PE=4 SV=1
MM1 pKa = 7.36AHH3 pKa = 6.42WKK5 pKa = 9.59YY6 pKa = 8.91WWTMPLLIAAGIIGPGLAAPEE27 pKa = 4.19AKK29 pKa = 10.36ADD31 pKa = 3.49ITSDD35 pKa = 3.54AFVMALDD42 pKa = 4.35SEE44 pKa = 5.11GITYY48 pKa = 10.39SSKK51 pKa = 10.3PAVINAGKK59 pKa = 10.01AVCDD63 pKa = 3.93VLDD66 pKa = 3.65TGYY69 pKa = 9.51TMYY72 pKa = 10.19EE73 pKa = 3.7ASVFVYY79 pKa = 10.8NNSNLDD85 pKa = 3.99LYY87 pKa = 11.33DD88 pKa = 3.2SGYY91 pKa = 10.79FVGAATASFCPEE103 pKa = 4.1HH104 pKa = 7.29LSGTGWVV111 pKa = 3.57

Molecular weight: 11.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2FPX7|A0A0K2FPX7_9CAUD Uncharacterized protein OS=Mycobacterium phage XFactor OX=1698715 GN=XFACTOR_90 PE=4 SV=1
MM1 pKa = 7.8AGTAVLTPDD10 pKa = 5.37GIDD13 pKa = 3.45TLVTAAEE20 pKa = 4.08AASLCGVTTSTIYY33 pKa = 10.15VWVNRR38 pKa = 11.84GTLAPSGKK46 pKa = 9.45NRR48 pKa = 11.84LGHH51 pKa = 5.16NVYY54 pKa = 10.31RR55 pKa = 11.84VLDD58 pKa = 3.67VAKK61 pKa = 10.59AEE63 pKa = 4.07HH64 pKa = 6.08ATRR67 pKa = 11.84AKK69 pKa = 10.42ARR71 pKa = 11.84RR72 pKa = 11.84HH73 pKa = 4.63RR74 pKa = 3.93

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

17747

30

1161

186.8

20.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.554 ± 0.543

1.178 ± 0.163

6.756 ± 0.217

5.601 ± 0.284

3.06 ± 0.209

9.044 ± 0.618

2.445 ± 0.2

4.373 ± 0.187

3.206 ± 0.17

7.46 ± 0.209

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.131

3.347 ± 0.167

6.142 ± 0.216

3.403 ± 0.209

6.75 ± 0.378

5.747 ± 0.281

6.711 ± 0.248

7.1 ± 0.259

2.299 ± 0.136

2.547 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski