Klebsiella phage AmPh_EK29

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Marfavirus; unclassified Marfavirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 287 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8PLW5|A0A5P8PLW5_9CAUD Uncharacterized protein OS=Klebsiella phage AmPh_EK29 OX=2653641 GN=AmPhEK29_0083 PE=4 SV=1
MM1 pKa = 7.21FRR3 pKa = 11.84LIDD6 pKa = 4.02EE7 pKa = 4.6YY8 pKa = 11.69VGAHH12 pKa = 6.22YY13 pKa = 10.24AYY15 pKa = 9.01TWLADD20 pKa = 3.94GGWDD24 pKa = 4.81CDD26 pKa = 5.45DD27 pKa = 4.95EE28 pKa = 5.93GLDD31 pKa = 5.6HH32 pKa = 7.25ANAWNALKK40 pKa = 10.84DD41 pKa = 4.52FINGLL46 pKa = 3.72

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8PK13|A0A5P8PK13_9CAUD Endoribonuclease OS=Klebsiella phage AmPh_EK29 OX=2653641 GN=AmPhEK29_0028 PE=4 SV=1
MM1 pKa = 7.54TIDD4 pKa = 3.07TRR6 pKa = 11.84NYY8 pKa = 5.53MTRR11 pKa = 11.84GDD13 pKa = 3.88LTKK16 pKa = 10.87AGAVTVAHH24 pKa = 6.38VKK26 pKa = 10.34NGHH29 pKa = 4.72TVGFVMPEE37 pKa = 3.72VLAQPGFYY45 pKa = 10.62FMVKK49 pKa = 10.0GAGEE53 pKa = 4.01HH54 pKa = 5.86RR55 pKa = 11.84AVGARR60 pKa = 11.84FFVGNQRR67 pKa = 11.84SAQGFNATLSHH78 pKa = 6.24IRR80 pKa = 11.84QGRR83 pKa = 11.84SQLARR88 pKa = 11.84TMASNNIVYY97 pKa = 9.64EE98 pKa = 4.04VLYY101 pKa = 10.69LPQSKK106 pKa = 9.18MKK108 pKa = 10.23PLTTGFKK115 pKa = 10.26KK116 pKa = 10.76GQLALAFTRR125 pKa = 11.84QHH127 pKa = 5.92SSEE130 pKa = 4.07MQTLSEE136 pKa = 4.67LNRR139 pKa = 11.84ILNDD143 pKa = 3.0NFKK146 pKa = 10.97FILQSYY152 pKa = 8.66

Molecular weight: 16.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

287

0

287

53265

30

1435

185.6

20.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.343 ± 0.199

1.134 ± 0.065

6.209 ± 0.098

7.172 ± 0.186

4.286 ± 0.112

6.289 ± 0.235

1.847 ± 0.082

6.95 ± 0.13

7.29 ± 0.181

7.515 ± 0.142

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.863 ± 0.084

5.135 ± 0.162

3.423 ± 0.106

3.454 ± 0.093

4.583 ± 0.093

6.381 ± 0.141

5.711 ± 0.198

6.828 ± 0.128

1.444 ± 0.063

4.143 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski