Proteome-pI 2.0 - Proteome Isoelectric Point Database

Fusarium oxysporum (Fusarium vascular wilt)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium; Fusarium oxysporum species complex

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 18061 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A420R3A2|A0A420R3A2_FUSOX Uncharacterized protein OS=Fusarium oxysporum OX=5507 GN=BFJ70_g16382 PE=4 SV=1

MM1 pKa = 7.29LAVPPTLTTMQLALSLSDD19 pKa = 3.89PACPDD24 pKa = 3.6GPDD27 pKa = 3.67DD28 pKa = 4.12GANGVSITCPDD39 pKa = 3.48NSDD42 pKa = 4.94DD43 pKa = 5.55NDD45 pKa = 4.05ANDD48 pKa = 4.11YY49 pKa = 10.59KK50 pKa = 10.62ISSYY54 pKa = 10.78RR55 pKa = 11.84RR56 pKa = 11.84GHH58 pKa = 4.74TFEE61 pKa = 5.26VILL64 pKa = 4.66

Molecular weight: 6.8 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A2H3TAR6|A0A2H3TAR6_FUSOX Related to xyloglucan endo-transglycosylase-like protein OS=Fusarium oxysporum OX=5507 GN=BFJ69_g8673 PE=4 SV=1

MM1 pKa = 7.86PLTRR5 pKa = 11.84THH7 pKa = 6.63RR8 pKa = 11.84HH9 pKa = 4.04TAPRR13 pKa = 11.84RR14 pKa = 11.84SIFSTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84APAHH26 pKa = 4.84SHH28 pKa = 5.33RR29 pKa = 11.84HH30 pKa = 4.03TTTTTTTTTKK40 pKa = 9.5PRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84GMFGGGAGRR53 pKa = 11.84RR54 pKa = 11.84THH56 pKa = 6.46ATTTAPVHH64 pKa = 4.87HH65 pKa = 6.63HH66 pKa = 5.14QRR68 pKa = 11.84RR69 pKa = 11.84PSMKK73 pKa = 10.02DD74 pKa = 2.95KK75 pKa = 11.41VSGALLKK82 pKa = 11.04LKK84 pKa = 10.68GSLTRR89 pKa = 11.84RR90 pKa = 11.84PGVKK94 pKa = 9.89AAGTRR99 pKa = 11.84RR100 pKa = 11.84MRR102 pKa = 11.84GTDD105 pKa = 3.02GRR107 pKa = 11.84GARR110 pKa = 11.84HH111 pKa = 5.87HH112 pKa = 7.23RR113 pKa = 11.84YY114 pKa = 9.44

Molecular weight: 12.82 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
17790	271	18061	8091687	18	7605	448.0	49.72

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
8.331 ± 0.017	1.33 ± 0.008	5.819 ± 0.013	6.171 ± 0.018	3.825 ± 0.012	6.789 ± 0.018	2.388 ± 0.008	5.189 ± 0.014	4.988 ± 0.015	8.877 ± 0.021

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.235 ± 0.008	3.803 ± 0.009	5.824 ± 0.022	4.008 ± 0.014	5.821 ± 0.017	8.039 ± 0.019	6.023 ± 0.024	6.123 ± 0.014	1.584 ± 0.007	2.835 ± 0.009

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski