Amino acid dipepetide frequency for Klebsiella phage AmPh_EK29

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.914AlaAla: 5.914 ± 0.353
0.469AlaCys: 0.469 ± 0.11
4.487AlaAsp: 4.487 ± 0.32
5.445AlaGlu: 5.445 ± 0.388
2.61AlaPhe: 2.61 ± 0.238
4.431AlaGly: 4.431 ± 0.315
1.295AlaHis: 1.295 ± 0.143
5.088AlaIle: 5.088 ± 0.275
5.501AlaLys: 5.501 ± 0.36
5.951AlaLeu: 5.951 ± 0.357
1.765AlaMet: 1.765 ± 0.146
3.774AlaAsn: 3.774 ± 0.345
2.854AlaPro: 2.854 ± 0.243
2.854AlaGln: 2.854 ± 0.255
3.417AlaArg: 3.417 ± 0.269
4.6AlaSer: 4.6 ± 0.3
4.055AlaThr: 4.055 ± 0.426
5.05AlaVal: 5.05 ± 0.33
1.126AlaTrp: 1.126 ± 0.17
2.741AlaTyr: 2.741 ± 0.201
0.0AlaXaa: 0.0 ± 0.0
Cys
0.882CysAla: 0.882 ± 0.111
0.225CysCys: 0.225 ± 0.07
0.676CysAsp: 0.676 ± 0.124
0.807CysGlu: 0.807 ± 0.115
0.451CysPhe: 0.451 ± 0.09
0.789CysGly: 0.789 ± 0.136
0.282CysHis: 0.282 ± 0.069
0.807CysIle: 0.807 ± 0.139
0.601CysLys: 0.601 ± 0.111
0.676CysLeu: 0.676 ± 0.112
0.469CysMet: 0.469 ± 0.084
0.563CysAsn: 0.563 ± 0.103
0.544CysPro: 0.544 ± 0.113
0.3CysGln: 0.3 ± 0.07
0.507CysArg: 0.507 ± 0.095
0.882CysSer: 0.882 ± 0.135
0.544CysThr: 0.544 ± 0.108
0.62CysVal: 0.62 ± 0.117
0.131CysTrp: 0.131 ± 0.051
0.582CysTyr: 0.582 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
4.281AspAla: 4.281 ± 0.307
0.732AspCys: 0.732 ± 0.119
4.018AspAsp: 4.018 ± 0.347
4.75AspGlu: 4.75 ± 0.305
3.004AspPhe: 3.004 ± 0.211
4.525AspGly: 4.525 ± 0.298
1.014AspHis: 1.014 ± 0.136
4.637AspIle: 4.637 ± 0.274
4.168AspLys: 4.168 ± 0.314
4.656AspLeu: 4.656 ± 0.292
1.746AspMet: 1.746 ± 0.187
2.966AspAsn: 2.966 ± 0.229
2.234AspPro: 2.234 ± 0.251
1.746AspGln: 1.746 ± 0.175
2.234AspArg: 2.234 ± 0.195
4.018AspSer: 4.018 ± 0.244
3.286AspThr: 3.286 ± 0.226
4.112AspVal: 4.112 ± 0.252
1.126AspTrp: 1.126 ± 0.133
2.835AspTyr: 2.835 ± 0.295
0.0AspXaa: 0.0 ± 0.0
Glu
6.365GluAla: 6.365 ± 0.385
0.882GluCys: 0.882 ± 0.129
3.792GluAsp: 3.792 ± 0.295
5.088GluGlu: 5.088 ± 0.369
3.379GluPhe: 3.379 ± 0.235
4.205GluGly: 4.205 ± 0.286
1.389GluHis: 1.389 ± 0.179
5.426GluIle: 5.426 ± 0.337
4.337GluLys: 4.337 ± 0.364
7.097GluLeu: 7.097 ± 0.347
2.215GluMet: 2.215 ± 0.214
3.192GluAsn: 3.192 ± 0.257
1.727GluPro: 1.727 ± 0.195
2.591GluGln: 2.591 ± 0.23
3.173GluArg: 3.173 ± 0.242
3.511GluSer: 3.511 ± 0.272
3.98GluThr: 3.98 ± 0.27
4.975GluVal: 4.975 ± 0.323
0.976GluTrp: 0.976 ± 0.114
3.417GluTyr: 3.417 ± 0.295
0.0GluXaa: 0.0 ± 0.0
Phe
2.704PheAla: 2.704 ± 0.219
0.375PheCys: 0.375 ± 0.094
2.704PheAsp: 2.704 ± 0.197
3.98PheGlu: 3.98 ± 0.32
1.427PhePhe: 1.427 ± 0.161
2.572PheGly: 2.572 ± 0.198
0.676PheHis: 0.676 ± 0.124
2.891PheIle: 2.891 ± 0.226
3.736PheLys: 3.736 ± 0.287
2.779PheLeu: 2.779 ± 0.213
1.577PheMet: 1.577 ± 0.175
2.704PheAsn: 2.704 ± 0.211
1.145PhePro: 1.145 ± 0.13
1.314PheGln: 1.314 ± 0.156
2.159PheArg: 2.159 ± 0.189
2.572PheSer: 2.572 ± 0.196
2.76PheThr: 2.76 ± 0.259
2.722PheVal: 2.722 ± 0.248
0.638PheTrp: 0.638 ± 0.133
1.427PheTyr: 1.427 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
3.586GlyAla: 3.586 ± 0.36
0.695GlyCys: 0.695 ± 0.125
3.755GlyAsp: 3.755 ± 0.268
3.83GlyGlu: 3.83 ± 0.268
3.06GlyPhe: 3.06 ± 0.263
3.717GlyGly: 3.717 ± 0.488
0.92GlyHis: 0.92 ± 0.149
4.881GlyIle: 4.881 ± 0.256
4.374GlyLys: 4.374 ± 0.335
5.313GlyLeu: 5.313 ± 0.374
1.671GlyMet: 1.671 ± 0.21
3.117GlyAsn: 3.117 ± 0.366
1.577GlyPro: 1.577 ± 0.167
2.065GlyGln: 2.065 ± 0.236
2.985GlyArg: 2.985 ± 0.219
4.431GlySer: 4.431 ± 0.345
4.487GlyThr: 4.487 ± 0.422
4.318GlyVal: 4.318 ± 0.293
1.164GlyTrp: 1.164 ± 0.143
2.948GlyTyr: 2.948 ± 0.234
0.0GlyXaa: 0.0 ± 0.0
His
1.126HisAla: 1.126 ± 0.165
0.263HisCys: 0.263 ± 0.078
1.239HisAsp: 1.239 ± 0.181
1.108HisGlu: 1.108 ± 0.137
0.92HisPhe: 0.92 ± 0.138
1.352HisGly: 1.352 ± 0.163
0.507HisHis: 0.507 ± 0.093
1.408HisIle: 1.408 ± 0.166
1.446HisLys: 1.446 ± 0.216
1.277HisLeu: 1.277 ± 0.136
0.413HisMet: 0.413 ± 0.097
0.695HisAsn: 0.695 ± 0.101
0.713HisPro: 0.713 ± 0.108
0.657HisGln: 0.657 ± 0.111
0.695HisArg: 0.695 ± 0.124
1.239HisSer: 1.239 ± 0.159
0.957HisThr: 0.957 ± 0.127
1.258HisVal: 1.258 ± 0.163
0.3HisTrp: 0.3 ± 0.075
0.901HisTyr: 0.901 ± 0.149
0.0HisXaa: 0.0 ± 0.0
Ile
4.769IleAla: 4.769 ± 0.368
0.789IleCys: 0.789 ± 0.106
4.787IleAsp: 4.787 ± 0.282
5.933IleGlu: 5.933 ± 0.353
2.272IlePhe: 2.272 ± 0.202
3.792IleGly: 3.792 ± 0.291
1.089IleHis: 1.089 ± 0.167
4.149IleIle: 4.149 ± 0.319
6.233IleLys: 6.233 ± 0.368
4.374IleLeu: 4.374 ± 0.293
1.896IleMet: 1.896 ± 0.206
3.924IleAsn: 3.924 ± 0.287
2.704IlePro: 2.704 ± 0.242
2.553IleGln: 2.553 ± 0.205
3.173IleArg: 3.173 ± 0.261
4.881IleSer: 4.881 ± 0.298
4.431IleThr: 4.431 ± 0.288
4.731IleVal: 4.731 ± 0.278
0.638IleTrp: 0.638 ± 0.094
2.384IleTyr: 2.384 ± 0.219
0.0IleXaa: 0.0 ± 0.0
Lys
6.102LysAla: 6.102 ± 0.449
0.807LysCys: 0.807 ± 0.126
4.45LysAsp: 4.45 ± 0.291
5.332LysGlu: 5.332 ± 0.391
3.192LysPhe: 3.192 ± 0.264
4.205LysGly: 4.205 ± 0.268
1.727LysHis: 1.727 ± 0.174
4.825LysIle: 4.825 ± 0.28
4.356LysLys: 4.356 ± 0.326
5.951LysLeu: 5.951 ± 0.369
2.61LysMet: 2.61 ± 0.217
3.361LysAsn: 3.361 ± 0.236
2.516LysPro: 2.516 ± 0.206
2.61LysGln: 2.61 ± 0.259
3.642LysArg: 3.642 ± 0.266
4.262LysSer: 4.262 ± 0.337
3.811LysThr: 3.811 ± 0.243
5.182LysVal: 5.182 ± 0.342
0.92LysTrp: 0.92 ± 0.111
3.023LysTyr: 3.023 ± 0.246
0.0LysXaa: 0.0 ± 0.0
Leu
6.158LeuAla: 6.158 ± 0.375
0.995LeuCys: 0.995 ± 0.156
5.482LeuAsp: 5.482 ± 0.369
5.332LeuGlu: 5.332 ± 0.296
3.248LeuPhe: 3.248 ± 0.296
4.224LeuGly: 4.224 ± 0.258
1.258LeuHis: 1.258 ± 0.134
5.032LeuIle: 5.032 ± 0.374
6.196LeuLys: 6.196 ± 0.408
4.487LeuLeu: 4.487 ± 0.278
2.347LeuMet: 2.347 ± 0.218
4.205LeuAsn: 4.205 ± 0.28
3.06LeuPro: 3.06 ± 0.228
2.553LeuGln: 2.553 ± 0.216
3.586LeuArg: 3.586 ± 0.276
4.731LeuSer: 4.731 ± 0.268
4.205LeuThr: 4.205 ± 0.311
4.468LeuVal: 4.468 ± 0.28
0.826LeuTrp: 0.826 ± 0.113
2.76LeuTyr: 2.76 ± 0.278
0.0LeuXaa: 0.0 ± 0.0
Met
2.647MetAla: 2.647 ± 0.205
0.282MetCys: 0.282 ± 0.067
1.389MetAsp: 1.389 ± 0.132
1.502MetGlu: 1.502 ± 0.174
1.277MetPhe: 1.277 ± 0.148
1.558MetGly: 1.558 ± 0.173
0.526MetHis: 0.526 ± 0.118
2.272MetIle: 2.272 ± 0.223
2.516MetLys: 2.516 ± 0.22
2.028MetLeu: 2.028 ± 0.179
0.713MetMet: 0.713 ± 0.11
1.765MetAsn: 1.765 ± 0.166
0.901MetPro: 0.901 ± 0.115
1.07MetGln: 1.07 ± 0.137
1.183MetArg: 1.183 ± 0.125
2.065MetSer: 2.065 ± 0.223
1.877MetThr: 1.877 ± 0.196
1.633MetVal: 1.633 ± 0.168
0.225MetTrp: 0.225 ± 0.061
1.202MetTyr: 1.202 ± 0.16
0.0MetXaa: 0.0 ± 0.0
Asn
3.605AsnAla: 3.605 ± 0.282
0.713AsnCys: 0.713 ± 0.115
2.722AsnAsp: 2.722 ± 0.231
3.567AsnGlu: 3.567 ± 0.266
2.497AsnPhe: 2.497 ± 0.209
4.243AsnGly: 4.243 ± 0.367
1.014AsnHis: 1.014 ± 0.131
3.623AsnIle: 3.623 ± 0.237
3.811AsnLys: 3.811 ± 0.242
3.699AsnLeu: 3.699 ± 0.275
1.408AsnMet: 1.408 ± 0.163
2.553AsnAsn: 2.553 ± 0.27
2.065AsnPro: 2.065 ± 0.175
1.802AsnGln: 1.802 ± 0.212
2.046AsnArg: 2.046 ± 0.178
3.192AsnSer: 3.192 ± 0.308
2.779AsnThr: 2.779 ± 0.26
3.21AsnVal: 3.21 ± 0.232
0.789AsnTrp: 0.789 ± 0.135
2.009AsnTyr: 2.009 ± 0.169
0.0AsnXaa: 0.0 ± 0.0
Pro
2.872ProAla: 2.872 ± 0.24
0.357ProCys: 0.357 ± 0.083
2.197ProAsp: 2.197 ± 0.263
3.098ProGlu: 3.098 ± 0.232
1.446ProPhe: 1.446 ± 0.161
2.854ProGly: 2.854 ± 0.248
0.544ProHis: 0.544 ± 0.106
2.103ProIle: 2.103 ± 0.19
2.309ProLys: 2.309 ± 0.182
2.103ProLeu: 2.103 ± 0.223
0.732ProMet: 0.732 ± 0.106
1.708ProAsn: 1.708 ± 0.171
0.901ProPro: 0.901 ± 0.158
0.939ProGln: 0.939 ± 0.144
1.464ProArg: 1.464 ± 0.171
2.384ProSer: 2.384 ± 0.203
1.84ProThr: 1.84 ± 0.212
2.553ProVal: 2.553 ± 0.212
0.695ProTrp: 0.695 ± 0.121
1.126ProTyr: 1.126 ± 0.138
0.0ProXaa: 0.0 ± 0.0
Gln
3.004GlnAla: 3.004 ± 0.256
0.338GlnCys: 0.338 ± 0.071
1.99GlnAsp: 1.99 ± 0.22
2.178GlnGlu: 2.178 ± 0.191
1.727GlnPhe: 1.727 ± 0.193
1.765GlnGly: 1.765 ± 0.188
0.751GlnHis: 0.751 ± 0.137
2.553GlnIle: 2.553 ± 0.249
2.065GlnLys: 2.065 ± 0.234
2.91GlnLeu: 2.91 ± 0.21
1.033GlnMet: 1.033 ± 0.135
1.464GlnAsn: 1.464 ± 0.164
0.826GlnPro: 0.826 ± 0.128
0.957GlnGln: 0.957 ± 0.134
1.84GlnArg: 1.84 ± 0.157
2.028GlnSer: 2.028 ± 0.193
1.859GlnThr: 1.859 ± 0.194
2.591GlnVal: 2.591 ± 0.201
0.732GlnTrp: 0.732 ± 0.123
1.934GlnTyr: 1.934 ± 0.205
0.0GlnXaa: 0.0 ± 0.0
Arg
3.117ArgAla: 3.117 ± 0.236
0.62ArgCys: 0.62 ± 0.099
2.891ArgAsp: 2.891 ± 0.242
3.267ArgGlu: 3.267 ± 0.23
2.084ArgPhe: 2.084 ± 0.22
2.591ArgGly: 2.591 ± 0.195
0.995ArgHis: 0.995 ± 0.142
3.398ArgIle: 3.398 ± 0.224
3.511ArgLys: 3.511 ± 0.269
3.661ArgLeu: 3.661 ± 0.28
1.371ArgMet: 1.371 ± 0.154
2.459ArgAsn: 2.459 ± 0.224
1.202ArgPro: 1.202 ± 0.161
1.84ArgGln: 1.84 ± 0.173
2.122ArgArg: 2.122 ± 0.251
2.459ArgSer: 2.459 ± 0.212
2.384ArgThr: 2.384 ± 0.231
3.023ArgVal: 3.023 ± 0.209
0.826ArgTrp: 0.826 ± 0.118
2.009ArgTyr: 2.009 ± 0.204
0.0ArgXaa: 0.0 ± 0.0
Ser
4.619SerAla: 4.619 ± 0.382
0.751SerCys: 0.751 ± 0.137
4.299SerAsp: 4.299 ± 0.259
4.168SerGlu: 4.168 ± 0.238
2.647SerPhe: 2.647 ± 0.233
4.806SerGly: 4.806 ± 0.317
1.033SerHis: 1.033 ± 0.131
4.299SerIle: 4.299 ± 0.307
4.731SerLys: 4.731 ± 0.389
4.6SerLeu: 4.6 ± 0.301
1.596SerMet: 1.596 ± 0.173
3.079SerAsn: 3.079 ± 0.345
2.028SerPro: 2.028 ± 0.236
2.065SerGln: 2.065 ± 0.194
2.966SerArg: 2.966 ± 0.224
4.881SerSer: 4.881 ± 0.365
3.849SerThr: 3.849 ± 0.264
3.943SerVal: 3.943 ± 0.286
0.901SerTrp: 0.901 ± 0.124
2.553SerTyr: 2.553 ± 0.193
0.0SerXaa: 0.0 ± 0.0
Thr
3.83ThrAla: 3.83 ± 0.301
0.394ThrCys: 0.394 ± 0.08
3.06ThrAsp: 3.06 ± 0.23
3.699ThrGlu: 3.699 ± 0.28
2.628ThrPhe: 2.628 ± 0.237
4.205ThrGly: 4.205 ± 0.346
1.202ThrHis: 1.202 ± 0.133
3.868ThrIle: 3.868 ± 0.241
3.586ThrLys: 3.586 ± 0.256
4.374ThrLeu: 4.374 ± 0.369
1.577ThrMet: 1.577 ± 0.171
2.948ThrAsn: 2.948 ± 0.284
2.366ThrPro: 2.366 ± 0.27
2.009ThrGln: 2.009 ± 0.244
2.76ThrArg: 2.76 ± 0.222
3.736ThrSer: 3.736 ± 0.371
3.248ThrThr: 3.248 ± 0.384
4.543ThrVal: 4.543 ± 0.402
0.638ThrTrp: 0.638 ± 0.099
2.441ThrTyr: 2.441 ± 0.216
0.0ThrXaa: 0.0 ± 0.0
Val
4.149ValAla: 4.149 ± 0.28
0.695ValCys: 0.695 ± 0.119
4.487ValAsp: 4.487 ± 0.283
5.614ValGlu: 5.614 ± 0.349
2.647ValPhe: 2.647 ± 0.244
3.849ValGly: 3.849 ± 0.298
1.07ValHis: 1.07 ± 0.141
4.787ValIle: 4.787 ± 0.311
5.238ValLys: 5.238 ± 0.316
5.032ValLeu: 5.032 ± 0.343
1.859ValMet: 1.859 ± 0.179
3.511ValAsn: 3.511 ± 0.246
2.516ValPro: 2.516 ± 0.187
2.666ValGln: 2.666 ± 0.219
3.379ValArg: 3.379 ± 0.244
4.431ValSer: 4.431 ± 0.286
3.548ValThr: 3.548 ± 0.341
5.313ValVal: 5.313 ± 0.34
0.845ValTrp: 0.845 ± 0.122
2.647ValTyr: 2.647 ± 0.192
0.0ValXaa: 0.0 ± 0.0
Trp
0.92TrpAla: 0.92 ± 0.115
0.225TrpCys: 0.225 ± 0.06
0.807TrpAsp: 0.807 ± 0.104
0.845TrpGlu: 0.845 ± 0.13
0.695TrpPhe: 0.695 ± 0.12
0.544TrpGly: 0.544 ± 0.109
0.357TrpHis: 0.357 ± 0.093
0.864TrpIle: 0.864 ± 0.131
1.22TrpLys: 1.22 ± 0.182
1.126TrpLeu: 1.126 ± 0.145
0.507TrpMet: 0.507 ± 0.098
0.77TrpAsn: 0.77 ± 0.123
0.507TrpPro: 0.507 ± 0.086
0.469TrpGln: 0.469 ± 0.088
0.563TrpArg: 0.563 ± 0.091
0.77TrpSer: 0.77 ± 0.124
0.976TrpThr: 0.976 ± 0.164
1.183TrpVal: 1.183 ± 0.159
0.244TrpTrp: 0.244 ± 0.08
0.845TrpTyr: 0.845 ± 0.123
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.779TyrAla: 2.779 ± 0.24
0.732TyrCys: 0.732 ± 0.121
2.854TyrAsp: 2.854 ± 0.227
2.178TyrGlu: 2.178 ± 0.211
1.652TyrPhe: 1.652 ± 0.159
2.666TyrGly: 2.666 ± 0.255
0.826TyrHis: 0.826 ± 0.134
2.591TyrIle: 2.591 ± 0.176
2.966TyrLys: 2.966 ± 0.26
3.06TyrLeu: 3.06 ± 0.222
1.108TyrMet: 1.108 ± 0.156
2.591TyrAsn: 2.591 ± 0.205
1.84TyrPro: 1.84 ± 0.178
1.483TyrGln: 1.483 ± 0.186
1.934TyrArg: 1.934 ± 0.175
2.741TyrSer: 2.741 ± 0.236
2.234TyrThr: 2.234 ± 0.198
2.854TyrVal: 2.854 ± 0.25
0.695TyrTrp: 0.695 ± 0.119
1.652TyrTyr: 1.652 ± 0.197
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 287 proteins (53265 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski