Anaplasma marginale (strain Florida)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; Anaplasma; Anaplasma marginale

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 939 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9KHG5|B9KHG5_ANAMF Uncharacterized protein OS=Anaplasma marginale (strain Florida) OX=320483 GN=AMF_029 PE=4 SV=1
MM1 pKa = 7.66FDD3 pKa = 4.19FDD5 pKa = 3.84NTYY8 pKa = 11.56DD9 pKa = 3.48LAFRR13 pKa = 11.84IDD15 pKa = 4.06DD16 pKa = 4.18ADD18 pKa = 3.59LSRR21 pKa = 11.84NFSVSDD27 pKa = 2.97GHH29 pKa = 6.76VVVTNGDD36 pKa = 3.09IFAGHH41 pKa = 6.6SPLQHH46 pKa = 6.5CNAPAQEE53 pKa = 4.0PMSYY57 pKa = 8.7CTSHH61 pKa = 6.4VVLGRR66 pKa = 11.84DD67 pKa = 3.68GEE69 pKa = 4.61VTADD73 pKa = 3.6IYY75 pKa = 11.71NSALTGIALNIASDD89 pKa = 3.73KK90 pKa = 10.94KK91 pKa = 10.77SLEE94 pKa = 3.73AHH96 pKa = 6.49FSINPLQALPVMSQMYY112 pKa = 10.22PEE114 pKa = 4.49VQVQVYY120 pKa = 8.04TMADD124 pKa = 3.03YY125 pKa = 11.48DD126 pKa = 4.97FFDD129 pKa = 4.83EE130 pKa = 6.49DD131 pKa = 5.95EE132 pKa = 4.4DD133 pKa = 4.34LQWW136 pKa = 5.35

Molecular weight: 15.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B9KJ45|B9KJ45_ANAMF Phenylalanine--tRNA ligase beta subunit OS=Anaplasma marginale (strain Florida) OX=320483 GN=pheT PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84IVRR12 pKa = 11.84KK13 pKa = 9.26RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTRR25 pKa = 11.84WGRR28 pKa = 11.84KK29 pKa = 6.43ILNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.26GRR39 pKa = 11.84CLLCAA44 pKa = 4.4

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

939

0

939

342329

44

3493

364.6

39.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.081 ± 0.113

1.915 ± 0.042

5.153 ± 0.068

5.903 ± 0.071

3.883 ± 0.064

7.801 ± 0.077

2.327 ± 0.045

5.806 ± 0.071

5.124 ± 0.07

9.315 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.678 ± 0.041

3.368 ± 0.045

3.8 ± 0.063

2.985 ± 0.064

5.662 ± 0.055

7.937 ± 0.073

4.728 ± 0.063

8.735 ± 0.082

0.854 ± 0.024

2.945 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski