Aeromonas phage vB_AhyS-A18P4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6T5J6|A0A5J6T5J6_9CAUD Uncharacterized protein OS=Aeromonas phage vB_AhyS-A18P4 OX=2608321 PE=4 SV=1
MM1 pKa = 7.69ASNEE5 pKa = 4.07EE6 pKa = 4.18RR7 pKa = 11.84CPCCRR12 pKa = 11.84PIEE15 pKa = 4.41DD16 pKa = 3.67VADD19 pKa = 5.25AIAIVADD26 pKa = 4.47LGPTLMQMYY35 pKa = 9.75DD36 pKa = 3.47LRR38 pKa = 11.84IEE40 pKa = 4.45AVRR43 pKa = 11.84QMDD46 pKa = 3.06IHH48 pKa = 6.75LARR51 pKa = 11.84SGVSPDD57 pKa = 5.13DD58 pKa = 3.53MNHH61 pKa = 6.66PLNVALMQAQTEE73 pKa = 4.08IHH75 pKa = 6.51ALHH78 pKa = 6.43ILVRR82 pKa = 11.84ASGVMAGIPDD92 pKa = 4.27CADD95 pKa = 3.38VDD97 pKa = 3.81WDD99 pKa = 3.68DD100 pKa = 3.76AAAAFHH106 pKa = 7.13SDD108 pKa = 2.86TDD110 pKa = 3.97FTQGSTAAPVDD121 pKa = 3.94KK122 pKa = 10.42TKK124 pKa = 10.63HH125 pKa = 4.78

Molecular weight: 13.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6T285|A0A5J6T285_9CAUD Uncharacterized protein OS=Aeromonas phage vB_AhyS-A18P4 OX=2608321 PE=4 SV=1
MM1 pKa = 7.84PKK3 pKa = 8.56HH4 pKa = 5.35TTLQAITTAHH14 pKa = 6.45GVTVYY19 pKa = 10.88QLIQTYY25 pKa = 6.83GHH27 pKa = 6.49RR28 pKa = 11.84PEE30 pKa = 4.22TLRR33 pKa = 11.84RR34 pKa = 11.84WADD37 pKa = 3.59TQPQTLIALIAAMKK51 pKa = 9.9SGAVSVPLLASKK63 pKa = 11.05GPDD66 pKa = 2.79QVARR70 pKa = 11.84LRR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 8.97AAQARR79 pKa = 11.84YY80 pKa = 7.64YY81 pKa = 10.54QKK83 pKa = 10.92NKK85 pKa = 10.01KK86 pKa = 9.57KK87 pKa = 9.21RR88 pKa = 11.84TRR90 pKa = 11.84EE91 pKa = 3.62QRR93 pKa = 11.84QARR96 pKa = 11.84HH97 pKa = 4.29QARR100 pKa = 11.84AQRR103 pKa = 11.84LAQHH107 pKa = 6.78LADD110 pKa = 4.01VARR113 pKa = 11.84LLAWGVPAKK122 pKa = 10.02PWTARR127 pKa = 11.84KK128 pKa = 8.98VATVAGRR135 pKa = 11.84LRR137 pKa = 11.84DD138 pKa = 3.6AGGLVAASDD147 pKa = 3.8VRR149 pKa = 11.84RR150 pKa = 11.84VLDD153 pKa = 3.84GQGWRR158 pKa = 11.84CAYY161 pKa = 9.8CGHH164 pKa = 6.74PHH166 pKa = 6.39GVQVRR171 pKa = 11.84RR172 pKa = 11.84IDD174 pKa = 3.51YY175 pKa = 10.01GRR177 pKa = 11.84QPDD180 pKa = 4.07PEE182 pKa = 4.23NLIGLCTFHH191 pKa = 7.26AQDD194 pKa = 3.75RR195 pKa = 11.84GTDD198 pKa = 3.14HH199 pKa = 7.39DD200 pKa = 3.75HH201 pKa = 8.39DD202 pKa = 4.04YY203 pKa = 11.23RR204 pKa = 11.84QAQGIAAVTRR214 pKa = 11.84WDD216 pKa = 4.15GII218 pKa = 3.86

Molecular weight: 24.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

19051

39

1479

261.0

28.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.826 ± 0.556

1.092 ± 0.141

6.588 ± 0.233

5.9 ± 0.317

2.95 ± 0.136

7.616 ± 0.28

1.879 ± 0.174

4.971 ± 0.154

4.698 ± 0.266

8.341 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.152

3.475 ± 0.155

5.06 ± 0.322

3.9 ± 0.258

6.483 ± 0.268

4.96 ± 0.17

5.648 ± 0.226

6.939 ± 0.262

1.858 ± 0.13

3.071 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski