Amino acid dipepetide frequency for Mycobacterium phage XFactor

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.82AlaAla: 14.82 ± 1.91
1.014AlaCys: 1.014 ± 0.251
7.157AlaAsp: 7.157 ± 0.593
6.368AlaGlu: 6.368 ± 0.674
2.592AlaPhe: 2.592 ± 0.377
10.538AlaGly: 10.538 ± 1.297
2.93AlaHis: 2.93 ± 0.434
4.452AlaIle: 4.452 ± 0.593
3.606AlaLys: 3.606 ± 0.415
7.889AlaLeu: 7.889 ± 0.672
2.141AlaMet: 2.141 ± 0.364
2.254AlaAsn: 2.254 ± 0.402
5.184AlaPro: 5.184 ± 0.666
3.325AlaGln: 3.325 ± 0.453
8.002AlaArg: 8.002 ± 0.735
5.184AlaSer: 5.184 ± 0.575
6.311AlaThr: 6.311 ± 0.553
6.875AlaVal: 6.875 ± 0.586
2.818AlaTrp: 2.818 ± 0.441
2.085AlaTyr: 2.085 ± 0.291
0.0AlaXaa: 0.0 ± 0.0
Cys
1.296CysAla: 1.296 ± 0.311
0.056CysCys: 0.056 ± 0.047
1.578CysAsp: 1.578 ± 0.353
0.62CysGlu: 0.62 ± 0.193
0.225CysPhe: 0.225 ± 0.118
1.296CysGly: 1.296 ± 0.297
0.169CysHis: 0.169 ± 0.091
0.113CysIle: 0.113 ± 0.075
0.564CysLys: 0.564 ± 0.176
0.564CysLeu: 0.564 ± 0.206
0.282CysMet: 0.282 ± 0.125
0.507CysAsn: 0.507 ± 0.163
1.071CysPro: 1.071 ± 0.282
0.169CysGln: 0.169 ± 0.1
0.845CysArg: 0.845 ± 0.256
0.62CysSer: 0.62 ± 0.193
0.676CysThr: 0.676 ± 0.191
0.733CysVal: 0.733 ± 0.2
0.225CysTrp: 0.225 ± 0.101
0.169CysTyr: 0.169 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
7.213AspAla: 7.213 ± 0.648
0.958AspCys: 0.958 ± 0.235
4.677AspAsp: 4.677 ± 0.527
3.381AspGlu: 3.381 ± 0.416
2.198AspPhe: 2.198 ± 0.304
6.818AspGly: 6.818 ± 0.742
1.296AspHis: 1.296 ± 0.252
2.141AspIle: 2.141 ± 0.346
1.183AspLys: 1.183 ± 0.219
6.48AspLeu: 6.48 ± 0.502
1.183AspMet: 1.183 ± 0.285
1.578AspAsn: 1.578 ± 0.32
5.579AspPro: 5.579 ± 0.671
1.916AspGln: 1.916 ± 0.341
5.072AspArg: 5.072 ± 0.689
3.55AspSer: 3.55 ± 0.494
4.452AspThr: 4.452 ± 0.464
4.057AspVal: 4.057 ± 0.521
1.747AspTrp: 1.747 ± 0.297
2.085AspTyr: 2.085 ± 0.328
0.0AspXaa: 0.0 ± 0.0
Glu
6.311GluAla: 6.311 ± 0.65
0.789GluCys: 0.789 ± 0.225
3.043GluAsp: 3.043 ± 0.332
2.536GluGlu: 2.536 ± 0.459
2.141GluPhe: 2.141 ± 0.294
2.705GluGly: 2.705 ± 0.364
1.86GluHis: 1.86 ± 0.405
2.367GluIle: 2.367 ± 0.33
1.747GluLys: 1.747 ± 0.289
5.353GluLeu: 5.353 ± 0.588
1.409GluMet: 1.409 ± 0.297
1.972GluAsn: 1.972 ± 0.308
2.705GluPro: 2.705 ± 0.445
2.987GluGln: 2.987 ± 0.438
4.733GluArg: 4.733 ± 0.548
2.705GluSer: 2.705 ± 0.423
3.832GluThr: 3.832 ± 0.604
3.494GluVal: 3.494 ± 0.415
1.409GluTrp: 1.409 ± 0.261
1.916GluTyr: 1.916 ± 0.393
0.0GluXaa: 0.0 ± 0.0
Phe
3.268PheAla: 3.268 ± 0.453
0.225PheCys: 0.225 ± 0.116
2.705PheAsp: 2.705 ± 0.501
1.634PheGlu: 1.634 ± 0.305
0.845PhePhe: 0.845 ± 0.28
2.987PheGly: 2.987 ± 0.65
0.564PheHis: 0.564 ± 0.213
1.521PheIle: 1.521 ± 0.296
0.789PheLys: 0.789 ± 0.233
1.803PheLeu: 1.803 ± 0.286
0.789PheMet: 0.789 ± 0.23
1.24PheAsn: 1.24 ± 0.342
1.521PhePro: 1.521 ± 0.311
0.958PheGln: 0.958 ± 0.356
1.465PheArg: 1.465 ± 0.28
1.465PheSer: 1.465 ± 0.298
2.987PheThr: 2.987 ± 0.374
2.31PheVal: 2.31 ± 0.293
0.676PheTrp: 0.676 ± 0.209
0.845PheTyr: 0.845 ± 0.263
0.0PheXaa: 0.0 ± 0.0
Gly
9.185GlyAla: 9.185 ± 1.399
1.24GlyCys: 1.24 ± 0.296
5.973GlyAsp: 5.973 ± 0.651
4.17GlyGlu: 4.17 ± 0.511
2.818GlyPhe: 2.818 ± 0.467
11.439GlyGly: 11.439 ± 2.72
2.141GlyHis: 2.141 ± 0.3
4.226GlyIle: 4.226 ± 0.591
2.818GlyLys: 2.818 ± 0.425
6.086GlyLeu: 6.086 ± 0.556
2.479GlyMet: 2.479 ± 0.446
3.156GlyAsn: 3.156 ± 0.48
4.677GlyPro: 4.677 ± 0.594
2.367GlyGln: 2.367 ± 0.565
5.015GlyArg: 5.015 ± 0.61
5.804GlySer: 5.804 ± 0.75
6.142GlyThr: 6.142 ± 0.766
6.142GlyVal: 6.142 ± 0.557
2.254GlyTrp: 2.254 ± 0.357
2.31GlyTyr: 2.31 ± 0.405
0.0GlyXaa: 0.0 ± 0.0
His
2.029HisAla: 2.029 ± 0.364
0.394HisCys: 0.394 ± 0.157
1.296HisAsp: 1.296 ± 0.242
1.409HisGlu: 1.409 ± 0.279
0.564HisPhe: 0.564 ± 0.155
1.916HisGly: 1.916 ± 0.306
1.127HisHis: 1.127 ± 0.294
1.634HisIle: 1.634 ± 0.365
0.902HisLys: 0.902 ± 0.244
1.409HisLeu: 1.409 ± 0.26
0.507HisMet: 0.507 ± 0.157
0.902HisAsn: 0.902 ± 0.232
1.634HisPro: 1.634 ± 0.239
0.902HisGln: 0.902 ± 0.216
2.31HisArg: 2.31 ± 0.423
1.071HisSer: 1.071 ± 0.237
1.465HisThr: 1.465 ± 0.246
1.578HisVal: 1.578 ± 0.363
0.507HisTrp: 0.507 ± 0.156
0.902HisTyr: 0.902 ± 0.23
0.0HisXaa: 0.0 ± 0.0
Ile
5.297IleAla: 5.297 ± 0.578
0.507IleCys: 0.507 ± 0.169
3.888IleAsp: 3.888 ± 0.526
3.381IleGlu: 3.381 ± 0.425
0.845IlePhe: 0.845 ± 0.258
3.832IleGly: 3.832 ± 0.518
1.916IleHis: 1.916 ± 0.4
1.465IleIle: 1.465 ± 0.295
1.014IleLys: 1.014 ± 0.263
2.085IleLeu: 2.085 ± 0.369
0.282IleMet: 0.282 ± 0.122
1.972IleAsn: 1.972 ± 0.338
3.043IlePro: 3.043 ± 0.484
1.409IleGln: 1.409 ± 0.284
2.874IleArg: 2.874 ± 0.408
1.578IleSer: 1.578 ± 0.331
3.55IleThr: 3.55 ± 0.476
2.93IleVal: 2.93 ± 0.395
0.902IleTrp: 0.902 ± 0.196
0.902IleTyr: 0.902 ± 0.231
0.0IleXaa: 0.0 ± 0.0
Lys
3.494LysAla: 3.494 ± 0.486
0.507LysCys: 0.507 ± 0.16
1.86LysAsp: 1.86 ± 0.315
1.465LysGlu: 1.465 ± 0.266
1.071LysPhe: 1.071 ± 0.186
2.93LysGly: 2.93 ± 0.375
1.352LysHis: 1.352 ± 0.255
0.958LysIle: 0.958 ± 0.293
1.127LysLys: 1.127 ± 0.319
2.536LysLeu: 2.536 ± 0.483
0.733LysMet: 0.733 ± 0.152
0.845LysAsn: 0.845 ± 0.211
2.254LysPro: 2.254 ± 0.481
1.521LysGln: 1.521 ± 0.293
1.803LysArg: 1.803 ± 0.297
1.747LysSer: 1.747 ± 0.3
1.972LysThr: 1.972 ± 0.317
2.198LysVal: 2.198 ± 0.392
0.507LysTrp: 0.507 ± 0.143
1.183LysTyr: 1.183 ± 0.308
0.0LysXaa: 0.0 ± 0.0
Leu
7.607LeuAla: 7.607 ± 0.845
0.564LeuCys: 0.564 ± 0.189
4.903LeuAsp: 4.903 ± 0.616
4.114LeuGlu: 4.114 ± 0.499
2.93LeuPhe: 2.93 ± 0.402
5.635LeuGly: 5.635 ± 0.565
1.071LeuHis: 1.071 ± 0.237
4.001LeuIle: 4.001 ± 0.516
1.803LeuLys: 1.803 ± 0.29
4.79LeuLeu: 4.79 ± 0.496
1.521LeuMet: 1.521 ± 0.287
2.705LeuAsn: 2.705 ± 0.403
4.959LeuPro: 4.959 ± 0.641
2.761LeuGln: 2.761 ± 0.381
5.353LeuArg: 5.353 ± 0.649
5.86LeuSer: 5.86 ± 0.618
5.917LeuThr: 5.917 ± 0.574
4.79LeuVal: 4.79 ± 0.637
1.296LeuTrp: 1.296 ± 0.254
2.029LeuTyr: 2.029 ± 0.399
0.0LeuXaa: 0.0 ± 0.0
Met
2.648MetAla: 2.648 ± 0.383
0.113MetCys: 0.113 ± 0.083
1.578MetAsp: 1.578 ± 0.276
0.902MetGlu: 0.902 ± 0.186
0.564MetPhe: 0.564 ± 0.207
1.803MetGly: 1.803 ± 0.291
0.225MetHis: 0.225 ± 0.091
0.845MetIle: 0.845 ± 0.208
0.845MetLys: 0.845 ± 0.261
1.803MetLeu: 1.803 ± 0.248
0.733MetMet: 0.733 ± 0.261
1.014MetAsn: 1.014 ± 0.244
1.24MetPro: 1.24 ± 0.28
0.451MetGln: 0.451 ± 0.134
1.24MetArg: 1.24 ± 0.299
2.367MetSer: 2.367 ± 0.401
2.141MetThr: 2.141 ± 0.32
1.465MetVal: 1.465 ± 0.395
0.394MetTrp: 0.394 ± 0.15
0.394MetTyr: 0.394 ± 0.147
0.0MetXaa: 0.0 ± 0.0
Asn
3.494AsnAla: 3.494 ± 0.443
0.113AsnCys: 0.113 ± 0.074
2.141AsnAsp: 2.141 ± 0.35
1.521AsnGlu: 1.521 ± 0.301
0.789AsnPhe: 0.789 ± 0.338
3.719AsnGly: 3.719 ± 0.536
0.902AsnHis: 0.902 ± 0.197
1.465AsnIle: 1.465 ± 0.425
1.127AsnLys: 1.127 ± 0.266
2.31AsnLeu: 2.31 ± 0.333
0.451AsnMet: 0.451 ± 0.139
2.085AsnAsn: 2.085 ± 0.366
2.987AsnPro: 2.987 ± 0.419
1.183AsnGln: 1.183 ± 0.381
2.198AsnArg: 2.198 ± 0.475
1.465AsnSer: 1.465 ± 0.283
2.141AsnThr: 2.141 ± 0.296
1.972AsnVal: 1.972 ± 0.349
0.676AsnTrp: 0.676 ± 0.166
0.733AsnTyr: 0.733 ± 0.138
0.0AsnXaa: 0.0 ± 0.0
Pro
5.297ProAla: 5.297 ± 0.6
0.733ProCys: 0.733 ± 0.172
4.621ProAsp: 4.621 ± 0.614
4.226ProGlu: 4.226 ± 0.434
2.085ProPhe: 2.085 ± 0.409
6.875ProGly: 6.875 ± 0.633
1.747ProHis: 1.747 ± 0.267
1.916ProIle: 1.916 ± 0.317
2.254ProLys: 2.254 ± 0.507
4.395ProLeu: 4.395 ± 0.504
1.691ProMet: 1.691 ± 0.338
2.31ProAsn: 2.31 ± 0.31
4.057ProPro: 4.057 ± 0.638
2.254ProGln: 2.254 ± 0.317
3.099ProArg: 3.099 ± 0.514
3.043ProSer: 3.043 ± 0.411
3.212ProThr: 3.212 ± 0.474
5.128ProVal: 5.128 ± 0.573
1.014ProTrp: 1.014 ± 0.225
1.465ProTyr: 1.465 ± 0.286
0.0ProXaa: 0.0 ± 0.0
Gln
4.395GlnAla: 4.395 ± 0.575
0.507GlnCys: 0.507 ± 0.175
1.465GlnAsp: 1.465 ± 0.265
1.465GlnGlu: 1.465 ± 0.266
1.127GlnPhe: 1.127 ± 0.247
2.31GlnGly: 2.31 ± 0.438
0.733GlnHis: 0.733 ± 0.22
1.747GlnIle: 1.747 ± 0.261
1.183GlnLys: 1.183 ± 0.2
3.494GlnLeu: 3.494 ± 0.467
0.789GlnMet: 0.789 ± 0.24
0.62GlnAsn: 0.62 ± 0.195
2.198GlnPro: 2.198 ± 0.402
1.127GlnGln: 1.127 ± 0.244
2.874GlnArg: 2.874 ± 0.451
2.367GlnSer: 2.367 ± 0.379
1.803GlnThr: 1.803 ± 0.357
2.479GlnVal: 2.479 ± 0.379
0.564GlnTrp: 0.564 ± 0.148
0.789GlnTyr: 0.789 ± 0.254
0.0GlnXaa: 0.0 ± 0.0
Arg
6.086ArgAla: 6.086 ± 0.658
1.24ArgCys: 1.24 ± 0.356
4.452ArgAsp: 4.452 ± 0.519
4.564ArgGlu: 4.564 ± 0.614
2.423ArgPhe: 2.423 ± 0.438
4.114ArgGly: 4.114 ± 0.466
1.352ArgHis: 1.352 ± 0.318
3.832ArgIle: 3.832 ± 0.546
2.198ArgLys: 2.198 ± 0.386
5.128ArgLeu: 5.128 ± 0.62
2.367ArgMet: 2.367 ± 0.387
2.423ArgAsn: 2.423 ± 0.432
3.212ArgPro: 3.212 ± 0.408
2.085ArgGln: 2.085 ± 0.296
6.086ArgArg: 6.086 ± 0.921
3.606ArgSer: 3.606 ± 0.408
3.719ArgThr: 3.719 ± 0.467
4.903ArgVal: 4.903 ± 0.632
1.916ArgTrp: 1.916 ± 0.365
1.803ArgTyr: 1.803 ± 0.286
0.0ArgXaa: 0.0 ± 0.0
Ser
5.748SerAla: 5.748 ± 0.839
0.564SerCys: 0.564 ± 0.236
3.719SerAsp: 3.719 ± 0.488
3.099SerGlu: 3.099 ± 0.422
2.367SerPhe: 2.367 ± 0.422
6.368SerGly: 6.368 ± 0.756
1.183SerHis: 1.183 ± 0.282
2.648SerIle: 2.648 ± 0.371
2.648SerLys: 2.648 ± 0.474
4.17SerLeu: 4.17 ± 0.529
1.465SerMet: 1.465 ± 0.273
2.029SerAsn: 2.029 ± 0.342
3.325SerPro: 3.325 ± 0.449
1.747SerGln: 1.747 ± 0.278
2.367SerArg: 2.367 ± 0.362
3.663SerSer: 3.663 ± 0.584
3.212SerThr: 3.212 ± 0.468
4.339SerVal: 4.339 ± 0.474
1.521SerTrp: 1.521 ± 0.287
1.296SerTyr: 1.296 ± 0.198
0.0SerXaa: 0.0 ± 0.0
Thr
5.748ThrAla: 5.748 ± 0.519
0.733ThrCys: 0.733 ± 0.185
4.001ThrAsp: 4.001 ± 0.588
3.606ThrGlu: 3.606 ± 0.417
1.634ThrPhe: 1.634 ± 0.412
6.199ThrGly: 6.199 ± 0.601
1.634ThrHis: 1.634 ± 0.267
3.381ThrIle: 3.381 ± 0.42
2.141ThrLys: 2.141 ± 0.305
4.903ThrLeu: 4.903 ± 0.554
1.352ThrMet: 1.352 ± 0.28
2.423ThrAsn: 2.423 ± 0.364
5.579ThrPro: 5.579 ± 0.584
1.972ThrGln: 1.972 ± 0.321
3.945ThrArg: 3.945 ± 0.446
4.057ThrSer: 4.057 ± 0.34
4.959ThrThr: 4.959 ± 0.679
5.522ThrVal: 5.522 ± 0.582
1.183ThrTrp: 1.183 ± 0.268
2.141ThrTyr: 2.141 ± 0.39
0.0ThrXaa: 0.0 ± 0.0
Val
6.762ValAla: 6.762 ± 0.492
0.958ValCys: 0.958 ± 0.23
5.241ValAsp: 5.241 ± 0.532
4.564ValGlu: 4.564 ± 0.641
2.029ValPhe: 2.029 ± 0.356
5.86ValGly: 5.86 ± 0.705
1.24ValHis: 1.24 ± 0.289
2.93ValIle: 2.93 ± 0.427
2.198ValLys: 2.198 ± 0.321
5.41ValLeu: 5.41 ± 0.597
1.747ValMet: 1.747 ± 0.272
2.141ValAsn: 2.141 ± 0.382
3.606ValPro: 3.606 ± 0.431
2.93ValGln: 2.93 ± 0.334
4.339ValArg: 4.339 ± 0.589
5.015ValSer: 5.015 ± 0.604
4.903ValThr: 4.903 ± 0.479
5.917ValVal: 5.917 ± 0.681
1.972ValTrp: 1.972 ± 0.395
1.24ValTyr: 1.24 ± 0.253
0.0ValXaa: 0.0 ± 0.0
Trp
2.198TrpAla: 2.198 ± 0.346
0.225TrpCys: 0.225 ± 0.104
1.409TrpAsp: 1.409 ± 0.287
1.24TrpGlu: 1.24 ± 0.306
0.676TrpPhe: 0.676 ± 0.182
1.071TrpGly: 1.071 ± 0.31
0.733TrpHis: 0.733 ± 0.221
1.014TrpIle: 1.014 ± 0.217
1.071TrpLys: 1.071 ± 0.206
1.86TrpLeu: 1.86 ± 0.329
0.62TrpMet: 0.62 ± 0.213
0.564TrpAsn: 0.564 ± 0.206
1.24TrpPro: 1.24 ± 0.299
1.127TrpGln: 1.127 ± 0.269
1.972TrpArg: 1.972 ± 0.404
1.24TrpSer: 1.24 ± 0.335
1.578TrpThr: 1.578 ± 0.286
1.803TrpVal: 1.803 ± 0.439
1.014TrpTrp: 1.014 ± 0.218
0.338TrpTyr: 0.338 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.648TyrAla: 2.648 ± 0.408
0.338TyrCys: 0.338 ± 0.129
1.86TyrAsp: 1.86 ± 0.377
1.747TyrGlu: 1.747 ± 0.283
0.676TyrPhe: 0.676 ± 0.193
2.029TyrGly: 2.029 ± 0.369
0.282TyrHis: 0.282 ± 0.119
1.071TyrIle: 1.071 ± 0.219
0.845TyrLys: 0.845 ± 0.222
2.141TyrLeu: 2.141 ± 0.315
0.169TyrMet: 0.169 ± 0.098
0.733TyrAsn: 0.733 ± 0.201
1.352TyrPro: 1.352 ± 0.263
0.845TyrGln: 0.845 ± 0.237
1.916TyrArg: 1.916 ± 0.372
1.071TyrSer: 1.071 ± 0.229
2.141TyrThr: 2.141 ± 0.36
2.367TyrVal: 2.367 ± 0.283
0.394TyrTrp: 0.394 ± 0.144
0.845TyrTyr: 0.845 ± 0.193
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (17747 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski