Amino acid dipepetide frequency for Gordonia phage Vendetta

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.66AlaAla: 16.66 ± 1.552
1.046AlaCys: 1.046 ± 0.297
7.877AlaAsp: 7.877 ± 0.752
8.434AlaGlu: 8.434 ± 0.941
3.416AlaPhe: 3.416 ± 0.54
14.429AlaGly: 14.429 ± 1.392
2.091AlaHis: 2.091 ± 0.393
4.67AlaIle: 4.67 ± 0.622
5.995AlaLys: 5.995 ± 0.794
10.247AlaLeu: 10.247 ± 0.862
2.719AlaMet: 2.719 ± 0.476
3.206AlaAsn: 3.206 ± 0.434
4.601AlaPro: 4.601 ± 0.664
3.555AlaGln: 3.555 ± 0.51
9.55AlaArg: 9.55 ± 0.884
5.995AlaSer: 5.995 ± 0.608
7.18AlaThr: 7.18 ± 0.738
8.434AlaVal: 8.434 ± 0.945
2.231AlaTrp: 2.231 ± 0.328
3.067AlaTyr: 3.067 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
0.767CysAla: 0.767 ± 0.234
0.0CysCys: 0.0 ± 0.0
0.418CysAsp: 0.418 ± 0.162
0.418CysGlu: 0.418 ± 0.176
0.139CysPhe: 0.139 ± 0.092
1.324CysGly: 1.324 ± 0.356
0.349CysHis: 0.349 ± 0.155
0.279CysIle: 0.279 ± 0.117
0.279CysLys: 0.279 ± 0.138
0.209CysLeu: 0.209 ± 0.119
0.07CysMet: 0.07 ± 0.078
0.349CysAsn: 0.349 ± 0.172
0.418CysPro: 0.418 ± 0.223
0.279CysGln: 0.279 ± 0.135
0.627CysArg: 0.627 ± 0.206
0.418CysSer: 0.418 ± 0.132
0.209CysThr: 0.209 ± 0.13
0.418CysVal: 0.418 ± 0.174
0.349CysTrp: 0.349 ± 0.165
0.349CysTyr: 0.349 ± 0.162
0.0CysXaa: 0.0 ± 0.0
Asp
8.434AspAla: 8.434 ± 0.86
1.046AspCys: 1.046 ± 0.228
4.949AspAsp: 4.949 ± 0.643
6.692AspGlu: 6.692 ± 0.92
1.673AspPhe: 1.673 ± 0.354
5.716AspGly: 5.716 ± 0.695
1.255AspHis: 1.255 ± 0.364
1.185AspIle: 1.185 ± 0.266
1.952AspLys: 1.952 ± 0.423
4.74AspLeu: 4.74 ± 0.756
1.185AspMet: 1.185 ± 0.29
1.185AspAsn: 1.185 ± 0.252
4.391AspPro: 4.391 ± 0.562
3.276AspGln: 3.276 ± 0.474
5.367AspArg: 5.367 ± 0.501
3.764AspSer: 3.764 ± 0.488
2.858AspThr: 2.858 ± 0.483
6.343AspVal: 6.343 ± 0.741
0.976AspTrp: 0.976 ± 0.279
1.673AspTyr: 1.673 ± 0.353
0.0AspXaa: 0.0 ± 0.0
Glu
8.992GluAla: 8.992 ± 0.855
0.488GluCys: 0.488 ± 0.207
3.834GluAsp: 3.834 ± 0.621
2.649GluGlu: 2.649 ± 0.494
2.509GluPhe: 2.509 ± 0.42
5.228GluGly: 5.228 ± 0.71
2.091GluHis: 2.091 ± 0.406
1.255GluIle: 1.255 ± 0.355
1.673GluLys: 1.673 ± 0.313
7.04GluLeu: 7.04 ± 0.803
1.324GluMet: 1.324 ± 0.34
1.324GluAsn: 1.324 ± 0.281
2.649GluPro: 2.649 ± 0.422
2.3GluGln: 2.3 ± 0.448
5.855GluArg: 5.855 ± 0.702
3.416GluSer: 3.416 ± 0.487
3.904GluThr: 3.904 ± 0.635
5.507GluVal: 5.507 ± 0.789
1.812GluTrp: 1.812 ± 0.307
1.534GluTyr: 1.534 ± 0.329
0.0GluXaa: 0.0 ± 0.0
Phe
3.973PheAla: 3.973 ± 0.54
0.209PheCys: 0.209 ± 0.135
1.394PheAsp: 1.394 ± 0.286
2.649PheGlu: 2.649 ± 0.389
0.488PhePhe: 0.488 ± 0.162
2.719PheGly: 2.719 ± 0.522
0.488PheHis: 0.488 ± 0.156
0.767PheIle: 0.767 ± 0.204
0.836PheLys: 0.836 ± 0.216
1.743PheLeu: 1.743 ± 0.381
0.976PheMet: 0.976 ± 0.219
0.488PheAsn: 0.488 ± 0.15
0.767PhePro: 0.767 ± 0.261
0.906PheGln: 0.906 ± 0.222
2.719PheArg: 2.719 ± 0.39
1.812PheSer: 1.812 ± 0.337
2.579PheThr: 2.579 ± 0.414
2.161PheVal: 2.161 ± 0.446
0.279PheTrp: 0.279 ± 0.136
0.558PheTyr: 0.558 ± 0.204
0.0PheXaa: 0.0 ± 0.0
Gly
8.853GlyAla: 8.853 ± 1.086
0.697GlyCys: 0.697 ± 0.26
7.249GlyAsp: 7.249 ± 0.589
6.622GlyGlu: 6.622 ± 0.582
3.137GlyPhe: 3.137 ± 0.505
11.432GlyGly: 11.432 ± 1.553
2.231GlyHis: 2.231 ± 0.381
3.206GlyIle: 3.206 ± 0.475
4.182GlyLys: 4.182 ± 0.479
6.204GlyLeu: 6.204 ± 0.731
3.206GlyMet: 3.206 ± 0.385
2.928GlyAsn: 2.928 ± 0.542
4.879GlyPro: 4.879 ± 0.719
3.206GlyGln: 3.206 ± 0.424
7.249GlyArg: 7.249 ± 0.496
5.089GlySer: 5.089 ± 0.555
4.67GlyThr: 4.67 ± 0.547
6.971GlyVal: 6.971 ± 0.937
2.231GlyTrp: 2.231 ± 0.394
2.161GlyTyr: 2.161 ± 0.467
0.0GlyXaa: 0.0 ± 0.0
His
2.649HisAla: 2.649 ± 0.522
0.139HisCys: 0.139 ± 0.108
1.673HisAsp: 1.673 ± 0.366
1.255HisGlu: 1.255 ± 0.319
0.418HisPhe: 0.418 ± 0.155
2.161HisGly: 2.161 ± 0.35
0.349HisHis: 0.349 ± 0.154
0.488HisIle: 0.488 ± 0.207
0.767HisLys: 0.767 ± 0.202
0.976HisLeu: 0.976 ± 0.257
0.349HisMet: 0.349 ± 0.139
0.558HisAsn: 0.558 ± 0.173
1.046HisPro: 1.046 ± 0.283
0.697HisGln: 0.697 ± 0.255
1.394HisArg: 1.394 ± 0.293
0.558HisSer: 0.558 ± 0.177
0.976HisThr: 0.976 ± 0.24
2.44HisVal: 2.44 ± 0.522
0.627HisTrp: 0.627 ± 0.23
0.697HisTyr: 0.697 ± 0.23
0.0HisXaa: 0.0 ± 0.0
Ile
5.576IleAla: 5.576 ± 0.62
0.209IleCys: 0.209 ± 0.123
2.579IleAsp: 2.579 ± 0.421
4.043IleGlu: 4.043 ± 0.604
0.558IlePhe: 0.558 ± 0.175
3.346IleGly: 3.346 ± 0.448
0.349IleHis: 0.349 ± 0.138
0.976IleIle: 0.976 ± 0.283
0.418IleLys: 0.418 ± 0.187
1.952IleLeu: 1.952 ± 0.426
0.697IleMet: 0.697 ± 0.226
0.209IleAsn: 0.209 ± 0.123
2.649IlePro: 2.649 ± 0.325
1.115IleGln: 1.115 ± 0.285
2.021IleArg: 2.021 ± 0.454
1.603IleSer: 1.603 ± 0.319
1.952IleThr: 1.952 ± 0.388
2.928IleVal: 2.928 ± 0.372
0.209IleTrp: 0.209 ± 0.109
0.836IleTyr: 0.836 ± 0.286
0.0IleXaa: 0.0 ± 0.0
Lys
5.019LysAla: 5.019 ± 0.709
0.349LysCys: 0.349 ± 0.161
2.231LysAsp: 2.231 ± 0.421
0.906LysGlu: 0.906 ± 0.237
0.976LysPhe: 0.976 ± 0.356
3.416LysGly: 3.416 ± 0.392
0.349LysHis: 0.349 ± 0.152
0.836LysIle: 0.836 ± 0.216
0.558LysLys: 0.558 ± 0.254
3.067LysLeu: 3.067 ± 0.396
0.767LysMet: 0.767 ± 0.22
0.906LysAsn: 0.906 ± 0.27
2.161LysPro: 2.161 ± 0.413
0.767LysGln: 0.767 ± 0.269
4.391LysArg: 4.391 ± 0.675
2.649LysSer: 2.649 ± 0.393
1.812LysThr: 1.812 ± 0.377
3.555LysVal: 3.555 ± 0.614
0.627LysTrp: 0.627 ± 0.243
0.767LysTyr: 0.767 ± 0.257
0.0LysXaa: 0.0 ± 0.0
Leu
10.386LeuAla: 10.386 ± 0.802
0.558LeuCys: 0.558 ± 0.173
6.343LeuAsp: 6.343 ± 0.562
3.416LeuGlu: 3.416 ± 0.471
1.812LeuPhe: 1.812 ± 0.318
6.622LeuGly: 6.622 ± 0.633
1.534LeuHis: 1.534 ± 0.321
2.649LeuIle: 2.649 ± 0.394
2.858LeuLys: 2.858 ± 0.471
3.973LeuLeu: 3.973 ± 0.631
1.673LeuMet: 1.673 ± 0.394
1.812LeuAsn: 1.812 ± 0.429
3.694LeuPro: 3.694 ± 0.569
1.534LeuGln: 1.534 ± 0.298
5.716LeuArg: 5.716 ± 0.578
4.81LeuSer: 4.81 ± 0.657
5.367LeuThr: 5.367 ± 0.629
4.67LeuVal: 4.67 ± 0.582
1.743LeuTrp: 1.743 ± 0.325
2.161LeuTyr: 2.161 ± 0.316
0.0LeuXaa: 0.0 ± 0.0
Met
3.137MetAla: 3.137 ± 0.545
0.0MetCys: 0.0 ± 0.0
1.324MetAsp: 1.324 ± 0.305
0.697MetGlu: 0.697 ± 0.238
0.627MetPhe: 0.627 ± 0.195
1.464MetGly: 1.464 ± 0.284
0.349MetHis: 0.349 ± 0.159
0.836MetIle: 0.836 ± 0.285
1.255MetLys: 1.255 ± 0.305
1.394MetLeu: 1.394 ± 0.294
0.349MetMet: 0.349 ± 0.118
1.115MetAsn: 1.115 ± 0.265
1.046MetPro: 1.046 ± 0.251
0.558MetGln: 0.558 ± 0.234
1.603MetArg: 1.603 ± 0.345
1.812MetSer: 1.812 ± 0.339
1.882MetThr: 1.882 ± 0.436
1.603MetVal: 1.603 ± 0.317
0.836MetTrp: 0.836 ± 0.236
0.349MetTyr: 0.349 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
2.928AsnAla: 2.928 ± 0.527
0.139AsnCys: 0.139 ± 0.087
1.952AsnAsp: 1.952 ± 0.331
1.673AsnGlu: 1.673 ± 0.313
1.046AsnPhe: 1.046 ± 0.311
3.555AsnGly: 3.555 ± 0.466
0.488AsnHis: 0.488 ± 0.189
0.976AsnIle: 0.976 ± 0.324
0.697AsnLys: 0.697 ± 0.232
1.743AsnLeu: 1.743 ± 0.38
0.349AsnMet: 0.349 ± 0.133
0.139AsnAsn: 0.139 ± 0.087
2.579AsnPro: 2.579 ± 0.39
0.836AsnGln: 0.836 ± 0.259
2.021AsnArg: 2.021 ± 0.388
0.976AsnSer: 0.976 ± 0.244
1.394AsnThr: 1.394 ± 0.305
2.231AsnVal: 2.231 ± 0.365
0.139AsnTrp: 0.139 ± 0.117
0.697AsnTyr: 0.697 ± 0.204
0.0AsnXaa: 0.0 ± 0.0
Pro
7.528ProAla: 7.528 ± 0.804
0.488ProCys: 0.488 ± 0.177
4.391ProAsp: 4.391 ± 0.591
3.485ProGlu: 3.485 ± 0.434
1.394ProPhe: 1.394 ± 0.268
4.391ProGly: 4.391 ± 0.56
1.255ProHis: 1.255 ± 0.303
2.3ProIle: 2.3 ± 0.502
1.673ProLys: 1.673 ± 0.399
2.509ProLeu: 2.509 ± 0.423
0.976ProMet: 0.976 ± 0.265
1.464ProAsn: 1.464 ± 0.433
1.464ProPro: 1.464 ± 0.384
1.394ProGln: 1.394 ± 0.3
2.579ProArg: 2.579 ± 0.483
1.812ProSer: 1.812 ± 0.337
2.509ProThr: 2.509 ± 0.362
4.322ProVal: 4.322 ± 0.65
1.046ProTrp: 1.046 ± 0.31
1.185ProTyr: 1.185 ± 0.264
0.0ProXaa: 0.0 ± 0.0
Gln
3.834GlnAla: 3.834 ± 0.468
0.07GlnCys: 0.07 ± 0.071
1.185GlnAsp: 1.185 ± 0.291
0.767GlnGlu: 0.767 ± 0.218
0.836GlnPhe: 0.836 ± 0.215
2.231GlnGly: 2.231 ± 0.303
0.488GlnHis: 0.488 ± 0.171
0.976GlnIle: 0.976 ± 0.231
0.906GlnLys: 0.906 ± 0.247
3.346GlnLeu: 3.346 ± 0.558
0.697GlnMet: 0.697 ± 0.241
1.115GlnAsn: 1.115 ± 0.236
2.021GlnPro: 2.021 ± 0.361
1.464GlnGln: 1.464 ± 0.424
2.997GlnArg: 2.997 ± 0.584
2.231GlnSer: 2.231 ± 0.384
1.882GlnThr: 1.882 ± 0.347
2.021GlnVal: 2.021 ± 0.397
0.627GlnTrp: 0.627 ± 0.168
0.976GlnTyr: 0.976 ± 0.259
0.0GlnXaa: 0.0 ± 0.0
Arg
10.177ArgAla: 10.177 ± 1.097
0.627ArgCys: 0.627 ± 0.227
4.949ArgAsp: 4.949 ± 0.652
5.786ArgGlu: 5.786 ± 0.837
2.579ArgPhe: 2.579 ± 0.369
6.692ArgGly: 6.692 ± 0.82
1.743ArgHis: 1.743 ± 0.373
3.694ArgIle: 3.694 ± 0.489
2.858ArgLys: 2.858 ± 0.476
4.949ArgLeu: 4.949 ± 0.606
1.812ArgMet: 1.812 ± 0.405
2.788ArgAsn: 2.788 ± 0.419
3.067ArgPro: 3.067 ± 0.475
1.673ArgGln: 1.673 ± 0.319
7.737ArgArg: 7.737 ± 0.867
2.928ArgSer: 2.928 ± 0.439
4.182ArgThr: 4.182 ± 0.51
7.04ArgVal: 7.04 ± 0.906
1.324ArgTrp: 1.324 ± 0.275
2.719ArgTyr: 2.719 ± 0.414
0.0ArgXaa: 0.0 ± 0.0
Ser
6.971SerAla: 6.971 ± 0.846
0.558SerCys: 0.558 ± 0.181
3.694SerAsp: 3.694 ± 0.522
3.067SerGlu: 3.067 ± 0.415
1.185SerPhe: 1.185 ± 0.246
5.576SerGly: 5.576 ± 0.735
0.906SerHis: 0.906 ± 0.254
2.091SerIle: 2.091 ± 0.299
1.464SerLys: 1.464 ± 0.281
3.764SerLeu: 3.764 ± 0.548
1.115SerMet: 1.115 ± 0.27
1.952SerAsn: 1.952 ± 0.302
1.743SerPro: 1.743 ± 0.332
1.812SerGln: 1.812 ± 0.308
3.834SerArg: 3.834 ± 0.567
3.067SerSer: 3.067 ± 0.584
3.206SerThr: 3.206 ± 0.414
4.252SerVal: 4.252 ± 0.573
1.185SerTrp: 1.185 ± 0.338
0.836SerTyr: 0.836 ± 0.216
0.0SerXaa: 0.0 ± 0.0
Thr
5.576ThrAla: 5.576 ± 0.59
0.279ThrCys: 0.279 ± 0.136
4.252ThrAsp: 4.252 ± 0.484
3.625ThrGlu: 3.625 ± 0.615
2.3ThrPhe: 2.3 ± 0.423
4.949ThrGly: 4.949 ± 0.706
0.906ThrHis: 0.906 ± 0.285
3.137ThrIle: 3.137 ± 0.455
2.161ThrLys: 2.161 ± 0.331
4.67ThrLeu: 4.67 ± 0.503
1.115ThrMet: 1.115 ± 0.272
1.603ThrAsn: 1.603 ± 0.385
4.182ThrPro: 4.182 ± 0.612
1.255ThrGln: 1.255 ± 0.287
3.625ThrArg: 3.625 ± 0.418
2.37ThrSer: 2.37 ± 0.412
4.322ThrThr: 4.322 ± 1.007
5.925ThrVal: 5.925 ± 0.692
1.185ThrTrp: 1.185 ± 0.302
1.534ThrTyr: 1.534 ± 0.367
0.0ThrXaa: 0.0 ± 0.0
Val
9.898ValAla: 9.898 ± 0.769
0.349ValCys: 0.349 ± 0.184
5.367ValAsp: 5.367 ± 0.568
6.343ValGlu: 6.343 ± 0.827
2.021ValPhe: 2.021 ± 0.383
5.995ValGly: 5.995 ± 0.659
2.091ValHis: 2.091 ± 0.362
2.788ValIle: 2.788 ± 0.55
4.043ValLys: 4.043 ± 0.69
6.831ValLeu: 6.831 ± 0.775
1.743ValMet: 1.743 ± 0.315
1.882ValAsn: 1.882 ± 0.39
3.346ValPro: 3.346 ± 0.423
2.44ValGln: 2.44 ± 0.27
5.089ValArg: 5.089 ± 0.566
4.601ValSer: 4.601 ± 0.58
5.716ValThr: 5.716 ± 0.651
7.249ValVal: 7.249 ± 0.715
1.464ValTrp: 1.464 ± 0.323
1.743ValTyr: 1.743 ± 0.347
0.0ValXaa: 0.0 ± 0.0
Trp
1.812TrpAla: 1.812 ± 0.363
0.139TrpCys: 0.139 ± 0.088
1.464TrpAsp: 1.464 ± 0.288
1.185TrpGlu: 1.185 ± 0.352
0.488TrpPhe: 0.488 ± 0.156
1.324TrpGly: 1.324 ± 0.272
0.488TrpHis: 0.488 ± 0.169
0.627TrpIle: 0.627 ± 0.204
0.906TrpLys: 0.906 ± 0.261
2.161TrpLeu: 2.161 ± 0.333
0.418TrpMet: 0.418 ± 0.162
0.627TrpAsn: 0.627 ± 0.196
0.767TrpPro: 0.767 ± 0.251
0.836TrpGln: 0.836 ± 0.227
1.882TrpArg: 1.882 ± 0.359
1.394TrpSer: 1.394 ± 0.29
1.603TrpThr: 1.603 ± 0.316
0.836TrpVal: 0.836 ± 0.243
0.558TrpTrp: 0.558 ± 0.173
0.488TrpTyr: 0.488 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.231TyrAla: 2.231 ± 0.443
0.279TyrCys: 0.279 ± 0.124
1.534TyrAsp: 1.534 ± 0.338
1.673TyrGlu: 1.673 ± 0.281
0.906TyrPhe: 0.906 ± 0.257
3.834TyrGly: 3.834 ± 0.484
0.418TyrHis: 0.418 ± 0.158
0.488TyrIle: 0.488 ± 0.157
0.627TyrLys: 0.627 ± 0.215
1.812TyrLeu: 1.812 ± 0.331
0.627TyrMet: 0.627 ± 0.195
0.906TyrAsn: 0.906 ± 0.217
0.697TyrPro: 0.697 ± 0.225
0.767TyrGln: 0.767 ± 0.193
3.137TyrArg: 3.137 ± 0.515
0.976TyrSer: 0.976 ± 0.279
0.767TyrThr: 0.767 ± 0.224
2.091TyrVal: 2.091 ± 0.399
0.558TyrTrp: 0.558 ± 0.158
0.558TyrTyr: 0.558 ± 0.228
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (14347 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski