Sus scrofa papillomavirus 2
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A223FQV1|A0A223FQV1_9PAPI Regulatory protein E2 OS=Sus scrofa papillomavirus 2 OX=2025338 GN=E2 PE=3 SV=1
MM1 pKa = 7.11 MGVEE5 pKa = 4.0 PTLRR9 pKa = 11.84 DD10 pKa = 3.5 IVLMDD15 pKa = 4.54 LPCPLHH21 pKa = 6.48 LGCEE25 pKa = 4.04 EE26 pKa = 4.1 RR27 pKa = 11.84 LEE29 pKa = 5.23 DD30 pKa = 4.51 MMLPEE35 pKa = 5.74 DD36 pKa = 4.95 DD37 pKa = 4.54 EE38 pKa = 6.28 ASEE41 pKa = 4.41 APYY44 pKa = 10.15 KK45 pKa = 11.06 VSVLCGLCRR54 pKa = 11.84 KK55 pKa = 8.76 PLKK58 pKa = 10.57 FLVICSGEE66 pKa = 3.99 NIQRR70 pKa = 11.84 LEE72 pKa = 4.0 DD73 pKa = 3.07 VAARR77 pKa = 11.84 VKK79 pKa = 9.92 YY80 pKa = 10.63 LCRR83 pKa = 11.84 CCSARR88 pKa = 11.84 FDD90 pKa = 3.59
Molecular weight: 10.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.639
IPC2_protein 4.838
IPC_protein 4.685
Toseland 4.533
ProMoST 4.66
Dawson 4.622
Bjellqvist 4.813
Wikipedia 4.495
Rodwell 4.52
Grimsley 4.444
Solomon 4.622
Lehninger 4.571
Nozaki 4.736
DTASelect 4.876
Thurlkill 4.533
EMBOSS 4.507
Sillero 4.8
Patrickios 3.325
IPC_peptide 4.622
IPC2_peptide 4.787
IPC2.peptide.svr19 4.778
Protein with the highest isoelectric point:
>tr|A0A223FQV2|A0A223FQV2_9PAPI Major capsid protein L1 OS=Sus scrofa papillomavirus 2 OX=2025338 GN=L1 PE=3 SV=1
MM1 pKa = 7.43 EE2 pKa = 6.17 RR3 pKa = 11.84 ICHH6 pKa = 6.31 RR7 pKa = 11.84 LNVVQEE13 pKa = 4.3 EE14 pKa = 4.35 LLSLYY19 pKa = 7.87 EE20 pKa = 4.02 TAQTSLQSQIRR31 pKa = 11.84 HH32 pKa = 5.36 WDD34 pKa = 3.5 LVRR37 pKa = 11.84 KK38 pKa = 9.71 EE39 pKa = 4.35 MIFFYY44 pKa = 10.37 AARR47 pKa = 11.84 QSGVDD52 pKa = 3.3 SIGFQKK58 pKa = 10.43 VPPLQVSQAKK68 pKa = 9.82 AKK70 pKa = 10.07 EE71 pKa = 4.19 AIEE74 pKa = 4.0 MQMILQSLGEE84 pKa = 4.02 SDD86 pKa = 4.37 FAQEE90 pKa = 3.72 QWTLSDD96 pKa = 3.3 TSRR99 pKa = 11.84 EE100 pKa = 3.9 LWLCPPQGCFKK111 pKa = 10.76 KK112 pKa = 9.92 HH113 pKa = 5.46 GSSVDD118 pKa = 2.87 VWYY121 pKa = 10.33 DD122 pKa = 3.74 EE123 pKa = 4.31 NPEE126 pKa = 3.85 NANRR130 pKa = 11.84 YY131 pKa = 8.1 VSWGSIYY138 pKa = 10.67 HH139 pKa = 6.86 LDD141 pKa = 3.75 GNEE144 pKa = 3.61 RR145 pKa = 11.84 WRR147 pKa = 11.84 KK148 pKa = 7.47 TEE150 pKa = 3.79 GLCDD154 pKa = 3.58 HH155 pKa = 7.01 TGLYY159 pKa = 10.17 YY160 pKa = 10.47 VDD162 pKa = 3.25 EE163 pKa = 4.6 SGYY166 pKa = 9.6 RR167 pKa = 11.84 RR168 pKa = 11.84 YY169 pKa = 10.25 YY170 pKa = 10.3 IKK172 pKa = 10.71 FSVDD176 pKa = 2.64 AKK178 pKa = 10.31 RR179 pKa = 11.84 YY180 pKa = 8.49 SATGQWEE187 pKa = 4.64 VKK189 pKa = 9.94 FKK191 pKa = 11.2 NQIFSPILPVTSTVPATQCGGPAPEE216 pKa = 4.17 SCGRR220 pKa = 11.84 QTPEE224 pKa = 3.61 QQRR227 pKa = 11.84 IASPPGGHH235 pKa = 6.07 GVSPPASRR243 pKa = 11.84 CRR245 pKa = 11.84 GASPGTDD252 pKa = 2.64 RR253 pKa = 11.84 ADD255 pKa = 3.54 TPPPKK260 pKa = 9.89 RR261 pKa = 11.84 PRR263 pKa = 11.84 DD264 pKa = 3.65 TRR266 pKa = 11.84 PTEE269 pKa = 3.76 TSKK272 pKa = 10.73 GRR274 pKa = 11.84 RR275 pKa = 11.84 RR276 pKa = 11.84 RR277 pKa = 11.84 SIRR280 pKa = 11.84 PGLGPRR286 pKa = 11.84 TSIVATGPVWVPPIIRR302 pKa = 11.84 PPRR305 pKa = 11.84 FPGSGSPWAARR316 pKa = 11.84 WYY318 pKa = 10.75 AKK320 pKa = 10.45 FEE322 pKa = 3.84 EE323 pKa = 4.67 RR324 pKa = 11.84 QRR326 pKa = 11.84 QQQQQQQQQQQAPIRR341 pKa = 11.84 FPGPADD347 pKa = 3.46 TGDD350 pKa = 3.64 SSGSRR355 pKa = 11.84 VSRR358 pKa = 11.84 DD359 pKa = 3.35 PGHH362 pKa = 6.6 SSRR365 pKa = 11.84 DD366 pKa = 3.4 PSDD369 pKa = 3.9 PGDD372 pKa = 3.53 TRR374 pKa = 11.84 EE375 pKa = 4.86 PGDD378 pKa = 3.61 PCDD381 pKa = 4.01 PSEE384 pKa = 4.64 EE385 pKa = 4.5 GPATEE390 pKa = 4.84 SDD392 pKa = 3.75 SGSDD396 pKa = 3.04 SDD398 pKa = 4.05 PNTRR402 pKa = 11.84 RR403 pKa = 11.84 EE404 pKa = 4.28 SPPGPWDD411 pKa = 3.49 TRR413 pKa = 11.84 PTGPQPPRR421 pKa = 11.84 TPEE424 pKa = 3.48 EE425 pKa = 3.75 AAAAAAATVTPPGAKK440 pKa = 9.03 EE441 pKa = 3.24 KK442 pKa = 10.42 AYY444 pKa = 10.8 RR445 pKa = 11.84 EE446 pKa = 4.12 GRR448 pKa = 11.84 GLRR451 pKa = 11.84 RR452 pKa = 11.84 QRR454 pKa = 11.84 PPTPPEE460 pKa = 3.98 CYY462 pKa = 9.4 RR463 pKa = 11.84 RR464 pKa = 11.84 VPFTRR469 pKa = 11.84 QQSTKK474 pKa = 10.41 SRR476 pKa = 11.84 NTTLVSPKK484 pKa = 8.64 QTPVKK489 pKa = 10.11 QGPSSPLAASTPAAATRR506 pKa = 11.84 SRR508 pKa = 11.84 TTRR511 pKa = 11.84 RR512 pKa = 11.84 NRR514 pKa = 11.84 SRR516 pKa = 11.84 SPTNPVASQPVGIAGRR532 pKa = 11.84 TRR534 pKa = 11.84 RR535 pKa = 11.84 SRR537 pKa = 11.84 YY538 pKa = 8.21 PVEE541 pKa = 4.27 EE542 pKa = 4.34 EE543 pKa = 4.34 SCGGDD548 pKa = 3.13 PGGTLQSLRR557 pKa = 11.84 DD558 pKa = 3.58 PDD560 pKa = 3.55 NQRR563 pKa = 11.84 EE564 pKa = 4.05 QRR566 pKa = 11.84 PGQSLPVLGSPAVVLLKK583 pKa = 11.04 GGTNQLKK590 pKa = 8.58 CCRR593 pKa = 11.84 YY594 pKa = 9.45 RR595 pKa = 11.84 FRR597 pKa = 11.84 TRR599 pKa = 11.84 HH600 pKa = 3.5 QHH602 pKa = 6.71 LFLHH606 pKa = 7.08 CSSTWYY612 pKa = 9.56 WSDD615 pKa = 2.8 GNRR618 pKa = 11.84 IGRR621 pKa = 11.84 SRR623 pKa = 11.84 MFLTFRR629 pKa = 11.84 DD630 pKa = 3.97 PEE632 pKa = 4.05 QRR634 pKa = 11.84 DD635 pKa = 3.46 LFLQTVSIPRR645 pKa = 11.84 DD646 pKa = 3.19 VDD648 pKa = 3.31 IVTGTLFSLL657 pKa = 4.44
Molecular weight: 73.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.978
IPC2_protein 8.96
IPC_protein 9.048
Toseland 9.531
ProMoST 9.414
Dawson 9.838
Bjellqvist 9.663
Wikipedia 10.058
Rodwell 9.97
Grimsley 9.926
Solomon 9.882
Lehninger 9.838
Nozaki 9.765
DTASelect 9.589
Thurlkill 9.692
EMBOSS 9.984
Sillero 9.823
Patrickios 5.232
IPC_peptide 9.867
IPC2_peptide 8.726
IPC2.peptide.svr19 7.84
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2534
90
657
422.3
47.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.959 ± 0.3
2.407 ± 0.631
6.117 ± 0.403
5.722 ± 0.356
3.867 ± 0.511
7.103 ± 0.606
1.697 ± 0.127
4.301 ± 0.545
4.538 ± 0.891
8.09 ± 0.757
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.618 ± 0.435
3.473 ± 0.897
7.419 ± 1.133
4.657 ± 0.613
7.301 ± 1.054
8.287 ± 0.889
7.064 ± 0.765
5.998 ± 0.425
1.342 ± 0.337
3.039 ± 0.482
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here