Streptosporangiales bacterium
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5548 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6N7I6E9|A0A6N7I6E9_9ACTN ATP-binding cassette domain-containing protein OS=Streptosporangiales bacterium OX=2604148 GN=GEV03_01750 PE=4 SV=1
MM1 pKa = 7.78 HH2 pKa = 8.41 DD3 pKa = 5.08 DD4 pKa = 3.77 TPQDD8 pKa = 3.68 PFRR11 pKa = 11.84 DD12 pKa = 4.0 DD13 pKa = 4.15 PFDD16 pKa = 4.23 PSAALGDD23 pKa = 4.48 DD24 pKa = 3.66 EE25 pKa = 6.95 DD26 pKa = 4.43 EE27 pKa = 4.99 ARR29 pKa = 11.84 QLDD32 pKa = 3.86 PAEE35 pKa = 4.05 QEE37 pKa = 4.28 EE38 pKa = 4.63 VLSDD42 pKa = 3.72 LADD45 pKa = 3.77 LEE47 pKa = 4.54 VFRR50 pKa = 11.84 ALLEE54 pKa = 4.15 PQGVKK59 pKa = 10.52 GMAIDD64 pKa = 4.26 CADD67 pKa = 4.07 CGEE70 pKa = 4.03 VHH72 pKa = 6.45 YY73 pKa = 10.82 LGWDD77 pKa = 3.56 LLHH80 pKa = 7.0 GNLRR84 pKa = 11.84 QLLEE88 pKa = 4.13 NGGQQVHH95 pKa = 6.15 EE96 pKa = 4.6 PACGPDD102 pKa = 3.2 PSEE105 pKa = 4.05 YY106 pKa = 9.57 VTWDD110 pKa = 3.31 YY111 pKa = 11.93 ARR113 pKa = 11.84 GYY115 pKa = 9.73 VDD117 pKa = 4.85 GVIDD121 pKa = 3.83 SEE123 pKa = 4.34 EE124 pKa = 4.38 SKK126 pKa = 11.18 GYY128 pKa = 10.61
Molecular weight: 14.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.478
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.63
EMBOSS 3.719
Sillero 3.91
Patrickios 1.875
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.814
Protein with the highest isoelectric point:
>tr|A0A6N7IBJ8|A0A6N7IBJ8_9ACTN ComF family protein OS=Streptosporangiales bacterium OX=2604148 GN=GEV03_10235 PE=4 SV=1
MM1 pKa = 7.46 GSIIKK6 pKa = 10.03 KK7 pKa = 8.4 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.52 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.51 LLKK22 pKa = 8.15 KK23 pKa = 9.25 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.81 KK32 pKa = 9.63
Molecular weight: 4.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.735
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.457
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5548
0
5548
1684724
26
1560
303.7
32.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.829 ± 0.048
0.866 ± 0.01
5.905 ± 0.024
6.084 ± 0.032
2.904 ± 0.02
9.482 ± 0.031
2.178 ± 0.017
3.582 ± 0.022
1.724 ± 0.021
10.357 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.841 ± 0.013
1.746 ± 0.014
6.025 ± 0.031
2.655 ± 0.018
8.889 ± 0.032
4.856 ± 0.021
5.387 ± 0.019
8.952 ± 0.032
1.508 ± 0.014
2.229 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here