Gordonia phage Vendetta

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vendettavirus; Gordonia virus Vendetta

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160DFF5|A0A160DFF5_9CAUD Uncharacterized protein OS=Gordonia phage Vendetta OX=1838082 GN=6 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.13VDD4 pKa = 5.52CQFCWDD10 pKa = 3.66VAEE13 pKa = 4.47VLAEE17 pKa = 4.58AGDD20 pKa = 3.85STQAIEE26 pKa = 3.68YY27 pKa = 9.33DD28 pKa = 3.59QLHH31 pKa = 5.57EE32 pKa = 4.21HH33 pKa = 6.39AHH35 pKa = 6.24RR36 pKa = 11.84GAAVYY41 pKa = 10.6DD42 pKa = 4.04GGSLL46 pKa = 3.68

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160DD49|A0A160DD49_9CAUD Uncharacterized protein OS=Gordonia phage Vendetta OX=1838082 GN=62 PE=4 SV=1
MM1 pKa = 6.42TAKK4 pKa = 10.53GAGRR8 pKa = 11.84STRR11 pKa = 11.84RR12 pKa = 11.84WRR14 pKa = 11.84RR15 pKa = 11.84LKK17 pKa = 10.92AEE19 pKa = 4.16FRR21 pKa = 11.84RR22 pKa = 11.84LCADD26 pKa = 3.38RR27 pKa = 11.84RR28 pKa = 11.84APCWICGQPIDD39 pKa = 4.08YY40 pKa = 10.13RR41 pKa = 11.84LEE43 pKa = 4.45HH44 pKa = 6.38PHH46 pKa = 6.61PEE48 pKa = 3.64AWEE51 pKa = 4.18PDD53 pKa = 3.45HH54 pKa = 5.81YY55 pKa = 11.25HH56 pKa = 6.6PVAKK60 pKa = 10.35RR61 pKa = 11.84PDD63 pKa = 3.74LAEE66 pKa = 4.67DD67 pKa = 3.7PANLRR72 pKa = 11.84PSHH75 pKa = 6.16SSCNRR80 pKa = 11.84SRR82 pKa = 11.84GDD84 pKa = 3.41RR85 pKa = 11.84EE86 pKa = 4.17VVLGLGRR93 pKa = 11.84QSRR96 pKa = 11.84QWW98 pKa = 3.01

Molecular weight: 11.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

14347

26

1153

181.6

19.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.539 ± 0.49

0.767 ± 0.11

6.866 ± 0.232

6.538 ± 0.358

2.851 ± 0.15

9.64 ± 0.475

2.021 ± 0.162

3.422 ± 0.162

3.611 ± 0.259

7.333 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.111

2.656 ± 0.18

4.816 ± 0.23

3.067 ± 0.163

7.939 ± 0.359

5.228 ± 0.246

5.897 ± 0.228

8.099 ± 0.272

2.0 ± 0.117

2.488 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski